MRPL47 (BC032522) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CGI-204; MGC45403; mitochondrial ribosomal protein L47; nasopharyngeal carcinoma metastasis-related 1; NCM1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC032522, the custom clone sequence may differ by one or more nucleotides ATGGCTGCGGCCGGTTTGGCCCTTCTTTGTAGGAGAGTTTCATCCGCCCTGAAATCTTCCCGATCGTTAA TAACTCCTCAGGTCCCTGCCTGCACAGGATTACTTCATACCACATTGTCAAGGAAAGGACTAGAAGAATT TTTTGATGACCCAAAAAACTGGGGGCAAGAAAAAGTAAAATCTGGAGCAGCATGGACCTGTCAGCAACTA AGGAACAAAAGTAATGAAGATTTACACAAACTTTGGTATGTCTTACTGAAAGAAAGAAACATGCTTCTAA CCCTAGAGCAGGAGGCCAAGCGGCAGAGATTGCCAATGCCAAGTCCAGAGCGGTTAGATAAGGTAGTAGA TTCCATGGATGCATTAGATAAAGTTGTCCAGGAAAGAGAAGATGCCCTAAGGCTTCTTCAGACTGGTCAA GAAAGAGCTAGACCTGGTGCTTGGAGAAGAGACATCTTTGGAAGAATCATCTGGCACAAGTTCAAGCAGT GGGTTATACCTTGGCACCTAAATAAAAGATACAATAGGAAACGATTCTTTGCCTTGCCTTATGTGGACCA TTTTCTCAGACTGGAACGTGAGAAACGAGCCCGCATCAAAGCACGGAAGGAAAATTTAGAGAGAAAGAAA GCAAAAATTCTTTTAAAAAAGTTTCCACATCTTGCTGAAGCCCAAAAGTCAAGTCTTGTCTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC032522 unedited ATTTTGGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCCGGTTTGGCCCT TCTTTGTAGGAGAGTTTCATCCGCCCTGAAATCTTCCCGATCGTTAATAACTCCTCAGGT CCCTGCCTGCACAGGGTTTTTTCTTAGTTTGTTGCCTAAGAGTACACCAAATGTGACATC CTTTCACCAATATAGATTACTTCATACCACATTGTCAAGGAAAGGACTAGAAGAATTTTT TGATGACCCAAAAAACTGGGGGCAAGAAAAAGTAAAATCTGGAGCAGCATGGACCTGTCA GCAACTAAGGAACAAAAGTAATGAAGATTTACACAAACTTTGGTATGTCTTACTGAAAGA AAGAAACATGCTTCTAACCCTAGAGCAGGAGGCCAAGCGGCAGAGATTGCCAATGCCAAG TCCAGAGCGGTTAGATAAGGTAGTAGATTCCATGGATGCATTAGATAAAGTTGTCCAGGA AAGAGAAGATGCCCTAAGGCTTCTTCAGACTGGTCAAGAAAGAGCTAGACCTGGTGCTTG GAGAAGAGACATCTTTGGAAGAATCATCTGGCACAAGTTCAAGCAGTGGGTTATACCTTG GCACCTAAATAAAAGATACAATAGGAAACGATTCTTTGCCTTGCCTTATGTGGACCATTT TCTCAGGTGAGCACAAGCACCTTTAATAAAACATATTATCTTTATACCAGATGACATATG GTCATATATCATACCAGATGACATANAGAAAGCTTAAGCTGTCTTTAAATTNTAATTTCT GTTATTACATAAGTATTATAAAAAATAGGAAAGTCAGGCTGGGTNNGCGTGGCTCACACC NTGTATCCCACACTCTTTTGGGGAGGGCCG
Restriction Sites	NotI-NotI
ACCN	BC032522
Insert Size	1500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC032522.1, AAH32522.1
RefSeq Size	1155 bp
RefSeq ORF	1155 bp
Locus ID	57129
Gene Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene is immediately adjacent to the gene for BAF complex 53 kDa subunit protein a (BAF53a), in a tail-to-tail orientation. Two transcript variants encoding different protein isoforms have been identified. [provided by RefSeq, Jul 2008]

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