MMAA (NM_172250) Human Untagged Clone
CAT＃: SC101283
MMAA (untagged)-Human methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), nuclear gene encoding mitochondrial protein
CNY 7220.00
| Cited in 1 publication. |
Product images
CNY 1999.00
CNY 2700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | cblA |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_172250, the custom clone sequence may differ by one or more nucleotides
ATGCCCATGCTGCTACCACATCCTCACCAGCATTTCCTAAAAGGCCTTTTAAGAGCACCTTTCCGATGTT ACCACTTCATCTTTCACTCAAGTACTCATCTCGGATCAGGAATCCCATGTGCTCAGCCGTTTAATTCTCT TGGACTCCATTGTACAAAGTGGATGCTGCTGTCAGATGGCTTAAAGAGAAAATTATGTGTACAAACAACC TTAAAGGACCACACAGAAGGACTTTCTGATAAAGAGCAAAGATTTGTGGATAAACTTTATACTGGTTTAA TCCAAGGGCAAAGGGCCTGTTTAGCAGAGGCCATAACTCTTGTAGAATCAACTCACAGCAGGAAAAAGGA GTTAGCCCAGGTGCTTCTTCAGAAAGTATTACTTTACCACAGAGAACAAGAACAATCAAATAAAGGAAAA CCACTAGCATTTCGAGTAGGATTGTCTGGGCCCCCTGGTGCTGGAAAATCAACATTTATAGAATATTTTG GAAAAATGCTTACTGAGAGAGGGCACAAATTATCTGTGCTAGCTGTGGACCCTTCTTCTTGTACTAGTGG TGGATCACTCTTAGGTGATAAAACCCGAATGACTGAGTTATCAAGAGATATGAATGCATACATCAGGCCA TCTCCTACTAGAGGAACTTTAGGAGGCGTGACAAGGACCACAAATGAAGCTATTCTGTTGTGTGAAGGAG CGGGATATGACATAATTCTTATTGAAACCGTTGGTGTGGGTCAGTCGGAGTTTGCTGTTGCTGACATGGT TGACATGTTTGTTTTACTACTGCCACCAGCAGGAGGAGATGAGCTGCAGGGTATCAAAAGGGGTATAATC GAGATGGCAGATCTGGTAGCTGTAACTAAATCTGATGGAGACTTGATTGTGCCAGCTCGAAGGATACAAG CGGAATATGTGAGTGCACTGAAATTACTCCGCAAACGTTCACAAGTCTGGAAACCAAAGGTAATTCGTAT TTCTGCCCGAAGTGGAGAGGGGATCTCTGAAATGTGGGATAAAATGAAAGATTTCCAGGACCTAATGCTT GCCAGTGGGGAGCTGACTGCCAAACGACGGAAGCAACAGAAAGTTTGGATGTGGAATCTCATTCAGGAAA GTGTGTTAGAGCATTTCAGGACCCACCCCACAGTCCGGGAACAGATTCCACTTCTGGAACAAAAGGTTCT CATTGGGGCCCTGTCCCCAGGACTAGCAGCAGACTTCTTGTTAAAAGCTTTTAAAAGCAGAGACTAA >OriGene 5' read for NM_172250 unedited
TGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGTGGCACTTCCGTGGCTTCG GGCGGGGAGGTCACAATCACATTGAGCCAAAACGCATCCAGTGTTTTCTCCAGTTACAAA TAAAACGAATATGCCCATGCTGCTACCACATCCTCACCAGCATTTCCTAAAAGGCCTTTT AAGAGCACCTTTCCGATGTTACCACTTCATCTTTCACTCAAGTACTCATCTCGGATCAGG AATCCCATGTGCTCAGCCGTTTAATTCTCTTGGACTCCATTGTACAAAGTGGATGCTGCT GTCAGATGGCTTAAAGAGAAAATTATGTGTACAAACAACCTTAAAGGACCACACAGAAGG ACTTTCTGATAAAGAGCAAAGATTTGTGGATAAACTTTATACTGGTTTAATCCAAGGGCA AAGGGCCTGTTTAGCAGAGGCCATAACTCTTGTAGAATCAACTCACAGCAGGAAAAAGGA GTTAGCCCAGGTGCTTCTTCAGAAAGTATTACTTTACCACAGAGAACAAGAACAATCAAA TAAAGGAAAACCACTAGCATTTCGAGTAGGATTGTCTGGGCCCCCTGGTGCTGGAAAATC AACATTTATAGAATATTTTGGAAAAATGCTTACTGAGAGAGGGCACAAATTATCTGTGCT AGCTGTGGACCCTTCTTCTTGTACTAGTGGTGGATCACTCTTAGGTGATAAAACCCGAAT GACTGAGTTATCAAGAGATATGAATGCATACATCAGGCCATCTCCTACTAGAGGAACTTN TAGAGGCGTGACAGGGACCACAAATGAAGCTATTCTGTTGTGTGAAGGAGCGGGATATGA CATAATTCTTATTGAAACCGTTGGGTGTGGGTCAGTCGGGAGTTGC >OriGene 3' read for NM_172250 unedited
TATGGGATGGCAACTTCCAGGTCCAGGNAAAGCACTGGGGNAGGGTCACAGGGATGCCAC CCGTTATCTGTTCAGGAAACAGCTATGACCGGGGGCGCAATCTAAAGTCGAGTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTGCCGTAGAGGTTCAACTACATAAATTATCACCCGTAT GTTGTTCGTTAGCTGTTGTCTTCAGTGTACACTGGGCAATAGCTATAAAAGCTTAAGGAA ATGGCCGGGTGATCGAGTGATTCTTAAAAAAAAATGCCTTGTTAGGTTGTATTGGGATAG CTACTGGCAACTATTGAAATAACTGCCCTCATTTTAATGAGATTTGATATTGTTTTAGCA AAATGTCTGTCAGTTTTGGAGCTCAACACATTTTTTTCCTATCTACAGAAGTTAATGGGA ATCATATTCTTGACAAGGCAAAGTGAACCTATGAGATTTTTTTCAAAATTATCTTTTTAA AATGGACACTGTGACTCGTTTAATCCTCATAACAGTTCTAAGAGATAACTCCCACTTCCT GGAAAATATATATTTACCACCCGGGATCAGCCCCCAATTCCGCAAAACCACCCCCCACCG CGGCCCCTTAAAATTTCCCCAACAAAAAACCCCCCATTACGCGCACGGGCCCGAACCCCC AAACAAACCACGGGGCCCTCCCCCCGCAAAACCCCCCAGGGCACGCCCCCCCCCCCCTAC AAAACCCCCCAGAACCCCCCCCATAAAACCCCTATCAAAATCCCCCGACTCCCCCCCCAA ATTAGACCCCCCCGCCCCGCCCAGCTCCATAAAAATTCCCGAAAAAAAACCCCCCAAACC CCCCGCCAATATATATCACCCATTAGCCCCCCCCCCCCCCCCCCCCCATT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_172250 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_172250.1, NP_758454.1 |
| RefSeq Size | 1473 bp |
| RefSeq ORF | 1257 bp |
| Locus ID | 166785 |
| UniProt ID | Q8IVH4 |
| Protein Families | Druggable Genome |
| Gene Summary | The protein encoded by this gene is involved in the translocation of cobalamin into the mitochondrion, where it is used in the final steps of adenosylcobalamin synthesis. Adenosylcobalamin is a coenzyme required for the activity of methylmalonyl-CoA mutase. Defects in this gene are a cause of methylmalonic aciduria. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Structures of the Human GTPase MMAA and Vitamin B12-dependent Methylmalonyl-CoA Mutase and Insight into Their Complex Formation
,D. Sean Froese, Grazyna Kochan, João R. C. Muniz, Xuchu Wu, Carina Gileadi, Emelie Ugochukwu, Ewelina Krysztofinska, Roy A. Gravel, Udo Oppermann, and Wyatt W. Yue,
J. Biol. Chem., Dec 2010; 285: 38204 - 38213
[MMAA]
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Other Versions
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| RC223205L1 | Lenti ORF clone of Human methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
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| RC223205L2 | Lenti ORF clone of Human methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), nuclear gene encoding mitochondrial protein, mGFP tagged |
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| RC223205L3 | Lenti ORF clone of Human methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
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| RC223205L4 | Lenti ORF clone of Human methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), nuclear gene encoding mitochondrial protein, mGFP tagged |
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| RG223205 | MMAA (tGFP-tagged) - Human methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), nuclear gene encoding mitochondrial protein |
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