LAS1L (AK054695) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	dJ475B7.2; Las1-like
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK054695, the custom clone sequence may differ by one or more nucleotides CTACAGCTCCTCAACTCCAGTGACTTTCATTCTCCACTACACTCAGCAACCCATTTCCATCATTCGGAGC TATTCCACCTCTGAAAGCTCACATCCCAGCATCCCACTCGGTGACCACCACTTCTCGTCCTTCTAGTTCT CACCCTTGCATCCCATGACATCTGCTCTTTGCCTGCATCCAGATCTCCATCCCTCCATTTCCTGCATCCC TTGACCTTGCTTCCTCCCTATCCTGTCTGTGGTTGCTCAGTTCGGCCACTCATCATCAGCACCTTCAGCT TTGCCCATCCTTTGGCCATATCTGACTTGCAAACCCCTGAGCTGGAATTGATCCTCCCCATTCTGCTTCC CCTGCTCCTCAGCCCGGGTAGCTGAGCTCTGCTGGAGAAAACCACATAGCCCTGCGGATTGCTGCCACTG CAGAGCCCCTCCCCCTCTCCCATTTCACCCTAGCTTCCTCGAGCACATGGAGGCATCCGGCAGGGCCCCC TCGACCTGAGGTCCAGCCCTCGTGAACTGATCTGCGGCTGCACTCAACCTTCTCGCCAGTCTCAGGATGA GGTGGCCTTGCCCCACCTCAAAGCCTGCTCCTCCACCTGTGCTCTGGAGCCACCTCTGCCAGCATCGCTG GGGCCTCACCCCAGCCTCTACCTTGCTCTGCTGGCTCTTGCTCTTCAACCTAGGAACGTGCCTGAGCTTT TCTCATCTTAAACAAAACAACAATAACAGCAACACAAGCAAAATCTCCTTTGACCCTGCATCCCTCTGTT GGGTTATCATCAGCTTATCATTCCCACCCTTCCCATCAAAACATCTCAAAAGAGTAGTCTACACTCAACA CTCTCCATTTCCTCACTACCCTCTTACTCCTCAACCTGCTGCAATTTGACATTCACCCCCAGAGTTGCTT TGACCTCCTAATTACCAAATCAGTGGGCCCTTGTCAGTCTCTGTGTCTGCTGCATTGATGCTGTTTACCA CCCCCTCCTCAAAAAATGCCTGTGCTTATTACTAGTTCCTATAAAGTGCTGAAACCCAAATCTGTCTAGC CCTGGCCCCTCCCCCAAGATTCAGCCTCCTGTACTCGACTACATGTCAGACATCTCCCCTTGGATGGCCC ACAGGCACCTTACTGTGACCAAAAGAGAATTGGTCGTCTCAGTCCCCATCTCAACTCTAAAGTGATCTTC CTCCTCTGTTCCCTATCTCACTTGGTGGCGGCACCATCTACCCTGTCCTGTTGACTCTCGAATACTTCCC AAATCTGAGGTTTTGTGTTGCCGTGTCTACTTCCCTTAGTGTCTCCTTCCCTCTATCAGACTAAGCTTTT CAAGGGCAAGGACTCTACATCAGCTGTCCGTGCCCTAGCACACCACACAAGTCTAGGCACCAAACAGGCC TCAGTAAATGTCGTTGAATTGAACCGTGCTCTGCTCTCTCATTCCCAGCAGTGCTGTCCTTGGTGGGAGC TGACCCAGTGCCTGGGGAATGAATGAAGTGGGAGTCTGGATGCCAAGACCCTCTTAGTCCCTGATCCCCC TATTGAGTCCAAAAGTCTTTCTTTAACCCTGATGGCATTTGGGACACAGAGGTATTTGTAAGGTTTTAAA AGTGGCTTTGTAGACAAAGTCAGTCAAGGCAAGACTTCCAGGAGTTGGAACTGAACAGCCACATTGCCAG ATGTGTGCTCCAGACTAGAAGTGATGTGGATGGGTGGGCAATTGGGAGGAGAACCAAAAGGGCCTGGATG AGTCAGAGTTTCAAGAGGAGGCATTTGAACAATTCAAGCAGGAGAAAGAATAAAGCAAGTTTCAAAGTCC AGAAGGAACATGGCATGTGTATATAATGGGAGGGAGGGAGTGACAAGAGCAGCATGGAGAGGTTTGGATA GGTGGCCTTCAAGGTACTTTCCAATCCTAAGTGCCTGCAGAGTGGAAGCTGCTGGTCTCGGAGAAGTCTG GACCACAGAGATCTTCTCCCAACCCAAGCATACCAGAGGGATGCGACACATGCCGGCAGTAACAGCGGCT CACTTGGGTACGATTTCTTGTGCACAGATCCTGCCTTTCCCAGATAAGCAGTGAATCTAGTCCTAATCAC CCCGTTACACAGAGTGGAGAACAGAGGGGGTTTGCCCAGGTATTATGCTGCAAGTCAGAGCTGGGGCCAG AATCCAGATAGCCTGTCCTTAGTCCCATGCTCTTCTGTAATACTCCACTAGATTGTATTATTGCAGAAAG CATCTGTTAAACACAGAACTTGGCTTTTGTTTTCCCCAGAGAAAAAATAAAAGATACTATTGGAAATTAA GTGTCAGG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK054695 unedited GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCTCGTGAACTGATCT GCGGCTGCACTCAACCTTCTCGCCAGTCTCAGGATGAGGTGGCCTTGCCCCACCTCAAAG CCTGCTCCTCCACCTGTGCTCTGGAGCCACCTCTGCCAGCATCGCTGGGGCCTCACCCCA GCCTCTACCTTGCTCTGCTGGCTCTTGCTCTTCAACCTAGGAACGTGCCTGAGCTTTTCT CATCTTAAACAAAACAACAATAACAGCAACACAAGCAAAATCTCCTTTGACCCTGCATCC CTCTGTTGGGTTATCATCAGCTTATCATTCCCACCCTTCCCATCAAAACATCTCAAAAGA GTAGTCTACACTCAACACTCTCCATTTCCTCACTACCCTCTTACTCCTCAACCTGCTGCA ATTTGACATTCACCCCCAGAGTTGCTTTGACCTCCTAATTACCAAATCAGTGGGCCCTTG TCAGTCTCTGTGTCTGCTGCATTGATGCTGTTTACCACCCCCTCCTCAAAAAATGCCTGT GCTTATTACTAGTTCCTATAAAGTGCTGAAACCCAAATCTGTCTAGCCCTGGCCCCTCCC CCAAGATTCAGCCTCCTGTACTCGACTACATGTCAGACATCTCCCCTTGGATGGCCCACA GGCACCTTACTGTGACCAANAGAGAATTGTTCGTCTCAGTCCCCATCTCAACTCTAAAGT GATCTTCCTCCTCTGNTCCCTATCTCACTTGGTGGCGGCACCATCTACCCTGTCCTGGTG ACTCTCGAATACTTCCCAAATACTGAGTTTTTGTGTTGCCCGNGCCTACTTTCCCTTAGT GGCCTCCCTCCCCTCTATCAGACTAAGCTTTTCAAGGCCAAGGACTCTACATAAGCTGGT CCTGGCCCTAGCAC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK054695 unedited AGCTCTGGAACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCAATTCAA CGACATTTACTGAGGCCTGTTTGGTGCCTAGACTTGTGTGGTGTGCTAGGGCACGGACAG CTGATGTAGAGTCCTTGCCCTTGAAAAGCTTAGTCTGATAGAGGGAAGGAGACACTAAGG GAAGTAGACACGGCAACACAAAACCTCAGATTTGGGAAGTATTCGAGAGTCAACAGGACA GGGTAGATGGTGCCGCCACCAAGTGAGATAGGGAACAGAGGAGGAAGATCACTTTAGAGT TGAGATGGGGACTGAGACGACCAATTCTCTTTTGGTCACAGTAAGGTGCCTGTGGGCCAT CCAAGGGGAGATGTCTGACATGTAGTCGAGTACAGGAGGCTGAATCTTGGGGGAGGGGCC AGGGCTAGACAGATTTGGGTTTCAGCACTTTATAGGAACTAGTAATAAGCACAGGCATTT TTTGAGGAGGGGGTGGTAAACAGCATCAATGCAGCAGACACAGAGACTGACAAGGGCCCA CTGATTTGGTAATTAGGAGGTCAAAGCAACTCTGGGGGTGAATGTCAAATTGCAGCAGGT TGAGGAGTAAGAGGGTAGTGAGGAAATGGAGAGTGTTGAGTGTAGACTACTCTTTTGAGA TGTTTTGATGGGAAGGGTGGGAATGATAAGCTGATGATAACCCAACAGAGGGATGCAGGG TCAAAGGAGATTTTGCTTGGGTTGCTGTTATTGGTGTTTTGTTTAAGATGAGAAAAGCTC ANGCACGTTCCCTAGTTGAAGAAGCAGAGCCANCAAANCAGGTANNAGCTGGGGGTGAGC CCCANNCGATGCTGCANNAGTGGCTCCANACACAGGTGGAGGAGCANGCTTTGAGGTGGG GCAAGGCCACTCATCCTGAGACTGGCGAGAAGTTGAGTGCAGCCGCAGATCAGTCACGAG GCCC
Restriction Sites	NotI-NotI
ACCN	AK054695
Insert Size	900 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK054695.1
RefSeq Size	2318 bp
RefSeq ORF	2318 bp
Locus ID	81887
Gene Summary	Involved in the biogenesis of the 60S ribosomal subunit. Required for maturation of the 28S rRNA. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.