Rtel1 (NM_001191857) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1306721
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217571 representing NM_001191857 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAGGGTAGTCCTGAATGGTGTGACAGTGGATTTCCCTTTCCAGCCCTACCCATGCCAGCAGGAAT ATATGACCAAGGTGCTAGAATGTCTCCAGAAGAAAGTGAATGGCATCCTCGAGAGCCCCACTGGCACTGG GAAGACGCTATGCCTCCTCTGCACTACATTGGCCTGGCGGGAACATCTCCGAGATGCCGTTTCTTCCCTA AAGATTGCTGAGAGAGTTCAAGGGGAACTCTTTGCCAGTCGGACCTTGTCATCCTGGAGGAGTGCTGCTG ATGCCAATGGAGACTCAATAGATTGCTACACAGATATCCCAAAGATCATCTATGCTTCTAGAACTCACTC ACAGCTAACTCAGGTCATCGGTGAGCTTCGGAATACTTCCTACCGGCCCAAGGTGTGTGTGCTGGGCTCC CGGGAGCAGCTGTGTATTCATCCCGAAGTGAAGAAGCAAGAGAGTAACCACATGCAGATCAGTTTGTGCC GTAAGAAGGTGGCAAGTCGCTCCTGTCATTTCTACAACAATGTGGAAGAGAAAAGCCTGGAGCAAGAGCT GGCTACCCCCATCTTGGATATTGAGGACCTTGTGAAGAACGGAAGCAAACACAAAGTGTGCCCATACTAC CTCTCTCGAAACCTGAAGCAGCAAGCTGATATCATCTTTATGCCATACAATTACTTGTTGGATGCTAAGA GTCGTAAGGCACACAACATTGACCTGAAGGGAACGGTTGTAATCTTTGATGAAGCTCACAATGTGGAAAA GATATGTGAGGAGTCAGCCTCCTTTGACTTGACTCCCCGAGATGTGGCTTCAGGACTGGAGGTTATCAAC CAGGTTTTGGAGGAGCAAGCCCGGGTGGCTCAGCATGGTGAACTCCAACAGGAGTTCATTATAGACACGT CCAGCTCAGGTCTCAACATGGAGCTGGAAGACATCGCAAAGTTGAAGATGATCCTGCTGCACCTGGAGGA GGCTATTGATGCTGTTCAGCTGCCTGGGGATGACAGAGGAGTCACCAAACCTGGAAGCTACATCTTCGAG CTGTTTGCTGAAGCACAAATAACATTTCAAACCAAAGGCTGCATTTTGGAGTCGCTGGACCAGATAATCC AGCACCTGACAGGACGCACTGGTGTGTTCACCAACACGGCTGGGTTGCAGAAGCTCATGGACATTATCCA GATTGTGTTCAGCGTGGACCCTCTGGAAGGCAGCCCTGGTTCTCAGGTGGGGCTGGGTAGCTCACATTTC TATAAGGTACACATACACCCTGAAACCAGCCACCGGAGAGCAGCTCAGCGGTCAGATGCCTGGAGTACCA CTGCATCCAGGAAACAAGGGAAGGTGCTGAGCTATTGGTGCTTCAGTCCCAGCCACAGCATGCGTGAACT GGTCCAGCAAGGAGTCCGTACCCTTATCCTCACCAGTGGTACACTGGCTCCGCTGTCTTCCTTTGCTCTG GAGATGCAAATTCCATTCCCAGTCTGTCTGGAGAATCCACACATCATTGACAAGAACCAGCTCTGGGTAG GGGTTATCCCCAGAGGACCTGATGGTGTTCAGCTAAGCTCTGCCTATGACAAAAGGTTTTCTGAAGAGTG CTTATCTTCCCTGGGGAAGGCTCTGGGCAACATTGCTCGTGTGGTGCCCCATGGGCTTCTGGTCTTCTTC CCTTCCTACCCTGTCATGGAGAAAAGCCTGGAGTTCTGGCAGGCGCAAGGCATGTCCAAAAAGGTAGAGG CACTGAAGCCTCTATTCGTGGAACCCAGGAACAAAGGCAGCTTCTCAGAGGTCATTGATGCCTACTACCA GCAAGTTGCCTCCCCTGGGTCTAATGGGGCCACCTTCTTAGCAGTGTGTCGAGGAAAGGCCAGTGAAGGG CTGGACTTCTCAGACATGAATGGTCGTGGTGTGATTGTCACGGGCCTCCCATATCCCCCACGCATGGATC CCCGTGTTATCCTCAAGATGCAGTTCTTGGATGAGATGAAAGGCCGGAGTAGGGTTGGAGGCCAGTGCCT CTCTGGACAGGAATGGTACCAGCAACAAGCATCCAGGGCTGTGAACCAGGCTATTGGGAGGGTTATCCGA CACCGCCATGACTATGGGGCCATCTTCTTATGTGACCACAGGTTTGCCTATGCTGATGCCAGGGCCCATC TGCCCTCCTGGGTGCGCCCCTACCTCAAGGTGTATGACAACTTTGGCCGTGTCATCCGAGATGTGGCCCA GTTCTTCCGTGTTGCTCAGAAAGCTATGCCTTTGCCAGTTCCTCAGGCTGTGACCTCAAGTGTGAGTGAG GGAGAAGCTGCTGTCAAGGAGGCCACATTGTCCAGCCACTCCCTCTCCACCAGGAAAGCCATGAGCTTGG ATGTGCATGTGCCCAGCCTGAGGCGGAGGCCCGTAGGATTACCGACTGCTGGAGACTCCGAGAGCAGCGT ATGTGTGGAGTATGAGCAGCAGACATTTTCTGCCCAGAAGAGACCCATGGGGCTGCTAGCTGCCTTAGAG TACAACGAGCAGAAGGCTGGGGCATCTGAGGAGCAGGCACTCAGCTCCTCCACCCCATCTCTCCGGTGTG AAAAGAGGCTGTCCGTTGAGCAAAGAGGAGGAAAGAAGAAAGTCAGGCTGGTCAACCATCCGGAGGAACC AGTGGCTGGCACACAGGCAGGCAGAGCCAAGATGTTCATGGTGGCCGTGAAGCAAGCACTGAGCCAAGCT AACTTTGACACCTTTACCCAGGCCCTGCAGCACTATAAGAGTTCTGATGACTTTGAAGCCCTAGTGGCCT CTCTCACCTGCCTCTTTGCTGAAGACCCCAAGAAACACACCCTACTTAAAGGTTTCTACCAGTTTGTACG ACCCCACCACAAGCAGCAGTTTGAGGACATCTGCTTCCAGCTAACAGGCCAACGATGTAGCTACCAGCCA GGAAACAGCCTTCCCTTTGGAGAGCAAGCACAGTCAACTGCCAGTAAGCAAGGAAGGAGAGAGCTGGAAT CTAAACTGACCTTGTCTGAAGGTGCAGATAGGCAGCTGGATCCTGGAGAGCACCTGAATCAGGGGTGGCC TCACCTGTCTACCCATCTAACCTCCAAAGGAGACACCAGCAATTGTCCAAAAGTGGGATGTGTGGGAGAA AAACCTGGCCAGCCTGCTGTGAATGACTACCTATCTGACGTCCACAAAGCTCTGGGATCTGCAAGCTGCA ACCAGCTTACGGCAGCTCTGAGGGCATACAAACAGGATGATGACCTGGACAAAGTGCTGGCTGTGGTGGC AGCATTGACCACTGCAAAACCTGAACACTTATCTTTGCTACAGAGATTTGGCATGTTTATACGTCGGCAT CATAAGCCTCGGTTTGTACAGACCTGTGCAGACCTAATGGGCCTACCTACCATAGGCAAGGGCTTGGAGC TCCCATGTCCCAGGGATGAGAGTACAACTGTGCCTTCTGAGCTTACTCATGAGGACATGAAACCAGGGCC CTCGACGTCCAAGAAACCTGAGAAGACCCAGAGTAAGATCTCATCCTTCCTTAGACAGAGGCCAGACCAG AGTGCGAGGTCTGATGATACCATAATGCAGCTTCCTCCCAGACTACCCCCTGAACACACGACGTCTCAGT GGAATTTTGTATGCCCAGCCTGTGCAACTGAGGATACAGTGCTTTTCCAGTGCCCTTCCTGTGACTTCTG CCGCTGCCGGGCCTGCTGGCAACGTCAACTCCAGGCCTCTAGACTGTGTCCAGCTTGTGGTGCTGTTAAC AGGAAGCAGAGCATTGCACAGGTCATCTGGCCGAAGCCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217571 representing NM_001191857 Red=Cloning site Green=Tags(s) MPRVVLNGVTVDFPFQPYPCQQEYMTKVLECLQKKVNGILESPTGTGKTLCLLCTTLAWREHLRDAVSSL KIAERVQGELFASRTLSSWRSAADANGDSIDCYTDIPKIIYASRTHSQLTQVIGELRNTSYRPKVCVLGS REQLCIHPEVKKQESNHMQISLCRKKVASRSCHFYNNVEEKSLEQELATPILDIEDLVKNGSKHKVCPYY LSRNLKQQADIIFMPYNYLLDAKSRKAHNIDLKGTVVIFDEAHNVEKICEESASFDLTPRDVASGLEVIN QVLEEQARVAQHGELQQEFIIDTSSSGLNMELEDIAKLKMILLHLEEAIDAVQLPGDDRGVTKPGSYIFE LFAEAQITFQTKGCILESLDQIIQHLTGRTGVFTNTAGLQKLMDIIQIVFSVDPLEGSPGSQVGLGSSHF YKVHIHPETSHRRAAQRSDAWSTTASRKQGKVLSYWCFSPSHSMRELVQQGVRTLILTSGTLAPLSSFAL EMQIPFPVCLENPHIIDKNQLWVGVIPRGPDGVQLSSAYDKRFSEECLSSLGKALGNIARVVPHGLLVFF PSYPVMEKSLEFWQAQGMSKKVEALKPLFVEPRNKGSFSEVIDAYYQQVASPGSNGATFLAVCRGKASEG LDFSDMNGRGVIVTGLPYPPRMDPRVILKMQFLDEMKGRSRVGGQCLSGQEWYQQQASRAVNQAIGRVIR HRHDYGAIFLCDHRFAYADARAHLPSWVRPYLKVYDNFGRVIRDVAQFFRVAQKAMPLPVPQAVTSSVSE GEAAVKEATLSSHSLSTRKAMSLDVHVPSLRRRPVGLPTAGDSESSVCVEYEQQTFSAQKRPMGLLAALE YNEQKAGASEEQALSSSTPSLRCEKRLSVEQRGGKKKVRLVNHPEEPVAGTQAGRAKMFMVAVKQALSQA NFDTFTQALQHYKSSDDFEALVASLTCLFAEDPKKHTLLKGFYQFVRPHHKQQFEDICFQLTGQRCSYQP GNSLPFGEQAQSTASKQGRRELESKLTLSEGADRQLDPGEHLNQGWPHLSTHLTSKGDTSNCPKVGCVGE KPGQPAVNDYLSDVHKALGSASCNQLTAALRAYKQDDDLDKVLAVVAALTTAKPEHLSLLQRFGMFIRRH HKPRFVQTCADLMGLPTIGKGLELPCPRDESTTVPSELTHEDMKPGPSTSKKPEKTQSKISSFLRQRPDQ SARSDDTIMQLPPRLPPEHTTSQWNFVCPACATEDTVLFQCPSCDFCRCRACWQRQLQASRLCPACGAVN RKQSIAQVIWPKPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191857
ORF Size	3822 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191857.1, NP_001178786.1
RefSeq Size	4237 bp
RefSeq ORF	3825 bp
Locus ID	362288
UniProt ID	Q5RJZ1
MW	141.8 kDa
Gene Summary	ATP-dependent DNA helicase implicated in telomere-length regulation, DNA repair and the maintenance of genomic stability. Acts as an anti-recombinase to counteract toxic recombination and limit crossover during meiosis. Regulates meiotic recombination and crossover homeostasis by physically dissociating strand invasion events and thereby promotes noncrossover repair by meiotic synthesis dependent strand annealing (SDSA) as well as disassembly of D loop recombination intermediates. Also disassembles T loops and prevents telomere fragility by counteracting telomeric G4-DNA structures, which together ensure the dynamics and stability of the telomere.[UniProtKB/Swiss-Prot Function]

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