OSTC (NM_021227) Human Untagged Clone
CAT＃: SC100986
OSTC (untagged)-Human oligosaccharyltransferase complex subunit (OSTC)
CNY 1200.00
CNY 2950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DC2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC100986 sequence for NM_021227 edited (data generated by NextGen Sequencing)
ATGGAGACTTTGTACCGTGTCCCGTTCTTAGTGCTCGAATGTCCCAACCTGAAGCTGAAG AAGCCGCCCTGGTTGCACATGCCGTCGGCCATGACTGTGTATGCTCTGGTGGTGGTGTCT TACTTCCTCATCACCGGAGGAATAATTTATGATGTTATTGTTGAACCTCCAAGTGTCGGT TCTATGACTGATGAACATGGGCATCAGAGGCCAGTAGCTTTCTTGGCCTACAGAGTAAAT GGACAATATATTATGGAAGGACTTGCATCCAGCTTCCTATTTACAATGGGAGGTTTAGGT TTCATAATCCTGGACCGATCGAATGCACCAAATATCCCAAAACTCAATAGATTCCTTCTT CTGTTCATTGGATTCGTCTGTGTCCTATTGAGTTTTTTCATGGCTAGAGTATTCATGAGA ATGAAACTGCCGGGCTATCTGATGGGTTAG Clone variation with respect to NM_021227.2 >OriGene 5' read for NM_021227 unedited
GGCCGGTCAGCTTTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACCAGGGCGCT TGGGGCTTGAGGCCGAGACGGCCCTTGCTGCCACCAACATGGAGACTTTGTACCGTGTCC CGTTCTTAGTGCTCGAATGTCCCAACCTGAAGCTGAAGAAGCCGCCCTGGTTGCACATGC CGTCGGCCATGACTGTGTATGCTCTGGTGGTGGTGTCTTACTTCCTCATCACCGGAGGAA TAATTTATGATGTTATTGTTGAACCTCCAAGTGTCGGTTCTATGACTGATGAACATGGGC ATCAGAGGCCAGTAGCTTTCTTGGCCTACAGAGTAAATGGACAATATATTATGGAAGGAC TTGCATCCAGCTTCCTATTTACAATGGGAGGTTTAGGTTTCATAATCCTGGACCGATCGA ATGCACCAAATATCCCAAAACTCAATAGATTCCTTCTTCTGTTCATTGGATTCGTCTGTG TCCTATTGAGTTTTTTCATGGCTAGAGTATTCATGAGAATGAAACTGCCGGGCTATCTGA TGGGTTAGAGTGCCTTTGAGAAGAAATCAGTGGATACTGGATTTGCTCCTGTCAATGAAG TTNTAAAGGCTGTACCAATCCTCTAATATGAAATGTGGAAAAGAATGAAGAGCAGCAGTA AAAGAAATATCTAGTGAAAAAACAGGAAGCGTATTGAAGCTTGGACTAGAAATTCTTCTT GGTATTAAAGAGACAAGTTTATCACAGAATTTTTNTTTCCTGCTGGCCTATTGCTATACC AATGATGNNTGAGTGGCATTNTCTTTTTAGTTTTTCATTAAATATATTCCATATCTACCA CTATATATCAAATAAAGTGATTATTTTTTTACAACCTCCTTACATTTTTTGGAGAGAACT TTCTGATTTTCAGAATTAACATAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_021227 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021227.2, NP_067050.1 |
| RefSeq Size | 1103 bp |
| RefSeq ORF | 450 bp |
| Locus ID | 58505 |
| UniProt ID | Q9NRP0 |
| Protein Families | Transmembrane |
| Gene Summary | Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. May be involved in N-glycosylation of APP (amyloid-beta precursor protein). Can modulate gamma-secretase cleavage of APP by enhancing endoprotelysis of PSEN1.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208881 | OSTC (Myc-DDK-tagged)-Human oligosaccharyltransferase complex subunit (OSTC) |
CNY 1200.00 |
|
| RC208881L1 | Lenti ORF clone of Human oligosaccharyltransferase complex subunit (OSTC), Myc-DDK-tagged |
CNY 3600.00 |
|
| RC208881L2 | Lenti ORF clone of Human oligosaccharyltransferase complex subunit (OSTC), mGFP tagged |
CNY 5890.00 |
|
| RC208881L3 | Lenti ORF clone of Human oligosaccharyltransferase complex subunit (OSTC), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC208881L4 | Lenti ORF clone of Human oligosaccharyltransferase complex subunit (OSTC), mGFP tagged |
CNY 5890.00 |
|
| RG208881 | OSTC (tGFP-tagged) - Human oligosaccharyltransferase complex subunit (OSTC) |
CNY 2800.00 |
