KLHL17 (BC033192) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC033192, the custom clone sequence may differ by one or more nucleotides GATGGGACCTCAGACCTGGCTACCGTGGAGTCCTACGACCCCGTGACTAACACGTGGCAGCCGGAGGTGT CCATGGGCACAAGGCGAAGCTGCCTGGGTGTGGCCGCCTTGCATGGACTCCTGTACTCGGCCGGCGGCTA TGACGGGGCCTCCTGCCTGAACAGTGCTGAACGCTACGACCCCCTGACCGGAACGTGGACGTCCGTCGCT GCCATGAGCACCCGGAGGCGCTATGTGCGAGTGGCCACGCTTGATGGGAACCTGTATGCTGTGGGCGGCT ACGACAGCTCCTCACACCTGGCCACTGTGGAGAAGTGAACGTGTGGTCGCCCGTGGCGTCCATGCTGAGC CGACGCAGCTCAGCGGGCGTGGCCGTGCTGGAGGGTGCCCTGTACGTGGCAGGGGGCAACGACGGCACCA GCTGCCTCAACTCGGTAGAGAGATACAGTCCAAAGGCTGGAGCCTGGGAAAGCGTGGCGCCCATGAATAT CCGCAGGAGCACGCATGACCTGGTGGCCATGGACGGATGGTTGTACGCCGTGGGGGGTAACGACGGTAGC TCCAGCCTCAACTCCATCGAGAAGTACAACCCGAGGACCAACAAGTGGGTGGCCGCATCCTGCATGTTCA CCCGGCGCAGCAGTGTGGGTGTGGCGGTCCTGGAGCTGCTCAATTTCCCGCCGCCATCCTCCCCGACGCT GTCCGTGTCCTCCACCAGCCTCTGACCCACCTACCACCAGAGGCCTGCAGCCTCCCACATGCCTTAAGGG GACCGTGGCCCCCACCAGGGACGTCCTGCGCCATCCGTTCACGTCTCTGCATCCATTCCTTCATGTCTTT ATTTAGTTGTTTATTTATTTAGTTATTTATCTTATTTATTGAGAGGTGAGGAGTGCCACGGCTGCCCGTT TACACCTTTAGCGTCTGGTCCTCCTGCGTGTCCTCCCCTCCACTGCCTGCATGGGGGGCGCGGGGAGTGA CCAGGCGGGGGCCTCACCGCCCCAGGGCCGTTGCCTGCTCAGACCTTGCAGGCTGTGGAGCAAGAGGCCC TGGGTCTCTCCAAGCAGCTGCAGACCCCAGCTCGAATTTTGCACATGGCGGGGTCCCGGGAAGGGTGGGG AGCAGGTGTCCTTCCTGTCGTCGTCTGCCGTGTGCCATCTTTCCTGGATCTTGTAGCGGGTGCACACGCG TGCACTGGGACCACACAGCAATACGAGTCCAACTTAATAAACACATTTCTGGGGTTAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC033192 unedited NGGGTTCANATTTGTATACGACTCACTATAGGCGGCNCGCGATTCGGCACGAGGCCTCGT GCCGAATTCGGCACGAGGGCGCTATGTGCGAGTGGCCACGCTTGATGGGAACCTGTATGC TGTGGGCGGCTACGACAGCTCCTCACACCTGGCCACTGTGGAGAAGTGAACGTGTGGTCG CCCGTGGCGTCCATGCTGAGCCGACGCAGCTCAGCGGGCGTGGCCGTGCTGGAGGGTGCC CTGTACGTGGCAGGGGGCAACGACGGCACCAGCTGCCTCAACTCGGTAGAGAGATACAGT CCAAAGGCTGGAGCCTGGGAAAGCGTGGCGCCCATGAATATCCGCAGGAGCACGCATGAC CTGGTGGCCATGGACGGATGGTTGTACGCCGTGGGGGGTAACGACGGTAGCTCCAGCCTC AACTCCATCGAGAAGTACAACCCGAGGACCAACAAGTGGGTGGCCGCATCCTGCATGTTC ACCCGGCGCANCAGTGTGGGTGTGGCGGTGCTGGAGCTGCTCAATTTCCCGCCGCCATCC TCCCCGACGCTGTCCGTGTCCTCCACCAGCCTCTGACCCACCTACCACCAGAGGCCTGCA GCCTCCCACATGCCTTAAGGGGACCGTGGCCCCCACCAGGGACGTCCTGCGCCATCCGTT CACGTCTCTGCATCCATTCCTTCATGTCTNTATTTAGNTGGTTATTTATTTAGNTATTTA TCTTATTGAGAGGTGAAGAGTGCCACGGCTGCCCCGTTACACCTTTAGCGTCTGGTCCTC CTGCGTGTCCTCCCTNCACTGNCTGCATGGGGGGCGCGGGGAGTGACCANNCGGGGGCCT AACCGCCCCAGGCCGTTGCCTGCTCAAACCTTGCAGCTGTGNAGCAGAGNCCCCTGGTCT CTCAGCAG
Restriction Sites	NotI-NotI
ACCN	BC033192
Insert Size	1250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC033192.1
RefSeq Size	1285 bp
RefSeq ORF	1285 bp
Locus ID	339451
Domains	Kelch
Gene Summary	The protein encoded by this gene is expressed in neurons of most regions of the brain. It contains an N-terminal BTB domain, which mediates dimerization of the protein, and a C-terminal Kelch domain, which mediates binding to F-actin. This protein may play a key role in the regulation of actin-based neuronal function. [provided by RefSeq, Aug 2010]

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