Scn5a (NM_013125) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Nav1.5; SCAL
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214460 representing NM_013125 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAACCTCCTGTTACCTCGGGGCACCAGCAGCTTCCGTAGGTTCACCCGGGAGTCACTGGCGGCCA TCGAGAAGCGAATGGCTGAAAAGCAAGCCCGAGGAGGTTCGGCCACCTCACAGGAGAGCCGTGAGGGCCT GCAGGAGGAGGAGGCTCCCCGGCCCCAGCTGGACCTACAGGCCTCCAAAAAGCTGCCAGATCTCTATGGC AACCCACCCCGAGAGCTCATCGGGGAGCCCCTGGAAGACCTGGACCCTTTCTATAGTACCCAGAAGACCT TCATCGTGCTGAATAAGGGCAAAACCATCTTCCGGTTCAGTGCCACCAATGCCTTGTATGTCCTCAGCCC CTTCCACCCCGTGCGCCGAGCGGCCGTGAAGATCCTGGTACACTCGCTCTTTAGCATGCTCATCATGTGC ACCATCCTGACCAACTGCGTGTTCATGGCCCAGCACGACCCTCCGCCTTGGACCAAATATGTTGAGTACA CCTTCACTGCCATCTACACCTTTGAGTCTCTGGTCAAGATTCTAGCTCGAGGCTTCTGCCTGCATGCATT CACCTTCCTTCGGGACCCGTGGAACTGGCTAGACTTCAGTGTGATCGTCATGGCATACACAACTGAATTT GTGGACCTGGGCAATGTCTCAGCCTTACGCACCTTCCGAGTCCTCCGGGCCCTGAAAACTATATCGGTCA TTTCAGGCCTGAAGACCATCGTGGGAGCCCTAATCCAGTCTGTGAAGAAACTGGCCGATGTGATGGTCCT CACTGTCTTCTGCCTCAGTGTCTTTGCCCTCATTGGCCTGCAGCTCTTCATGGGCAACCTGAGGCACAAG TGTGTGCGTAACTTCACCGAGCTCAATGGCACCAATGGTTCCGTGGAGGCCGACGGCCTAGTCTGGAACT CCCTGGACGTCTACCTCAATGACCCAGCCAATTACCTGCTCAAGAATGGCACCACGGATGTGTTACTATG TGGGAACAGCTCTGATGCCGGGACATGCCCTGAGGGCTATCGGTGCCTGAAGGCAGGTGAGAACCCAGAC CACGGTTACACCAGCTTCGACTCCTTCGCCTGGGCCTTCCTTGCACTCTTCCGCCTGATGACACAGGACT GCTGGGAACGCCTATACCAGCAGACCCTGAGGTCCGCAGGAAAGATCTACATGATCTTCTTCATGCTCGT CATCTTTCTGGGCTCCTTCTACCTGGTGAACTTGATCCTGGCTGTGGTGGCCATGGCCTACGAGGAGCAA AACCAAGCCACCATCGCCGAGACGGAAGAGAAGGAGAAGCGCTTCCAGGAGGCCATGGAGATGCTCAAGA AGGAACACGAGGCTCTCACCATCAGGGGTGTGGATACCGTGTCCCGTAGCTCTCTGGAGATGTCTCCTTT GGCCCCAGTAACCAACCATGAGAGAAAGAGCAAAAGGAGGAAACGACTATCTTCGGGGACAGAGGATGGT GGGGATGACAGGCTCCCCAAGTCGGACTCAGAAGATGGTCCCAGAGCATTGAATCAGCTCAGCCTCACCC ATGGGCTCAGCCGGACATCCATGAGGCCCCGCTCGAGCCGAGGGAGCATTTTCACGTTCCGAAGACGGGA CCAAGGCTCTGAGGCGGACTTCGCAGATGACGAGAACAGCACTGCGGGGGAGAGCGAGAGCCACCGCACA TCGCTGCTGGTACCCTGGCCCCTGCGCCATCCCAGCGCCCAAGGACAGCCCGGCCCTGGAGCCTCAGCTC CCGGTTACGTTCTCAATGGCAAAAGGAACAGCACCGTGGACTGCAATGGGGTGGTTTCCTTGCTGGGGGC AGGTGACGCAGAGGCCACCTCCCCAGGGAGCTACCTTCTCCGCCCTATGGTGCTGGACCGACCCCCAGAC ACGACCACTCCGTCAGAGGAGCCCGGTGGGCCCCAGATGCTGACACCTCAGGCTCCGTGTGCAGATGGTT TTGAGGAGCCCGGAGCACGGCAACGGGCACTCAGCGCTGTCAGTGTCCTCACCAGCGCCCTGGAAGAGTT GGAGGAGTCCCATCGGAAGTGTCCACCATGCTGGAACCGCTTTGCCCAGCACTACCTCATCTGGGAGTGC TGTCCACTCTGGATGTCCATCAAGCAGAAGGTGAAGTTTGTGGTCATGGACCCATTTGCCGACCTCACTA TCACCATGTGCATCGTGCTCAATACGCTCTTCATGGCTCTGGAGCATTACAACATGACGGCAGAGTTTGA GGAGATGCTGCAGGTCGGAAACCTGGTCTTCACGGGAATCTTCACAGCGGAGATGACCTTCAAGATCATC GCCCTTGACCCCTACTACTACTTCCAGCAGGGCTGGAATATCTTCGACAGCATCATCGTCATCCTCAGTC TCATGGAGCTGGGGCTGTCCCGCATGGGCAACTTGTCTGTGCTACGTTCCTTCCGTCTGCTGCGGGTCTT CAAGCTGGCCAAGTCCTGGCCCACCCTGAACACGCTCATCAAGATCATCGGAAACTCCGTGGGCGCCCTG GGGAACCTGACCCTGGTGCTGGCCATCATCGTCTTCATCTTCGCCGTGGTGGGCATGCAGCTCTTCGGCA AGAACTACTCAGAACTGAGGCACCGCATCAGCGACTCCGGCCTGCTGCCCCGCTGGCACATGATGGACTT TTTCCACGCCTTCCTCATCATCTTCCGCATCCTCTGTGGGGAGTGGATCGAGACCATGTGGGACTGCATG GAGGTGTCTGGGCAGTCGCTGTGCTTGCTGGTCTTCCTGCTCGTCATGGTCATTGGCAACCTTGTGGTCC TGAATCTCTTCTTGGCCTTGCTGCTCAGCTCCTTCAGCGCAGACAACCTCACAGCCCCTGACGAGGATGG GGAGATGAACAACCTCCAGCTGGCCCTGGCTCGCATCCAGAGGGGCCTGCGCTTTGTCAAGCGGACCACC TGGGACTTCTGCTGCGGGATCCTGCGGCGGCGACCTAAGAAGCCCGCGGCTCTTGCCACCCACAGCCAGC TGCCCAGCTGTATCACCGCCCCCAGGTCCCCACCACCCCCAGAGGTGGAGAAGGTGCCCCCAGCCCGCAA GGAAACACGATTCGAGGAGGACAAGCGACCCGGCCAGGGCACCCCTGGGGATTCGGAGCCTGTGTGTGTG CCCATCGCCGTGGCTGAGTCAGACACTGAAGACCAGGAAGAGGATGAAGAGAACAGCCTTGGCACAGAGG AAGAGTCCAGCAAACAGGAATCCCAAGTTGTGTCTGGTGGCCACGAGCCCTACCAGGAGCCCAGGGCCTG GAGCCAGGTGTCAGAGACCACGTCCTCTGAAGCTGGGGCCAGTACATCTCAGGCAGACTGGCAGCAAGAG CAGAAAACGGAGCCCCAGGCCCCGGGGTGCGGTGAGACCCCTGAGGACAGTTACTCCGAGGGCAGCACAG CTGACATGACCAACACCGCCGACCTCCTGGAGCAAATCCCAGACCTTGGTGAGGACGTCAAGGACCCAGA GGACTGCTTTACTGAAGGCTGCGTCCGACGCTGTCCCTGCTGCATGGTAGACACAACCCAGTCCCCAGGG AAGGTCTGGTGGCGATTGCGCAAGACCTGCTACCGCATCGTGGAGCACAGCTGGTTCGAGACTTTCATCA TCTTCATGATCCTGCTCAGCAGTGGAGCGCTGGCCTTCGAGGACATCTACCTGGAGGAGCGGAAGACCAT CAAGGTTCTGCTGGAGTACGCGGACAAGATGTTCACCTACGTCTTTGTGTTGGAGATGCTGCTCAAGTGG GTGGCCTACGGCTTCAAGAAGTACTTCACCAACGCCTGGTGCTGGCTGGACTTCCTGATTGTGGACGTCT CGCTGGTCAGCCTCGTGGCAAACACCTTAGGCTTCGCCGAAATGGGTCCCATCAAGTCACTGAGGACACT GCGTGCACTTCGACCCCTGAGGGCCTTGTCGAGATTTGAGGGCATGCGGGTGGTGGTCAATGCGCTGGTG GGCGCCATCCCCTCCATCATGAACGTCCTCCTCGTCTGCCTCATCTTCTGGCTCATCTTCAGCATCATGG GCGTGAACCTCTTCGCCGGGAAGTTCGGTAGGTGCATCAACCAGACAGAAGGGGACCTGCCTCTGAACTA CACCATCGTGAACAACAAGAGTGAGTGCGAGTCCTTCAACGTGACCGGAGAGTTGTACTGGACCAAGGTG AAGGTCAACTTTGACAACGTGGGAGCCGGGTACCTGGCCCTCCTGCAGGTGGCGACATTTAAAGGCTGGA TGGACATCATGTATGCGGCTGTGGACTCCAGAGGGTATGAGGAGCAGCCGCAGTGGGAAGACAACCTCTA CATGTACATCTACTTTGTCGTCTTCATCATCTTCGGCTCCTTCTTCACCCTCAACCTCTTCATCGGTGTC ATCATTGACAACTTCAACCAGCAGAAGAAAAAGTTAGGGGGCCAGGATATCTTCATGACGGAGGAGCAGA AGAAGTACTACAATGCCATGAAGAAGCTGGGCTCCAAGAAACCCCAGAAGCCCATCCCACGGCCCTTGAA CAAGTACCAGGGTTTCATATTCGACATTGTGACCAAGCAGGCCTTCGATGTCACCATCATGTTCCTCATC TGTTTGAACATGGTGACCATGATGGTGGAGACAGATGACCAGAGCCCTGAGAAGGTCAACATCTTGGCCA AGATCAACCTGCTCTTCGTGGCCATCTTCACAGGCGAGTGTATTGTCAAGATGGCTGCCCTGCGCCACTA TTACTTCACCAACAGCTGGAACATCTTCGACTTTGTGGTGGTCATCCTCTCCATTGTTGGCACTGTCCTC TCCGACATCATCCAGAAGTACTTCTTCTCCCCGACACTCTTCCGGGTCATCCGTCTGGCCAGGATCGGCC GCATCCTCAGGCTGATCCGCGGAGCCAAGGGGATTCGCACGCTGCTCTTCGCCCTCATGATGTCCCTGCC CGCCCTCTTCAACATCGGCCTCCTCCTCTTCCTCGTCATGTTCATCTACTCCATCTTCGGCATGGCCAAC TTCGCTTACGTCAAGTGGGAGGCCGGCATCGATGACATGTTCAACTTCCAGACCTTCGCCAACAGCATGC TGTGCCTGTTCCAGATCACCACATCAGCCGGCTGGGACGGCCTCCTCAGCCCCATCCTCAACACGGGGCC TCCCTACTGCGACCCCAACCTGCCCAACAGCAACGGCTCCCGGGGGAACTGTGGGAGCCCGGCGGTGGGC ATCCTCTTCTTCACCACCTACATCATCATCTCCTTCCTCATCGTGGTCAACATGTACATCGCCATCATCC TCGAGAACTTCAGCGTGGCCACCGAGGAGAGCACAGAGCCCCTGAGCGAGGACGACTTCGACATGTTCTA TGAGATCTGGGAGAAGTTCGACCCGGAGGCCACCCAGTTCATTGAGTATCTGGCCCTGTCCGACTTTGCA GATGCCTTGTCTGAGCCGCTCCGCATCGCCAAACCCAACCAGATAAGCCTCATCAACATGGATCTGCCCA TGGTGAGCGGAGACCGTATCCACTGTATGGACATACTGTTCGCTTTCACCAAGAGGGTGCTCGGCGAGTC TGGGGAGATGGATGCCCTGAAGATCCAGATGGAGGAGAAGTTCATGGCGGCCAACCCTTCCAAGATCTCC TACGAGCCCATCACCACCACCCTGAGGAGAAAGCACGAGGAGGTGTCGGCCACGGTCATCCAGCGTGCCT TCCGGAGGCACCTGCTGCAGCGCTCGGTGAAGCATGCCTCCTTTCTCTTCCGCCAGCAAGCGGGCGGCAG TGGCCTCTCCGACGAGGATGCCCCTGAGCGGGAGGGCCTCATCGCCTACATGATGAATGGGAACTTCTCT CGGCGCAGTGCTCCGCTCTCCAGCTCCTCCATCTCCTCCACGTCCTTCCCCCCGTCCTACGACAGCGTCA CGAGAGCCACCAGTGATAACCTCCCGGTGCGTGCGTCTGACTATAGCCGCAGCGAAGATCTTGCAGACTT CCCTCCATCTCCAGATAGGGACCGAGAGTCTATCGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214460 representing NM_013125 Red=Cloning site Green=Tags(s) MANLLLPRGTSSFRRFTRESLAAIEKRMAEKQARGGSATSQESREGLQEEEAPRPQLDLQASKKLPDLYG NPPRELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPVRRAAVKILVHSLFSMLIMC TILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIVMAYTTEF VDLGNVSALRTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHK CVRNFTELNGTNGSVEADGLVWNSLDVYLNDPANYLLKNGTTDVLLCGNSSDAGTCPEGYRCLKAGENPD HGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQ NQATIAETEEKEKRFQEAMEMLKKEHEALTIRGVDTVSRSSLEMSPLAPVTNHERKSKRRKRLSSGTEDG GDDRLPKSDSEDGPRALNQLSLTHGLSRTSMRPRSSRGSIFTFRRRDQGSEADFADDENSTAGESESHRT SLLVPWPLRHPSAQGQPGPGASAPGYVLNGKRNSTVDCNGVVSLLGAGDAEATSPGSYLLRPMVLDRPPD TTTPSEEPGGPQMLTPQAPCADGFEEPGARQRALSAVSVLTSALEELEESHRKCPPCWNRFAQHYLIWEC CPLWMSIKQKVKFVVMDPFADLTITMCIVLNTLFMALEHYNMTAEFEEMLQVGNLVFTGIFTAEMTFKII ALDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGAL GNLTLVLAIIVFIFAVVGMQLFGKNYSELRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCM EVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFVKRTT WDFCCGILRRRPKKPAALATHSQLPSCITAPRSPPPPEVEKVPPARKETRFEEDKRPGQGTPGDSEPVCV PIAVAESDTEDQEEDEENSLGTEEESSKQESQVVSGGHEPYQEPRAWSQVSETTSSEAGASTSQADWQQE QKTEPQAPGCGETPEDSYSEGSTADMTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCMVDTTQSPG KVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKW VAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALV GAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSECESFNVTGELYWTKV KVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEDNLYMYIYFVVFIIFGSFFTLNLFIGV IIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLI CLNMVTMMVETDDQSPEKVNILAKINLLFVAIFTGECIVKMAALRHYYFTNSWNIFDFVVVILSIVGTVL SDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMAN FAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVG ILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDFA DALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKIS YEPITTTLRRKHEEVSATVIQRAFRRHLLQRSVKHASFLFRQQAGGSGLSDEDAPEREGLIAYMMNGNFS RRSAPLSSSSISSTSFPPSYDSVTRATSDNLPVRASDYSRSEDLADFPPSPDRDRESIV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_013125
ORF Size	6057 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_013125.2, NP_037257.1
RefSeq Size	8468 bp
RefSeq ORF	6060 bp
Locus ID	25665
UniProt ID	P15389
MW	227.4 kDa
Gene Summary	acts as a tetrodotoxin-resistant voltage gated sodium channel; may play a role in cardiac function [RGD, Feb 2006]

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