AK001460 Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK001460, the custom clone sequence may differ by one or more nucleotides ACTTTTCCAGATCTAGGAGCACCATGGACACCTCGTCAGAGATGCTGGTACGGTTTGGACGGCGCTGTGG ACGGGCGAAGGAAAGTACAGTCTTAGGTATGTCTTTATCAGCAGTGTGAAAACGGACTAATACAGTAAAT TGGTACCAGCAGAGTGGGGCATTGCTGAAAAGATAACTGAAAATGTGGAAGCGACTTTGGCACTGGGTAA CAGGCAGAGATTGGAACAGTTTGAAGAGCTCAGAAGAAGACAGGAAAATGTGGGAAAGTTTGGAACTTCC TAGAGACTTGTTGAATGCCTTTGACCAAAATGCTGATAGCGACATGGACAATAAAATGCAGGCTGAGATG GTCTCAGATGGAGATGAGGAACTTTCTGGAAACTGGAGTAAAGGTGATTCTTGCTATGTTTTAGCAAAAA GACTAGCGTCATTTTACCTCTGCCCTAGAGATTTGTGGAACTTTGAGAAAGATGATTTAGGGTACCTCGC AGAAGAAATTTCTAAGCAGCAAAGCATTCAAGAGGTGAGTTGGATACTGTTAAGGGCAGTCAGTTTTAAA AGGGAAACAGAGCATAAAAATTCAGAAAATTTGTAGCCTGATCTGGCAGAAGAAATTTCTAAGCAGCAAA GCATTCAAGAGGTGAGTTGGGTACTGTTAAGAGCATTCAGTTTCAAAAGGGAAACAGAGCATAAAAATTC AGAAAATTTGTAGCCTGACTATGCAATAGAAAAGAAAAACCAGTTTTCTGGGGAGAAATTCAAGCCAGCT GCAGAAATCTGCATAAGCATCAAGAAGCCTAATGTTAATCCCCAGGACCATGGGGAAAATGTCTCCAGGC CATGTCAGAGACCTTCACAGCAGCCCCTCCTGTCACAGGCCCAGAGATCCAGGAGGAAAAAATGGTTTTA TGGGCCAGGACCAGGGTCCCTGTGCTGTGTGCAGCCTATAGACTTGGTGCCCTGTGTCCCAGCTGCTCCA GCCATGGCTGAAAGGGGCCAATGTAGAGCTCATGCTGTGGCTTCAGAGGGTGGAAGCCCCAAGCCTTGGC AGCTTCCACATGGTGTTGAGCCTGTAGGTACACAGAAGTCAAGAATTGAGGTTTGGGAACCTCCACCTAG ATTTCAGAAGATGTATGGAAATGCCTGGATGTCCAGGCAAAAGTTTGCTGCAGTGGCAGGGCCCTCATGG AGAACCTCTGCTAGGGCAATGCAGAAGGGAATTGTGGGGCCGGAGCTCCCACGTAGAGTCCCTAATGGGG CATTATCTAGTGGAGCTGTGAGAAGAGGGCCACCGTCCTTCAGACCCCAGCATGGTGGATCCACTGACAG CTTGCACCATGCGCCTGGAAAAGCCGCAGACACTCAATGCCAGTCCGTGAAAGCAGCCAGGAGGGAGGCT GTACCCTGCAAGGCCACAGGGGCAGAACTGCCAAAGGCCATGGGTCCCCACCTTTTGCATCAGCGTGACC TGAATGTGAGACCTAGAGTCAAAGGAGATCATTTTGGAGCTTTAAAATTTGATTGTCCTGCTGGATTTTG GACTTGCATGGGTCCTGCAGCCCCTTTGTTTTGGCCAATGTCTCCCATTTGGAATGGCTGTATTTACCCA ATACCTGTACCCCTATCGTATCTAGGAAGTAACTAGCTTGCTTTTCATTTTACAGGCTCATAGGCAGAAG GGACTTGCCTTGTCTCAAATGAGACTTTGGACTATGGACTTTTGGGTTAATGCTGAAATGAGTTAAGACT TTGGGGGACTGTTGTGAAGGCATGATTGGTTTTGAAATGTGAGGACATGAGATTTAGAGGGGGCCAGGGG CAGAATGATATGGTTGGGCTGTGTCCCCACCCAAATCTCAACTTGAATTGTATCTCCCAGAATTCCTACA CGTTGTGGGAGGGACCCAGGGAGAGGTAATTGAATCATGGGGGCTGGTCTTTACCATGCCATTCTTATAA TAGTGAATACGTCTCATGAGATCTGATGGGATTATCAGGGACTTCCGCTTTTGCTTCTTCCTCGTTTTCT CTTGCTGCCACCATGTAAGAAGTGCCTTTCGCCTCTCACCATGATTCTGAGGCCTCCCCAGCCATGTGGA ACTGTAAGTCCAACTAAACCTTTTTTTCTCTCCAGTCTCAGGTATGTCTTTATCAGCAGCATGAAAATAT ACTAATACATATTTCATGTTAATGGGCTGGGAGATTTAGTATTGTTAAGATGGTTTGAATATTTGTCCCC TTCAAAACTCATGTTGAAAATTAATTCCCAAAATGACAGTATTGAGAGGTGGGGCCTTTAAGAAGTGATT GGGTCATGAGGGATCTGCTTTCATGAATGGATTAGAAATGGGGTCTTGCTGTGAATGGATTAATGGCTTA TGCTGGAATTGAGACTGTTGGCTTCATAAGAAGAGGAAGAGAGACCTGAGCTAGCATCCTCAGCCCCCTT GCCATATGATGCCCTGCATTACTTCCAGACTCTGCAGAGAGACCTTACCAGCAAGAAAGCCCTCACCAAA TGCAGCCCCTCAACCTTGCACTTCTGAGCCTCTATAATTCTAAGAAATAAAATCCTGTTCTTTATAAATT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK001460 unedited CAACATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGGCATCATGGACA CCTCGTCAGAGATGCTGGTACGGTTTGGACGGCGCTGTGGACGGGCGAAGGAAAGTACAG TCTTAGGTATGTCTTTATCAGCAGTGTGAAAACGGACTAATACAGTAAATTGGTACCAGC AGAGTGGGGCATTGCTGAAAAGATAACTGAAAATGTGGAAGTGACTTTGGCACTGGGTAA CAGGCAGAGATTGGAACAGTTTGAAGAGCTCAGAAGAAGACAGGAAAATGTGGGAAAGTT TGGAACTTCCTAGAGACTTGTTGAATGCCTTTGACCAAAATGCTGATAGCGACATGGACA ATAAAATGCAGGCTGAGATGGTCTCAGATGGAGATGAGGAACTTTCTGGAAACTGGAGTA AAGGTGATTCTTGCTATGTTTTAGCAAAAAGACTAGCGTCATTTTACCTCTGCCCTAGAG ATTTGTGGAACTTTGAGAAAGATGATTTAGGGTACCTCGCAGAAGAAATTTCTAAGCAGC AAAGCATTCAAGAGGTGAGTTGGATACTGTTAAGGGCATTCAGTTNTAAAAGGGAAACAG AGCATAAAAATTCAGAAAATTTGTAGCCTGATCTGGCAGAAGAAATTTCTAAGCAGCAAA GCATTCAAGAGGTGAGTTGGGTACTGTTAAGAGCATTCAGTTTCAAAAGGGAAACAGAGC ATANAAATTCAGAAAATTTGTAGCCTGACTATGNCATAGAAAAAGAAAACCAGNTNTCTG GNNGAGAAAATCAGCCAGCTGCAGAAATCTGCATAAGCATCAAGAAGCCTAATGTTAATC CCCAGGACCATGGNGNAAATGTCTNNCAGCCATGTCAGAGACCTTCACAGCAGCCCCTTC TGTCACAGGNCCAGAGATCCCAGAGGAAAAATGGNTNTATGGGCCANGACCANNGTNCCT GTGCTGTGTGCAGCTATAGACTTGTG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK001460 unedited CTATGGAACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAAAAACAGGG TCTCTCTTGTAGCCCTGGCTGGAGTGCAGTGGCTATTCATAAGCACAACCATTGTGCACT ACACCCTCGAACTCCCGGTCTCAAGTGATCCTTCTGGCCACAACCACCTGAGTAGCTGGG ACTATAGGTGCACATTCCCATGTCCAGCCAATGAGTGTAGCTTTGTAGTAAGTCTTAATA TCATGAAGCGTGAGTCCTCCTACTTATTAATATTTCTTCAGATTCAAAATTGTTTTAGCT ATTCTATTGTCCTTTTCATTTCCATATGAATTCTAAGATCAGCTTGTCAATATCAACAAA AAAGCCAAGTAGAATTTTGATAGGAATTGTGTTGAATGTGTAGATCAATTTGGGAAGTAT TGCCATCTGTCTTAGTCCATTTTCTGTTGCTATAACAGAATACCTGAAACTAGATAATTT ATAAAGAACAGGATTTTATTTCTTAGAATTATAGAGGCTCAGAAGTGCAAGGTTGAGGGG CTGCATTTGGTGAGGGCTTTCTTGCTGGTAAGGTCTCTCTGCAGAGTCTGGAAGTAATGC AGGGCATCATATGGCAAAGGGGCTGAGGATGCTAGCTCAGATCTCTCTTCCTCTTCTTAT GAAGCCAACAGTCTCAATTCCAGCATAAGCCATTAATCCATTCACAGCAAGACCCCATTT CTAATCCATTCATGAAAGCAGATCCCTCATGACCCAATCACTTCTTAAAGCCCCACCTCT CATACTGTCATTTTGGGAAATAATTTTCACATGAGTTTTTGAAGGGACCAATATTCAAAC ATCTTAACATACTAAATCTCCAGCCCATTACATGAAAATGTATTAGTTATTTTCATGCTG CTGAAAAAAAATACCTGAAACTGGGGAGAAAAAAAGTTTATTTGGACTTCAGNTCCCATG GCTGGGGAGGCTCCAAATCTGGTGGG
Restriction Sites	NotI-NotI
ACCN	AK001460
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK001460.1
RefSeq Size	2590 bp
RefSeq ORF	2590 bp
Locus ID	401132
Gene Summary	DISCONTINUED: This record has been withdrawn by NCBI because the model on which it was based was not predicted in a later annotation.

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