TMEM120B (AK094258) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK094258, the custom clone sequence may differ by one or more nucleotides TCTTTCCTGTATTTCTCTTTTATACTAGAATATTAAAAAGCCAGGTATAATGAAGTGCATAGGAGCTAAA TAGAAGCAGCTTTGAATACCTTCATTTTATGTAGATGTTCCAAGAGCTTGCCCAGGCTTCCTCTCTCTCC CTTCCTCTCTCTCCCTTCGCCTGTCGAGTCTAAGGAGACCTGGCAGCCTGAGATACCCTTGGGTGTTCAG GGAGATCTTGAAGGCAGGCGCCTTTGAGGTACGCACCTACCAAAAATGTCACATCACCACCGGTTCTGTT GTTTCACATTGAATGTGAAAGATTCACTAAAAGGTTAATTTCCAGTCTTGTCAAATGTAGATCATTAAAG GCATTGCCTTTTCATGGTACAGCTGACAAAAGTCAGGAGCCAAGAGGGAAGGCAGATGCAAAGGTTGAAT AGGCCAAATACTGTGAGAAGCCCTTTGGAAAACCACATCAGGTATTTTTATCTGTACTCAGCAGAAGTAG TGATTGGTGACTATTTGCGGAAGGAAACTGGACGGGTGTCTGCCTTTTTAAATGTTAAAATAGGCCCTGT CTATTCTAGGAATGGTTTTGATAATTATTTTTTCATTCTTTGCCTTTATTCTAGAATAGAGATTGCCCAT ATTTTAAAAAAATGCTTTTCATGTTTCTATTTGTTTTGATTTCTAGAACCATGTTTAGATGACAAAAACA CCAGATTGAAGGATGGTCCATCTGGTATCTTTGGCTTTCATAAAAGCCAGTTTGAATCACATCATGGTTG ATCCAATGGCTGGAAAACACATATTTACCCAAAATATGGTTAATTCCGTACTTCTGAATAATCCTGGGTT TTGACTTCTTCAGTTTCTCCCCTTTTTTACTTTCTTCTGTTTGAGAGGCACTGTGAATAGTGGAAGGAGC ATGGGATTCAGAGAGTTCCAGATAGGAATTTTAGTTTTTCCATTTAAGAGCAACTGCCTTTGGACAAGAT GCTTAACGTCTCTGAACCTCAGCTTCTTCATCTAATGGAAGGATGCTAATAACCACCTCACAGGATTTAG GGTAGTGCTAAATGAGAAAGCCCATGTAGGAATACTTACTGTCGGAGCCAGCAAATTCATAGCGCACACT CCAAAACGGGTGCTCACTATTGACAATGTCATTTCCTGGTTTCTTATTTTTTGATTAGCTCTTTTCTTTA ATCTGCTTTCATTCTTAATATGTCTCTTTTCCTTTTTAAAGCATAGAAGTAGTATGTGGGTCTTTTATGA ATTTGGGTTGATAGCTGATTAATTGAATATTTAAGAGTTGTTTATTGCTTGTAAACAGCATGGTTTTCTA AGACATTGCATTTAATTAAAGCATGCTTTTCAATAACACTAGTTACCTTGACCAAACTGGAGTTACTGCT TCACATAAAGGACTATTACCTGTCAAGAGACATTTACTCTGCTGGATGAAGAACTGAGGAAGAGTGTCAT GACGTTAATTATTTAAAGGCACTTTTCTGAAAACATGTCTCAAATCCTAATGATTCTGTTCTTTGCCTAT AAACATTAGCGATGCCATTCTACACTTTTCACTGAAATGGTTCTAAAAATATAGGAGGGAAATTTTGATG TGTGATAAAAGTTCAGGACGATCACATTTTTCTGCCTGTGTACTTTTGCTTAGTATTACTTTGAAAAACC AGCTGGAATTTTTGAAGCAGCCTCCGTTTTGCTAAAGTCATTTTGTAGGGTGGGCCTAATAATGGCCTGG CACAGGACCTGAACATCGAAAACACCTAAAGAACCTGACACACCTGCAAGGCATTGTAGGGGCGGGAGAG ACGGTAGAGAAGTGAGATGAGTGTGTTTTCTTTGGGAAAATAAGTGATTTTTGCTGAACAGCTTAAGGCA CTCTGTAAACATTAGCCAAAAAATAAATATTAATTCTCACTGTGGCCTCGGTCCCTTAGTGAAGCCATGC TTACTCCAGGAACTGCTGCGATGAAGAGTGGTGAAAATTCTGAATGAAAAGCAGCCCTCAAAAATGTTAA AACCCATGCTATTAAGCTTTACACCAAAAAATAAATTCGAATTTTTAAAAAGTGAGAAATGGGGAAAAGA AGAGGAGGCTTAGGATGGTCAAGTGGCACCTTTAGCACCTTGTACTTAACCAGATAAGTTCAAGACTGTG TTTTAGGCCGGGCACGGTGGCTCACACCTATAATCCCAGCACTTTGGGAAGCCAAGGCAGGTGGATCCCT TGAGCCCAGGAGTTCGAGACCAGTCTGGGCAACATAGTGAAACCCTGTCTCTACTAAAAATACAAAAATT AGCCAGACGTGGTGGTGTGCACCTGTAGTCCCAGCTATTCAGGAAGCTGAGGTGGGAGGATTGATTGAGC TCAGGAGGTGGAAGCTACACGGAGCCGTGATCTCACCCCTGCACTCCAGCCTGGGCAACAGAGTGAGACG CTGCCTCACTGCTCCCCCACC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK094258 unedited ACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGCATTGGGGGGCTTGACAAT AAGAAACAATATTCTGACTCTTTCCTGTATTTCTCTTTTATACTAGAATATTAAAAAGCC AGGTATAATGAAGTGCATAGGAGCTAAATAGAAGCAGCTTTGAATACCTTCATTTTATGT AGATGTTCCAAGAGCTTGCCCAGGCTTCCTCTCTCTCCCTTCCTCTCTCTCCCTTCGCCT GTCGAGTCTAAGGAGACCTGGCAGCCTGAGATACCCTTGGGTGTTCAGGGAGATCTTGAA GGCAGGCGCCTTTGAGGTACGCACCTACCAAAAATGTCACATCACCACCGGTTCTGTTGT TTCACATTGAATGTGAAAGATTCACTAAAAGGTTAATTTCCAGTCTTGTCAAATGTAGAT CATTAAAGGCATCGCCTTTTCATGGTACAGCTGACAAAAGTCAGGAGCCAAGAGGGAAGG CAGATGCAAAGGTTGAATAGGCCAAATACTGTGAGAAGCCCTTTGGAAACCACATCANGT ATTTTTATCTGTACTCAGCAGAAGTAGTGATTGGTGACTATTTGCGGAAAGAAACTGGAC GGGTGTCTGCCTTTTTAATGTAAAATAGGCCCTGTCTATTCTANGAATGGTNTTGATATN ATTTTTTCATTCTTTGCCNTTATTCTAGAATAGAGATTGCCCATATTTTAAAACAAGCCT TTCATGTTTCTATTTGGTTTGATTCCTAGAACCATGTTTAGATGACCAAAACACCAGATT GAAAGATGCCCCATCTGGGATCTTTTGGCTTTCATAAAGCCAGTTTGATTACATCATGGT TGATCCAAGGCTGGAAAACACATATTACCCAAATATGGNTAATTCCCCACCTCTGATATT CCCGGGTTTGACTTCTTAANTTCTCCCCTTTTTACTTCCTTGTGAGG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK094258 unedited NTCCTGATGNATAGGNNCTACTCTGGNATNCGATTGAGGACGGCCCCCTTNNNNGGGGGG GGAGTAGTGAGGCTGCGTCTCACTCTGTTGCCCAGGCTGGAGTGCAGGGGTGAGATCAGG GCTCCGTGTAGCTTCCACCTCCTGAGCTCAATCAATCCTCCCACCTCAGCCTCCCGAATA GCTGGGACCACAGGTGCACACCACCACGTCCGGTTTTTTTTTGTATTTTTAGTAGAGACA GGGGGGGGAGCATGCTGCCCAGACCGGTCTCGAACTCCATGGCTCAAGGGATCCACCCGC CTCGGCCTCCCAAAGTGCTGGGACCACAGGCGTGAGCCACCGTGCCCGGCCTAAAACACA GTCTTGAACTTATCTGGTTAAGTACAAGGTGCTAAAGGTGCCACTTGACCATCCTAAGCC TCCTCTTCTTTTCCCCATTTCTCACTTTTTAAAAATTCGAATTTATTTTTTTGGTGTAAA GCTTAATAGCATGGGTTTTAACATTTTTGAGGGCTGCTTTTCATTCAGAATTTTCACCAC TCTTCATCGCAGCAGTTCCTGGAGTAAGCATGGCTTCACTAAGGGACCGAGGCCACAGTG AGAATTAATATTTATTTTTTGGCTAATGTTTACAGAGTGCCTTAAGCTGTTCAGCAAAAA TCACTTATTTTCCCAAAGAAACACACTCATTTCACTTCTTTACCGTCTCTTCCGCCCCTA CAATGCCTTGCAGGTGTGTCAAGTTCTTTAGGTGTTTTTCATGTTCAGGTTCTGTGCCCA GGCGATTATTTGCCCCCCCTACCAAATGACTTTAGCAAAAGGGAGCTGGCTCAAAAATTC ACCTGGTTTTCACAGTATACTCTACAACAGACCCAGGCGGAAAATGCTTCTGCCTGGATT TTTTCAACATGAGAAATTCCCTTCGATTTGT
Restriction Sites	NotI-NotI
ACCN	AK094258
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK094258.1
RefSeq Size	2471 bp
RefSeq ORF	2471 bp
Locus ID	144404
Protein Families	Transmembrane
Gene Summary	Necessary for efficient adipogenesis.[UniProtKB/Swiss-Prot Function]

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