MRPL32 (AK090551) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bMRP-59b; HSPC283; L32mt; MRP-L32
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK090551, the custom clone sequence may differ by one or more nucleotides ATGGCGCTGGCCATGCTGGTCTTGGTGGTTTCGCCGTGGTCTGCGGCCCGGGGAGTGCTTCGAAACTACT GGGAGCGACTGCTACGGAAGCTTCCGCAGAGCCGGCCGGGCTTTCCCAGTCCTCCGTGGGGACCAGCATT AGCAGTACAGGGCCCAGCCATGTTTACAGAGCCAGCAAATGATACCAGTGGAAGTAAAGAGAATTCCAGC CTTTTGGACAGTATCTTTTGGATGGCAGCTCCCAAAAATAGACGCACCATTGAAGTTAACCGGTGTAGGA GAAGAAATCCGCAGAAGCTTATTAAAGTTAAGGTAATGCATTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK090551 unedited GGGGTGCACCATTATGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGCC GGGGCGGTCTTCCGCAGGAAAAGGCGCTGGCCATGCTGGTCTTGGTGGTTTCGCCGTGGT CTGCGGCCCGGGGAGTGCTTCGAAACTACTGGGAGCGACTGCTACGGAAGCTTCCGCAGA GCCGGCCGGGCTTTCCCAGTCCTCCGTGGGGACCAGCATTAGCAGTACAGGGCCCAGCCA TGTTTACAGAGCCAGCAAATGATACCAGTGGAAGTAAAGAGAATTCCAGCCTTTTGGACA GTATCTTTTGGATGGCAGCTCCCAAAAATAGACGCACCATTGAAGTTAACCGGTGTAGGA GAAGAAATCCGCAGAAGCTTATTAAAGTTAAGGTAATGCATTGATTTTTGTGGGTGTGCT TTTCCTCAAGTCCTCCATTGAATAAGCTCATGGTAGATTCCTATAGCCTAGGAATAAATT ATTCTCAGATTATATGTGTCTACTTTCCTCTGGCCTAAATGAAATACAGTATTCTCATTA TATACATAATGAGAAGGCTAGAGCCTGCTGTCCTCTAGCCTGAGTATTAGAAACACAGTC AAGGAAGGAGTGAGTTTGGAGACTGGGCAAGGAACGAGGCTGTGAGGTGCCTACATGACT TCTGAATTGTGAGCTGTGTCAGTCTTGAGCTGTGTGAGTTTTGAACTACAGATGAGTAAA CATNCTAACTATGGCAGAGCCTAGAATAAAGCTAGAAGCAGTANGTATACTGAATCTTGG GGTTTACAAGCCCNTTACATATTTGTTTAATTTTTTATCATTTTCCACANAATACCATGA AAGGAAAACCCTNAGATAAGTNATATTATTAGCTAACAACCAATTAAGTGTCTATGATGT ATCTNCAANGCCCTNATGTTTATTATATCCTCATCATC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK090551 unedited NNNNCCGTTTCAGCATGNNACCGCGGCCGCATNCTAGNGATCGGTTTTTTTTTTTTTTTT TTTCAAACCTATATCTAGGAATAAGTTGATAAATCTTTTAAATATAAAATAGAAACATTA TAACCTCTAAAAGATTTATCAACTTATTCTAGATATAGGTTTGAAAGAAAATACAAAGAA AAATACTAAACAGGATCATTAAAGAAGCTTTAAACAGTTGATAAGATTAATATACTAACA TTTGTGAGGCAGATAACAAAAGAATTTCCTTTTCTGAAAGGAAGAAAGAGGCTTGTATAT TACTTGCCTAACCATATTCTCATTTTGCTCCACTACTGGAAACTGGATTGCATGCACAAC TGAAGGTATGAAGACACCCACAGTCAGCTGGTCTTCCTTTCGTGATCAAAGTACCCAACA TAACACACTATATACCGTATGTAACAGAAAATAATTCAAGGAAGAAAATAAATGCACAGG ATAAGAACTATTCCCTCAGAATAAATGCGATCAGGGAAGTCACATAATAGATCAGTGCAA GATCTCATTCCTAAAATCTTACGTCCTCTTGAGCAAATTTATGAATTACTGTAAGTCTTA AGTAATAAAGTGTTTTTCAGTTCTACATTTAAGTTCAGCCCCAACCATCACTCAATAATA CTTTCTGGCTTAAGTTCTAAGGTTTTGCTTAACAGAATGTGGATGCTTATGAAAGTCAGC TTTCTGTTCTCGAGTTTCATCTGTATGGTGCTGCTGTTGTGGAACGCCATGAAGTAAACA GTAACTGAAATCCTAACTGTTAAAACCCTACATGAACAGATCAGAGGTAATACACACTGG TAATTTTTCTTTACTAGATCAATGAAAACC
Restriction Sites	NotI-NotI
ACCN	AK090551
Insert Size	1970 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK090551.1, BAG52183.1
RefSeq Size	2734 bp
RefSeq ORF	324 bp
Locus ID	64983
Gene Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. [provided by RefSeq, Jul 2008]

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