STAG3L1 (BC030509) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DKFZP434A0131
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC030509, the custom clone sequence may differ by one or more nucleotides ATGGGTGGAAGAGAGCAACGCCAGAGCCCAGGTGCCCAGAGGACTTTCTTCCAGCTTCTGCTGTCCTTCT TTGTGGAGAGCAAGCTCCACGACCACGCTGCTTACTTAGTAGACAACCTGTGGGACTGTGCAGGGACTCA GCTGAAGGACTGGGAGGGTCTGACAAGCCTGCTGCTGGAGAAGGACCAGAGCACGTGCCACATGGAGCCA GGGCCAGGGACCTTCCACCTCCTAGGGTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC030509 unedited GATTTTGTAATACGACCTCACTATAGGGCGGCCGCGAATTCGCACGAGGGTCGCCCTCCG TCGTGGTCTGGCGTGTATTCCGAGCGTTGGTGTCTGGCGGTTTCCGAGCGTTGGTGTCTG GCGGTTTCCGACCGTTGGTGTCTGGCGGTTTCCGACCGTTGGTGTCTGGCACGCGCCACC CTCTCTTGCTTTGGTTGCGCCATGCCGATGTACCAGGTAAAGCCCTATCACGGGGTCTGC GCCCCTCTCCGTGTGGAGCCCACCTGCATGTACTGGCTCCCCAACATGCACGGCAGGAGC GGCGGCCCAGCACTTGGCACTGGCCACTTGCAGACAAGAAGACAAGAAAATGATTTGAGG ACAGCTTCAATCGCGGTGTGAAGAAGAAAGCAGCAAAACGACCACTGAAAACAACGCCGG TGGCAAAATATCCAAAGAAAGGGTCCCAAGCGGTACATCGTCATAGCCGGAAACAGTCAG AGCCACCAGCCAATGATATTTTCAATGCTGCGAAAGCTGCCAAAAGTGACATGCAGGGAT GTCCTTCCTGAGATCCGTGCTATCTGCATTGAGGAAATTGGGTGTTGGATGCAAAGCTAC AGCACGTCTTTCCTCACCGACAGCTATTTAAAATATATTGGTTGGACTCTGCATGATAAG CACCGAGAAGTCCGCGTGAAGTGCGTGAAGGCTCTGAAAGGGCTGTACGGTAACCGGGAC CTGACCGCACGCCTGGAGCTCTTCACTGGCCGCTTCAGGACTGGATGGTTTTCCATGATC GTGACAGAGAGTACAGTGTGGCAGTGGNAGCCGTCAGATACTGATACTATCCTTAGTGAG TCCTGGGAAGAGGGGAGCCAGTGTCTCAAACCTTGCCCTTCAGGTCATCTCCTTCACTGT ACAGTGAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC030509 unedited NGGGTATTACTATGNACCGCGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTGGT AGAAGACAGGGTCTCACTATGTTGCCCAAGCTGGTCTCAAACTCCTGGGCTCAAAGGATC CTCCCACCTCAGCCTCCCAAAGTGCTGGGCTTACAGGCGTGAGCCACCAACCCCAGCCCA CTTGTAGGTCCTATGCACAACAGCCAGAGTTCTCTCTGTGTATGTCACCTTGCTCTCCAC AAAGAAGGACAGCAGAAGCTGGAAGAAAGTCCTCTGGGCGCCTGGGCTCTGGCGTTGCTC TCTTCCACCCATCGTTCTTATCTCGCACTCAGGGTAGAAAAGTCTGGTGGAATGGACACA AGGAAAGGGCTCAGCACAGGAGGAAACAAGGAGGTGTAATCATGACCACAGACGGAGGAG ATGGCCCAAGGAGATGATAAAGGAAAAGGAGGTGCAGAGCTAAGGAAAGCCCCAAGGAGC TGAGAGAGGAAAAGAGACACCAGAGAGACTTCCACGGAGATGGGGAGGCCTTAAGTAGGG CAGCAGCACATGTGGGAGAGAAACCGAGAGGGTGGTGTTAAAGAAACGTAAAAGGAGCCC CACTCACTTCCAGTACAGAAATTCACCCACAGCAGAGGCCAGGCCTCAATTAGAGGCCTA CACAATGGGGTAGACGCTCTCACAGTCCACGTCCATCAACACCCCTTCCATGTTCCTGCC CACAGAAAAGCAGAAAGAGTGTGTGAATAGGGACATTGTCTTCTGGCACAATCAAAGCTA GAAAGAAAAAGAGGCAAAAGCAACACGGACATAACTCACAAAAAACTCCTAAGTGGAAAT GGAAGACAAGGGATATGGGGAGTTAGAGAAGACAAAATATACCCTCTCAAATACTGGACT TAGG
Restriction Sites	NotI-NotI
ACCN	BC030509
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC030509.1, AAH30509.1
RefSeq Size	1286 bp
RefSeq ORF	240 bp
Locus ID	54441

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