PACS2 (BC028418) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PACS-2; PACS1L
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC028418, the custom clone sequence may differ by one or more nucleotides AGCGGAGGAGTATGAGAGCTTCTCCTCCGAGCAGGAGGCAAGTGACGACGCCGTGCAGGGGCAGGACTTG GACGAGGACGACTTTGACGTGGGGAAGCCGAAGAAGCAGCGGAGATCGATTGTAAGAACGACGTCCATGA CCAGGCAACAGAACTTCAAGCAGAAAGTGGTAGCGCTGCTGCGGAGGTTCAAAGTGTCCGACGAGGTCCT GGACTCGGAGCAGGACCCTGCGGAGCACATCCCCGAGGCAGAGGAGGACCTGGACCTCCTGTATGACACC CTGGACATGGAGCACCCCAGCGACAGCGGCCCCGACATGGAGGATGACGACAGCGTCCTCAGCACCCCCA AGCCGAAGCTGCGGCCATACTTTGAAGGCCTGTCGCACTCGAGCTCGCAGACGGAGATTGGGAGCATCCA CAGCGCCCGCAGCCACAAGGAGCCCAAGCCCGGCTGACGTGCCCGAGAAGACGCGGTCCCTGGGAGGCAG GCAGCCGAGCGACAGTGTCTCTGACACGGTGGCCCTCGGTGTGCCAGGCCCGAGGGAGCACCCTGGACAG CCTGAGGACAGCCCCGAGGCTGAGGCCTCCACCCTGGATGTGTTCACGGAGAGGCTGCCGCCCAGCGGGA GGATCACCAAGACAGAGTCCCTTGTCATCCCCTCCACCAGGTCCGAGGGGAAGCAGGCTGGCCGACGGGG CCGGAGCACATCCTTGAAGGAGCGGCAGGCAGCACGGCCCCAGAATGAACGGGCCAACAGCCTGGACAAC GAGCGCTGCCCGGACGCCCGGAGCCAGCTACAGATCCCCAGGAAGACTGTGTATGACCAGCTCAACCACA TCCTCATCTCCGATGACCAGCTTCCCGAAAACATCATCCTTGTCAACACCTCGGACTGGCAGGGGCAGTT CCTCTCCGACGTCCTGCAGAGGCACACGCTCCCCGTGGTGTGCACGTGCTCTCCTGCGGACGTCCAGGCG GCCTTCAGCACCATCGTCTCACGGATACAGAGATACTGCAACTGCAATTCCCAGCCCCCGACCCCCGTGA AGATCGCCGTGGCGGGAGCGCAGCATTACCTCAGTGCCATCCTGCGGCTCTTTGTGGAGCAGCTGTCCCA CAAGACACCCGACTGGCTCGGCTACATGCGCTTCCTGGTCATCCCACTGGGTGAGCACCACGCCGTCCAC CTGGGCCTGGGCACAGATGCCACGGGCAGTGTGGCGTGGCATGGAGTGGTGTGGCGTGGCGTGGCGTGTT GTGTGGCGTGGCGTGGCGCGGTGTGTCGTGGTGTGGCGCGGTGTGGCATGGCGTGGTGTCCCTGCTTGCT ACACATTTGTTGTTTTTAAATGTTTGTTTGATACAGTAACACTTTGTTAATTTTAATTATATGCAAGATA ACTTGATTGCCCTAAAACAGCCATTTGGGTCAAGATAAAGCCATCGCCCTCTGAAGGGGCCTGAGCTGGG TGTCTCCTCCATCAGTCGCTGTGATAACTCTATGCCAGTTTCGATTTCCAAAGTCAGAAGTGCAAAGCAG GGCTCGTTATTAATCCTCTCAAATCGTCCAGGTCCTGTGTCGTCGAACTCCATTACTTCTTTAGGTCCAT ACGATGTGTCTTACCACGAAACAAACCCACCCAGCCTTAATCTGTTGACCAGCAGCAAGGCCTGGAGGCG ACACCTCTCCCGTGTGGCCCTCACGATGCCGACGTCAGAGCCCTGAGCCAGCCGAGGCCTCTGCACGGTC ACATGTGCCCTGCTGGGCCTTCCTCAGTGGCACTCAGGGCTCTGGGACCTCAGCTCAGGGCTGGAGCTCC TTCAGCGGGGCCACAGGCATCCACCAGGGAGGGGCAGTGGTGGCTTTCTCTTTCGGTGGCAGGAAAGTGG GCTTGTCTGGAAGGCAGCAGCACAGGCCTGGGGTCGGGTGGCCCAGCTTTTGTGCCGCGGTGGGAGCCTT GCAGCTCCGAGTCCCCCGTGGTGACACCAGCCCCACCCCTGGCATGCAGGCTCCCACCCCGTGGCCAGGT ACCTAGGCTCCGTGGACTACCGCTACAACAACTTCTTCCAGGACCTGGCCTGGAGAGACCTGTTCAACAA GCTGGAGGCCCAGAGTGCGGTACAGGACACGCCAGACATTGTGTCACGCATCACGCAGTACATCGCAGGG GCCAACTGTGCCCACCAGCTCCCCATCGCAGAGGCCATGCTGACCTACAAGCAGAAGAGCCCTGACGAAG AGTCCTCCCAAAAGTTCATTCCCTTTGTCGGGGTTGTGAAGGTTGGAATTGTGGAGCCATCCTCGGCCAC ATCAGGCGACTCGGACGACGCGGCCCCCTCGGGCTCTGGCACGCTCTCCTCCACCCCGCCGTCCGCATCT CCTGCGGCCAAGGAGGCCTCACCCACCCCGCCCTCCTCCCCGTCGGTGAGCGGAGGCCTGTCCTCCCCCA GCCAGGGTGTCGGCGCCGAGCTGATGGGGCTGCAGGTGGACTACTGGACGGCAGCACAGCCTGCGGACAG GAAGAGGGACGCCGAGAAGAAGGACCTGCCTGTCACCAAAAACACGCTCAAGTGCACTTTCCGGTCCCTC CAGGTCAGCAGGCTGCCCAGCAGCGGCGAGGCTGCAGCCACGCCCACCATGTCCATGACCGTGGTCACCA AGGAGAAGAACAAGAAGGTGATGTTTCTGCCCAAGAAAGCGAAGGACAAGGACGTGGAGTCTAAGAGCCA GTGCATTGAGGGCATCAGCCGGCTCATCTGCACTGCCAGGCAGCAGCAGAACATGCTGCGGGTCCTCATC GACGGCGTGGAGTGCAGCGACGTCAAGTTCTTCCAGCTGGCCGCGCAGTGGTCCTCGCACGTGAAGCACT TCCCCATCTGCATCTTCGGACACTCCAAGGCCACCTTCTAGCCCCACCCACCAGGGGGCCCACCTCCTGC CCCATGCTGTGAGGGGCCCAGCTGCATTTCTGTTAACATTTCAGTTTACTACAGAGACAGACGCTTAAAA CACAAAGAGAAACAGTCTTAAGTATGAATGTGCTCACAACGTGGAAACTAACGGGGGAGCTCCTGCCAGG AGCCGAATAACTGCTCTGCTTATTAACCCGAACGTTCGGCCCGGGGCTGGGAAGCCAGAAGGACGATGCT GAGCCATGGATCGCGGAAGGCGTCCTCTGGCCTCAGGAGCCACCCAGAGCCTCACAGGCTGAGTTCTTGC CTCTGTGTCCTGTCCTTCCTGGAAGTCAGGACTCTGCTTCCTCAGGGAGCCCGGGGAAGGCGGAGCTCAG TGGCCACAGGCCGAGGGCCATGGGGCCGCTCAGTCCCGTTGGGGTTGTCCTGAGTTGAGCCTGGGGGGGC CGTCCTGCCCGCCTAAGAGATGCCCCCAGCACCGCACACTCGTGGTTCCCAATAAACTCCTGCCTGCGGC GGAGGTTTTATAGCAGCAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC028418 unedited CGCGTAATTCGCACGAGGGCCAGGTGCTGAGCCTCTGCAGCAGCATCAAGGAGGCCCCCG TCAAGGCGGCCGAGATCTGGATCGCCTCCCTGTCCAGCCAGCCCATTGACCACGAAGACA GCACCATGCAGGCCGGCCCCAAGGCCAAGTCCACGGATAACTACTCCGAGGAGGAGTATG AGAGCTTCTCCTCCGAGCAGGAGGCCAGTGACGACGCCGTGCAGGGGCAGGACTTGGACG AGGACGACTTTGACGTGGGGAAGCCGAAGAAGCAGCGGAGATCGATTGTAAGAACGACGT CCATGACCAGGCAACAGAACTTCAAGCAGAAAGTGGTAGCGCTGCTGCGGAGGTTCAAAG TGTCCGACGAGGTCCTGGACTCGGAGCAGGACCCTGCGGAGCACATCCCCGAGGCAGAGG AGGACCTGGACCTCCTGTATGACACCCTGGACATGGAGCACCCCAGCGACAGCGGCCCCG ACATGGAGGATGACGACAGCGTCCTCAGCACCCCCAAGCCGAAGCTGCGGCCATACTTTG AAGGCCTGTCGCACTCGAGCTCGCAGACGGAGATTGGGAGCATCCACAGCGCCCGCAGCC ACAAGGAGCCCCCAAGCCCGGCTGACGTGCCCGAGAAGACGCGGTCCCTGGGAGGCAGGC AGCCGAGCGACAGTGTCTCTGACACGGTGGCCCCTCGTGTGNCCAGCCCGAGGNAGCACC CTGNACAGCCTGAGGACAGCCCCGAGGCTGAGGCCTNCACCNNCTGGATGTGTCACGGGA GAGGGCTGCCGCCCACGGGNAGGATCACCAGNACANATNCCCTTGTCATCCCCTNCACCC AGTCCGAGGGGAAACANGCCTGCCNGACG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC028418 unedited NCCGTATTACTATGNACGCGGCCGCATTCTAGNGATCGGTTTTTTTTTTTTTTTTTTTTG CTGCTATAAAACCTCCGCCGCAGGCAGGAGTTTATTGGGAACCACGAGTGTGCGGTGCTG GGGGCATCTCTTAGGCGGGCAGGACGGCCCCCCCAGGCTCAACTCAGGACAACCCCAACG GGACTGAGCGGCCCCATGGCCCTCGGCCTGTGGCCACTGAGCTCCGCCTTCCCCGGGCTC CCTGAGGAAGCAGAGTCCTGACTTCCAGGAAGGACAGGACACAGAGGCAAGAACTCAGCC TGTGAGGCTCTGGGTGGCTCCTGAGGCCAGAGGACGCCTTCCGCGATCCATGGCTCAACA TCGTCCTTCTGGCTTCCCAGCCCCGGGCCGAACGTTCGGGTTAATAAGCAGAGCAGTTAT TCGGCTCCTGGCAGGAGCTCCCCCGTTAGTTTCCACGTTGTGAGCACATTCATACTTAAG ACTGGTTCTCTTTGTGTTTTAAGCGTCTGTCTCTGTAGTAAACTGAAATGTTAACAGAAA TGCAGCTGGGCCCCTCACAGCATGGGGCAAGAGGTGGGCCCCCTGGTGGGTGGGGCTAGA AGGTGGCCTTGGAGTGTCCGAAGATGCAGATGGGGAAGTGCTTCACGTGCGAAGACCACT GCGCGGCCAGCTGGAAGAACTTGACGTCGCTGCACTCCACGCCGTCGATGAGGACCCGCA GCATGTTCTGCTGCTGCCTGGCAGTGCAAATGAACCCGCTGATGCCCTCAATGCACTGGC TCTTAAACTCACGTCCCTGGCCTTCCCTTTTTGGCAGAACATCCCTTCTTGGTCTTCTTC TTGGTGACACCGTCAATGACCTGTGGGGCGTGGCTGCCACTCACA
Restriction Sites	NotI-NotI
ACCN	BC028418
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC028418.1
RefSeq Size	3463 bp
RefSeq ORF	3463 bp
Locus ID	23241
Gene Summary	Multifunctional sorting protein that controls the endoplasmic reticulum (ER)-mitochondria communication, including the apposition of mitochondria with the ER and ER homeostasis. In addition, in response to apoptotic inducer, translocates BIB to mitochondria, which initiates a sequence of events including the formation of mitochondrial truncated BID, the release of cytochrome c, the activation of caspase-3 thereby causing cell death. May also be involved in ion channel trafficking, directing acidic cluster-containing ion channels to distinct subcellular compartments.[UniProtKB/Swiss-Prot Function]

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