ANKRD20A1 (BC016022) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC016022, the custom clone sequence may differ by one or more nucleotides GGGGACTATGTTGAAGAAGAGTGGTGAGAGTGGGCTCCTCGTCTTGTTCCAGTTCTCAAAGGGAATGCTT TCACCGTTTCCCCATTCAGTATTATGTTGGCTGTGGGTTTGTATAGATGGCTTTTATTACATTAAGGGAT CATGGCACAAGTTGCAGTTTCCACCCTGCCCATGGAAGATGAGGAGTCCATGGAAGATGAGGAGTCCATT GAAGATGAGGAGTCTGTTGAAGATGATTCCGTGGAGAGCAGGATGGTGGTGACATTTCTCATATCAGCTC TCGAGTCCACGATGTTGGAATTCCTGATGAATCTGCAGTCAGCATTTTGCATGAACTGTGTGTGGATTCA TTGCCTACATTGGATGATGAAGACTTGAGTGTTGCTACTAAGGCCCTGGTGTGTGATGTTCCCCATCCTG TGTCCAAGTGTTCTCATTGTTCAGTTCCCACCTATGAGTGAGAACATGTGGTGTTTGGTTTTCTGTCCTT GCAACAGTTTGCTCAGAATGATGGTTTCCAGCTTCATCCATGTCCCTACAAAGGACATGAACTCATCATT TTTTTATGCTGCATAGTATTCCATGGTGTTGTGTCCCCGAGAAAGTGTCAGAGCCTTTACGTGGACCTTC TCATGAAAAAGGAAACAGAATAGTCAATGGAAAAGGAAAAGGGCCTCCTGCAAAACATCCTTCCTTGAAG CCTAGCACTGAAGTGGAAGATCCTGCTGTGAAAGGAGCAGTACAAAGAAAGAATGTACAGACATTGAGAG CAGAACAAACCTTACCAGTGGCTTTAGAGGAAGAGCAAGAAAGGTGTGAAAGAAGTGAAAAGAAGCAATC ACAGGTCAAAGAAGGAAATAATACAAACAAAAGTGAAAAAATACAACTATCAGAAAATGTATGTCATAGT ACATCTTCTGCTGCTGCTGACAGATTAACCAAAGAAAGAAAGATTGGGAAAACGTATCCTCAGCAAATTT CCCAAGAAACTGAAGGAAGAGCATGATAGATGCATCTTAAGACAAGAAAGTGAAGAAAAAACAAACGTTA ATATGCTGTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC016022 unedited CAACTTTTGTATACGACTACTATAGGGCGGCCGCGATTCGGCACGAGGCATGACAGGCCC TGGTGTGTGATGTTCCCCATCCTGTGTCCAAGTGTTCTCATTGTTCAGTTCCCACCTATG AGTGAGAACATGTGGTGTTTGGTTTTCTGTCCTTGCAACAGTTTGCTCAGAATGATGGTT TCCAGCTTCAACCATGTCCCTACAAAGGACATGAACTCATCATTTTTTTATGCTGCATAG TATTCCATGGTGTGTAAGTGCCACATTTTCTTAATCCAGTCTATCATTGATGGGCATATG GGTTGGTTCCAAGTCTTTGCTATTGTGAATAGTGCCACAATAAACATACATGTGCATGTG TCTTTATAGCAGCATGATTTATAATCCTTTGCATATATATCCAGTAATGGGATGGCTGGA TCAAATGGTATTTCTAATTCTTGATCCTTGAGGAATCTCCACACTGTCTTCCACAATGGT TGAACTAGTTTACAGTCCCACCAACAGTGTAAAAGTGTTCCTATTTCTCCACATCCTCTC CAGCACCTGTTGATTCCTGACTTTNTAATGATTGCCATTCTAACTGGTGTGAAATGATAT CTCATAGTAGTTTCGATTTGCATTTCTCTGATGGCCAGTGATGATGAGCATTTTTTTCAT GTGTCTGCCATACATTACTCTTTAGAATTCTGGTGACCAATTCTTTTTCTGGGTGGAAAG TTGATGGAAAGTNCCAGTTTCTCTCTCTGTTATAATAATGTTCTTTCAGGGTATGGTAGA TGACCATATTTAGCTAATTGAATGTCCTATAGTAAGAAACACTATCACAGAAGTACTTAC AAAAAACTANATGCAGCATAAATATTAATAGTATTATCAGAGTTATGAAAGACCGAGGCT CTGTATAGATCTATTNCCCATGTACTTATATACTNATGTNTTCGTTTCTTTCTGGCTAAG CTATATTAT
Restriction Sites	NotI-NotI
ACCN	BC016022
Insert Size	7000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC016022.1
RefSeq Size	1092 bp
RefSeq ORF	1092 bp
Locus ID	84210

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