Pcdh17 (NM_001107279) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210629 representing NM_001107279 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACCTTTCCATCTGTTGCTGCTTTCTTTTGTGGGCCCCTGCCCTGACACTCAAAAACCTCAACTATT CAGTACCAGAGGAGCAAGGGGCCGGCACGGTGATAGGCAACATCGGCAAGGATGCCCGACTGCAGCCCGG GCTTCCGCCCGCTGAGCGCGGCAGCGGCAGCGGGAGGAGCAAGTCCGGCAGCTACCGGGTGCTGGAGAAC TCCGCACCACATCTGCTGGACGTGGATGCTGACAGCGGGCTCCTCTACACCAAACAGCGAATCGACCGTG AGTCCTTATGCCGCCACAATGCCAAGTGCCAGCTCTCCCTAGAGGTGTTCGCTAACGACAAGGAGATTTG CATGATCAAGGTAGAGATCCAGGACATCAACGACAACGCTCCCTCTTTTCCCTCAGATCAGATAGAAATG GACATCTCCGAGAACGCGGCACCCGGCACCCGCTTCCCCCTTACGAGCGCTCACGACCCAGACGCTGGCG AGAACGGGCTCCGCACCTATCTGCTCACCCGCGATGATCATGGCCTCTTTGCACTGGACGTCAAGTCCCG CGGCGACGGCACCAAGTTCCCAGAGCTAGTCATCCAAAAGGCGTTGGACCGCGAGCTGCAGAACCACCAC ACTCTGGTGCTGACAGCCCTGGATGGCGGGGAGCCTCCACGCTCCGCTACGGTGCAGATCAATGTGAAGG TGATCGATTCCAATGACAACAGTCCAGTCTTCGAGGCTCCATCGTACTTGGTGGAACTGCCCGAGAACGC TCCACTGGGTACGGTGGTCATTGATCTGAACGCCACCGATGCTGATGAAGGACCCAACGGAGAAGTCCTC TACTCCTTTAGTAGCTATGTGCCTGACCGCGTGCGGGAGCTCTTCTCCATCGATCCTAAAACTGGCCTGA TCCGCGTTAAGGGCAACCTGGACTATGAGGAGAACGGCATGTTAGAGATCGACGTGCAGGCCAGAGACCT AGGACCTAACCCAATTCCAGCCCATTGCAAGGTCACAGTCAAGCTCATCGACCGCAACGATAACGCGCCG TCCATTGGTTTCGTCTCCGTGCGCCAGGGGGCGCTGAGTGAGGCCGCCCCGCCTGGCACAGTCATCGCCC TAGTTCGGGTCACTGACCGAGACTCAGGCAAGAATGGGCAGCTTCAGTGTCGAGTTCTAGGTGGAGGAGG GACTGGGGGTGGAGGTGGCCTGGGAGGTCCTGGTTCCGTCCCCTTCAAGCTGGAAGAAAACTATGACAAC TTCTACACCGTGGTGACTGACCGTCCGCTGGACCGTGAGACACAAGACGAGTACAATGTGACAATAGTGG CCCGGGACGGCGGCTCCCCTCCACTCAACTCCACCAAGTCCTTCGCTGTCAAGATTCTGGATGAGAATGA CAACCCGCCTCGTTTCACCAAAGGACTGTACGTGCTCCAAGTACATGAGAACAATATTCCAGGAGAATAC CTCGGGTCAGTGCTCGCCCAGGATCCCGACCTAGGCCAAAATGGGACAGTGTCATACTCCATTCTCCCCT CACACATCGGTGACGTGTCCATCTACACCTATGTGTCCGTGAACCCCACTAACGGGGCCATATATGCCCT GCGCTCCTTTAACTATGAACAGACGAAGGCTTTTGAATTCAAGGTGCTGGCTAAGGACTCGGGGGCGCCC GCGCATTTGGAGAGCAACGCCACGGTGAGGGTGACAGTGTTAGACGTGAATGACAACGCTCCAGTGATTG TGCTTCCCACTCTACAGAACGACACCGCTGAACTGCAGGTCCCGCGCAATGCTGGCCTGGGGTACCTGGT AAGCACAGTTCGAGCCCTGGACAGCGACTTTGGAGAGAGCGGGCGCCTCACCTATGAGATTGTGGATGGC AATGATGACCACCTGTTTGAGATCGATCCGTCCAGCGGCGAGATCCGCACGCTGCACCCCTTCTGGGAGG ATGTGACCCCGGTGGTGGAGCTTGTGGTGAAGGTGACCGATCATGGCAAGCCCACCCTGTCCGCCGTGGC CAAACTTATCATTCGCTCCGTGAGTGGTTCCCTGCCTGAAGGGGTACCGCGAGTGAATGGTGAGCAGCAT CACTGGGACATGTCGCTGCCTCTCATAGTGACACTAAGCACGATTTCGATCATCCTCCTAGCGGCCATGA TCACCATCGCTGTCAAATGTAAACGGGAAAACAAGGAGATTCGCACTTACAACTGCCGCATCGCCGAGTA CAGCCACCCACAACTAGGCGGGGGGAAGGGCAAGAAGAAGAAAATCAACAAAAACGATATCATGCTGGTG CAGAGCGAGGTGGAAGAGAGGAACGCCATGAACGTCATGAACGTGGTGAGCAGTCCCTCCCTGGCCACCT CCCCTATGTACTTCGACTACCAAACCCGCCTGCCCCTCAGCTCGCCCCGGTCAGAGGTGATGTATCTTAA GCCAGCCTCCAACAACCTGACTGTCCCGCAGGGGCACGCAGGCTGCCACAGCAGCTTCACCGGACAAGGG ACCAATTCGAGCGAGACCCCTGCCACTCGGATGTCCATAATTCAGACAGACAATTTTCCCGCAGAGCCCA ATTACATGGGCAGCAGGCAGCAGTTTGTTCAAAGTAGCTCCACGTTTAAAGACCCAGAAAGAGCCAGCCT GAGAGACAGTGGGCACGGGGACAGCGATCAGGCGGACAGTGACCAAGACACTAACAAAGGCTCCTGCTGT GACATGTCTGTTAGGGAGGCACTCAAGATGAAAACTACTTCAACTAAAAGTCAACCACTTGAACAAGAAC CGGAAGAGTGTATTAATTGCACAGATGAATGCCGAGTGCTTGGTCATTCTGATAGATGTTGGATGCCACA GTTCCCTGCAGCCAATCAGGCTGAAAATGCAGATTACCGCACAAATCTCTTTGTACCCACAGTTGAAGCT AACGTTGAGACTGAGACTTACGAAACTGTGAATCCCACTGGGAAAAAGACTTTTTGTACATTTGGAAAAG ACAAGCGAGAGCACACTATTCTCATTGCCAATGTGAAACCTTATTTAAAAGCCAAACGTGCCCTGAGCCC TCTCCTCCAAGAGGTCCCCTCAGCATCTAGCAGCCCAACCAAGGCATGCATTGAGCCTTGCGCCTCAACA AAAGGCTCCCTGGATGGCTGTGAAGCAAAAGCAGGGGCCTTAGCAGAAGCAAGCAGCTCCTACCTGCCCA CTGACAGTCAGTATCCGTCACCCAGTAAGCAACCAAGAGACCCTTCCTTCATGGCTTCTGAGCAGATGGC AAGGGTCTTTGCGGATGTGCACTCCAGAGCTAGTAGGGATTCCAGCGAGATGGGAGCTGTGCTGGAACAG TTGGATCACCCCAACAGAGATCTGGGCAGAGAGTCCGTGGATGCGGAGGAAGTTGTGAGAGAGATTGATA AGCTCTTGCAGGACTGCCGGGGAAATGACCCTGTGGCTGTGAGAAAAAATAACAATGGTTTTGACCAAAC TTATATTAGGACAGAATTAATGAAGCTTAAGGAGAAAGGAGAAAAGAAAGAGAGAGAGAGAGAGAGAGAA GAAATTGGAGAGAAGAAAAAGGAGGATGAGACAAGTTACCTTTTGACAATCTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210629 representing NM_001107279 Red=Cloning site Green=Tags(s) MYLSICCCFLLWAPALTLKNLNYSVPEEQGAGTVIGNIGKDARLQPGLPPAERGSGSGRSKSGSYRVLEN SAPHLLDVDADSGLLYTKQRIDRESLCRHNAKCQLSLEVFANDKEICMIKVEIQDINDNAPSFPSDQIEM DISENAAPGTRFPLTSAHDPDAGENGLRTYLLTRDDHGLFALDVKSRGDGTKFPELVIQKALDRELQNHH TLVLTALDGGEPPRSATVQINVKVIDSNDNSPVFEAPSYLVELPENAPLGTVVIDLNATDADEGPNGEVL YSFSSYVPDRVRELFSIDPKTGLIRVKGNLDYEENGMLEIDVQARDLGPNPIPAHCKVTVKLIDRNDNAP SIGFVSVRQGALSEAAPPGTVIALVRVTDRDSGKNGQLQCRVLGGGGTGGGGGLGGPGSVPFKLEENYDN FYTVVTDRPLDRETQDEYNVTIVARDGGSPPLNSTKSFAVKILDENDNPPRFTKGLYVLQVHENNIPGEY LGSVLAQDPDLGQNGTVSYSILPSHIGDVSIYTYVSVNPTNGAIYALRSFNYEQTKAFEFKVLAKDSGAP AHLESNATVRVTVLDVNDNAPVIVLPTLQNDTAELQVPRNAGLGYLVSTVRALDSDFGESGRLTYEIVDG NDDHLFEIDPSSGEIRTLHPFWEDVTPVVELVVKVTDHGKPTLSAVAKLIIRSVSGSLPEGVPRVNGEQH HWDMSLPLIVTLSTISIILLAAMITIAVKCKRENKEIRTYNCRIAEYSHPQLGGGKGKKKKINKNDIMLV QSEVEERNAMNVMNVVSSPSLATSPMYFDYQTRLPLSSPRSEVMYLKPASNNLTVPQGHAGCHSSFTGQG TNSSETPATRMSIIQTDNFPAEPNYMGSRQQFVQSSSTFKDPERASLRDSGHGDSDQADSDQDTNKGSCC DMSVREALKMKTTSTKSQPLEQEPEECINCTDECRVLGHSDRCWMPQFPAANQAENADYRTNLFVPTVEA NVETETYETVNPTGKKTFCTFGKDKREHTILIANVKPYLKAKRALSPLLQEVPSASSSPTKACIEPCAST KGSLDGCEAKAGALAEASSSYLPTDSQYPSPSKQPRDPSFMASEQMARVFADVHSRASRDSSEMGAVLEQ LDHPNRDLGRESVDAEEVVREIDKLLQDCRGNDPVAVRKNNNGFDQTYIRTELMKLKEKGEKKERERERE EIGEKKKEDETSYLLTIC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107279
ORF Size	3624 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107279.1, NP_001100749.1
RefSeq Size	4661 bp
RefSeq ORF	3627 bp
Locus ID	306055
MW	132.1 kDa

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