Lipin 3 (LPIN3) (AK092916) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK092916, the custom clone sequence may differ by one or more nucleotides TTTCAGGCTGAATGACAAGACTCCGCGATGCTTCTCGGCCTGGGCTGCACAACGGAATCTACCAGGGAGG TTTTAAAAACACTGGTACTTGCTGGAGGTGGTGGCGCCTGTCTGTAATTGCAGCTGCTCCAGAGGCTGAG GCGGAGGATTGCTTGAGCCCAAGAGTTTCGGTTTATTTGCTTTTTCAGAGTCTTGCTCTTGCCCAGGCTG GAGTGCAGTTCCATGTTCTCGGCTCACTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGTGCATACAAC CAGGCCTGGCTGATCTTCGTATTTTTAGTAGAGATGGGGTTTCACCATGATGGTCAGGCTGGTCAAGATC TCCTGACCTCAAGTGATCTGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGACATGAACCACAACAC CTGCCGAGCCCAGGTGTTTTGAATCCAGCACGGGCAATGGCAAGACCCTGTCCCAACAAACAAAAAAACA CTGGTGCCTGAGGCCCACCCCGAGAGATTCTGATTGAGTTGATTTGGATTTGAATTATCTTTAAAATTTT GGATGTGAATTTTTTTTTTTTTTCTTGGAATGGAGTTTCACTCTTGTTCCCCAGGCTGGAGTGCAACGGG ACGATCTTGGCTCACCGCAACCTCCGCCTACTGGGTTCAAGCCATTCTCCTGCCTCAGCTACTTGGGAGG CTGAGGTCGGAGGATCGCTGGAGCTTAACCTTGAGGTCGAGGTTGCAGTGAGCCGAGATTACATCACTGC ACTCCGGCGTGGGAGACAGAGACCCTGTATTAACAAACAAAACACAAACCACAAAGGGCAGGTCTGAAAC TGCCATTTAAAAAAAAATTTAATAAACTTAAAAAAATATATATCCACAGATGCAGGTGAAGAACCTGTTG TCTTCCTCAAGCCTCTTTTTCACCCATGGGTGGAAATGGTGCCCTGGACACCCAGGCCCACGAGGTCTTT GCGTGGGGTCCCTACACAGGGCTTTAGCTTACACTGTGCTGCCCTCCTGTCCCCCGAGTTCCCAGTCTGT CAAAATCCAACCTGGTCTCCCAGGCCCAGGGCAAATGCCACCTCCTCCATGAAGCCTGCCACATCCTTTG CACACCCTTGGGCGCTGACCTTGTTCTCCCAGCGCACAGGCACGGGTACAGTTTGCCCCTGTAGTAGTAA CTCAGGCACAAAACGAACTCTTGCTGAGGCTCGGCCGCGCAGAGCTGAGGGTTGCCGCTTCCAGGTTCAA GTGCGTTTTGAGTTTCATTCCCAGCTTCCTTCTTTTTCTGGTCTTTAATTTCTTCTCCGGATTAGGTCCC ACTCAATGCTTTCCTTCTCAATTTCCAAAAGAGTATGGTCAGAGCCAGCAGCACACCACCTTCCCCATGG GTGGGGGGGGGCCAGCCTGTGGCGGGGGTGCGGGTCCCATCTTTTCGAAGGAATTGACCCACAGTGGGCG GGTCCACCTTTGACCTTGCCCCAGGGAGCGCAGACAGAAAAAAGATCCTTGCTTAGTTTGAGGGGCCGCT GGGGTGCTCGGTTTGTCTTCAGAGGCCTGTCTGTAACACCAATGCCAACCCGGTGGCACTGACTGGTCAC CCTGAAGGCCACGGCCAGTGTCCTAGGAAGGGACTCAATTTCTAGCTGTGCCACCTGAGATTCTGGGGTT AGGCTGGTTGTGCTTCTGAAGTTCCACTGTGCTCAAAGTGCTTGGTGAAAGTTAGCGAAGGTGATTTTAC AAAAATAGATGCATAAAATGTCTAGGAAACACAAAAAATCCTCATTACTCTTCTCTCCAAATATTTTTTA AGCCCCAACTGGACCCTAGGCAAAAGTGAGTGGCACTCCTCTGCCAGGACTCCAGGCAAGCCCCGGCATC TTCTTGCTGCCGTCCCAGACAACAGAAGTTACCAGATGAACAGACTTGGATGGGCCACGGGGGTGGAGAG CTGGAAAGCTTGGCTGTGCCTCTCGATGATGATTAAGATTTCAATATTTACAGCAAAACCACAAAGCAAA TGATAGAATAAAGCAAAACAATGGAAAATCTGAGTTCACTCGTGAGAGAGGTACGTATGTGAGCTCTGAG GAAATTACAGAGGGAACGCATGCAGCGGGACAGCTCTCCCAATCGCAGCGTGCAAAGTAGACATCCATAG TGTCTTTTGAAAAATGAAAAACACATTACTTTGAACAGCCAAGAAAAAAATTGCAATTTATTAAGATTCA ATAAAGCGTTGTACTTTCGAAAGC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK092916 unedited GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCAGCAACAACAACAAC AACAAAGGCTGGGCACCTGGCTCATGCCTGTATTCCCAGCAGTTCGAGATCAGCCTGGGC AACATGGTGAGACCCTATCTCTACAAAAATAAAAAATGAAATGAGCTGGGTGGGGTGGCG CATGCATGCCTGTGGTCCCAGCTACTTGGGAGGCTGAGGTCGGAAGATTGCTGGAGCTTA ACCTTGAGGTCAAGGTTGCAGTGAGCCGAGATTACATCACTGCACTCCAGCGTGGGAGAC AGAGACCCTGTATTAACAAACAAAAACACAAACCACAAAGGGCAGGTCTGAAACTGCCAT TTAAAAAAAAATTTAATAAACTTAAAAAAATATATATCCACAGATGCAGGTGAAGAACCT GTTGTCTTCCTCAAGCCTCTTTTTCACCCATGGGTGGAAATGGTGCCCTGGACACCCAGG CCCACGAGGTCTTTGCGTGGGGTCCCTACACAGGGCTTTAGCTTACACTGTGCTGCCCTC CTGTCCCCCGAGTTCCCAGTCTGTCAAAATCCAACCTGGTCTCCCAGGCCCAGGGCAAAT GCCACCTCCTCCATGAAGCCTGCCACATCCTTTGCACACCCTTGGGCGCTGACCTTGTTC TCCCAGCGCACAGGCACGGGTACAGTTTGCCCCTGTAGTAGTAACTCANGCACAAAACGA ACTCTTGCTGANNGCTCGCCGCGCAAAGCTGANGGTTGCCGCTTCCAGTTTAAGTGCATT TTGAGTTTCATTCCCAGCTTCTTCTTTTTCTGGCTTNAAAATTTCTCTAGGATTAAGTCC CACTCATGCTNTNCTTCTCAATTNCAAAGAGTATGGTCAGAGCCAGCACACACCACCTCC CATGGTGGGGGGGGGGCCAGCCTGTGCGGGGGTGCGGGTCCATCTTTCGAAGAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK092916 unedited NGGTATGACTAAGNNACCGCGGCCGCATTCTAGNGATCGATTTTTTTTTTTTTTTTTTGC TTTCGAAGTACAACGCTTTATTGAATCTTAATAAATTGCAATTTTTTTCTTGGCTGTTCA AAGTAATGTGTTTTTCATTTTTCAAAAGACACTATGGATGTCTACTTTGCACGCTGCGAT TGGGAGAGCTGTCCCGCTGCATGCGTTCCCTCTGTAATTTCCTCAGAGCTCACATACGTA CCTCTCTCACGAGTGAACTCAGATTTTCCATTGTTTTGCTTTATTCTATCATTTGCTTTG TGGTTTTGCTGTAAATATTGAAATCTTAATCATCATCGAGAGGCACAGCCAAGCTTTCCA GCTCTCCACCCCCGTGGCCCATCCAAGTCTGTTCATCTGGTAACTTCTGTTGTCTGGGAC GGCAGCAAGAAGATGCCGGGGCTTGCCTGGAGTCCTGGCAGAGGAGTGCCACTCACTTTT GCCTAGGGTCCAGTTGGGGCTTAAAAAATATTTGGAGAGAAGAGTAATGAGGATTTTTTG TGTTTCCTAGACATTTTATGCATCTATTTTTGTAAAATCACCTTCGCTAACTTTCACCAA GCACTTTGAGCACAGTGGAACTTCAGAAGCACAACCAGCCTAACCCCAGAATCTCAGGTG GCACAGCTAGAAATTGAGTCCCTTCCTAGGACACTGGCCGTGGCCTTCAGGGTGACCAGT CAGTGCCACCGGGTTGGCATTGGTGTTACAGACAGGCCTCTGAAGACAAACCGAGCACCC CAGCGGCCCCTCAAACTAAGCAAGGATCTTTTTTCTGTCTGCGCTCCCTGGGGCAGGTCA AAGGTGGACCCGCCCACTGTGGGTCAATTCCTTCGAAAGATGGGACCGCACCCCGCCACA GGCTGGCCCCCCCCCACCATGGGAAGGTGGGGT
Restriction Sites	NotI-NotI
ACCN	AK092916
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK092916.1
RefSeq Size	2264 bp
RefSeq ORF	2264 bp
Locus ID	64900
Gene Summary	The protein encoded by this gene is a member of the lipin family of proteins, and all family members share strong homology in their C-terminal region. This protein is thought to form hetero-oligomers with other lipin family members, while one family member, lipin 1, can also form homo-oligomers. This protein contains conserved motifs for phosphatidate phosphatase 1 (PAP1) activity as well as a domain that interacts with a transcriptional co-activator. Lipin complexes act in the cytoplasm to catalyze the dephosphorylation of phosphatidic acid to produce diacylglycerol, which is the precursor of both triglycerides and phospholipids. Lipin complexes are also thought to regulate gene expression as transcriptional co-activators in the nucleus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]

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