ARHGEF10L (BC029928) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GrinchGEF
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC029928, the custom clone sequence may differ by one or more nucleotides CGCCATTGTATGGAGAGACCACATTTTGTTTACGCGTTAGTCTGTCGACGGACATTTTTGCCTCCACATT TTGGCGACTGTGGCTAATGCTGCTATGAACATGGCTGGACAAATATCTGTTTGAGACCCCGCTTTCAGTT CATGTGGGTCTATTCCTAGAAAGATCCATGCTTTTGTTTTCTTTTTCATGCATTAGCTGTTTCTTGAGGT CTTGTTCTGCATCTGGTGCCATCTGTCCTGGCCTCAGGCCGGGCCCTTTGCAGACCCAGTGTGTGTAGGG GGTTGCGCAGGGTGAGGCTTTGCCCCAGGCTGGGGTAGAGTTGGTCATGGGTGGGCTGCTCAGTCTCTGC TCTGACCTGGCAGGACATGCTGAAGAACACCCCCAGGGGCCATCCGGACAGGCTGTCGCTGCAGCTGGCC CTCACAGAGCTGGAGACGCTGGCTGAGAAGCTGAACGAGCAGAAGCGGCTGGCTGACCAGGTGGCTGAGA TCCAGCAGCTGACCAAGAGCGTCAGTGACCGCAGCAGCCTCAACAAGGTGAGCTGGGCCTCCCACCTGCC TGCCCTCACCTGCCTGCCCTCACCTGTGCTCCTGCCCGTGCCCCTGCCCCACGCCACCCACACTAGGTGG CAGTGTTCTCTGAGGGAGGGGAGGCCTTGCTCTTCCAAGGATGTGACCTTGGGGATTGGATCCTCAGCGG GACCCCCACGTTCATTCCTGTTCATGTTATTTTAAGATAAAAGTTCATTTTGTGCATCAGTGTGATTTCC AGTAAAGTCTCCAGAAATCAAAGCCTAGTTAAATGAACAGGCTCTGGAGAGTCAGAATCTGGGTTTAGAT CCAGATTTTGCCATAGGTGCTTGCTGTGTGCCCTCAAGGGAATCACCCACCCTCTCTGAACCTCACTTGC TCCCTTTGAAATGAGGAGCATTTAATAGCACCGGAGCCCTAGGGCTGCTGCCGCAAAAGAGGGAGCTGGC AAAGTGGCTTCGTGATTGTCCTTAACACAAGAAGAAAGATGAAAAGTCCATGCCATGTTTTGGGGTTGCC GCTTAGGAACATGCTATTAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC029928 unedited NGGGGGGTGTGCACTATTTTGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACG AGGGCTACGTGGAGTCTCTGAAGCGGATACTCCAGGACTACCGCAACCCCCTGATGGAGA TGGAGCCCAAGGCGCTGAGCGCCCGCAAGTGCCAGGTGGTGTTCTTCCGCGTGAAGGAGA TCCTGCACTGCCACTCCATGTTCCAGATCGCCCTGTCCTCCCGCGTGGCTGAGTGGGATT CCACCGAGAAGATCGGGGACCTCTTCGTGGCCTCGTTTTCCAAGTCCATGGTGCTAGATG TGTACAGTGACTACGTGAACAACTTCACCAGTGCCATGTCCATCATCAAGAAGGCCTGCC TCACCAAGCCTGCCTTCCTCGAGTTCCTCAAGCGACGGCAGGTGTGCAGCCCAGACCGTG TCACCCTCTACGGGCTGATGGTCAAGCCCATCCAGAGGTTCCCACAGTTCATACTCCTGC TTCAGGACATGCTGAAGAACACCCCCAGGGGCCATCCGGACAGGCTGTCGCTGCAGCTGG CCCTCACAGAGCTGGAGACGCTGGCTGAGAAGCTGAACGAGCAGAAGCGGCTGGCTGACC AGGTGGCTGAGATCCAGCAGCTGACCAAGAGCGTCAGTGACCGCAGCAGCCTCAACAAGC TGTTGACCTCAGGCCAGCGGCAGCTGCTCCTGTGTGAGACGTTGACGGAGACCGTGTACG GTGACCGCGGGCAGCTAATTAAGTCCAAGGAGCGTCGGGTCTTCCTGCTCAACGACATGC TTGTCTGTGCCAACATCAACTTCAAGGGCCAGCTGGAGATCAGCAGCCTGGTGCCCCTGG NGCCCAAAGTATGTGTGAAGTGGAACACGGCGCTGCCCCAGTGCCAGTGGTGGAGTGGGC CAGC
Restriction Sites	NotI-NotI
ACCN	BC029928
Insert Size	3500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC029928.1
RefSeq Size	1088 bp
RefSeq ORF	1088 bp
Locus ID	55160
Gene Summary	This gene belongs to the RhoGEF subfamily of RhoGTPases. Members of this subfamily are activated by specific guanine nucleotide exchange factors (GEFs) and are involved in signal transduction. The encoded protein shows cytosolic distribution. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2016]

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