Zeb2 (NM_001033701) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Zfhx1b
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR212086 representing NM_001033701 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCAGCCGATCATGGCGGATGGCCCCCGGTGCAAGAGGCGCAAACAAGCCAATCCCAGGAGGAAAA ACGTGGTGAACTATGACAACGTAGTGGACGCAGGTTCCGAAACAGACGAGGAGGACAAGCTGCACATTGC TGAGGATGACGGCATTGCAAACCCTCTGGACCAGGAGACGAGTCCAGCTAGCATGCCCAACCACGAGTCC TCCCCACACATGAGCCAAGGCCTGCTACCAAGAGAGGAAGAAGAAGACGAGATAAGGGAGAGCGTTGTGG AACACAGCTGGCACAGCGGTGAGATTCTGCAAGCCTCTGTAGACGGTCCAGAAGAAATGAAGGAGGATTA TGATGCTCTGGGGCCAGAAGCCACGATCCAGACCACAATTAACAATGGTACAGTTAAGAATGCAAATTGC ACATCAGACTTTGAGGAATACTTTGCCAAAAGAAAACTGGAGGAAAGAGATGGCCACGCAGTCAGCATTG AAGAGTACCTTCAGCGAAGCGACACGGCCATTATTTACCCAGAAGCCCCTGAGGAGCTGTCTCGCCTTGG CACGCCAGAGGCCAATGGGCAAGAAGAAAATGACCTGCCACCTGGAACTCCAGATGCTTTTGCCCAACTG CTGACCTGCCCCTACTGCGACCGCGGCTACAAGCGCTTGACATCGCTGAAGGAGCACATCAAGTACCGCC ACGAGAAGAATGAAGAGAACTTTTCCTGCCCTCTTTGTAGCTACACGTTCGCCTACCGCACCCAGCTCGA AAGGCATATGGTGACGCACAAGCCAGGGACAGATCAGCACCAAATGCTAACCCAAGGAGCAGGTAACCGC AAGTTCAAGTGCACAGAGTGTGGCAAGGCCTTCAAGTACAAGCACCACCTGAAAGAACACCTGAGAATTC ACAGTGGTGAAAAACCTTACGAATGCCCCAACTGCAAGAAACGCTTCTCTCATTCTGGTTCCTACAGTTC ACATATCAGCAGCAAGAAATGTATTGGTTTAATCTCAGTAAATGGCCGAATGAGAAACAATATCAAGACG GGTTCTTCCCCTAATTCTGTTTCTTCTTCTCCTACTAACTCAGCCATTACTCAACTAAGGAACAAGTTGG AAAATGGAAAACCACTTAGCATGTCTGAGCAGACGGGCTTACTTAAGATTAAAACAGAACCACTAGACTT CAATGACTATAAAGTTCTTATGGCAACACATGGGTTTAGTGGCACTAGTCCCTTTATGAACGGTGGGCTT GGAGCCACCAGCCCTTTAGGTGTGCACCCATCTGCTCAGAGTCCAATGCAGCACTTAGGTGTAGGGTTAG AAGCCCCTTTACTTGGATTTCCCACTATGAATAGTAATCTGAGTGAGGTACAAAAGGTTCTACAGATTGT GGATAATACGGTTTCTAGGCAAAAGATGGACTGCAAGACTGAAGACATTTCAAAGTTGAAAGGTTACCAC ATGAAGGATCCATGTTCTCAGCCAGAAGAACAAGGGGTTACTTCTCCTAGTATTCCAGCCGTCGGTCTTC CAGTAGTGAGTCATAATGGTGCCACTAAAAGTATCATTGACTATACCTTAGAGAAAGTCAACGAAGCCAA AGCTTGCCTCCAGAGCTTGACTACTGACTCAAGGAGACAGATCAGCAATATAAAGAAAGAGAAGCTACGT ACTTTGATAGATTTGGTCACCGATGATAAAATGATTGAGAACCACAGCATACCCACTCCGTTTTCATGCC AGTTCTGTAAAGAAAGCTTCCCAGGTCCTATCCCCCTGCATCAGCATGAACGCTACCTGTGTAAAATGAA TGAAGAGATCAAGGCGGTCCTGCAACCTCACGAAAACATAGTCCCCAACAAAGCTGGGGTGTTCGTTGAC AATAAAGCCCTCCTCTTGTCATCTGTACTTTCTGAGAAAGGACTGACAAGCCCGATCAACCCGTACAAGG ACCACATGTCTGTACTCAAAGCATACTATGCTATGAATATGGAACCCAACTCTGATGAACTGCTGAAAAT CTCCATTGCTGTGGGCCTTCCTCAGGAGTTTGTGAAGGAATGGTTTGAGCAGAGAAAAGTCTACCAGTAC TCCAGTTCCAGGTCACCATCACTGGAGAGGAACTCCAAGCCCTTAGCTCCCAACAGCAACCCCACCACAA AAGACTCTTTGTTACCCAGGTCTCCTGTAAAACCTATGGACTCAATCACGTCACCATCTATAGCAGAACT CCACAACAGTGTCACGAGTTGTGATCCTCCTCTCAGGCTAACAAAATCTTCCCACTTCACCAATACTAAA GCAGTTGATAAACTGGACCACTCAAGGAGTAACACTCCTTCTCCTTTAAATCTTTCTTCCACGTCTTCTA AAAACTCCCACAGTAGCTCGTACACTCCAAATAGCTTCTCTTCCGAGGAGCTGCAGGCTGAGCCGTTGGA CCTGTCATTACCAAAACAAATGAGAGAGCCCAAAAGTATTATAGCCACAAAGAACAAGACGAAAGCTACT AGCATAAGTTTAGACCACAACAGTGTTTCTTCATCCTCTGAAAACTCAGATGAACCTCTGAATTTGACTT TTATCAAGAAAGAGTTTTCAAATTCTAATAACCTGGACAATAAAAGCACTAACCCTGTGTTCGGCATGAA CCCATTTAGTGCCAAGCCTTTATACACAGCTCTTCCACCACAGAGCGCATTTCCCCCTGCCACTTTCATG CCACCAGTCCAGACCAGCATCCCCGGGCTACGACCATACCCAGGGCTGGATCAGATGAGCTTCCTACCAC ATATGGCCTATACCTACCCAACGGGAGCAGCTACCTTTGCTGATATGCAGCAAAGGAGGAAATACCAGCG GAAACAAGGATTTCAGGGAGAATTGCTTGATGGAGCACAAGACTACATGTCGGGCCTAGATGACTTGACA GACTCCGATTCCTGTCTGTCTCGAAAGAAGATAAAGAAGACAGAAAGTGGCATGTATGCATGTGACTTAT GTGACAAGACATTCCAGAAAAGCAGTTCCCTTCTGCGACATAAATACGAACACACAGGAAAGAGACCACA CCAGTGTCAGATTTGTAAGAAAGCGTTCAAACACAAACACCACCTTATCGAGCACTCGAGGCTGCACTCG GGCGAGAAGCCCTATCAGTGTGACAAATGTGGCAAGCGCTTTTCACACTCGGGCTCCTACTCGCAGCACA TGAATCACAGGTACTCCTACTGCAAGCGGGAGGCGGAGGAGCGGGAAGCGGCCGAGCGCGAGGCACGAGA GAAAGGGCACTTGGAACCCACCGAGCTGCTGATGAACCGGGCTTACTTGCAGAGCATCACCCCTCAGGGG TACTCTGACTCGGAGGAGAGGGAGAGCATGCCGAGGGATGGCGAGAGCGAGAAGGAGCACGAGAAGGAGG GCGAGGAGGGCTATGGGAAGCTGCGGAGAAGGGACGGCGACGAGGAGGAAGAGGAGGAAGAGGAAGAAAG TGAAAATAAAAGTATGGATACGGATCCCGAGACGATACGGGATGAGGAGGAGACTGGGGATCACTCAATG GATGACAGTTCAGAGGATGGGAAAATGGAAACCAAATCAGACCACGAGGAAGACAATATGGAAGACGGCA TGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR212086 representing NM_001033701 Red=Cloning site Green=Tags(s) MKQPIMADGPRCKRRKQANPRRKNVVNYDNVVDAGSETDEEDKLHIAEDDGIANPLDQETSPASMPNHES SPHMSQGLLPREEEEDEIRESVVEHSWHSGEILQASVDGPEEMKEDYDALGPEATIQTTINNGTVKNANC TSDFEEYFAKRKLEERDGHAVSIEEYLQRSDTAIIYPEAPEELSRLGTPEANGQEENDLPPGTPDAFAQL LTCPYCDRGYKRLTSLKEHIKYRHEKNEENFSCPLCSYTFAYRTQLERHMVTHKPGTDQHQMLTQGAGNR KFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGLISVNGRMRNNIKT GSSPNSVSSSPTNSAITQLRNKLENGKPLSMSEQTGLLKIKTEPLDFNDYKVLMATHGFSGTSPFMNGGL GATSPLGVHPSAQSPMQHLGVGLEAPLLGFPTMNSNLSEVQKVLQIVDNTVSRQKMDCKTEDISKLKGYH MKDPCSQPEEQGVTSPSIPAVGLPVVSHNGATKSIIDYTLEKVNEAKACLQSLTTDSRRQISNIKKEKLR TLIDLVTDDKMIENHSIPTPFSCQFCKESFPGPIPLHQHERYLCKMNEEIKAVLQPHENIVPNKAGVFVD NKALLLSSVLSEKGLTSPINPYKDHMSVLKAYYAMNMEPNSDELLKISIAVGLPQEFVKEWFEQRKVYQY SSSRSPSLERNSKPLAPNSNPTTKDSLLPRSPVKPMDSITSPSIAELHNSVTSCDPPLRLTKSSHFTNTK AVDKLDHSRSNTPSPLNLSSTSSKNSHSSSYTPNSFSSEELQAEPLDLSLPKQMREPKSIIATKNKTKAT SISLDHNSVSSSSENSDEPLNLTFIKKEFSNSNNLDNKSTNPVFGMNPFSAKPLYTALPPQSAFPPATFM PPVQTSIPGLRPYPGLDQMSFLPHMAYTYPTGAATFADMQQRRKYQRKQGFQGELLDGAQDYMSGLDDLT DSDSCLSRKKIKKTESGMYACDLCDKTFQKSSSLLRHKYEHTGKRPHQCQICKKAFKHKHHLIEHSRLHS GEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAEEREAAEREAREKGHLEPTELLMNRAYLQSITPQG YSDSEERESMPRDGESEKEHEKEGEEGYGKLRRRDGDEEEEEEEEESENKSMDTDPETIRDEEETGDHSM DDSSEDGKMETKSDHEEDNMEDGME TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001033701
ORF Size	3645 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001033701.1, NP_001028873.1
RefSeq Size	3648 bp
RefSeq ORF	3648 bp
Locus ID	311071
MW	136.5 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.