ZNF229 (AK091541) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC103301 sequence for AK091541 edited (data generated by NextGen Sequencing) ATGGCTCTAATCTTCGCACCCACCAGAGGTTGCACACAGGAGAGAAACCCTACACTTGTT GTGAATGTGGGAAGGGTTTCAGATATGGCTCAGGTCTCCTTAGTCATAAGAGAGTGCACA CTGGCGAGAAGCCATACAGATGCCACGTGTGTGGGAAGGGCTATAGTCAGAGCTCACATC TTCAAGGTCATCAGAGGGTCCACACTGGTGAGAAACCCTATAAATGTGAGGAGTGTGGGA AGGGCTTTGGCCGCAACTCCTGTCTTCATGTTCATCAGAGAGTCCACACTGGAGAGAAGC CCTATACGTGTGGTGTGTGTGGGAAAGGCTTCAGTTATACCTCAGGTCTGCGGAACCACC AAAGAGTGCATTTAG Clone variation with respect to AK091541.1 278 a=>g 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK091541 unedited GGGCCGCGATTCGCCGAGGGAAAACCTTACAGAGCCAAGATGCAGAAGGGCTTTAGGTGC ACATCAAGCCTTCACAAACATCAGCGAGTCCACACGGGNAAAAAAGCCCTATACGTGTGA TCAGTGTGGCAAGGGATTCAGTTATGGCTCTAATCTTCGCACCCACCAGAGGTTGCACAC AGGAGAGAAACCCTACACTTGTTGTGAATGTGGGAAGGGTTTCAGATATGGCTCAGGTCT CCTTAGTCATAAGAGAGTGCACACTGGCGAGAAGCCATACAGATGCCACGTGTGTGGGAA GGGCTATAGTCAGAGCTCACATCTTCAAGGTCATCAGAGGGTCCACACTGGTGAGAAACC CTATAAATGTGAGGAGTGTGGGAAGGGCTTTGGCCGCAACTCCTGTCTTCATGTTCATCA GAGAGTCCACACTGGAGAGAAGCCCTATACGTGTGGTGTGTGTGGGAAAGGCTTCAGTTA TACCTCANGTCTGCGGAACCACCAAAGAGTGCATTTAGGCGAGAACCCTTATAAGTAGAT GTACATAGAGGATTCCATCTGGGACTCAGAGCTTTCTATCCATCTGAGAGCCAACACAGG AGAAAAACCTAGGAAAGTGACATGTAGGAGGGCTATAGGAGAAACTGATGATTTTACATT TTCTAGTCTGCATAGGAAGGGAACACCTGGACGTGTGATAAATAGGTTAGCACCTTCAGT TACAAACTTCAGTTTTTAAAAGTNCTGTGTGGCAACGAAGTAGGCTTTAAAGAACAGGGC AACANTGGGTTTCATAATAATTCTCACTTCCTCAAAACTGCCTGGGAGGGAGCTTTTACA TGTACAAGTGGGGGA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK091541 unedited CTATGGACCGCGGCCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGGGGTTCCCCCCGGAATCTTTAAT CATAGGGGGTTGTTCCCCCCGGCTAATTTATAAAAAATACCCCTCCGGTTTTTAAAAAAC GGAAAAATCCTTTGTTCCCAAGTAAAATCCCTAAAAGTCCCCCCTTTGTTTTATCCCTTC AAAACCCCCCCCCTAGAAAAAAGTTTTTTTTTGCCCCCCCCCCCATCTTTTTTTCCCGGG GGCCCTAAACCTCCCCCCCGAAAAACCTCCTACCAAAAGGGGGGCCGGCCATAATTGTTG TTCTAAAACCCCCCGGGTTCACAAAAAACTTCAGGAGCAACCCGCCCTGGCTTTTTCTGG GGGCCCGAAAATTTCTAGGGGCTTTCCCTTGGAAACCTTTGCCGGAACTTTTTTAAAAAA TTTCCCCCCCAGGGAAACGGGGAAATTAACCTTTGCTTTTTTAAAAAGGTTTTAAATTTA AAAACCCCCCCTTTTGCAGGCGTTTTTGCCCAAGTGGGCAACTTTCTCCCAAGTTTCAAT TACCCCATTTTTCCCGCCCCTTCAAAGGTTATAAAAATTGAATTGATCCCTTAAGGAATT CTTTTGAAAACAATGCCCGGGTTTTCTCTTCTCCCCATAAGTCCCTAAGACTTTTTCCGT CCCTTTTTGGCCCGCTTTAAGAACCACCTGAAACTTGTAATTTGCAAAGGGAAAGAGTTT ACCTCCCCCGTTCCCCATGGGTGGGGGGCCTCCCAAACTTTTTCCTGGGAAACGCACAAG AAAATAAGGCCTTTTCCCCGGGGACCGGAAGAACCCCGGGAGGGCCTCCCTTAAAACTCC GCCCTCTTGGAACCTCCTTTCTGGGAACCCCCGGGGAGATTTTCCCTCGTGTTCAATATT TCCCACAGGCCCCCTTGAACAG
Restriction Sites	NotI-NotI
ACCN	AK091541
Insert Size	2650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK091541.1, BAC03689.1
RefSeq Size	2523 bp
RefSeq ORF	2523 bp
Locus ID	284350

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