Mgat4a (NM_001160155) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216865 representing NM_001160155 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCTCCGAAATGGAACTGTGGCCACTGCGCTGGTGTTTGTCACGTCCTTCCTTACCCTGTCCTGGT ATACCACATGGCAAAATGGGAAAGAAAAATTAATTGCTTACCAACGAGAATTCCTCGCTTTAAAAGAACG TCTTCGAGTGGCCGAACATAGGATATCTCAGCGCTCCTCTGAGCTAAACACCATTGTCCAACAGTTCCGC CGAGCTGGAGCGGAGACTAATGGAAGTAAGGCTGGTCTGAGTACGTTCTCAGATAATACCATAAAGCTTC TAAAAGAGTTGACAAGCAAAAAATCACTTCAAGTGCCAAGTATTTATTATCATTTGCCTCATCTATTGCA AAATGAAAGAAGCCTTCAGCCCGCCGTACAGATTGGCAGTGGAAGAACGGGAGTTTCAATAGTCATGGGG ATTCCTACTGTGAAGAGAGAAGTTAAATCTTACCTCATAGAGACCCTTCACTCCCTTATTGATAATCTGT ATCCTGAAGAGAAGCTGGACTGTGTTATAGTTGTCTTCATAGGAGAGACAGATCTTGATTATGTTCACAG CGTTGTTGCCAACCTGGAGAAAGAATTTTCTAGAGAAATTAGTTCCGGCTTGCTAGAAGTAATCTCTCCT CCTGAAAGCTATTACCCCGACTTAACAAACCTGAAGGAGACGTTCGGAGACTCCAAGGAAAGAGTAAGAT GGAGAACGAAGCAAAACCTGGACTATTGTTTTCTGATGATGTATGCTCAGGAGAAGGGCATTTACTACAT TCAGCTTGAAGATGATATTATTGTCAAGCAAAACTATTTTAATACCATAAAAAATTTTGCACTTCAACTT TCTTCTGAAGAATGGATGATTCTAGAGTTTTCCCAGCTGGGCTTCATTGGGAAGATGTTCCAGGCGCCGG ACCTAGCTCTGGTTGTGGAGTTCATTCTCATGTTCTATAAGGAGAAGCCCATTGACTGGCTCTTGGACCA CATTCTCTGGGTGAAGGTCTGCAACCCCGAAAAAGATGCCAAACACTGCGACAGACAGAAGGCAAACCTA CGAATCCGCTTCCGACCCTCCCTCTTCCAGCACGTGGGTCTACACTCATCTCTGTCGGGGAAAATTCAGA AACTTACGGATAAAGATTACATGAAACCATTGCTTCTCAAGATCCACGTGAACCCGCCTGCAGAGGTCTC CACTTCCCTGAAGGTGTACCAAGGGCACACACTGGAGAAGACCTACATGGGGGAAGACTTCTTTTGGGCC ATCACCCCCACAGCTGGAGACTACATCTTGTTTAAATTTGATAAACCGGTCAATGTGGAGAGTTATTTGT TCCACAGCGGCAATCAAGAGCACCCAGGGGACATCCTGCTGAACACGACCGTGGAGGTTCTGCCTCTTAA GAGCGACAGTTTGGAGATCAGCAAAGAAACCAAAGACAAACGATTAGAAGATGGCTATTTCAGAATAGGG AAATTTGAGTACGGAGTTGCAGAGGGAATTGTGGATCCTGGACTGAACCCTATCTCAGCCTTTCGACTGT CGGTTATTCAGAACTCTGCTGTCTGGGCCATTCTCAATGAGATTCATATTAAGAAAGTCACCAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216865 representing NM_001160155 Red=Cloning site Green=Tags(s) MRLRNGTVATALVFVTSFLTLSWYTTWQNGKEKLIAYQREFLALKERLRVAEHRISQRSSELNTIVQQFR RAGAETNGSKAGLSTFSDNTIKLLKELTSKKSLQVPSIYYHLPHLLQNERSLQPAVQIGSGRTGVSIVMG IPTVKREVKSYLIETLHSLIDNLYPEEKLDCVIVVFIGETDLDYVHSVVANLEKEFSREISSGLLEVISP PESYYPDLTNLKETFGDSKERVRWRTKQNLDYCFLMMYAQEKGIYYIQLEDDIIVKQNYFNTIKNFALQL SSEEWMILEFSQLGFIGKMFQAPDLALVVEFILMFYKEKPIDWLLDHILWVKVCNPEKDAKHCDRQKANL RIRFRPSLFQHVGLHSSLSGKIQKLTDKDYMKPLLLKIHVNPPAEVSTSLKVYQGHTLEKTYMGEDFFWA ITPTAGDYILFKFDKPVNVESYLFHSGNQEHPGDILLNTTVEVLPLKSDSLEISKETKDKRLEDGYFRIG KFEYGVAEGIVDPGLNPISAFRLSVIQNSAVWAILNEIHIKKVTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001160155
ORF Size	1605 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001160155.1, NP_001153627.1
RefSeq Size	7208 bp
RefSeq ORF	1608 bp
Locus ID	367252
UniProt ID	Q5M854
MW	61.5 kDa
Gene Summary	Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells (By similarity).[UniProtKB/Swiss-Prot Function]

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