VRK3 (AK074461) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK074461, the custom clone sequence may differ by one or more nucleotides CACTCTGCCTAGGACGCACCTAATTGCCCAGCCACAGGCTATGACAGTACATGGGAACTGTCCACCAGGA GGTTCCTCAGAGGCTCAGTGCTCGGGGCTGTTATTGGGGAGTAGTCATGTGGCCCCCTCTGTCTGACATG TATCCGCATCCCAGGTTCCCCAGAGGAAAGCAGGTGTTGCACAGAAACCACAGTGATTGCACAAGCAGTT TAGGCTCAGCGAGCCCCACTCACTTAGGGGATTCTGGGAGCCCTCCCGGAATCCAGGGTCCCAGACCTTG CCAGGAGAGCCGTCTCAGGCCTGCTGCATTAGCCCTTCCTGCATGGCTGGATAGTGACAGAGAGAAAGAG GAAGAAGAAGCTTATTTAAAACTAGGAAGCAGCAGGTCGTGAGAGCCAAGAGTGCGCTTGACCTTGTTGG AACCAGGGCACCAGAGCCGCTTCTCATTGCTGGGAAGTTTGGGGATGATGACCCTGACCTTCCGCGTGGT TATAACCTCACTCCCTTGTTTGTTCCCCCACAGCTGCACCCACCTCCACCCTCACCTGTGACTCAGGACC ACAGAAGCAAAAGTTCTCACTCAAACTGGTAAGTGACCCTGCAGGTGGGGGGCAGCTTGGAGGGGAGGGA CTTTCACAAACACATCCTTGACTTCTGAGCCCGTCTGTCAGCTGGGAGAGCTCGGCCGGGGGCCAGGTTC CCCACCTGCAAGGAGTATTAAGACCGTGGGCGGGGCAGGCGCCCAGTTCTCCATCATCCCAGGGTGTTGG AAACCCACAGTTGGATCCACCCAAGTCTGGCTCTGATGCTTCCCTGCTGTGTGACCTGGGCAAGCCCATC CCCCTCAGAGCTCAGTTTCCTCATCTGTGAAATGGAGCCACTAATACCCACAGGGTGTGGCCCATCTGCC CCCTACCACCGGCCTCGCAGCGTGCTCGTATCGAGCACCTACTGTGTATCACCGTGCTGGGTGTGGGGTC CAAAAGAGAACCCAGCAGATGGGGTCCCTGTGCTGAGGATTCAGTGAAATCACACATCGGGTGCCGAGCA TGAGTCTCAGCAGACAGGACATGTGCACTCGAGGTTGGGGCTAAAAGGAAACAAATAGAGAAAGGGAGAG TGGCCACGAGGGTGAATCACGCCTTCCACACAGAGGAGGTGCCTAACAGAAGGGCATCGTGGTGGTTCAT AGCCCCGCCATCATCCCTGGGTGCTGGGATTACAGGTGACTCTTATTTTCTTTTTGTTTATCAGAATTGT CTCACACCCCAGAAAACCAGGGACAGCTGGGTCAGTTGCCCTCCCTCCGCCCTAGGTGGAGGCCATGAGA GTGGGTCAGAGCGTGGGCTGTGGAGCCGGGCTCACTAGTGGTGGGTAGCACCTGCTTTGAGAGCCTGGCA TTGCCTGAGCCGGGCTGTGTTTCATAAGCACTGCTTCATTGAATCCTCCCAGTAGGGCAGCTACCGTGTC CCATTTAACACTTCTCTGCATGGTTCAGGTGTGTCTGGCACAGTAGGCACCCCATCAACCTGAGCTGTTC CTGTTATTACTACTGTAATTATGCTCATCATCCTAGAAATACAGCCTTGCTCTCTGTTCGTGCATCTGAC CTCATCAACATTTCCTGAGGCTCAAAACTTGGTGATGATGAGTTGGCCCCAGGCCCAGCCCTGGAGAGGC TCCCAGTCTGGCGGGAGCCATGTCATGTCCCAGTCCCATCGGGAAGAGGGGTGGGCTGGGAGGGGAGCAC AGGGCACTGGGCCAACCAGAAGACATTCACCCTCCCCATCCACTGGGAAGGCAGCTCTGTTCTGAAGCAT TCGAAAGGTCAGGGGCTGAATGCCCAGTGGTGTTCATCCATCCATTTATTCATTTATTCACCAAGCCTTT CCTGCAACCTCCTGTGAACTGAGTGCTGGGGACATCTGCAGAGATGGGTCAGACCCAATTCCTGCCCTGA AGGAGCTGCCCCCTTTGGGGAGTGGGGCAGGCATGAATACCCCACATGAGATTGCTCAGGGCCTGCATGG CTGTGCCCACGTCGCTCGCGTCAGTCACTATGGGCTTCACCAGCATCGTGGGGAGGACTCCAGGACGCAA AAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK074461 unedited CAGAATTTTGTAATACGACTCACTATAGGGCGGCNCGCGATTCGGCACGAGAACCTGGGT CACCCTGGGATGTGGCCTTGGATGAAGTCACTCTGCCTAGGACGCACCTAATTGCCCAGC CACAGGCTGTGACAGTACATGGGAACTGTCCACCAGGAAGTTCCTCAGAGGCTCAGTGCT CGGGGCTGTTATTGGGGAGTAGTCATGTGGCCCCCTCTGTCTGACATGTATCCGCATCCC AGGTTCCCCAGAGGAAAGCAGGTGTTGCACAGAAACCACAGTGATTGCACAAGCAGTTTA GGCTCAGCGAGCCCCACTCACTTAGGGGATTCTGGGAGCCCTCCCGGAATCCAGGGTCCC AGACCTTGCCAGGAGAGCCGTCTCAGGCCTGCTGCATTAGCCCTTCCTGCATGGCTGGAT AGTGACAGAGAGAAAGAGGAAGAAGAAGCTTATTTAAAACTAGGAAGCAGCAAGTCGTGA GAGCCAAGAGTGCGCTTGACCTTGTTGGAACCAGGGCACCAGAGCCGCTTCTCATTGCTG GGAAGTTTGGGGATGATGACCCTGACCTTCCGCGTGGTTATAACCTCACTCCCTTGTTTG TTCCCCCACAGCTGCACCCACCTCCACCCTCACCTGTGACTCANGACCACAGAAGCAAAA GTTCTCACTCAAACTGGATGCCAAGGATGGGCGCTTGTTCAATGAGCAGAACTTCTTCCA GCGGGCCGCCAAGCCTCTGCAAGGTACCTGACAGGGAACTGNNGCAGGANGGGAAAGTGA GGGGGGCGCCACTTGGTCACAGCACTTGACTTCTACCTGCAGCATGANAAGGGTGGGCTA NATTAAGGGCCCAGTTGCTCCATCTGTGCCATACCTGACTCTGA
Restriction Sites	NotI-NotI
ACCN	AK074461
Insert Size	2600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK074461.1
RefSeq Size	2110 bp
RefSeq ORF	2110 bp
Locus ID	51231
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. In both human and mouse, this gene has substitutions at several residues within the ATP binding motifs that in other kinases have been shown to be required for catalysis. In vitro assays indicate the protein lacks phosphorylation activity. The protein, however, likely retains its substrate binding capability. This gene is widely expressed in human tissues and its protein localizes to the nucleus. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]

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