Ipcef1 (NM_001170799) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216584 representing NM_001170799 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCGGAGAAGGATATCCTGTAAAGATCTGGGCCACGCTGACTGCCAGGGCTGGCTGTACAAGAAAA AGGAAAAGGGAACTTTCCTAAGCAACAAATGGAAAAAGTTCTGGGTGGTGCTGAAGGGGTCATCACTGTA CTGGTATGGCAATCAATTGGCAGAGAAAGCAGATGGATTTGTCAACCTGCCAGATTTCACAGTGGAAAGA GCCTCTGAATGCAAGAAAAAAAATGCATTTAAGATCAATCACCCACAGATCAAAACCTTCTACTTTGCAG CAGAGAATTTGCAGGAAATGAATATGTGGCTAAACAAACTTGGATTTGCTGTGACCCACAAGGAATCCAC TACCAAGGATGAAGAATGTTACAGCGAGAGCGAGCAGGAAGATCCTGAAACAGCCGTGGAGGCACCGCCC CCTCCATCCGCGTCTGCAACATCCTCTCCTGTGGCTGCACGGCGGGCATCTTCTTCTTCACCCAAACGGA GAGAAACATCATGTTCTTTCTCTTCTCTGGAAAATACAGTAAAGGCACCCAGCCGGTTTTCTTCCTCGGG ATCTAAAGAAAGACAGTCGTGGCTTGACATAGTTAACAGCTCGCCTGCTACCGAAGATGTGGGACATCCC CTGTCATTTGCTGTACAGGTCCACACACTTGCCTCCTCAGAGGCAAGCAGTTGCCGGGTCTCAGAAAACA GCTCCACAACACCAGAAAGTGGTTGTTTGAACTCTTTGTCTAGTGATGACACTTCTTCACTGAACAACAG TCAAGACCATCTAACAGTCCCAGACAGAGCTTCTGGATCAAGAATGACAGACAGAGACGAAATAAAATCG TCCGAAGACGATGAGATGGAGAAACTGTACAAGTCATTGGAGCAGGCTAGCCTGTCTCCTCTTGGGGACC GGCGACCTTCAACCAAGAAGGAGCTGAGAAAGTCTTTTGTGAAGCGATGCAAAAACCCATCAATAAACGA GAAGCTGCACAAGATCAGAACCTTGAACAGCACATTAAAGTGCAAAGAACATGATTTGGCTATGATCAAC CAGCTGCTGGATGACCCAAAGCTGACAGCCAGGAAGTACAGAGAGTGGAAAGTCATGAACACCCTGCTGA TCCAGGACATCTATCAGCAGCAGGTACCTCAGGACCCTGAAGTCACCCCTCAGGAAATCATGAACCCCAC CTCTTCTGACTGTGTGGAAAATTCTCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216584 representing NM_001170799 Red=Cloning site Green=Tags(s) MSRRRISCKDLGHADCQGWLYKKKEKGTFLSNKWKKFWVVLKGSSLYWYGNQLAEKADGFVNLPDFTVER ASECKKKNAFKINHPQIKTFYFAAENLQEMNMWLNKLGFAVTHKESTTKDEECYSESEQEDPETAVEAPP PPSASATSSPVAARRASSSSPKRRETSCSFSSLENTVKAPSRFSSSGSKERQSWLDIVNSSPATEDVGHP LSFAVQVHTLASSEASSCRVSENSSTTPESGCLNSLSSDDTSSLNNSQDHLTVPDRASGSRMTDRDEIKS SEDDEMEKLYKSLEQASLSPLGDRRPSTKKELRKSFVKRCKNPSINEKLHKIRTLNSTLKCKEHDLAMIN QLLDDPKLTARKYREWKVMNTLLIQDIYQQQVPQDPEVTPQEIMNPTSSDCVENSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001170799
ORF Size	1218 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001170799.1, NP_001164270.1
RefSeq Size	1517 bp
RefSeq ORF	1221 bp
Locus ID	361474
UniProt ID	Q80VL0
MW	45.7 kDa
Gene Summary	binds guanine nucleotide exchange factor cytohesin 2 and increases cytohesin 2 mediated formation of ADP-ribosylation factor GTP-ase [RGD, Feb 2006]

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