CHAC2 (BC025376) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ChaC, cation transport regulator-like 2; ChaC, cation transport regulator homolog 2 (E. coli)
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC025376, the custom clone sequence may differ by one or more nucleotides ATGTGGGTTTTTGGTTACGGGTCCCTGATCTGGAAGGTGGATTTCCCCTATCAGGACAAGCTGGTCGGAT ACATCACCAACTACAGCAGGCGCTTCTGGCAGGGCAGCACGGACCACCGCGGGGTCCCCGGCAAGCCTGG AAGAGTTGTGACTCTTGTTGAAGATCCTGCGGGATGTGTATGGGGTGTTGCTTACAGATTGCCAGTAGGA AAGGAAGAAGAAGTAAAAGCATACCTTGACTTCAGAGAAAAAGGAGGCTACAGAACCACAACAGTCATTT TTTATCCAAAAGATCCCACAACAAAACCATTCAGTGTATTGCTATATATTGGAACATGTGATAATCCTGA TTATCTTGGTCCTGCACCTCTGGAAGACATTGCTGAACAAATTTTTAATGCAGCTGGTCCAAGTGGAAGA AATACAGAATATCTTTTTGAACTTGCAAATTCTATTAGGAACCTTGTGCCAGAAGAAGCAGATGAGCATC TTTTCGCTTTGGAAAAATTAGTAAAGGAACGTTTAGAAGGGAAACAGAACCTCAATTGCATATAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC025376 unedited TCAGAAATTTGNTATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGGCGACA GCTAGGGTTCACGGCCACTGGGGCAGAGGAGCCGCGAGAAGAGTGGGTTTTTGGTTACGG GTCCCTGATCTGGAAGGTGGATTTCCCCTATCAGGACAAGCTGGTCGGATACATCACCAA CTACAGCAGGCGCTTCTGGCAGGGCAGCACGGACCACCGCGGGGTCCCCGGCAAGCCTGG AAGAGTTGTGACTCTTGTTGAAGATCCTGCGGGATGTGTATGGGGTGTTGCTTACAGATT GCCAGTAGGAAAGGAAGAAGAAGTAAAAGCATACCTTGACTTCAGAGAAAAAGGAGGCTA CAGAACCACAACAGTCATTTTTTATCCAAAAGATCCCACAACAAAACCATTCAGTGTATT GCTATATATTGGAACATGTGATAATCCTGATTATCTTGGTCCTGCACCTCTGGAAGACAT TGCTGAACAAATTTTTAATGCAGCTGGTCCAAGTGGAAGAAATACAGAATATCTTTTTGA ACTTGCAAATTCTATTAGGAACCTTGTGCCAGAAGAAGCAGATGAGCATCTTTTCGCTTT GGAAAAATTAGTAAAGGAACGTTTAGAAGGGAAACAGAACCTCAATTGCATATAATTTAG TCTTCAGAGAATTAACTTCAGTGCACAATGACAATATGATTTGGAAATACGTTTACTTAA AGATCTTATTTTTAATGTAGTGAGGATATTATTTAAACTTTTATTTTAACTGGGAATGTN NCTGAACACATATTTAAAATATTGNGATACAGTGAAAGAANAATTCANATTTTAATAACA TAAAGATTTCCTAACTCTATGTTATTGAACACTTACTCACTAGNAGTGAGTTCTTTAGAA AATACAGTGAAGGACTCAGTTCAGTCTTGTTTTATCAAAGTGATATCATCCTGTTTACAT NCCATACTATTTGAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC025376 unedited AGTTGGCCATTCTTTATTAAGGATTAGTACAGAACACTATAATTCCAAGNAAATTTTCTT TTTTTAAAAATGCCAATTTTTGGCTAAAAACTCAAAAGATTTCATTTAATACTTGAATAA CAAGGAAATTCACAATTAAGGCTGAAATACTGTTCATATTATTGTGTATTTCTAAGCATG TTATTTTAAGCTTGTGTATTTTAAACTAAGATAACTGTAATTGTATAGGAATATATTGAA GGCATAACCTTATATTTATTGGAATTTTGGTTTAATTGTTTAGAATTTCAAAATAGTATT GGGATGTGAAACAGGATGATTATCACTCTGATAAAAACAAGACTGAACTGAGTCCTTCAC TGTATTTTTCTAAAGAACTCACTTCTAGTGAGTAAGTGTTCAATAACATAAAGTTAGGAA ATCTTTATGTTATTAAAATTTGAATTTTTCTTTCACTGTATCCCAATATTTTAAATATGT GTTTCAGGACATTTCCAGTTTAAATAAAAGTTTAAATAATATCCTCCCTACTTTAAAAAT AAGATCTTTAAGTAAACGTATTTCCAAATCATATTGTCAATGTGCACTGAAGTTAATTCT CTGAAAACTAAAATATATGCAATTGAAGTTCTGTTTCCCTTCTAACCGTCCCTTTACTAT TTTTTCCAAACCAAAAAAAGCCTCATCTGCTTTCTTCTGGCACCAAGTATCCAAATAGAA TTTTGCAAGTTCAAAAGAATATTCTGTTTTTCTCCCCTTTGGACAACTGCATTAAAATTT TTTCAACAAGGTCTTCCAAAAGTGCCGGAACCACAAAAACAAGATATTCAATTTTCCCAA TTTTACCCATCCCTGAATGGTTTTGTTGGGGAATTTTTTGAATAAAAAGAAGTTAGTGGA TCGAGACCCACTTTCTTTAAAACAGATAGCTCTTAATTTATTGTCTTTCAAAGGGCGGCG TGGCACCCG
Restriction Sites	NotI-NotI
ACCN	BC025376
Insert Size	1250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC025376.1, AAH25376.1
RefSeq Size	1311 bp
RefSeq ORF	1311 bp
Locus ID	494143
Domains	ChaC
Gene Summary	The protein encoded by this gene is a gamma-glutamyl cyclotransferase that catalyzes the conversion of glutathione to 5-oxoproline and cysteinylglycine. It is thought that this gene is upregulated in response to endoplasmic reticulum stress and that the glutathione depletion enhances apoptosis. [provided by RefSeq, Sep 2016]

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