NXN (AK096168) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FKSG27; IRGQ1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK096168, the custom clone sequence may differ by one or more nucleotides CATATTAGTGTCTAAGCCCTGCAATCAGGCATGTCAGCCACGTGATGGAATGGGAGGGCTGCAGGGCAGC ACTGTCCAGTAGAAACGAAATGCAAGCCACATATGTCATTTTAAGTTTTCTTTTTTTGAGATGGAGTTTC ACTCTATCACCCAGGCTGGAGTGCAGTGGCACGATCTCGCCTCACTGCAACCTCCGCCTCCCAGGTTCAT GTGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATATGCCACCATGCCTGGCTAATTTT TGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGATCAGGCTGGTCTGGAACTACTGACCTCAGGCCAC CCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCGCCCAGCTAATTTTTGTATTTTT ATTTTATTTATTTATTTATTTTTTGGGAGACGGAGTCTTGCTCTGTCACCCAGGCTGGAGTGCAGTTGTG CGATCTTGGCTCACTGCAACCTCAGCCTCCCAAGTAGCTGGGATTACAGGCATGCACCATCATGCCCAGC TAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACTGTGTTGGCCAGGCTGGTCTTGAACTCCTGACCTC AGGTGATCCACCGGCCTCAGCCTCCCAAAGTGCTGGGATTATAGGCGTGAGCCACTGCACCCGTCCTAAT TTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGCTAAGCTGGTCTGGAACTCATGGCCTCAAGT TATCTGCCCACCTCAGCCTCCCAAAGTGCTGAGTAAGCCAAGTTTTCTAATAGCCACATTAGACAAGTAA AAGGAAACAGGTTAAATTCATTTTAACATGTTTTACTTAACCCAATGTATCCAAAATAGCATTTCAACAT GTCATCGGTTTTTTAGTTTTTTTTTTTTTTGAGATAGTGCTTCGCTTTGTTGCCCAGGCTGGAGTGCAGT GGCACAATCTCGGCTCACTGCAACCTCCACCTTCCAGGTTCAAGTGATTCTCCTGCCTCAGCCTCCCGAG TAGCTGGGATTACAGGCACCCGCCACCATGCCCACTAATTTTTGTATTTTTGGTTAGAGATGGGGTTTCG CCATGTTGGCCAGGCTAGTCTCAAACTCCTGACCTCAGGTGATCCACCCACCTCGGCCTCCCAAAGTGCT AGGATTACAGGCGTGAGGCACCGTGCCTGGCGTCATCGGTATTATTTAAATGAATTATGTTACGTTCTTT TGTGCTGTCTTCAAAATCTGTTATATATTTTACACTTACACCAAATCTCAATTACCATGGTACATTTTTA TCTGAAATGCTTGACCTTTATTTTGATTTCATAAAATTCATAGTTGGAGAAGTAGATTCACATATCCAAG TTGTTCCAATTATATAATAGTTTTCCAAAAACTGAGATGGGTGTCCATTTTTTTTTTAAGTAAAGATGCA GGTCTGGTTATGTTGACCAAGTTGCTGGGTTGTTTTGTTTTGTTTTGAGACAGAGTCTCACTTTGTCACC CAGGCTGGAGTGCAGTGGCATGACCTCAGCTCACTGCAACCTCTGCCTCCCAGGTTCAACTGATTCTCTT GCATCATCCTCCTGAGTAGCTGGGACTACAGGTGTATGCCACCATGCCTGGCTAATTTTGGTATTTTTCT CAGAGACGGGGTTTCACCATGTTGGGCATGCTGGTCTTGAACTGCTGACCTCAGGTGATCCGCCCACCTC GGCCTCCCAAAGTGCTGGTATTACAGGCATGGGCCACCACACCTGGCCTCAGCTGTTCAATTAAAAGTAA ATACAACTTAAAATTCTATGTTTCATTGGCAGTAGTGCAACATTAATACTGAGTAGCCACATGTGATTAG TGGCTATGGTATTGGACAGGGAAGGTACAGAATACTTCCATCAACATAGAAAATTCTATCAGTCTATCTC TAGGGGCAGATAGTCCTTCCACTGACTTGGGCAAGTCACTCTACAAATGGCATCTACCTCACATGGTTAT GGTGAGAATTCAGCGTATGTATGTACATGCAGGCACACAATATGCACACAGACACATAACATAGTACACC CTTTCCTGAAAAGCCTGACACATGGAGCTCAAACATGAGTGCCACCCACCCCTGGGCAGCACCAAGATGG CTCTAGTCTGGGTGCCTTTGTCTCACCCCCATGCCTTTGCTCGGAGTGTGCTCCTCATTTTTCTGCCACT TTGACCCTGTCTCTGATTTGGTCCTGTCTGACATCACTGCTATATGCTTTGCTCCTCTCAATTTCCTCTG CCCTCATGCCAGCAGGAGTCATGCCAGAGATCATATCTGAGAAAGCAAGACAATTTTGTGTGTGTGTCTG TGCCCATAGAGGAGTGCTGGTTGTGTTGATATAGTTGTAGATTGGTTGTGTTTACACAGTTGTATATATT GACACCCTTGAGTGTTATGACTTCTTTTGGGGGTGGTCGCCTTTTAAATCATAACTTTTAATGGGATTCC ATTTTAGTCTTTGTGAAGACATAAGGTTGTTGGCAGGCATCTGTCCCTGGGAGCATCCAAGCAGAAAAGA CTAAGACTCCCTTGTAGACAGATCACTGGCCGCCACTGAAGTGTGTCTGCATGGCACCACAGGGCTGGAA GACCCTTGAAGGCAGGAATTCAAGGAAATGTATGATGAATTTTGGCATTGCCATCAAAAGCAGAACAGGC ATGGAAAACTTGGGTGAGTGGGCGAGACAACCTCCTCACCACAGCAGAGTTCCATCCATGCCTGGATAAT GATGGAGGGATTTGTGTCCACTGCAGTGGGGAACCATGAAGGACACATCAAGGGTGTGGTTGGCCTGTGG TGCTCTTTGGAGGAATGAATAAAAATGAATAGAAATCCT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK096168 unedited GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAATATTCATCCATATC TTTACCCCATCATATTAGTGTCTAAGCCCTGCAATCAGGCATGTCAGCCACGTGATGGAA TGGGAGGGCTGCAGGGCAGCACTGTCCAGTAGAAACGAAATGCAAGCCACATATGTCATT TTAAGTTTTCTTTTTTTGAGATGGAGTTTCACTCCATCACCCAGGCTGGAGTGCAGTGGC ACGATCTCGCCTCACTGCAACCTCCGCCTCCCAGGTTCATGTGATTCTCCTGCCTCAGCC TCCTGAGTAGCTGGGATTACAGGCATATGCCACCATGCCTGGCTAATTTTTGTATTTTTA GTAGAGATGGGGTTTCACCATGTTGATCAGGCTGGTCTGGAACTACTGACCTCAGGCCAC CCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGCGCCCAGCTAATTT TTGTATTTTTATTTTATTTATTTATTTATTTTTTGGGAGACGGAGTCTTGCTCTGTCACC CAGGCTGGAGTGCAGTTGTGCGATCTTGGCTCACTGCAACCTCAGCCTCCCAAGTAGCTG GGATTACAGGCATGCACCATCATGCCCAGCTAATTTTTGTATTTTTAGTAGAGATGGNGT TTCACTGTGTTGGCCAGGCTGGTCTTGAACTCCTGACCTCANGTGATCCACCGGCCTCAG CCTCCCAAAGTGCTGGGATTATAGGCGTGAGCCACTGCACCCGTCCTAATTNTTGTATTT TTAGTAGAGATGGGGTTTCACCATGNTNGCTAAGCTGGTCTGGAACTCATGGCCTCAAGT ATCTGCCCACTNAGCCTCCCAAGTGCTGAGTAAGCCAGTTTTCTATAGCCCCATAGACAA GTAAAGGAACAGGTAANTCCT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK096168 unedited TCGGTACATCTATGNACCCGCGGCCGCAANCTANGATCGAGTTTTTTTTTTTTTTTTTTG GATTTCTATTCATTTTTATTCATTCCTCCAAAGACACCACAGGCCAACCACACCCTTGAT GTGTCCTTCATGGTTCCCCACTGCAGTGGACACAAATCCCTCCATCATTATCCAGGCATG GATGGAACTCTGCTGTGGTGAGGAGGTTGTCTCGCCCACTCACCCAAGTTTTCCATGCCT GTTCTGCTTTTGATGGCAATGCCAAAATTCATCATACATTTCCTTGAATTCCTGCCTTCA AGGGTCTTCCAGCCCTGTGGTGCCATGCAGACACACTTCAGTGGCGGCCAGTGATCTGTC TACAAGGGAGTCTTAGTCTTTTCTGCTTGGATGCTCCCAGGGACAGATGCCTGCCAACAA CCTTATGTCTTCACAAAGACTAAAATGGAATCCCATTAAAAGTTATGATTTAAAAGGCGA CCACCCCCAAAAGAAGTCATAACACTCAAGGGTGTCAATATATACAACTGTGTAAACACA ACCAATCTACAACTATATCAACACAACCAGCACTCCTCTATGGGCACAGACACACACACA AAATTGTCTTGCTTTCTCAGAATGATCTCTGGCATGACTCCTGCTGGCATGAGGGCCGAG GAAATTGAGAGGAGCCAAGCATATAGCAGTGATGTCAGACAGGACCCAATCAGAGACAGG GTCCAAAGTGGGCGAAAAATGAGGAGCACACTCCCCAGCACAAGCATGGGGGTGGAGACA AAAGCACCCCGACTAGAACCATCTTTGTGCCGCCCAGGGGTGGGTGGCACTCATGTTTGA GCCTCCTTTGTCAAGCCTTTCTCGAAAAGGGGGGACCATGTCATGTTGTCTGGGTGCCTA TTGTGGGCCCGCCCTGTCT
Restriction Sites	NotI-NotI
ACCN	AK096168
Insert Size	3250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK096168.1
RefSeq Size	2909 bp
RefSeq ORF	2909 bp
Locus ID	64359
Protein Families	Druggable Genome
Gene Summary	This gene encodes a member of the thioredoxin superfamily, a group of small, multifunctional redox-active proteins. Members of this family are characterized by a conserved active motif called the thioredoxin fold that catalyzes disulfide bond formation and isomerization. The encoded protein acts a redox-dependent regulator of the Wnt signaling pathway and is involved in cell growth and differentiation. [provided by RefSeq, Sep 2015]

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