TBRG1 (BC032312) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FLJ14621; FLJ25020; FLJ90113; MGC129890; NIAM; nuclear interactor of ARF and MDM2; TB-5; transforming growth factor beta regulator 1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC032312, the custom clone sequence may differ by one or more nucleotides ATGAGCCTGCTGGACGGCCTCGCTTCCTCGCCGCGGGCTCCGCTGCAGTCCAGCAAGGCCAGGATGAAAA AGCTCCCGAAGAAGAGCCAGAATGAGAAGTACCGGCTGAAGTACCTGCGGCTGCGCAAAGCGGCCAAGGC CACGGTGTTTGTGAGTCTGACCCACGTTCAGCCGGTCCCCTCCTGGAGACTCCCTCACAAAATCTTTCCC CAAGCTGTTCCCCCTTCCCAGTGTGACAGTATTAATACAGTGTTGGCTTTTCCGTATACGTTACTACTTG TTAGCCTTGTGTCCTTGGACAAATTACGTAATTTCTCTGTGTCCTCATCTGTAAATGAGCTAGTAACTGT CTTGCAGAATAATGAATATTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC032312 unedited TTTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGCGGAAGTGCTGGG AGGCGCCGGGAGCCCGTTCGGTTGCGGGTGTCTCTGGCCCTGCGGTCAGCCCTGGGAACG TCCCGGAGAGCTAGATTCCTAGAGGCCCGATTCCGCTAGCCCGGAACAGACAAAGCCAGC GCTCCCGCCCGCTCCCCGACTTAGGATCCGATGCCGGCAGCGTCCTGGGGCCCCCGTAGC GGGGCTGGACCATGAGCCTGCTGGACGGCCTCGCTTCCTCGCCGCGGGCTCCGCTGCAGT CCAGCAAGGCCAGGATGAAAAAGCTCCCGAAGAAGAGCCAGAATGAGAAGTACCGGCTGA AGTACCTGCGGCTGCGCAAAGCGGCCAAGGCCACGGTGTTTGTGAGTCTGACCCACGTTC AGCCGGTCCCCTCCTGGAGACTCCCTCACAAAATCTTTCCCCAAGCTGTTCCCCCTTCCC AGTGTGACAGTATTAATACAGTGTTGGCTTTTCCGTATACGTTACTACTTGTTAGCCTTG TGTCCTTGGACAAATTACGTAATTTCTCTGTGTCCTCATCTGTAAATGAGCTAGTAACTG TCTTGCAGAATAATGAATATTAAATGAGTAAAGAAAAGTGCTTGGTGCGTAAAAGACATA CCAATAAATTNTAGTCATCGTTGTTACTGCAGGACTCTAGGAAATGTGTTTGATTCTCGA GATACAGCCCTGAGAATAGGCATTATTTGTAAAAGAGAACACTNGAGCCCAGAGAGATGA TTTGCCCCAGGGTACTGTGTCAGAGCCCTGACTCCAACNTTAGATAACCTGGATCCGTTT TATGACTTCCAGTACACAGACTATTTTCTGAGCCCTCCAATCCCAT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC032312 unedited AATCACTTGNACCGCGCCGCTTTCTANGATCGAGTTTTTTTTTTTTTTTTTTGTAAATGA TGACTCAATCTATCTCCCAAGCACCAATCCTGAAGGGAATGCTGAATCTGAATGCCCCCA TAGGTGGAACTTCAAACTCTCAATGTTCAAAACAACATCGCTTTTTCTTCACAAAATCTT CATTATGTATTTGATGGAACCAATATCCTTGAAATAACATAAATTCCAAGCTTTAGGTAC CTTTGACGTATAGGGGATAAAAGAAAGGGAACAGTCAATCAGATGCTAAGTTCTCTTAGT TTTACTTTTCTTGTTTCTCTCCAGTGCAAATAGCAAGCAATACACATAGCAAGTGCTGAA TAAGTGCTTTTGGAATGTGTATTCTAATTTCTGAACCTTTCCTTATGCTTCCAAAAATGC TGAGAGACGTTCAAGCTTTTGGTAGAGAGAAGAGATGAACTGTTGACTTGGATGTGGGGG GACATTTGAGGACTCCACCCCTATTTCCAAGCACTGAGGCAAGCCAAACTATTCTAATAT AAAGTTATGGCTGGCAAGCTATATTAAGTTTATTCACTCAATAAATCCAGTGCTTCTACA ATAAAAGAAAGGAAGAAACACTTGGAACACTCAAAGGTTAGAGGGGGGAAATTACTGACA GTTTCATGTACCTCTTTGGGCAAAGGGTCAGTTATGGGGATTTGAGGTGCTCAGAAAATA GTCTGTGTACTGGAAGTCATAAAACGGAATCAGGTTTATCTAAGTTGGAGTCAGGGCTCT GCACAGTACCCTTGGGCAAATCATCTCTCTGGGCTTCAGTGTTCTCATTTTACAAATATG CCCTATTCTCAGGNCTGTATCTCGAGAATCAAACACATTTCCTAGAGTCCCTGCAGTACA ACGATGACTAAAATTTATTGGTATGTCA
Restriction Sites	NotI-NotI
ACCN	BC032312
Insert Size	1400 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC032312.1, AAH32312.1
RefSeq Size	1498 bp
RefSeq ORF	372 bp
Locus ID	84897
Gene Summary	Acts as a growth inhibitor. Can activate p53/TP53, causes G1 arrest and collaborates with CDKN2A to restrict proliferation, but does not require either protein to inhibit DNA synthesis. Redistributes CDKN2A into the nucleoplasm. Involved in maintaining chromosomal stability.[UniProtKB/Swiss-Prot Function]

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