SBF2 (BC027492) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CMT4B2; DENND7B; MTMR13
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC027492, the custom clone sequence may differ by one or more nucleotides ACGGAACTTTATTTGATGATAAAGGAGAAAAGCATGCCAAAAAAGGAGTCTGTATTTGGGAATGTATTGA CAGAATGCACAAGAGGAGTCCCATTTTCTTTAATTATTTATATTCACCATTGGAAATAGAGGCTCTAAAG CCCAATGTAAACGTCTCTAGCCTCAAGAAGTGGGATTACTACATAGAAGAGACCCTGTCCACAGGCCCTT CCTATGACTGGATGATGCTAACCCCCAAGCACTTCCCCTCCGAAGACTCTGACCTGGCTGGAGAAGCTGG GCCACGGAGCCAGAGGAGAACAGTGTGGCCATGCTATGATGATGTCAGCTGTACTCAGCCTGATGCTCTC ACCAGCCTTTTCAGTGAAATTGAAAAATTGGAGCACAAATTGAACCAAGCCCCTGAGAAGTGGCAGCAGC TGTGGGAAAGGGTAACCGTGGACCTTAAAGAAGAACCAAGAACAGATCGCTCCCAAAGACACCTGTCGAG ATCCCCAGGAATTGTGTCTACCAACCTACCTTCCTATCAGAAGAGGTCTCTGCTACATCTCCCAGACAGC AGCATGGGGGAGGAACAGAATTCCAGCATCTCCCCATCCAATGGAGTGGAGCGAAGAGCAGCCACGCTCT ATAGCCAGTATACATCCAAGAATGATGAAAACAGGTCCTTTGAGGGAACACTTTATAAAAGAGGGGCTTT GCTGAAAGGTTGGAAGCCCCGTTGGTTTGTTTTGGATGTAACAAAACATCAGCTGCGCTACTATGACTCA GGTGAGGACACAAGCTGTAAAGGCCACATTGATCTGGCTGAAGTAGAAATGGTCATCCCTGCTGGCCCCA GCATGGGAGCCCCAAAGCACACAAGTGACAAGGCTTTCTTTGATCTCAAGACCAGCAAACGTGTGTATAA CTTCTGCGCCCAGGATGGACAGAGTGCCCAGCAATGGATGGACAAGATCCAGAGTTGTATCTCTGATGCC TGATGCCCATGGTCAACCCACGCAGAAGAAACAGAAGAACTCATGCTGCCAGATAGATAGAAAAAGAAGC ATGGATCCTTGAGGAGCTGACAACAAGTTATCCCAGGGCCTGAGGTTCTCCTGCCCAGTCCCCTCTTGCA GGGGTTGCTATATCTACTTAACCTGAATAGGTGTTTCACACAGGTCTGGTCAACAGCCCCATGCACTCCC TGTATCTTGCACTAAATTTTTCTAACAGGGTCTTAGTGGTTAATGATCAGAAGATGTCTCCTGAGCCAAC TGTGAACCTCACCCAGGCAAAATGGCTACCACCTACTTGGGTCCTTCTTCATGAAAGCTATAGATCCTTT TTTGTTCTCTGAGGTCATAATTTCCTCGGAGACCTGTTTAACAAGCAAAAATCAAAACCCTCCAAGATTG TCTCATATTCTACCTGGACTAGGTTTCCTATGAGAGACATCTACTTGTAATGCCTGACCTTTGAGATGCT CAGTTCTCTGGTGCTGCCAAAAGATGCTTCCATGGTCCGTGCTCTGCCAGTGGGGTTCACAACAAGAGAC GTCATTGTTCAGTAGCAGGCAAAGAGGGAGCACACAGCATTATTCTGATGGAAAAAGATTATCCAGGGAA TGGTACAACAATGACCAGCCCAATGCAGGAAAACACTACTTCCAAAACACTGAATTCTCTAGACCAGAGG TGCTCTGAGGATCCAGGGCCTTGTGTTCTTATGTATCTTCTGCTTCCTGACAGCTTCTTTTTCAAAATAA CATGCAAAAAAAGCTGAATGCACTAACTCACAAAACAAACACTTGCACTGAATTCCCAATGAAGTGAAGA TGTTGGAAAGACAGAGGCCAGCTATTTAGGACCATACGCACCTGTGACAAGGGCTGTGTTGACCACAGTC ACACTGTGGCATGACTGGATACCCAAACTACACTTCTACACATGAAAAGTAAGAACTGTCTTTAGATTTT CTTTACTTTGATAACTTGTGATTGTTTAGCTTAAGACCCAAGAAATGCTGTTTGCTCATGGTAAACAGAA ACAGCATCTTCGCTACAACCACTGACACCAGCTGGCGTCATAGGTAGCTAGATCATTGCATTTGTTTTGA AATGTTAATATGTTAAATACTAAACTAATATTTCAAAAATGTGTATATATGATTTCTATATCCTTGTTTT TCAGATAGCCTGCTTATAATTTAATATAAATTAACTGATGCATTCATAAGATTTCAATAATGAAATGGTT CCCTTTTAAAAAATAAAAATACTTTGTAGATTAAAAATAAATCAGAATTTCAAATTTAAAATTGTCCACA CACTAGGAAATAGAACTGTGTTAATATATAAGAAATCTGGGGATAATTAAGAATGAAGGACTTTTCTATC ATTTCCATTTTATAAATTGCCACCTGTGAAAATGGTTTTTGCACATTATTTGTATTTTTCTTTGTATATG AAATAATTTTTTGTACTTTGTAAAATATGGAGCCCATTGTACCTTCAGCTATTTGAGACTATACACAGTG CTTCTTTTGTAACTGGATTACTTTAACTTTCGTGAAGGCATTACATTGCCTCACATTCACTAACCACCTT GAATTAAATTTATTTCTTAAGAAAAAGCAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC027492 unedited CACAATTTGTAATACGATCTCACTATANNGGCGGCCGCGNAATTCGGCACGAGGGGTTCA CAACCAGTATCCAACTGAGTTTGAATTCAATCTCTATTACTTAAAGTTCTTGGCTTTCCA CTATGTGTCTAATCGCTTTAAAACATTTCTCCTGGATTCAGACTATGAAAGATTAGAGCA CGGAACTTTATTTGATGATAAAGGAGAAAAGCATGCCAAAAAAGGAGTCTGTATTTGGGA ATGTATTGACAGAATGCACAAGAGGAGTCCCATTTTCTTTAATTATTTATATTCACCATT GGAAATAGAGGCTCTAAAGCCCAATGTAAACGTCTCTAGCCTCAAGAAGTGGGATTACTA CATAGAAGAGACCCTGTCCACAGGCCCTTCCTATGACTGGATGATGCTAACCCCCAAGCA CTTCCCCTCCGAAGACTCTGACCTGGCTGGAGAAGCTGGGCCACGGAGCCAGAGGAGAAC AGTGTGGCCATGCTATGATGATGTCAGCTGTACTCAGCCTGATGCTCTCACCAGCCTTTT CAGTGAAATTGAAAAATTGGAGCACAAATTGAACCAAGCCCCTGAGAAGTGGCAGCAGCT GTGGGAAAGGGTAACCGTGGACCTTAAAGAAGAACCAAGAACAGATCGCTCCCAAAGACA CCTGTCGAGATCCCCAGGAATTGTGTCTACCAACCTACCCTTCCTATCAGAAGAGTCTCT GCTACATCTCCCAGACAGCAGCATGGNGGGAGGAACAGAATTCCAGCATCTCCCCATCCA ATGGAGTGGAGCGAAGAGCAGCCACGCTCTATAGCCAGTATACATCCCACGAATGATGAA AACAAGTCCTTTGAGGAACACTTTATAAAAGAGGGGCTTTGCTGAAAAGTTGGAAGCCCC CTTGTTTTGTTTTGGATAGTACAAACATCAGCTGGCCTCCTTTACCTCAGTGAAGCACCA GCTGTAAGCGCCACTGACCTGCCTGAGAN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC027492 unedited ACGTTGAAAAAGCTTCTTTACGATATCGAAAAACCTTTTTTTTTTTTGCTTTTTCTTAAA AAATAAATTTAATTCAAGGTGGTCAGTGAATGTGAGGCAATGTAATGCCTTCACGAAAGT TAAAGTAATCCAGTTACAAAAGAAGCACTGTGTATAGTCTCAAATAGCTGAAGGTACAAT GGGCTCCATATTTTACAAAGTACTAAATAAGATTTCATATACAAAGAAAAATACAAATAA TGTGCAAAAACCATTTTCACAGGTGGCAATTTATAAAATGGAAATGATAGAAAAGTCCTT CATTCTTAATTATCCCCAAATTTCTTATATATTAACACAGTTCTATTTCCTAGTGTGTGG ACAATTTTAAATTTGAAATTCTGATTTATTTTTAATCTACAAAGTATTTTTATTTTTTAA AAGGGAACCATTTCATTATTGAAATCTTATGAATGCATCAGTTAATTTATATTAAATTAT AAGCAGGCTATCTGAAAAACAAGGATATAGAAATCATATATACACATTTTTGAAATATTA GTTTAGTATTTAACATATTAACATTTCAAAACAAATGCAATGATCTAGCTACCTATGACG CCAGCTGGTGTCAGTGGTTGTAGCGAAGATGCTGTTTCTGTTTACCATGAGCAAACAGCA TTTCTTGGGTCTTAAGCTAAACAATCACAAGTTATCAAAGTTAAGAAAATCTAAAGACAG TTCTTACTTTTCATGTGTAGAAGTGTANTTTGGGTATCCAGTCATGCCACAGTGTGACTG TGGTCACACAGCCCTTGTCACAGTGCGTATGGGCCTAATAGCTGGCCTCTGTCTTTCAAC ATCTTCCTTCATTGGAAATCAGTGCAGTGTTTGTTTGGGAGTAATGCATCACCTTTTTGC ATGTATTTGAAAAGAGCTGCGGGACAAAGAACTAAAACC
Restriction Sites	NotI-NotI
ACCN	BC027492
Insert Size	2900 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC027492.1
RefSeq Size	2633 bp
RefSeq ORF	2633 bp
Locus ID	81846
Domains	PH
Gene Summary	This gene encodes a pseudophosphatase and member of the myotubularin-related protein family. This gene maps within the CMT4B2 candidate region of chromosome 11p15 and mutations in this gene have been associated with Charcot-Marie-Tooth Disease, type 4B2. [provided by RefSeq, Jul 2008]

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