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Polk (NM_138516) Rat Tagged ORF Clone
CAT＃: RR211062
- TrueORF®
Polk (Myc-DDK-tagged ORF) - Rat polymerase (DNA directed) kappa (Polk), (10 ug)
"NM_138516" in other vectors (3)
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CNY 10,830.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Dinb1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR211062 representing NM_138516
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAACACAAAGGAAAAGAACAACTTCAAAGACGCCCTTCTGCTCCGCATGGGACTAAATGATAACA AGGCAGGCATGGAAGGGTTGGATAAGGAGAAAATTAACAAAATTATCATGGAAGCCACAAAGGGGTCCAG ATTTTATGGAAATGAACTCAAGAAGGAGAAGCAAGTCAATCAACGGATTGAAAACATGATGCAGCAAAAG GCGCAAATTACCAGCCAGCAACTAAGGAAGGCCCAGCTACAGGTTGACAGATTTGCGATGGGATTAGAAC GGAACCGGAATTTGAACAACACCATAGTTCACGTGGACATGGATGCTTTCTATGCGGCCGTAGAAATGAG GGACAACCCGGAACTGAAGGAGAAGCCCATCGCTGTAGGATCCATGAGCATGCTGGCTACTTCAAATTAC CACGCAAGGAGGTTTGGGGTTCGCGCAGCCATGCCAGGATTTATTGCCAAGAGACTCTGCCCACAACTTA TTATAGTGCCCCCAAACTTTGACAAGTATAGAGCTGTGAGTAAGGAGGTTAAGGAGATACTCGCTGAATA TGATCCCAATTTTATGGCCATGAGTCTGGACGAGGCCTACTTGAATATAACACAGCACTTACAGGAAAGG CAAGGTTGGCCTGAGGACAAAAGAAGATATTTCATCAAAATGGGAAACTGTAGAAAAATTGACACACCCC GGCAGGAAGCTGATGAGCTGACTGAGCGGGAGCGCTCCATCTCCCCACTGCTTTTTGAAGATAGCCCTCC TGATTTGCAACCCCAAGGAAGTCCTTTCCAACTGAACTCTGAAGAACAAAACAATCCTCAAATAGCCCAA AATCCAGTTGTTTTTGGAACATCAGCTGAGGAAGTGGTAAAGGAAATTCGCTTCAGAATTGAACAGAAAA CAACGCTAACAGCCAGCGCAGGCATCGCCCCTAATACAATGTTAGCAAAAGTGTGCAGTGATAAGAATAA ACCAAATGGACAGTACCAGATCGTCCCCAGCAGGAGCGCAGTGATGGACTTCATCAAGGACTTGCCTATT CGAAAGGTTTCTGGAATAGGAAAAGTTACGGAGAAAATGTTGCTGGCTCTCGGGATTGTTACTTGCACAG AACTCTACCAACAGAGAGCGTTGCTTTCTCTCCTTTTCTCGGAGTCATCCTGGCACTATTTTCTTCATGT TGCACTGGGCCTGGGTTCAACAGACCTGGCGAGGGATGGAGAAAGGAAAAGTATGAGCGTTGAAAGGACA TTTAGTGAGATCAATAAAACAGAGGAACAGTACAGCTTGTGCCAAGAACTGTGCACTGAGCTCGCCCACG ATCTCCAGAAGGAAGGACTTAAGGGCAGAACTGTCACCATCAAGCTGAAGAATGTGGATTTTGAAGTAAA AACCCGCGCATCTACAGTACCGTCCGCCATTTCTACTGCAGAGGACATATTTGCCGTTGCCAAGGAGCTG CTAAGGACAGAAGTCACTGCAGGCTCTCCACAGCCCCTGCGGCTAAGACTGATGGGTGTTCGGATGTCTA CTTTCTCCAACGACGATGACAGGAAGCACCAACAAAGGAGCATCATTGGCTTCTTACAAGCTGGAAACCA AGGTTTGTCACCCACTGGGTGTACTGCAGACAGAACTGACAAAACTGAGCTCGTAAAGCCCTTAGAAACG TCTCGTAAGAAGAGTTTCTTTGATAAAAAGCGATCAGAAAGGATCCCGAACTGTCAAGACCCACCCAGAT GTAAAATCGCGGGTGAGCAAGCTTTACAGGCCTCGGAACCATCCCAAGCGTTGAAGAAGATGAGCGAGAG TTTCAAAACCTCAGAGAATTCAAATGACCCTCAGACATTTATATGTCCAGTTTGCTTTAGGGAGCAAGAA GGCATCAGTCTGGAAGCCTTTAATGAACATGTAGATGCATGTCTCGATGGACCGTCCACCAGTGAGAACT CAGAGATGCCCAGTTACTCACATGCTTCGTCTGCGGACATTGGTCAGCAGGCACACCGTGCTGTTCCACT GTGGGAGAAACGGGGGCATGGAGATGGAGAGATCACCTCAGGAGATGGTGCAGATCTAACAGAGACTGAA GACAGATCGCCGAAAGCAGCAAGCATGGACGCTGTAGGGAATAATCACAGCGAAGGGGAGGGATATCCTG ATAGTCAGGGCAAGTCCTGCCCTCTGTCACCCGCAAACGAAACTGTCAGCACGTTAAGTAGGGAAGACTC TGCCCAGCCCTGTACAGATGAGGTAGTGACAGGACGAGCTCTAGTTTGTCCCGTTTGTAACCTAGAACAA GAGACGTCTGATCTCACCCTCTTCAACATCCACGTGGACATTTGCTTAAATAAAGGCATCATCCAAGAAC TGAGAAACAGCGAAGTTAATTCAGTTAAGCAACCCAAAGGGAGCACGAGAAGCACTGACAGACTTCAGAA GGCTTCAGGAAAGACAAAAAGGGCAGGCCGGAAGACAAAGGGCTCAACATCGAAGAGAACAAAACCCCGA GATCCCAGACACACCCTTGATGGATTTTTTAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR211062 representing NM_138516
Red=Cloning site Green=Tags(s) MDNTKEKNNFKDALLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQK AQITSQQLRKAQLQVDRFAMGLERNRNLNNTIVHVDMDAFYAAVEMRDNPELKEKPIAVGSMSMLATSNY HARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILAEYDPNFMAMSLDEAYLNITQHLQER QGWPEDKRRYFIKMGNCRKIDTPRQEADELTERERSISPLLFEDSPPDLQPQGSPFQLNSEEQNNPQIAQ NPVVFGTSAEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQIVPSRSAVMDFIKDLPI RKVSGIGKVTEKMLLALGIVTCTELYQQRALLSLLFSESSWHYFLHVALGLGSTDLARDGERKSMSVERT FSEINKTEEQYSLCQELCTELAHDLQKEGLKGRTVTIKLKNVDFEVKTRASTVPSAISTAEDIFAVAKEL LRTEVTAGSPQPLRLRLMGVRMSTFSNDDDRKHQQRSIIGFLQAGNQGLSPTGCTADRTDKTELVKPLET SRKKSFFDKKRSERIPNCQDPPRCKIAGEQALQASEPSQALKKMSESFKTSENSNDPQTFICPVCFREQE GISLEAFNEHVDACLDGPSTSENSEMPSYSHASSADIGQQAHRAVPLWEKRGHGDGEITSGDGADLTETE DRSPKAASMDAVGNNHSEGEGYPDSQGKSCPLSPANETVSTLSREDSAQPCTDEVVTGRALVCPVCNLEQ ETSDLTLFNIHVDICLNKGIIQELRNSEVNSVKQPKGSTRSTDRLQKASGKTKRAGRKTKGSTSKRTKPR DPRHTLDGFFK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138516 |
| ORF Size | 2553 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138516.1, NP_612525.1 |
| RefSeq Size | 3821 bp |
| RefSeq ORF | 2556 bp |
| Locus ID | 171525 |
| MW | 95.1 kDa |
| Gene Summary | may mediate the increase of A:T to C:G transversions [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN211062 | Polk (untagged ORF) - Rat polymerase (DNA directed) kappa (Polk), (10 ug) |
CNY 10,830.00 |
|
| RR211062L3 | Lenti ORF clone of Polk (Myc-DDK-tagged ORF) - Rat polymerase (DNA directed) kappa (Polk), (10 ug) |
CNY 12,730.00 |
|
| RR211062L4 | Lenti ORF clone of Polk (mGFP-tagged ORF) - Rat polymerase (DNA directed) kappa (Polk), (10 ug) |
CNY 13,870.00 |
