IWS1 (NM_017969) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC100830 sequence for NM_017969 edited (data generated by NextGen Sequencing) ATGGACTCGGAATATTACAGCGGCGACCAGTCAGATGATGGTGGTGCTACCCCAGTACAG GATGAACGGGATTCAGGGTCAGACGGTGAGGATGATGTAAATGAGCAACACTCCGGATCA GACACTGGAAGTGTAGAACGTCATTCAGAGAATGAAACTAGTGATCGAGAAGATGGCCTC CCCAAAGGACATCATGTGACAGACTCTGAGAACGATGAGCCCTTAAATCTTAATGCTAGT GACTCTGAAAGTGAGGAGCTTCACAGGCAAAAGGACAGCGACTCTGAATCTGAGGAACGT GCAGAGCCTCCTGCAAGCGATTCTGAAAATGAGGATGTCAATCAGCATGGGAGCGACTCT GAGAGTGAAGAGACCAGGAAATTACCTGGTAGTGACTCTGAAAATGAGGAACTTCTTAAT GGGCATGCAAGTGACTCAGAAAACGAAGATGTTGGGAAGCATCCCGCCAGTGATTCTGAG ATTGAGGAGCTCCAGAAGAGTCCTGCTAGTGACTCTGAAACAGAAGATGCTCTAAAACCT CAAATCAGTGACTCTGAGAGTGAGGAACCCCCAAGGCACCAAGCCAGTGACTCCGAAAAT GAGGAGCCTCCCAAACCTCGAATGAGTGATTCTGAAAGTGAGGAGCTTCCTAAACCTCAG GTCAGTGATTCAGAAAGTGAGGAACCCCCAAGGCACCAGGCCAGTGACTCTGAAAATGAG GAGCTTCCCAAACCTCGTATCAGTGACTCAGAAAGTGAGGACCCTCCGAGGCACCAGGCC AGTGACTCAGAAAATGAAGAGCTTCCCAAACCCCGAATCAGTGATTCGGAAAGTGAGGAT CCCCCAAGGAACCAGGCCAGTGATTCGGAAAATGAGGAGCTACCCAAACCCCGAGTCAGT GACTCTGAGAGTGAGGTGCCTCAGAAGGGGCCTGCCAGTGACTCAGAAACTGAGGATGCG TCCAGACACAAACAGAAGCCAGAGTCAGATGATGACAGCGACAGGGAGAATAAGGGAGAG GATACAGAAATGCAGAATGACTCCTTCCATTCAGACAGCCATATGGACAGAAAAAAGTTT CACAGTTCTGATAGTGAGGAGGAAGAACACAAAAAGCAAAAAATGGACAGTGATGAAGAT GAAAAAGAGGGTGAGGAGGAGAAAGTAGCGAAGAGAAAAGCTGCTGTGCTTTCTGATAGT GAAGATGAAGAGAAAGCATCAGCAAAGAAGAGTCGTGTTGTCTCTGATGCAGATGACTCT GACAGTGATGCTGTATCAGACAAGTCAGGCAAAAGAGAGAAGACCATAGCATCTGACAGT GAGGAAGAAGCTGGGAAAGAATTGTCTGATAAGAAAAATGAAGAGAAGGATCTGTTTGGG AGTGACAGTGAGTCAGGCAATGAAGAAGAAAATCTTATTGCAGACATATTTGGAGAATCT GGTGATGAAGAGGAAGAAGAATTTACAGGTTTTAACCAAGAAGATCTGGAAGAAGAAAAA GGTGAAACACAGGTAAAAGAAGCAGAAGATTCAGATTCTGATGATAACATAAAGAGAGGA AAACATATGGACTTTCTGTCAGATTTTGAGATGATGTTGCAGCGAAAAAAGAGCATGAGT GGCAAGCGCAGACGGAACCGCGATGGTGGCACCTTTATTAGTGATGCAGACGACGTCGTG AGTGCCATGATCGTCAAGATGAATGAAGCTGCTGAGGAAGACAGACAGTTGAACAATCAA AAAAAGCCAGCACTGAAAAAATTAACTTTACTGCCTGCTGTAGTTATGCACCTTAAGAAG CAGGACCTTAAAGAAACATTCATTGACAGTGGTGTGATGTCTGCCATCAAAGAATGGCTC TCACCTCTACCAGATAGGAGTTTGCCTGCACTCAAGATCCGGGAGGAGCTGCTGAAGATC CTGCAAGAGCTGCCTAGTGTGAGCCAGGAGACCCTGAAGCATAGTGGGATTGGACGAGCA GTGATGTATCTCTATAAACACCCCAAGGAGTCAAGGTCTAACAAGGACATGGCAGGGAAA TTAATCAATGAGTGGTCTAGGCCTATATTTGGTCTTACCTCAAACTACAAAGGAATGACA AGAGAAGAAAGGGAGCAGAGAGATCTAGAACAGATGCCTCAACGACGAAGAATGAACAGC ACTGGTGGTCAGACACCCAGAAGAGACCTGGAAAAGGTGCTGACAGGAGAGGAGAAGGCT CTTAGACCTGGAGATCCTGGATTCTGTGCCCGTGCAAGGGTCCCAATGCCTTCAAACAAG GACTATGTTGTCAGGCCCAAATGGAATGTGGAAATGGAGTCATCCAGGTTTCAGGCGACC TCCAAGAAGGGTATCAGTCGACTGGATAAACAGATGAGAAAGTTCACAGATATAAGGAAA AAAAGCAGATCTGCACACGCAGTGAAAATCAGCATTGAGGGCAACAAAATGCCATTGTGA Clone variation with respect to NM_017969.2 917 g=>t 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_017969 unedited GGTGCAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGCTCGCATG CCTGCCCGCCCGCCAGCCTGCCGGGTACGGCCTTTTCCGCCGGGGCTTCCAGGTCAAAGA ATTCGCCTTTGCCGCTACCGCTTTCTTACCCTCCGCACCCGTTAAGTTCTCCGGTCGGGC GGCAGTCTCTGAACACTTAGCCGCGCCATCCGGGGTCACACCGCCTGGAAGGAGGTGACG GGGGCGGCGCGGGGCGCGGACACTCCCCGCTGAGAGTCCGCCTGCCATGGACTCGGAATA TTACAGCGGCGACCAGTCAGATGATGGTGGTGCTACCCCAGTACAGGATGAACGGGATTC AGGGTCAGACGGTGAGGATGATGTAAATGAGCAACACTCCGGATCAGACACTGGAAGTGT AGAACGTCATTCAGAGAATGAAACTAGTGATCGAGAAGATGGCCTCCCCAAAGGACATCA TGTGACAGACTCTGAGAACGATGAGCCCTTAAATCTTAATGCTAGTGACTCTGAAAGTGA GGAGCTTCACAGGCAAAAGGACAGCGACTCTGAATCTGAGGAACGTGCAGAGCCTNCTGC AAGCGATTCTGAAAATGAGGATGTCAATCAGCATGGGAGCGACTCTGAGAGTGAAGAGAC CAGGAAATTACCTGGTAGTGACTCTGAAAATGANGAACTTCTTAATGGGCATGCAAGTGA CTCAGAAACGAAGATGTTGGGAAGCATCCNGNCAGTGATTCTGAGATTGAGGAGCTCCAG AGAGTCCTGCTAGTGACTCTGAAACAGAAGATGCTCTTAAACCTCAAATCAGTGACTCTG AGAGTGAGGAACCCCCCAGGCACCAAGCCAGTGACTTCGGAAATGAGGAGCCTCCCAACC TCCAATGAGTGATTCTGAAAGTGAGAGCTTCCTAAACTCAG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_017969 unedited NNGGGTTCCACTATGNNACCGCGGCCGCATNCTAGNGATCGATTTTTTTTTTTTTTTTTT TAACCANAATGAAATTTTATTGTGTAAAGTTTATAGAAGTATGACTAGTATTCCTTTGTA CAAAGTACACAACGGTTTTAAATATAACTGAGAGAAATGTGAGTCCTATGACAACATCTG ATACACGCTGAACCATTTACAGACACACTAAAAATGTTTTAAAATATCTTCTTTCTCCAA AGAGTCCATTGCGCATTTCTTAGAGTAGAGATGGGGACACATTCCAGGCAAGGTCACAAT GGCATTTTGTTGCCCTCAATGCTGATTTTCACTGCGTGTGCAGATCTGCTTTTTTTCCTT ATATCTGTGAACTTTCTCATCTGTTTATCCAGTCGACTGATACCCTTCTTGGAGGTCGCC TGAAACCTGGATGACTCCATTTCCACATTCCATTTGGGCCTGACAACATAGTCCTTGTTT GAAGGCATTGGGACCCTTGCACGGGCACAGAATCCAGGATCTCCAGGTCTAAGAGCCTTC TCCTCTCCTGTCAGCACCTTTTCCAGGTCTCTTCTGGGTGTCTGACCACCAGTGCTGTTC ATTCTTCGTCGTTGAGGCATCTGTTCTAGATCTCTCTGCTCCCTTTCTTCTCTTGTCATT CCTTTGTAGTTTGAGGTAAGACCAAATATAGGCCTAGACCACTCATTGATTAATTTCCCT GCCATGTCCTTGTTAGACCTTGACTCCTTGGGGTGTTTATAGAGATACATCACTGCTCGT CCAATCCCACTATGCTTCAGGGNTCTCTGGCTCACACTANGCAGCTCTTGCAGGATCTTC AGCAGCTCCTCCCGGATACTGAGTGCAGGCAAACTCCTATCTGGTAGAAGTGN
Restriction Sites	NotI-NotI
ACCN	NM_017969
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_017969.1, NP_060439.1
RefSeq Size	2950 bp
RefSeq ORF	2460 bp
Locus ID	55677
UniProt ID	Q96ST2
Gene Summary	Transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. Acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription.[UniProtKB/Swiss-Prot Function]

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