NOL10 (AK024000) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PQBP5
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK024000, the custom clone sequence may differ by one or more nucleotides ATGACTACTCAAAGTTCTGAAGTTTATAGGTTAAACTTAGAACAAGGACGATACCTGAATCCTCTACAAA CTGATGCTGCGGAGAATAATGTTTGTGACATAAATTCAGTGCATGGCTTGTTTGCCACAGGAACCATAGA GGGTAGAGTGGAGTGCTGGGACCCAAGAACTCGAAACAGAGTTGGCCTGTTAGACTGCGCCTTAAACAGT GTCACAGCAGATTCAGAGATAAACAGTTTACCAACAATCTCTGCTTTGAAATTTAATGGTGCCTTGACCA TGGCAGTTGGAACAACCACAGGGCAGGTTTTATTATATGACCTTCGATCTGATAAGCCATTGCTAGTTAA AGATCACCAGTATGGGCTGCCCATTAAGTCCGTTCATTTCCAGGATTCATTAGATCTGATTTTGTCTGCT GACTCTCGAATTGTCAAGATGTGGAATAAGAACTCCGGAAAAATATTTACTTCCTTGGAGCCAGAGCATG ACCTTAATGATGTTTGTCTCTACCCCAACTCAGGCATGCTTCTGACGGCCAATGAAACCCCCAAGATGGG CATCTATTACATTCCACCTTCCCTCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK024000 unedited TTCAAATTTTGTAATACGACTTACTATTAGGGCGGCCGCGAATTCGCACGAGGGCCGCAC GCCGCGGAGCAGGGGCTCGGAGGTCCCGGGATTACGGTGCTCGAGCACGCTGGTGGGAAA GGACCCGGGACTTGAACAGTGTTGTGCGGCGCCATGCAGGTCTCCAGCCTCAATGAGGTG AAGATTTACAGCCTCAGCTGCGGCAAGTCCCTTCCTGAGTGGCTTTCTGATAGGAAGAAG AGAGCGCTACAGAAGAAAGATGTAGATGTCCGTAGGAGAATTGAACTTATTCAGGACTTT GAAATGCCTACTGTGTGTACCACTATTAAGGTGTCAAAAGATGGACAGTACATTTTAGCA ACTGGAACATATAAACCTCGGGTTCGATGTTATGACACCTATCAATTATCCTTGAAGTTT GAAAGGTGTTTAGATTCAGAAGTTGTCACCTTTGAAATTTTGTCTGATGACTACTCAAAG ATTGTCTTCTTACATAATGATAGATACATTGAATTTCATTCGCAATCAGGTTTTTACTAC AAAACCAGAATACCAAAGTTTGGGAGAGATTTCTCTTACCACTATCCATCCTGTGACTTG TACTTTGTTGGTGCAAGTTCTGAAGTTTATAGGTTAAACTTAGAACAAGGACGATACCTG AATCCTCTACAAACTGATGCTGCGGAGAATAATGTTTGTGACATAAATTCAGTGCATGGC TTGTTTGCCACAGGAACCATANAGGGTANNAGTGGAGTGCTGGGACCCAAGAACTCGNNA ACAGAGTTGGCCTGTTAGACTGCGCCTTTAACAGTGTCACAGCAGATTCAGAGATAAACA GTTTACCAACAATCTCTGCTTTGAAATTAATGGTGCCTTGACCATGGCAGTTGGNAACAC ACAGGNCANNGTTTGGGTNCCTGCTCTCGNGGGTGTCCTTCTTANACAACTGCCGAGAAT ANNAGAGATCCCGAAGCCGTCTTGAT
Restriction Sites	NotI-NotI
ACCN	AK024000
Insert Size	2440 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK024000.1, BAB14765.1
RefSeq Size	1418 bp
RefSeq ORF	588 bp
Locus ID	79954

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