PAN3 (AK091307) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC102434 sequence for AK091307 edited (data generated by NextGen Sequencing) ATGAGAAATGATGTCATAGAGGAAGACCTTGCAAAGGAGGTTCAAAATGGAAGACTGTTT AGGCTCCTAGCAAAATTGGGAACAATCAATGAGAGGCCGGAGTTTCAGAAGGATCCCACT TGGTCAGAGACTGGAGACCGTTATCTGTTGAAACTCTTTAGGGATCATCTTTTTCATCAG GTGACAGAAGCAGGTGCTCCCTGGATTGACCTCAGTCATATAATTTCTTGTCTTAACAAG CTAGATGCTGGTGTGCCAGAAAAAATAAGCCTGATTTCCAGAGATGAGAAGAGTGTACTT GTGGTGACCTACAGTGACTTAAAGCGCTGCTTTGAAAATACTTTTCAAGAACTGATTGCA GCTGCAAATGGTCAGTTGTAG Clone variation with respect to AK091307.1 94 g=>a 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK091307 unedited TTCACATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACCAGGTTTGGGTTATC TTGTTTTTTAAATTGCATGTTGGACATCCAAGTAATTCCTCTTTTATCTTGGGTAGGTAT TTGTTGACTGACCAAAACAGGATGCGAAGTGTAAATGACATCATGCCCATGATTGGTGCT CGATTTTATACTCAATTGGATGCTGCTCAAATGAGAAATGATGTCATAGAGGAAGACCTT GCAAAGGAGGTTCAAAATGGAAGACTGTTTAGGCTCCTAGCAAAATTGGGAACAATCAAT GAGAGGCCGGAGTTTCAGAAGGATCCCACTTGGTCAGAGACTGGAGACCGTTATCTGTTG AAACTCTTTAGGGATCATCTTTTTCATCAGGTGACAGAAGCAGGTGCTCCCTGGATTGAC CTCAGTCATATAATTTCTTGTCTTAACAAGCTAGATGCTGGTGTGCCAGAAAAAATAAGC CTGATTTCCAGAGATGAGAAGAGTGTACTTGTGGTGACCTACAGTGACTTAAAGCGCTGC TTTGAAAATACTTTTCAAGAACTGATTGCAGCTGCAAATGGTCAGTTGTAGTATTTGCTA AAAAAGCACGCAGGACATGGCTAAAGACCTTAACCAATAGCAAATTGCACTACAGCTGAA CTTTTCATCATCTCATTCACATTTGGGAAACGAACAGGAGATGAGCAAAGCTGCTTGCAC TTCAGTCAGGTACACTGTTACTTGAAAGGAAGAATGTTTCACTTACCCAAGAGCTATGGC TGCCATTGGAGGCTGTTATCTGTGAAGAATTTATTTTAGATTTANGAGCACCATCAGGTG AATGGATGTCCTGCTTTTTGTTTTTCCACTTGTATATGCACTAANTTTAATTTNTTAAAG ACTTTTCTGATCTTTGAACTTTGCACATTGATACTTACATGGNAAACTTGCAGGNCATTT TTGGAAGCATGCG
Restriction Sites	NotI-NotI
ACCN	AK091307
Insert Size	3500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK091307.1, BAC03632.1
RefSeq Size	3208 bp
RefSeq ORF	381 bp
Locus ID	255967
Protein Families	Druggable Genome
Gene Summary	Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins.[UniProtKB/Swiss-Prot Function]

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