RICH2 (ARHGAP44) (AK023797) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NPC-A-10; RICH2
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK023797, the custom clone sequence may differ by one or more nucleotides ACAACTTTGTGTTTTGTTGTTGTGTTGTGTTTTCTCTTTCAGTTTCAAAGAAGCTGGCACCGATTCCACC CAAGGTCCCCTTTGGCCAGCCGGGGGCTATGGCAGACCAGTCCGCTGGCCAGCCGTCCCCAGTCAGCCTG TCCCCCACCCAGCCCAGCACCCCGTCACCCTATGGACTGAGCTACCCTCAGGGGTACTCCTTGGCCTCGG GCCAGCTCTCCCCAGCTGCAGCTCCTCCCCTGGCCTCTCCTTCTGTCTTTACAAGCACTTTGAGCAAATC GCGGCCCACTCCTAAGCCGCGACAGAGACCTACTCTGCCGCCTCCTCAGCCTCCCACAGTAAACCTCTCG GCCTCTAGTCCACAGTCCACGGAGGCCCCCATGCTAGATGGCATGTCCCCTGGGGAAAGCATGTCTACAG GTAACCAAGCCACAGGCTCCCTCACTTTTTTTTATGAACTTTAGTGAAATGTGCCTAGGGGATGGATTGC TAGAGTTACTGCCAGTGAACACATTCCTGCGTGCTTTGGGATGACTGGGCTGGCACCAGGGGTAGCTCAT AAGATCTCCTTAGAAGCCCACCAATGGAAAGCAACCCTGGAATCCATGTGTCCCACACAGGGGCTTCTTA TGGGATCTCTCTCGGTCTAAGCCCACCAGCCTGGGGTTCTGTTTTTGCTCTTATTATCGGGGGTTGAAGA CACTGAGTCATTTGGAGTGGGGCCTCAGGAGAGGCTGGAAGCTGGTTATGATCACAAAACAGCATCAAAA CTCAGGTATTTCCTGGATAGAGCCCAAGCAACTGACAGGTATAAATGCTTATCTGGAGAACAGGGGCTAA GAACCCAAGATGTTTTTTTACTATTACTAAAATAAGCCTTGCTAGAAAAATATACTTCCAGATAGATATT TAGAGAGGGGTGAATTTCAGAAAACATGCATGTCTCCATTCTCCTCCACCAGACTCCCCTCCCTGGCCAA TTCCCTGAGGAGCTAATTGGACAAACCCACAACGACTTAATTTAGTTTGGGCTTAAATTTAGAAACAAAG ACGGAGCTCTCTTCCCCCAGAAAAATCTGTAAGCACAAAATCGCAAACACACACCACGTTTAGCCAAAAC CATTCATTGGCTGGAAGGAACGGGATGTTTTTGGAACATCTCCCCAGACACTTCAACCACATAAGATTAT AAAGTCAGGGGCCTATTTGTGGTGGCTGCTAAAGTCATTTGAGCATTTTCCTTGTTCAGGGAGACAGGCA CAGGGTTGAGCCGTGTCTTGGGCTAGAGCCAACCCACTTAGTAAAGTGCAGTAGCATGGCGGTGAGCTGG AGCATATGTGTACCTAGTGTTGTCCCAAGTAGCAGAAGGTGAGGCAGCTTGGGTTGGAACACACACACCA CATACACACACACCCACTCACCCATTTCAAAGAACAGTGCTGTCCTAGGATGCACTGGGACTTTTGTGAT AATGCACTTTTCCCTCCACGTCCTTCTCCAAAAGAGTGATTTATTTCTTCCGTGATTAGACGGAAGCAGT GACCCTGTGGGGTTCAGCTCCGTGCTGCCTTTGAAAAGGCTAGTCCAATTTGCAAGTGTTTGTACTGGGT CAGATTTAAATATTACGGGGAAGAGCCATCTGGTGATTCGGCTTCTTTATTTTTGGATGTGATTAATGTT GAAGGTACAAGCTGCTGTCATCTACCCCGAGGCTTATTAAAAATTAGGCAAATTTCTCCCTGGAAAATGC AGTAGCACCACGCCCATCTTGGTGGGTCTTGGTGCTGAGGTGTCTCCTGAAGGAGCCCATGATAGGAGGG CACAGGCTTGCTGCCCTCGGGGAAGGTCACAGCTGCGGAGGAGCTGAAGGTCACCCGAGCCAGGTCTCCA GGGCTGCTCCTCCCAGTGTGTCACATCTGGTGACTCTTCCAGCCACCCCAGGGAAGGGACACAAGGTAGT GTCAATACATAAGCAATAGAATGAGCAGAAGATGGCCAGGCACGGTGGCTCACGCCTGTAATCCCAGCAC TTTGGGAGGCCGAGGAGAATTAGTTGGGCATGGTGGCACGTGCCACTAGTCCCAGCTACTTGGGAGGCTG AGGTAGGAGAATTGCTTGAACCTGGGAGGTGGAGCCGAGATTGTGCCACTGCATTCCAGCCTGGCAACAG AGCGATACTCCACCTCAAAAAAAAAAAAAAAAAAAAAAAAAGAATGAGCAGAAGATATCAATGGAGAAGC AGATAAGAGCAGAAGGAGAGGAGAAATTGGGGAACAAAAGTGGGCAACAGAAGGCCTGGGAGGACAGCAC CCCCAGGGCTCTAAGAAGCGGAGTGGCTCATGTTCCATCTTACAGGTTTAAGAACTCCTGACCTTCAGGA TTCATAATGTCCCTCGCCCTGTGCGGACCCTATTTTAATGTCCTTTTGGCAGCAGACTGTTGTTTTGTCC CATACGTTCAGGTCTCTGAGCCAGTGTAGAATCCATCATGGTTTGATGTGGCCCGTCTGGGACTGTGATA TTGGCATAGCTTTTTTTTTTTTTTGTGACGTTCATGTTTTGTCGTGTTGATTCCTTTCTCCGAATCCTAT CATTGTGTTCTTGCAGCTGTGTGACATGAGTGGCGCAGTTTTGGATACGTGTGAGGGGGATTTTCAGGGT AAGCTGGCCCCGGCCTCGCCCCTCCACCCCGCTAGCTAGCCAGGCCAGCTGCCCGCTCCTCCCCTCCGCT CCTCGTCTCTGCATGTGGCTCAGACCATTTGTATGGCAGCTTCACTCTCCAGCCTTCCCCGGCCTCAGCA AGGATGAGTTGATCCATGAGCTTATTTGGCTTTGGGGCAGCCCTGGCCTCCTTTTCTTTCTTTTCATTAG GAGGAGCTGGGGGAGAACCCAGCCAGCCCCTTTTCCCCACCTTCTCTTTCACTGGAATCACATCTGCAGC TCCTCCCCCTCCGCCCTCCCTCAGGCTTAGACCGTGGAGCTGTAACAGATCTGTTCTTCGTGTGAGACTT TTGTGCTGGAGACGCTTGTGCCCAGCACCCTGGGGGACATGCACCTGCTCACCTTTGTTTAGGAAGGCGT CCTGAGGGGAGGTCATACCAGGTGCAGCCCAGCACAGGGTCCACACCACAGCTTGGACCCCATTTTCTGT GGCCCCGAGTTTGCACCAGCAGATCTCCCCTTCCAGACTTAGATCCTAACGTCTGGCTTCCTCCAGGCTG AGACTGCTGGCAAATCCTAAAGAATCCATATCGGAGCTCATCCCGCTCCCCTTCTCCCTCATCCCAAGTG GATATGGCCAGACAGATCCTTTCTATCCAACTCAATAAAATTTGGGGGAGCATTTATATCAGTATCTATT TGGTCTCCTCCTCCTATAAAGAGTAAATCCTCAAAGCATAACTGAGGGAAAGGCACGAAGGAAGCCAGAA GGACTTTGGTGTATTTGCCTCAGTAAGTAGAGGTGGCCAAAGTAAACCAGGTCTTCTCCCCGATTTGGCC CATGGTCCTGCAGAGACTACCGAGGCGCACCAGGATATCTGGTGCTCACAGACAGGTCTCCTGGCGGGTG CTTCCCGAGTGTCCTGGAGACAAGCAGCCACTGTGTCCCTTTTCCCAGGAATGCTGATGAGGGCTTCTGC CCCTGAATCCCATTCCCTTGCATAGTGACTCCTCCCTTGGGGGTGTCTTTAAGGGAGCAGCAAGGATAAA GGTATGACAGCTCACACAAGTGCTTGTTCTGGGCCTGTCTAAATTCATCACCGCCCTGTATTATTCCCAT TTGACTGCTGAGGCACAGGGAAGGTAGGAAACCTGTCCAAGGTCATGCTCTTCCTGCCTGACATATTTGG GATTTTTGAATCTAAGCAGGGGAGCTTGGTGCTCTTACCTGATAGCGAACCCTGCACAGTGAGGTGGGCC CCGGGCCACTCAGCCTCACACCTGTGTAGAGACATGACCCAAGTAGCTGGGGAAGGGCAGAGGTGGTCTG AGGCCACCTGCTTCAGAGGAACAAGTGGCACTGGCTGTTCCTCCAGCCCCACGCCCCAAATCTCTGCTTC TCCATCAGGCAGCAAAACAAAGACTGGAAGGACGTGAGGGCTGCCTGGGGGTTGGGGCAAGGGAGGACCT TTGTTCAAGAGCTTCCATGTTTGTATGATGTCTTTCAGGACAGTGTCCAAATGTATGTCTGTACACTTAG TATGGTATTCCCCAGTCTGAACATGTGTTCCCTTCCTCTGCCTCCTGGAGTGCTGCTCCCAGCCTTGCAA AGCTAAGTGACAGCCAGAAACTAAGCAGGCAGGCCGGGCGTGGTTGCTCACGCCTGTAATCCCAAAACTT TGGGAGGCTGAAGTGGGTGAATCACCTGAGGTCACGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACC CAATCTCTACTAAAAACACC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK023797 unedited GGTCAACATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCAGGCTCCA GCCAGGGCACAGCCTGTGCAGGGACTCAACCAGGGGCTCAACCTGGAGCTCAGCCGGGCG CCAGCCCCAGCCCCAGCCAGCCGCCTGCAGACCAGAGTCCTCACACCCTCCGGAAAGTTT CAAAGAAGCTGGCACCGATTCCACCCAAGGTCCCCTTTGGCCAGCCGGGGGCTATGGCAG ACCAGTCCGCTGGCCAGCCGTCCCCAGTCAGCCTGTCCCCCACCCCGCCCAGCACCCCGT CACCCTATGGACTGAGCTACCCTCAGGGGTACTCCTTGGCCTCGGGCCAGCTCTCCCCAG CTGCAGCTCCTCCCCTGGCCTCTCCTTCTGTCTTTACAAGCACTTTGAGCAAATCGCGGC CCACTCCTAAGCCGCGACAGAGACCTACTCTGCCGCCTCCTCAGCCTCCCACAGTAAACC TCTCGGCCTCTAGTCCACAGTCCACGGAGGCCCCCATGCTAGATGGCATGTCCCCTGGGG AAAGCATGTCTACAGGTAACCAAGCCACAGGCTCCCTCACTTTTTTTTATGAACTTTAGT GAAATGTGCCTAGGGGAGGGATTGCTAGTGTTACTGCCAGTGAACACATTCCTGCGTGCT TTGGGATGACTGGGCTGGCACCAGGGGTAGCTCATAAGATCTCCTTAGAAGCCCACCAAT GGAAAGCAACCCTGGAATCCATGTGTCCCACACAGGGGCTTCTTATGGGATCTCTCTCGG TCTAANGCCACCAGCCTGGGGGTNCTGTTTTTGCTCTTATATCGGGGGNTNGAGACACTG AGTCATTTGGAGTGGGGCCTCNAGAGAGGCTGAAGCTGGNTATGATCACAAACAGCATCA AACTCAGGTTTTNCTGGNAAGAGCCCAGCACTGACAGTT
Restriction Sites	NotI-NotI
ACCN	AK023797
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK023797.1
RefSeq Size	4430 bp
RefSeq ORF	4430 bp
Locus ID	9912
Gene Summary	GTPase-activating protein (GAP) that stimulates the GTPase activity of Rho-type GTPases. Thereby, controls Rho-type GTPases cycling between their active GTP-bound and inactive GDP-bound states. Acts as a GAP at least for CDC42 and RAC1 (PubMed:11431473). In neurons, is involved in dendritic spine formation and synaptic plasticity in a specific RAC1-GAP activity (By similarity). Limits the initiation of exploratory dendritic filopodia. Recruited to actin-patches that seed filopodia, binds specifically to plasma membrane sections that are deformed inward by acto-myosin mediated contractile forces. Acts through GAP activity on RAC1 to reduce actin polymerization necessary for filopodia formation (By similarity). In association with SHANK3, promotes GRIA1 exocytosis from recycling endosomes and spine morphological changes associated to long-term potentiation (By similarity).[UniProtKB/Swiss-Prot Function]

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