NWD1 (AK090685) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK090685, the custom clone sequence may differ by one or more nucleotides ATCGTCCTGGTGCTGGACATCACCTCCGGGGACCCCTGCCCGGTCATCGATGGGCCAAGATACACCTTTT ACACTCAGCTGCCCGAGACCCTCTCCAGCGTGGCCATTCTGACGGACTACCGCGTGGTCTACAGCATGAC CAATGGGGACCTCTTTCTTTACGAGTGTGCAACTTCCAAAGCGTTTCCCTTGGAGACCCACAGGAGCCGA GTTGCCTGTGTGGAGGTCAGCCACAAGGAGCAGCTGGTGGTCAGCGGGTCTGAGGATGCCCTGCTGTGTC TCTGGGACCTGCAGGCACGCAAGTGGAAATTCGAGATGAGCTACACGAGTTCTTACTGCAGAGGGGTCCA ATGTGCTTGCTTCTCCAAGGATGACAAGTATGTGTACGTGGGCTTAAAGGATCGCTCTATACTTGTTTGG AGTGTGCTGGATGGCACCCTGCTGACAGTCCAGTTTGTCCATGCTGTGGTAAACAGAATCATCCCAACCA CCAGTGGCTTCATTGCCCCCACCAGGCATGGTTATCTGATCCGAGAGAATTTCCAGTGCCTTTCAGCAAA AGCCTCACCGCAGGACCCTCTTAAGAACTTCAAGAAGGCAATGTGGATGGTCAAATCCAGGCAGAGAGAG GAGCTAGTTGCAGCTGCAGGAGCTCCCCAGGACTTGGAGTCAGAAAGTGCCCAGGGAAATGAAACCAAAT CAAACAAATGCTCACAAGTGTGCCTGATAGTGTAAAAAAATAAAAATAAAAAAACCCTGTGGGGGTATGG GGCTCCAGTGAGTTAGCCCCATTTAATCTAGTTAAAGCAAAAAGAATACGTTTGCTGGTGTATATCTGGA CCCATGGCTTCAGATTATGGCTTCAGACCTTGTCTCTTCTCATCTTTCCATTATTCTTTCCTCTTTTTCA TTTTCATTCTCAGACTTGCCACCCCCAGGTTAGCAACATGGTGGCCAATATGCTGCTGTCTCCAATTTCA ATAGAAAAGAGAGCTTCTCTTTCCCAACAGTCCTAGCCTCAACTCTACTGGTCCCAATTGGCCCATACGC CTAGCCCTAAACCAATTATTATATCTGGGAGAATGCAGAGCTCTAATCGGCTAGACTGATCCAATGTCCA CCACTGGGACTGAGAATAGAAGAGATTACTCCTGAGATGCAAACTGGAGTTCTGGTACCAGATGAAGGGG GAACGGATGAATGTGAGATACAGCAAATCACTGATGTGGACCACAGAACCTCACAGTTTATAAAGCTGTA GATGCTGTCATCTGATCCTCATCACAACTCATACTTTAGGGAGGGCAGGAGTTTCTGTATCTATTTTACA GATGAGGAAACTGAGCCTTGCGGAAGTAGTTATAATTTGATTCAGGTCACAAGTCCAAGGTGCAAGAGCT CTGAGAGTGAGCCTTCTGTCCCATTCCTGTTAGACCTCACTCCAATTTCAGTCTGCAGAGCACAACCATC TCTTCCACAGGCACCACACTCAAATGCTCACAGGAGCTAGTGACTAATCTAGACACTTCCATGCACCTGG GTGAGCAGCAGGGAGAGACAGAGACTGGACATGTGGAAGGCAGAGATTGTGTCTAGGGTGGGTATGAGCA GCCTGACTGTAGCCCAGAATCACCATGGCCAAACAAGCCCACTGCTGTCAGTGCTTCCAGTTGTAATAAG CAAAGCCAGAAATCCAAACTTTTATGCAAAAATGTTCTGACTTTTAAAGTTGGCGATTGGTTGAATTTAT ATCTATAGTGCATTTGGCATGTGCGTCTTTGGTTAATGAGCTGACTTCAGGACTCACGCTGGCAGTAAAA CAATCAACATGTTATAGTTCATCCCTCTCTATTCCTTGGCTCCCAAACTTTTGGGATAAAGATGTGGGTT TTTAATCTTAAAAACTGGAGGCTCTTGGAAGTAGAAAGAGCTGCTGATCTCTCCCTGCTTTGCTTTTGAA CTGTCTGCCTTGTGCAAGTTCAACAGTCAAACTGTTTTCATTCCACTTAAGTGGATCTTTCTTCTTTGCC CACAGCCTTCTAAAAGCCCTTCTCAGTCTTCTCAGGCTTTTACTTTAATGCTAGGATAGTCTAAATCTTC AAGGTGGCTATCCCTTCTCCCCTCCGTGGAATTCCTGATAGAGTCTCAATAAAAGCAACCTTAGAAACAG AGGCTTTTGAGCTGAACAAGACCCTACTGCTGTCTTATTCATCCATTTTCCAAACCAGTTTTCCAAGGCA GGTTTTGGTAGTTCTGCAAATGTTAGTGTCAAATTTCATTTAAAAATATTTTAAACTGTAATTTTAAGAT GTATGCTTGGATGTATTGGGCAGCAGCTTCTAAGGTGTTGAAGATTACTAAGGTTGGTGCAAAAAGTAAC TGTGGTTTTCGCCATTAAAAATATTAAAAATAATGGCAAAAACTGGCTGGGCACAGTGGCTCACACCTGT GGTCCCAGCTCTTTGGAAGGCCGAGGCAGGTGGATCACCTGAGGTCAGGAGTTTGAGACCAGCCTGGACA ACATGGTGAAACCCTTCTCTACTAAAAATACAAAAATCAGCCAGGCATGGTGGTGAGTGCCTGTAATCTC AGTTACTCAGGAGGCTGAGTCAGGAGAATCACTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCTGAGATT GTGCCACTGCACTCCAGCCTGGGTGACAGAGCAAGACTCCATCTCATAATAATAATAATAATGGCAAAAG CCTCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCGGGTGGATCACTTGAGGCCAGGT GTTCAAGAGCAGCCTGGTCAACATGGTGAAACTCTGTCTCTACTCAAAAATACAAAAATTAGCCTGGTGT GGTGACGTACACCTGTAATTCCAGCTACTTGGGAGGCTGAGGCAGGAGAATGGCTTGAACCCGGGAGGCG GAGGTTGCAGTGAGCCGAGATCGCACCACTGCACTCCAGCATGAGTGACAGAGACTCTGTCTCC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK090685 unedited GTCCTATTATGTAATACGACTCACTATAGGGCGGCCGCGAAATTCGACGAGGTCACCGCA GTGTCCCACAATGGAAGCTACGTCTACTTCCCCAAATTGGGGACAAAAACAAAGTCACTA TTTGGGACTTGGCAGAAGGCGAGGAACAAGATTCCCTGGACACCTCCAGTGAGATCAGGT GTCTGGAGGTTGCTGAGCAGCGCAAGCTCCTATTTACGGGCCTCGTGTCGGGGGTCGTCC TTGTGTTCCCCCTGAATTCCAGGCAGGACGTGATATGCATTCCCCCTCCCGAGGCCCGGA AAGCAATCAACTGCATGTCCCTGAGCAAGTGCGAGGACCGCCTGGCCATCGCCTATGACA ACATCGTCCTGGTGCTGGACATCACCTCCGGGGACCCCTGCCCGGTCATCGATGGGCCAA GATACACCTTTTACACTCAGCTGCCCGAGACCCTCTCCAGCGTGGCCATTCTGACGGACT ACCGCGTGGTCTACAGCATGACCAATGGGGACCTCTTTCTTTACGAGTGTGCAACTTCCA AAGCGTTTCCCTTGGAGACCCACAGGAGCCGAGTTGCCTGTGTGGAGGTCAGCCACAATG AGCAGCTGGTGGTCAACGGGTCTGAGGATGCCCTGCTGTGTCTCTGGGACCTGCAGGCAC GCAAGTGGAAATTCCAGATGAGCTACACCGCACCCTGCTGACAGTCCAGTTTGTCCATGC TGAGGGAAACAGAATCATCCCACCACCAGTGGCTTTATTGCCCCCCCAGCATGGTATCTG ATCCGAGAGATTTTCGTGCCTTTCACAAAACCTCACGCAGACCCTCTAAGAACTTAAAAA GCATGTGATGGTCAAATCCCCAAAAGAGGACTTTTCCCTGCAGAGCTCCCGACTTGATCC AAAGGCCACGAAAGAAACCATAACAAGCTCCAG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK090685 unedited NGGTAAATCCAGGACCGCGCCGCAATTTAGGATCGGTTTTTTTTTTTTTTTTTTGGGAGA CAGAGTCTCTGTCACTCATGCTGGGGTGCAGTGGTGCGATCTCGGCTCACTGCAACCTCC GCCTCCCGGGTTCAAGCCATTCTCCTGCCTCAGCCTCCCAGCAGCTGGAATTACAGGTGT ACGTCACCACACCAGGCTAATTTTTGTATTTTTGAGTAGATTCTGAGTTTCACCATGTTG ACCAGGCTGCTCTTGAACACCTGGCCTCAAGTGATCCACCCGCCTCAGCCTCCCAAAGTG CTGGGATTACAGGCGTGAGCCACCGAGGCTTTTGCCATTATTATTATTATTATGAGATGG AGTCTTGCTCTGTCACCCATGCTGGAGTGCAGNGGCACAATCTCAGCTCACTGCAACCTC CGCCTCCCGGGTTCAAGTGATTCTCCTGACTCAGCCTCACGAGTAACTGAGATTACAGGC ACTCACCACCATGCCTGGCTGATTTTTGTTTTTTTAGTAGAGAAGGGTTTCACCATGTTG TCCAGGCTGGTCTGAAACTCCTGACCTCACGTGATCCACCTGCCTCGGCCTTCCAAAGAG CTGGGACCACAGGTGTGAGCCACTGTGCCCAGCCAGTTTTTGGCCTTATTTTTAAAATTT TTAATGGGCGAAAACCACAGTTACTTTTTGGCCCGACCCTTTAGGAATTTTNAACACCCT TGGAAACCGCGTGCCCAATACATTCCAAGCCTACCTCTTTAAAATACCAGTTAAAAAATT TTTAAATGAAATTTGACACTAACTTTTGGAGAACTTCCAAAACCCTGCCTGGAAAACTGG TTGGGAAAAGGGTTGATTAAAACCGGGTTGGGGTTTTGTTAGCTCAAAACCTCTGGTTTC TAGGTGCCTTTTTCGG
Restriction Sites	NotI-NotI
ACCN	AK090685
Insert Size	3500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK090685.1
RefSeq Size	3004 bp
RefSeq ORF	3004 bp
Locus ID	284434
Domains	WD40
Gene Summary	The protein encoded by this gene is thought to be a cytosolic protein and predicted to contain a NACHT domain and multiple WD40 repeats. Increased expression of this gene was observed in some prostate cancer cell lines. Knocking down expression of this gene results in decreased androgen receptor protein levels, indicating that this gene may be important in modulating androgen receptor activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2016]

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