RPUSD3 (BC032135) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AI527266; MGC29784; OTTMUSP00000028769; RNA pseudouridylate synthase domain containing 3
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC032135, the custom clone sequence may differ by one or more nucleotides ATGGACGGCCGCCGTGTTTTGGGCCGGTTCTGGAGTGGCTGGCGGCGGGGCCTGGGTGTCCGCCCAGTGC CCGAGGACGCAGGCTTTGGCACCGAAGCCCGGCATCAGAGGCAACCCCGCGGCTCCTGCCAACGGTCGGG GCCCCTCGGGGACCAGCCCTTCGCGGGGCTGCTGCCAAAAAACCTCAGTCGGGAGGAGCTGGTTGATGCG CTGCGGGCAGCCGTGGTGGACCGGAAAGGACCTCTAGTGACGTTGAACAAGCCACAGGGTCTACCAGTGA CAGGAAAACCAGGAGAGCTGACGTTGTTCTCAGTGCTGCCAGAGCTGAGCCAGTCCCTAGGGCTCAGGGA GCAGGAGCTTCAGGTTGTCCGAGCATCTGGGAAAGAAAGCTCTGGGCTTGTACTCCTCTCCAGCTGTCCC CAGACAGCTAGTCGCCTCCAGAAGTACTTCACCCATGCACGGAGAGCCCAAAGGCCCACAGCCACCTACT GTGCTGTCACTGATGGGATCCCAGCTGCTTCTGAGGGGAAGATCCAGGCTGCCCTGAAACTGGAACACAT TGATGGGGTCAATCTCACAGTTCCAGTGAAGGCCCCATCCCGAAAGGACATCCTGGAAGGTGTCAAGAAG ACTCTCAGTCACTTTCGTGTGGTAGCCACAGGCTCTGGCTGTGCCCTGGTCCAGCTGCAGCCACTGACAG TGTTCTCCAGTCAACTACAGGTGCACATGGTACTACAGCTCTGCCCTGTGCTTGGGGACCACATGTACTC TGCCCGTGTGGGCACTGTCCTGGGCCAGCGATTTCTGCTGCCAGCTGAGAACAACAAGCCCCAAAGACAG GTCCTGGATGAAGCCCTCCTCAGACGCCTCCACCTGACCCCCTCCCAGGCTGCCCAGCTGCCCTTGCACC TCCACCTACATCGGCTCCTTCTCCCAGGCACCAGGGCCAGGGACACCCCTGTTGAGCTCCTGGCACCACT GCCCCCTTATTTCTCCAGGACCCTACAGTGCCTGGGGCTCCGCTTACAATAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC032135 unedited NGGGGTCCCATTAGTATACTATCACTATAGGCGGCCGCGAATTCGCACGAGGGCTCGGGA GATGGACGGCCGCCGTGTTTTGGGCCGGTTCTGGAGTGGCTGGCGGCGGGGCCTGGGTGT CCGCCCAGTGCCCGAGGACGCAGGCTTTGGCACCGAAGCCCGGCATCAGAGGCAACCCCG CGGCTCCTGCCAACGGTCGGGGCCCCTCGGGGACCAGCCCTTCGCGGGGCTGCTGCCAAA AAACCTCAGTCGGGAGGAGCTGGTTGATGCGCTGCGGGCAGCCGTGGTGGACCGGAAAGG TGAGGGGTGAGAGGCACGGCCGCACAAGGACCGGGAGGGGACTGCCTCGGCGTGCAGTTG GGCTCTCCATTGAAGCCACGGGATCAGCCCTGCTTCTGCCTCCCACTAGCAGCATGAGCT GGGACAAGCCACCTAATTTGCACCCTCTCCCCGCTTTTAACCCTACTGCCATGATGCAGT AGGGTTAAGAGTGGGGAGAAGATGCAAATAACTACCGAGCCTTGCACATAGCCGGCGCTC AGTTACGGGAGTTGCTATACTAGATTATTGTTTATTGGCCGCGGTGGCTCACGCCTGTAA TCCCGGCACTTTAAGGTGCTGACGCGGGCGGATCACCTGAAGTCACGAGTTCGAGACTAG CCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAACACAAAAAATAGCCCACGTGTGG CGGCGGTCCGCCTGTTATCCCCAGCTACTTCGGGAAGGCTGACGGCAGTAANATCACTTT GAACCCCGATAGCATATGTTGCAGTGAAGCCGACATTGTGGCTACTGCGCGTCCACCCTG GGATGACAGAGCAAGACTCAATCTAAATAATTAATAACTAACTATGGTTTTTTTATCGCC GCACAAA
Restriction Sites	NotI-NotI
ACCN	BC032135
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC032135.2, AAH32135.1
RefSeq Size	1218 bp
RefSeq ORF	1032 bp
Locus ID	285367
Domains	PseudoU_synth_2
Gene Summary	This gene encodes a protein that functions in the assembly of the mitochondrial ribosome by adding a pseudouridine group to 16S rRNA. Loss of this gene results in causes defects in mitochondrial protein production. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2017]

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