BHLHB5 (BHLHE22) (AK091333) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Beta3; BHLHB5; CAGL85; TNRC20
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK091333, the custom clone sequence may differ by one or more nucleotides CTTTGCCTCAAGTACGGCGAAAGCGCGAGCCGGGGCTCGGTGGCCGAGAGCAGCGGCGGCGAGCAGAGCC CCGACGACGACAGCGACGGTCGCTGCGAGCTCGTGCTGCGGGCCGGAGTAGCCGACCCGCGGGCCTCCCC GGGAGCGGGAGGTGGTGGCGCGAAGGCAGCCGAGGGCTGCTCCAATGCCCACCTCCACGGCGGCGCCAGC GTCCCCCCGGGGGGCCTGGGCGGCGGCGGCGGCGGGGGTAGCAGCAGCGGTAGCAGTGGCGGCGGTGGCG GTAGCGGTAGCGGCAGCGGCGGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAAGAAATCCAAAGAGCAAAA GGCGCTGCGGCTTAACATCAATGCCCGAGAGCGCCGGCGGATGCACGACCTGAACGGCGCGCTGGACGAG CTGCGCGCGGTGATCCCCTACGCGCACAGCCCCTCGGTGCGAAAGCTCTCCAAGATCGCCACGCTGCTGC TCGCCAAGAACTACATCCTCATGCAGGCGCAGGCCCTGGAGGAGATGCGGCGCCTAGTCGCCTACCTCAA CCAGGGCCAGGCCATCTCGGCTGCCTCCCTGCCCAGCTCGGCGGCTGCAGCGGCAGCAGCTGCTGCCCTG CACCCGGCGCTCGGCGCCTACGAGCAGGCAGCCGGCTACCCGTTCAGCGCCGGACTGCCCCCGGCTGCCT CCTGCCCGGAGAAGTGCGCCCTGTTTAACAGCGTCTCCTCCAGCCTCTGCAAACAGTGCACGGAGAAGCC TTAAACACACCCCCGAAAAACACAAGACCGACCCAAAATCTAGAGGAAAGCGAAAAGCTGCTCCCCACCC CCTTTATTTTGGTCCTCTCGTAGTTGTGAAACACTTGCAGAGCAAACAAAGCAGAGGCAAGAACTGAGGA GAAGTATCAGAGACAAACGGGACTTTTAGCCTTGACATCCCCAGAATCTCGGTCTTTGGGGTGGGGAGGG AGGGAGGAGGGAGGTGGAGTTGGGATGGAGTATGGATGTCTTTTTTTTCTCAGAAAAGTGGCAACTTTGG TGGCAGCCTAGACCGCGAGGAAGTGGAATCTTCCTTAAAGGTGAAACATAGTTGAGAAGTAGTGCTTGGT TATTAAGCCTGGAGAGTGTTTGAATGGCAAAATACTAATCCTACTAATAATTGTGGATTTGGCTAGTGCT GAGGGGGAGAGGAGGGGTTAGGGGTTGGGTAGCATGAGAGATGGAATATTCATTCAGACAAATCAGAAAG GGCACTTGAAAATAAATTTTGATTCTGAGCCAGGAGAAAAACTTGAAATGTTGACTTAAGTGAGGGGGAA ATAAAACCCAGAGAGAGAGAGAGAGCGAGAGAGAGAGACATGTTACTATGAAAGACTTGTATTTTTTATT GTCTCTGAACTTTAATCCTGTGAAAATGCTCAAAGTTTTGGCGAGAGTGATATAAAAAAACTGACTGTGG CTGAAAGAATTGCATTTAAAAATATTTATGTGCACCTTGTTACTTTCTACTCTGCAATGATGTATTAACA ATTCATGGTATTTATTTGTTATTCTTCAATGACCCTTCCACATCAACAGTATTTATCATGTGTTAAAGTC ATGGGTCTTATGTGCAGATTTTTTTAATGCCTCTAAAATTGTTTTATAGATTACTTATGCTGTGTGCCAA GTGTTTAAAGGTTTATTTGCCAACAAGTATGAAGAGAAATATTGATGTATCTGAGCTGCTTAGGTTTATG AAAGGTCACCTGGATATTTTCAGTTGCATCATCCCTGTATTTAAATTGAGCTTTAATAAATTGCTTCAGT CCAAAAATTACAGGAAAGGTGTATTCTTAATTGATGAATGAATTGATCTCTTTTTTGAAAGGGGACTCCT TTACATTCCAAAAGGGAAAAACATCACAGCAATAGTTGCTATACGTAAGAACATGCTAAGCAAATATTTT TCCAGGCTGTGCTGTGCATTTTTAAAAGGTCTAATTTAATTGCTTTTAATATATATGTACATATATGTTA TTTTAACTGTGGAGAATTATTTAAGTTAAAAGACTGGTTTGATTTGCCTATGGTGTGAAATCCTTTGTTA TTTTTCTAAAAAAATAAAATTTAAAAAGAAAGAAAACTAAGGAAGAACAAGAAGCTATTTACCCAAAGTG AGCTTTCAGTTTTAGTTTTGCATGGCTGTTTGACTGCCTTTCCGCCCTATGAAAATCAAGAAAATCTTTT TTAAAAAATGGAGTCCTGCTATTTTCCACTCCTTGCAGATAATACAAATTCAGTTTGTCAGGTTGGATGG TGAGTTGGGAGCTGTGATGGATCTGTTGGCGGGTTTTGGATGTGTAAAGAATGATATATATATTAAATAG GTCAATCAGACTATGACAGCTATGTACGACCATTTGTATGTGTATCTATGTCAGAAAGAATCTTTATTAA AATATTTTGATCAAAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK091333 unedited CAAAATTTGTATACGAACTCACTATAGGGCGGCCGCGATTCGGCACGAGGGGTGGGTGTC CCCGGGCTGCTAGTAGGTTCAGCCGGCGTTGGGGGCGACCCTAGCCTAAGCAGCCTGCCG GCCGGGGCCGCCCTTTGCCTCAAGTACGGCGAAAGCGCGAGCCGGGGCTCGGTGGCCGAG AGCAGCGGCGGCGAGCAGAGCCCCGACGACGACAGCGACGGTCGCTGCGAGCTCGTGCTG CGGGCCGGAGTAGCCGACCCGCGGGCCTCCCCGGGAGCGGGAGGTGGTGGCGCGAAGGCA GCCGAGGGCTGCTCCAATGCCCACCTCCACGGCGGCGCCAGCGTCCCCCCGGGGGGCCTG GGCGGCGGCGGCGGCGGGGGTAGCAGCAGCGGTAGCAGTGGCGGCGGTGGCGGTAGCGGT AGCGGCAGCGGCGGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAAGAAATCCAAAGAGCAA AAGGCGCTGCGGCTTAACATCAATGCCCGAGAGCGCCGGCGGATGCACGACCTGAACGAC GCGCTGGACGAGCTGCGCGCGGTGATCCCCTACGCGCACAGCCCCTCGGTGCGAAAGCTC TCCAAGATCGCCACGCTGCTGCTCGCCAAGAACTACATCCTCATGCAGGCGCAGGCCCTG GAGGAGATGCGGCGCCTAGTCGCCTACCTCAACCAGGGCCAGGCCATCTCGGCTGCCTCC CTGCCCAGCTNCGCGGCTGCAGCGGCAGCAGCTGCTGCCCTGCACCCGGCGCTCGGCGCC TACGAGCAGGCAGCCGGCTACCCGTTCAGCGCCGGACTGCCCCCGGCTGCCTNCTGCCNG NAGAAGTGCGCCCTGTTTAACAGCGTCTNCTTCAGCCTCTNGCAACAGTGCACGGNAGAA GCCTTAAACACACCCCCGAANAACACAGACCGACCCAAATCTAGAGGAAAGCGAAAGCTG CTCCCACCCCTT
Restriction Sites	NotI-NotI
ACCN	AK091333
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK091333.1
RefSeq Size	2466 bp
RefSeq ORF	2466 bp
Locus ID	27319
Domains	HLH
Protein Families	Druggable Genome
Gene Summary	This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors that regulate cell fate determination, proliferation, and differentiation. A similar protein in mouse is required for the development of the dorsal cochlear nuclei, and is thought to play a role in in the differentiation of neurons involved in sensory input. The mouse protein also functions in retinogenesis. [provided by RefSeq, Oct 2016]

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