BRSK2 (NM_003957) Human Untagged Clone
CAT＃: SC101068
BRSK2 (untagged)-Human BR serine/threonine kinase 2 (BRSK2)
CNY 4904.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C11orf7; PEN11B; SAD1; SADA; STK29 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_003957 edited
ATGACATCGACGGGGAAGGACGGCGGCGCGCAGCACGCGCAGTATGTTGGGCCCTACCGG CTGGAGAAGACGCTGGGCAAGGGGCAGACAGGTCTGGTGAAGCTGGGGGTTCACTGCGTC ACCTGCCAGAAGGTGGCCATCAAGATCGTCAACCGTGAGAAGCTCAGCGAGTCGGTGCTG ATGAAGGTGGAGCGGGAGATCGCGATCCTGAAGCTCATTGAGCACCCCCACGTCCTAAAG CTGCACGACGTTTATGAAAACAAAAAATATTTGTACCTGGTGCTAGAACACGTGTCAGGT GGTGAGCTCTTCGACTACCTGGTGAAGAAGGGGAGGCTGACGCCTAAGGAGGCTCGGAAG TTCTTCCGGCAGATCATCTCTGCGCTGGACTTCTGCCACAGCCACTCCATATGCCACAGG GATCTGAAACCTGAAAACCTCCTGCTGGACGAGAAGAACAACATCCGCATCGCAGACTTT GGCATGGCGTCCCTGCAGGTTGGCGACAGCCTGTTGGAGACCAGCTGTGGGTCCCCCCAC TACGCCTGCCCCGAGGTGATCCGGGGGGAGAAGTATGACGGCCGGAAGGCGGACGTGTGG AGCTGCGGCGTCATCCTGTTCGCCTTGCTGGTGGGGGCTCTGCCCTTCGACGATGACAAC TTGCGACAGCTGCTGGAGAAGGTGAAGCGGGGCGTGTTCCACATGCCGCACTTTATCCCG CCCGACTGCCAGAGTCTGCTACGGGGCATGATCGAGGTGGACGCCGCACGCCGCCTCACG CTAGAGCACATTCAGAAACACATATGGTATATAGGGGGCAAGAATGAGCCCGAACCAGAG CAGCCCATTCCTCGCAAGGTGCAGATCCGCTCGCTGCCCAGCCTGGAGGACATCGACCCC GACGTGCTGGACAGCATGCACTCACTGGGCTGCTTCCGAGACCGCAACAAGCTGCTGCAG GACCTGCTGTCCGAGGAGGAGAACCAGGAGAAGATGATTTACTTCCTCCTCCTGGACCGG AAAGAAAGGTACCCGAGCCAGGAGGATGAGGACCTGCCCCCCCGGAACGAGATAGACCCT CCCCGGAAGCGTGTGGACTCCCCGATGCTGAACCGGCACGGCAAGCGGCGGCCAGAACGC AAGTCCATGGAGGTGCTCAGCGTGACGGACGGCGGCTCCCCGGTGCCTGCGCGGCGGGCC ATTGAGATGGCCCAGCACGGCCAGAGGTCTCGGTCCATCAGCGGTGCCTCCTCAGGCCTT TCCACCAGCCCACTCAGCAGCCCCCGGGTGACCCCTCACCCCTCACCAAGGGGCAGTCCC CTCCCCACCCCCAAGGGGACACCTGTCCACACGCCAAAGGAGAGCCCGGCTGGCACGCCC AACCCCACGCCCCCGTCCAGCCCCAGCGTCGGAGGGGTGCCCTGGAGGGCGCGGCTCAAC TCCATCAAGAACAGCTTTCTGGGCTCACCCCGCTTCCACCGCCGGAAACTGCAAGTTCCG ACGCCGGAGGAGATGTCCAACCTGACACCAGAGTCGTCCCCAGAGCTGGCGAAGAAGTCC TGGTTTGGGAACTTCATCAGCCTGGAGAAGGAGGAGCAGATCTTCGTGGTCATCAAAGAC AAACCTCTGAGCTCCATCAAGGCTGACATCGTGCACGCCTTCCTGTCGATTCCCAGTCTC AGCCACAGCGTCATCTCCCAAACGAGCTTCCGGGCCGAGTACAAGGCCACGGGGGGGCCA GCCGTGTTCCAGAAGCCGGTCAAGTTCCAGGTTGATATCACCTACACGGAGGGTGGGGAG GCGCAGAAGGAGAACGGCATCTACTCCGTCACCTTCACCCTGCTCTCAGGCCCCAGCCGT CGCTTCAAGAGGGTGGTGGAGACCATCCAGGCCCAGCTGCTGAGCACACACGACCCGCCT GCGGCCCAGCACTTGTCAGACACCACTAACTGTATGGAAATGATGACGGGGCGGCTTTCC AAATGTGGAATTATCCCGAAAAGTTAA >OriGene 5' read for NM_003957 unedited
CGGCACGAGGCCGGGTCGGCGCGGACGGCACTCGGCGGACGCGGGCGGACGCTGGGCGGC CCCTCCCTGCCCGCGCGCCCGGGCGCCCCTGGCCGGCGCCGGGCCCCAGAGCGATGACAT CGACGGGGAAGGACGGCGGCGCGCAGCACGCGCAGTATGTTGGGCCCTACCGGCTGGAGA AGACGCTGGGCAAGGGGCAGACAGGTCTGGTGAAGCTGGGGGTTCACTGCGTCACCTGCC AGAAGGTGGCCATCAAGATCGTCAACCGTGAGAAGCTCAGCGAGTCGGTGCTGATGAAGG TGGAGCGGGAGATCGCGATCCTGAAGCTCATTGAGCACCCCCACGTCCTAAAGCTGCACG ACGTTTATGAAAACAAAAAATATTTGTACCTGGTGCTAGAACACGTGTCAGGTGGTGAGC TCTTCGACTACCTGGTGAAGAAGGGGAGGCTGACGCCTAAGGAGGCTCGGAAGTTCTTCC GGCAGATCATCTCTGCGCTGGACTTCTGCCACAGCCACTCCATATGCCACAGGGATCTGA AACCTGAAAACCTCCTGCTGGACGAGAAGAACAACATCCGCATCGCAGACTTTGGCATGG CGTCCCTGCAGGTTGGCGACAGCCTGTTGGAGACCAGCTGTGGGTCCCCCCACTACGCCT GCCCCGAGGTGATCCGGNGGGAGAAGTATGACGGCCCGAAGGCGNACGTGTGGAGCTGCG GCGTCATCCTGTTCGCCTTGCTGGTGGGGGCTCTGCCCTTCGACGATGACAACTTGCGAC AGCTGCTGGAGAAGGTGAGCGGNGCGTGTNCACATGCCGCACTTTATTCCGCCGACTGCC ANATCTGCTAGGGGCATGATCGAGGTGGAGCC >OriGene 3' read for NM_003957 unedited
TATGAACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTAAAAAAATAACTTGCAGGAATTTTTTAAAGTTTACAGTAG CTTGCCAGCCCCCCCGCGCCCCACCAGTTTTGTCCTTAAAGGGCGCCCTGGCCCCAGCCC CCCCACTGCCTGAAACCCCCAAACGGGAGGGGATGTGGCCCCCCCCCTTCCCTGAACAGA GGTGCGGAGAATCCATAAATTAAAACGGCAAATAAATAACACTGATCCCAGCCCAACGGC CTGGCCTGGTGGGAAGGCTCTGGTGCCACAGGGTGTCTTTGTTTCCACCTCAGCCAAAGA GGAGCGCCAAAACCCACAGCCCCCCCAAATGAAGCAAGGGAGGCCCGGGTGAAACCAAAG GACCGGAGGCAACCTGAGGAGGCCAAAACTACACGGAGGCCGCCCCGCCAAGGAGGCGGG GCACGGGCCTGCGAGGAGGCCTCCAAGTGCCGCGTGGAAGCCGTGGACCGGGCCTGTTCC TGTGGCTACCTTGCCCACCGGGGGTCCTGGCCCCAGAAGGCCACCAGGCAGGCTGTTTTT GCTGAGGGGTCAGGTGACCCGGAATCCCGGTCGGCCGGGCTGGAAGAGGCTCCCCCGAGC CTGGGCTTCCTTCCACGGGCCGTGCGGAACTGAAGCAAAGAACTTCCCCGGGGTGGACCG GGCGCTTGGCTTGGCCCTACTGGCCCGGAGCAACAGAAGAGCGGGAGGAGGGCGGGTTCC CAAAAACTGGCTTTTGTAACAAAGATGGGCTCCTGGGAGTGACTGTTTACTTTTCCGGAA AATCCCCATTGGGAAACCCCCCCGTCTAATTTTCAACACTTAAAGGGGGTCTGAAAATGT CGGGCCCCAGCGGGTTCTGGTGGCTTACAACTGGGCTGGATAGGTTCCCCCCCCCCTTGA ACACCGCTGGGGCCGAAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003957 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003957.1, NP_003948.1 |
| RefSeq Size | 3516 bp |
| RefSeq ORF | 2007 bp |
| Locus ID | 9024 |
| UniProt ID | Q8IWQ3 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | Serine/threonine-protein kinase that plays a key role in polarization of neurons and axonogenesis, cell cycle progress and insulin secretion. Phosphorylates CDK16, CDC25C, MAPT/TAU, PAK1 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in postmitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Plays a role in the regulation of the mitotic cell cycle progress and the onset of mitosis. Plays a role in the regulation of insulin secretion in response to elevated glucose levels, probably via phosphorylation of CDK16 and PAK1. While BRSK2 phosphorylated at Thr-174 can inhibit insulin secretion (PubMed:22798068), BRSK2 phosphorylated at Thr-260 can promote insulin secretion (PubMed:22669945). Regulates reorganization of the actin cytoskeleton. May play a role in the apoptotic response triggered by endoplasmic reticulum (ER) stress.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223466 | BRSK2 (Myc-DDK-tagged)-Human BR serine/threonine kinase 2 (BRSK2) |
CNY 4896.00 |
|
| RC223466L1 | Lenti ORF clone of Human BR serine/threonine kinase 2 (BRSK2), Myc-DDK-tagged |
CNY 7296.00 |
|
| RC223466L2 | Lenti ORF clone of Human BR serine/threonine kinase 2 (BRSK2), mGFP tagged |
CNY 8930.00 |
|
| RC223466L3 | Lenti ORF clone of Human BR serine/threonine kinase 2 (BRSK2), Myc-DDK-tagged |
CNY 8930.00 |
|
| RC223466L4 | Lenti ORF clone of Human BR serine/threonine kinase 2 (BRSK2), mGFP tagged |
CNY 8930.00 |
|
| RG223466 | BRSK2 (tGFP-tagged) - Human BR serine/threonine kinase 2 (BRSK2) |
CNY 6496.00 |
