Gcc2 (NM_001107633) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Lims1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213995 representing NM_001107633 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATTCTGCTCAAGATGCTGTGACTGCAGCACCCTCCGGGACCCCGAAATCCAAGCTAGAAACAT TGCCCAGAGAAGATCTCATCAAGTTTGCCAAGAAGCAGATGATGCTCCTACAGAAAGCCAAAGCAAGGTG TACAGAATTAGACAAAGAAGTTGAAGAGCTCAAATCAAAACCTGTTGACGGAGGAACTGATGATATGATT AAGGTGCTAACTGAACGCTTGGATGCTCTTCTTCTGGAAAAAGCAGAGACTGAACAACAGTGTCTGTGTC TCAAAAAAGAAAATATTAAAATGAAGCAAGAGGTTGAGGATTCTGTAACTAAACTGGAAGAGACACAAAA AGAGTTTGAACAATCACATAGAAACTACGTAAGAGAAATGGAAAGTGTTAAAAATGAACTGATAGCAGTA CACTCAGAGCACAGCAAAGAAAAGGCTGCCTTACAAAGAGACCTGGAGGGAGCAGTCCATAGGCAAGCAG AGCTGTTAGAACAGCTCAAGTCTCAGAGCGACTCTGAAGATAATGTTAAAAAACTACAGGAAGAGATTCA GAACATTACAGCAGCATTTGAGGAACAAACGTCATGTCTACAAAAGCAATTAGAAGCTACCAGTGATGAA AAACAGCAAGAGATTATTCATCTCCAGAAAGTCATTGAGGACAACGCTCAGCACTACCAAAAAGATATTA ATACTTTCCAAGAAGAGATTGTGCAGCTGAGAGCTACACACAAAGAAGAAGTTAATGAGCTGATGTCTCA AATGGAAACATTAGCTAAAGAACATGAGGCAGCAGTAAATAAGCTAAAAGAGAACAGAGTGACACTGTGT GAGACAAGTGAGACCATTCCAGAGAATTACCAATGTGAATCAGAAAGCTTAAATGAAGACACCTCAGATG CAAGCCAGGAGAATCAAAAATGCTCTGTGGCCTTACAGGAAGATCCTTTTGCAGAACATACAGTGTATGA TAAAGTCAGACAATTGGAAGATTCATTAAAAGAACTGGAATCTCAACATAGTATCTTAAAAGATGAAGTG ACTTATATGAATAATCTCAAGTTAAAACTTGAAATGGATGCCCAGCATATAAAGGATGAGTTTTTCCATG AAAGAGAAGACTTGGAGTTTAAAATTAATGAACTGTTGCTGGCCAAAGAAGAACAGAGCTACGTAGTAGA AAAATTAAAATATGAGCGAGAAGATTTAAATAGGCAGCTTTGCTGTACTGTGGAACAACATAACAAAGAA ATACAGCGTCTTCAGGAGCACCATCAGAAAGAAATCTCTGAACTAAGTGAGACATTTATGTCAGGTTCAG AAAAAGAAAAGTTAGCATTAATGTTTGAAATACAGGGACTTAAGGAACAGTGTGAAAATCTACAACATGA AAAGCAAGAGGTAGTTCTAAATTATGAGAGTTTACGAGAGATGATGGAAATTTTACAAACGGAACTTGGA GAATCTGCTGGAAAAATAAGTCAAGAGTTTGAAACAATGAAGCAACAGCAAGCATCTGATGTTCATGAGC TTCAGCAGAAGCTAAGAACTGCTTTTAACGAAAAAGACGCTCTTCTTGAAACTATAAATCGCCTACAAGG AGAAAATGAAAAATTATTATCACAAGAATTGGTATCAGAACTCGAAAGTACCATGAAGAACCTTAAGGCA GATAATAGCATGTACTTAGCCAGCCTTGGTCAAAAAGATACCCTGTTACAAGAATTAGAAGCAAAGATAA GTTCTCTTGCTGAAGAAAAAGATGATTTTATAAGTAAAATCAAAACTTCCCGTGAAGAGATAGATGATCT CCATCAGAAATGGGAAAGGGAACAGAAACTGTCTGTAGAACTCAGGGAAGCAGCAGAGCAAGCCGCCCAG CACAACAGTGAACTGAGACAAAGAGTAAGTGAATTAACAGGAAAACTAGATGAAATATTAAGAGAAAAGA GTCAAAATGACCAAAACATTATGGTTCAAATGAAAACCATGACAGAAGACCAAGAAGCATTGTCATCTAA AATCAAGTCTCTTTATGAGGAAAACAATAGACTGCATTCTGAAAAGGTCCAGTTGAGTAGAGATTTAGAA GCTCTTCAGTCTCAACAAGATTTTGCCTATAAAGAGCATGTTGCTGAATTCGAAAAGAAACTCCAGTTAA TGGTTGAAGAACGAGATGATTTAAATAAACTGCTTGAAAATGAGCAACTTCAGAAGTCATTTGTCAAAAC TCAGTTGTACGAGTTTCTTAAGCAAATGAGGCCAAGCATTTTAGAAGATAATGAAGAGGAAGATGTTGTC ACAGTTCTAAAAGCTGTGGGTGAATCCTTAGTGACAGTAAAGGAAGAAAAGCATAACTTAGTCTTTGAAT ATGATGCGAGAGTATTAGAATTAGAAAGGAGGATTAAGTGCCTTCAAGAGGAGAGTGTAGTTCAGTGTGA AGAACTAAGGGCTTTAGTAAGAGACTCTGAACAAGAGAAAATTCTCCTAAGAAAAGAATTAGATGAAGTC ACATCAACAAAAGAGGCCCTGCAGTGTGATATTCTAGAAATGAAGAATACTAATGAAAAAACAAGCCTTG AAAATCAGACTCTTTCAACTCGGGTTGAAGAATTATCGCGGTCATTACACAGCAAAAATGAAGTCCATAA TGAAAAGGATCTTGTCATAGAACATGAAAACCTGAGGCTATCGCTTGAGCAGAGGGAGTCTGAGCTACAA GATGTGAGAGCAGAATTGATGCTGCTGAAGGATTCCTTAGAGAAATCGCCTTCTGTAAAAAATGATCAAC TTTCATTGGTAAAAGAGCTGGAAGAAAAAATAGAAAGTCTGGAAAAAGAATCCAAAGACAAGGATGAGAA AATCAGTAAGATAAAACTAGTCGCTGTGAGAGCAAAGAAAGAACTAGATTCTAACAGGAAAGAGGCCCAG ACACTACGGGACGAACTCGAGTCCGTACAGTCAGAAAAGGACCGCTTATCTGCTTCTATGAAGGAGTTTA TCCAAGGAGCAGAAAGCTATAAGAATCTTCTGTTAGAATATGACAAGCAGTCAGAACAGCTGGATGTGGA AAAAGAACGTGCTAATAATTTTGAACATCACATAGAAGACCTTACCAAACAATTAAGAGATTCGACTTGC CAGTATGAAAAACTAACTTCCGACAATGAGGATCTCCTGGCTCGAATTGAGACCCTGCAGGCTAATGCCA GGTTATTAGAAGCACAGATCTTAGAGGTCCAGAGAGCCAAAGGAGTAGTAGAAAAAGAACTGGAAGCTGA AAAACTTCAGAAAGAGCAGAAAATTAAGGAACATGTCAGTACTACAAATGAGCTTGAAGAACTTCAACTC CAATTTCAAAAGGAAAAGAAGCAGCTTCAGAAAACGATGCAAGAATTAGAACTGGTTAAAAAGGATGCCC AACAGACCACGTTGATGAATATGGAAATAGCAGATTACGAACGTTTGATGAAAGAATTAAATCAAAAGTT AACAAATAAAAACAGTAAGATAGAAGATTTGGAGCAAGAAATGAAAATTCAGAAACAGAAACAAGAAACC CTACAAGAAGAAATGACTTCCCTGCAGTCTTCAGTACAGCACTACGAAGAAAAGAACGCCCAAATCAAGC AGCTGCTTGTGAAAACCAAAAAGGAACTGGCAGATGCAAAGCAAGCAGAAACTGATCACTTACTACTCCA GGCGTCTTTAAAGGGCGAGCTGGAAGCAAGCCAGCAGCAAGTAGAAGTCTATAAAATTCAGCTAGCTGAG ATGACATCTGAGAAACACAAAATCCATGAGCACCTGAAGACTTCTGCGGAACAGCACCAGCGCACACTAA GTGCATACCAGCAGAGGGTGGTGGCCCTGCAGGAAGAGAGCCGCACTGCCAAGGCAGAGCAAGCTGCTGT CACCTCTGAATTTGAGAACTACAAAGTCCGGGTTCATAACGTTCTAAAACAACAGAAAAATAAATCTGTG TCTCAGGCTGAAACTGAGGGAGCTAAACAAGAAAGGGAACACCTGGAAATGCTGATTGACCAACTGAAAA TCAAGTTACAGGACAGCCAAAACAGCTTACAAATCAGTGTGTCAGAGTTCCAGACACTGCAGTCTGAGCA TGACACTCTGCTAGAGAGGCACAACCGGATGCTGCAGGAAACTGTGACCAAAGAGGCGGAACTTCGGGAA AAGTTATGTTCAGCTCAATCTGAGAACACCATGTTGAAGTCTGAACACGCCCAGACAATGTGTCAGCTGA CATCCCAGAATGAGGCCCTCCGTAACAGCTTCCGAGACCAAGTTCGACACTTGCAGGATGAGCACAGAAA GACAGTGGAGACCTTGCAGCACCAGCTCTCCAAGGTGGAAACTCAGCTCTTCCAGCTCAAGAGTGAGCCA CCCACAAAAAGCCCTGCTTCTTCCCATCAACCTTCAAAAAGCCTCCGAGAAAGACGGACCACAGACCTCC CCCTTCTGGACATGCACACTGTGACCCGAGAGGAGGGGGAAGGGATGGAGACAACTGACAGTGAGTCTGT GTCTTCGGCCGGCACACACATTCAGTCCTTGGAGCAGCTCCTTAGCTCTCCTGACAGCAAGCTTGAACGT CTTACAGAGGCCTCCTTATGGCATACAGAATTTACCAAAGAAGAATTGGCTGAGAAGCTCAGTTCCACCA CGAAGAGTGCAGATCACTTAAATGGCCTGCTTCGGGAGACAGAGGCTACCAACGCCATCCTTATGGAGCA AATTAAGCTTCTCAAAAGTGAAATAAGAAGACTGGAAAGAAACCAAGAGCGGGAGAAGTCTGTGGCTAAC CTGGAGTACTTGAAGAATGTCCTACTGCGGTTCATTTTCCTGAAGCCGGGTAGTGAAAGAGAGAGGCTTC TTCCTGTGATAGACACGATGCTGCAGCTCAGCCCTGAAGAAAAGGGAAAGCTTGCCACAGTTGCTCAAGG TGAGGAAGAAAGTGCCTCTCGTTCCTCTGGATGGGCATCCTACCTTCATAGCTGGTCTGGACTGCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213995 representing NM_001107633 Red=Cloning site Green=Tags(s) MEDSAQDAVTAAPSGTPKSKLETLPREDLIKFAKKQMMLLQKAKARCTELDKEVEELKSKPVDGGTDDMI KVLTERLDALLLEKAETEQQCLCLKKENIKMKQEVEDSVTKLEETQKEFEQSHRNYVREMESVKNELIAV HSEHSKEKAALQRDLEGAVHRQAELLEQLKSQSDSEDNVKKLQEEIQNITAAFEEQTSCLQKQLEATSDE KQQEIIHLQKVIEDNAQHYQKDINTFQEEIVQLRATHKEEVNELMSQMETLAKEHEAAVNKLKENRVTLC ETSETIPENYQCESESLNEDTSDASQENQKCSVALQEDPFAEHTVYDKVRQLEDSLKELESQHSILKDEV TYMNNLKLKLEMDAQHIKDEFFHEREDLEFKINELLLAKEEQSYVVEKLKYEREDLNRQLCCTVEQHNKE IQRLQEHHQKEISELSETFMSGSEKEKLALMFEIQGLKEQCENLQHEKQEVVLNYESLREMMEILQTELG ESAGKISQEFETMKQQQASDVHELQQKLRTAFNEKDALLETINRLQGENEKLLSQELVSELESTMKNLKA DNSMYLASLGQKDTLLQELEAKISSLAEEKDDFISKIKTSREEIDDLHQKWEREQKLSVELREAAEQAAQ HNSELRQRVSELTGKLDEILREKSQNDQNIMVQMKTMTEDQEALSSKIKSLYEENNRLHSEKVQLSRDLE ALQSQQDFAYKEHVAEFEKKLQLMVEERDDLNKLLENEQLQKSFVKTQLYEFLKQMRPSILEDNEEEDVV TVLKAVGESLVTVKEEKHNLVFEYDARVLELERRIKCLQEESVVQCEELRALVRDSEQEKILLRKELDEV TSTKEALQCDILEMKNTNEKTSLENQTLSTRVEELSRSLHSKNEVHNEKDLVIEHENLRLSLEQRESELQ DVRAELMLLKDSLEKSPSVKNDQLSLVKELEEKIESLEKESKDKDEKISKIKLVAVRAKKELDSNRKEAQ TLRDELESVQSEKDRLSASMKEFIQGAESYKNLLLEYDKQSEQLDVEKERANNFEHHIEDLTKQLRDSTC QYEKLTSDNEDLLARIETLQANARLLEAQILEVQRAKGVVEKELEAEKLQKEQKIKEHVSTTNELEELQL QFQKEKKQLQKTMQELELVKKDAQQTTLMNMEIADYERLMKELNQKLTNKNSKIEDLEQEMKIQKQKQET LQEEMTSLQSSVQHYEEKNAQIKQLLVKTKKELADAKQAETDHLLLQASLKGELEASQQQVEVYKIQLAE MTSEKHKIHEHLKTSAEQHQRTLSAYQQRVVALQEESRTAKAEQAAVTSEFENYKVRVHNVLKQQKNKSV SQAETEGAKQEREHLEMLIDQLKIKLQDSQNSLQISVSEFQTLQSEHDTLLERHNRMLQETVTKEAELRE KLCSAQSENTMLKSEHAQTMCQLTSQNEALRNSFRDQVRHLQDEHRKTVETLQHQLSKVETQLFQLKSEP PTKSPASSHQPSKSLRERRTTDLPLLDMHTVTREEGEGMETTDSESVSSAGTHIQSLEQLLSSPDSKLER LTEASLWHTEFTKEELAEKLSSTTKSADHLNGLLRETEATNAILMEQIKLLKSEIRRLERNQEREKSVAN LEYLKNVLLRFIFLKPGSERERLLPVIDTMLQLSPEEKGKLATVAQGEEESASRSSGWASYLHSWSGLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107633
ORF Size	5037 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107633.1, NP_001101103.1
RefSeq Size	6526 bp
RefSeq ORF	5040 bp
Locus ID	309798
UniProt ID	D3ZZL9
MW	195.1 kDa
Gene Summary	Golgin which probably tethers transport vesicles to the trans-Golgi network (TGN) and regulates vesicular transport between the endosomes and the Golgi. As a RAB9A effector it is involved in recycling of the mannose 6-phosphate receptor from the late endosomes to the TGN. May also play a role in transport between the recycling endosomes and the Golgi. Required for maintenance of the Golgi structure, it is involved in the biogenesis of noncentrosomal, Golgi-associated microtubules through recruitment of CLASP1 and CLASP2 (By similarity).[UniProtKB/Swiss-Prot Function]

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