United States
Germany
Japan
United Kingdom
China
Irak1 (NM_001127555) Rat Tagged ORF Clone
CAT＃: RR212987
- TrueORF®
Irak1 (Myc-DDK-tagged ORF) - Rat interleukin-1 receptor-associated kinase 1 (Irak1), (10 ug)
"NM_001127555" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 7,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RGD1563841 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR212987 representing NM_001127555
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGGGGCCCGGGCCCCGGGGAGCCTGTGGTTCCCGGCGCCCAGCATTTCTTGTACGAGGTGCCTC CCTGGGTTATGTGCCGTTTCTACAAAGTGATGGACGCCCTGGAGCCCGCCGACTGGTGCCAGTTCGCGGC CTTGATCGTGCGCGACCAGACAGAGCTGCGGCTGTGTGAGCGCTCCGAGCAGCGCACGGCCAGTGTCCTG TGGCCCTGGATCAACCGCAACGCGCGTGTGGCTGACCTCGTTCACATCCTCACGCACCTGCAGCTGCTGC GTGCGCGGGACATCATTACAGCCTGGCACCCTCCTGCCTCTGTTCTGCCCCCAAGCACCGGTGCCCCAAG GCCCAGCAGCATCTCTGCAGGCTCTGAGACTGAGGACTGGAGCCCCCGGAAATTGCAGTCCTCTGCCTCC ACCTTCCTCTCCCCAGCTTTTCCAGGCTCCCAGACCCATTCTGAGTCAGAGCTCCTCCAGGTTCCACTCT CTGATTCCCTCGGGCCACCACTACAATCTTCAGCCCCCTCCTCCATCAAGCCAAGCCCAGAGAGCCCAGT GTCTGGCCTCCAAAGAGCTCGTCCCTCCCCATTTTGCTGGCCCTTCTGTGAGATTTCCCAAGGCACTTGC AACTTCTCTGAAGAACTCAGGATTGGAGAGGGTGGTTTTGGGTGTGTGTACCGAGCAGTAATGAGAAATA CTACATATGCTGTGAAGAGACTGAAGGAGGAGGCTGACCTAGAGTGGACTGTGGTGAAACAGAGCTTCCT AACAGAGGTGGAACAGCTATCAAGGTTTCGTCACCCAAATATCGTGGACTTTGCTGGCTACTGTGCAGAG AGTGGCTTCTACTGCCTTGTTTATGGCTTCCTGCCCAATGGCTCCTTAGAGGATCAGCTTCACCTTCAGA CCCAAGCCTGCTCCCCACTTTCCTGGCCTCAACGACTGGACATTCTTCTGGGCACAGCCCGGGCTATTCA GTTTTTACATCAGGACAGCCCCAGCCTTATCCATGGAGACATCAAGAGTTCTAATGTGCTTCTGGATGAG AGACTGATGCCCAAGCTGGGAGACTTTGGTCTGGCTCGTTTCAGCCGCTTTGCAGGGGCCAACCCAAGCC AGAGCAGTACTGTGGCCCGGACTTGCACAGTTCGAGGTACCCTCGCCTACCTGCCTGAGGAGTACATCAA GACAGGCCGGCTGGCTGTGGACACCGATACCTTCAGCTTTGGGGTGGTAATACTGGAGACCCTTGCTGGT CAGAGGGCTGTGAGGACACAAGGTGCAAAAACCAAATATCTGAAAGACCTGATTGAAGATGAGGCTGAAG AGGTGGGAGTGACCTTAAAAAGCACCCAGCCTACTTTGTGGATGGGTGTAGCCACGGATGCTTGGACTGC TCCAATTGCTGCCCAGATCTATAAGAAGCACCTGGACTCCAGACCTGGGCCCTGCCCACCCCAGTTGGGC CTGGCCCTGGCTCAACTAGCTTGCTGCTGCATGCACCGTCGGGCCAAGAAGAGGCCACCCATGACCCAGG TATACAAGAGACTAGAAGGGCTTCAGGCAGTACCTCCCTGGAAGCTAGAGGTTGCCGGCCATGGCTCTCC TTCCCCACAGGAGAACTCCTACATGTCCACCACTGGCAGTGCCCAGAGTGGGGATGAACCATGGCAGCCT CTGGTAGTGACCACAAGAGCTCCAGCCCAGGCTGCCCAGCAACTCCAGAGAAGTCCCAACCAGCCAGTGG AGAGTGATGAGAGTGTTCCTGGCCTCTCTGCTACCCTGCATTCCTGGCACTTGACTCCAGATTCCCACCC AAGCCCTGTGTCCTTCAGAGAGGCTAGCTGTACACAAGGAGGCACTACCAGAGAATCAAGTTTGAGGAGT GGCCCAGGCTTCCAGCCTACGACCATGGAAGGCTCATCGATGGGCAGTTCATCCCTGCTGTCATCAGAGC CACCACAGATCATCATCAACCCAGCCCGACAGAAGATGGTACAAAAGCTGGCCCTGTATGAAGAGGGGGT CTTGGATAGCCTGCAACTGCTGTCATCAGGCTTTTTCCCAGGCTTGGATTTAGAACCTGAAAAGAGAAAG AGACCTGAAGAAAGTGATGAATTCCAGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR212987 representing NM_001127555
Red=Cloning site Green=Tags(s) MAGGPGPGEPVVPGAQHFLYEVPPWVMCRFYKVMDALEPADWCQFAALIVRDQTELRLCERSEQRTASVL WPWINRNARVADLVHILTHLQLLRARDIITAWHPPASVLPPSTGAPRPSSISAGSETEDWSPRKLQSSAS TFLSPAFPGSQTHSESELLQVPLSDSLGPPLQSSAPSSIKPSPESPVSGLQRARPSPFCWPFCEISQGTC NFSEELRIGEGGFGCVYRAVMRNTTYAVKRLKEEADLEWTVVKQSFLTEVEQLSRFRHPNIVDFAGYCAE SGFYCLVYGFLPNGSLEDQLHLQTQACSPLSWPQRLDILLGTARAIQFLHQDSPSLIHGDIKSSNVLLDE RLMPKLGDFGLARFSRFAGANPSQSSTVARTCTVRGTLAYLPEEYIKTGRLAVDTDTFSFGVVILETLAG QRAVRTQGAKTKYLKDLIEDEAEEVGVTLKSTQPTLWMGVATDAWTAPIAAQIYKKHLDSRPGPCPPQLG LALAQLACCCMHRRAKKRPPMTQVYKRLEGLQAVPPWKLEVAGHGSPSPQENSYMSTTGSAQSGDEPWQP LVVTTRAPAQAAQQLQRSPNQPVESDESVPGLSATLHSWHLTPDSHPSPVSFREASCTQGGTTRESSLRS GPGFQPTTMEGSSMGSSSLLSSEPPQIIINPARQKMVQKLALYEEGVLDSLQLLSSGFFPGLDLEPEKRK RPEESDEFQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001127555 |
| ORF Size | 2130 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001127555.1, NP_001121027.1 |
| RefSeq Size | 2749 bp |
| RefSeq ORF | 2133 bp |
| Locus ID | 363520 |
| MW | 77.9 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN212987 | Irak1 (untagged ORF) - Rat interleukin-1 receptor-associated kinase 1 (Irak1), (10 ug) |
CNY 9,120.00 |
|
| RR212987L3 | Lenti ORF clone of Irak1 (Myc-DDK-tagged ORF) - Rat interleukin-1 receptor-associated kinase 1 (Irak1), (10 ug) |
CNY 10,930.00 |
|
| RR212987L4 | Lenti ORF clone of Irak1 (mGFP-tagged ORF) - Rat interleukin-1 receptor-associated kinase 1 (Irak1), (10 ug) |
CNY 12,070.00 |
