Serpina1 (NM_022519) Rat Tagged ORF Clone

CAT#: RR210158

  • TrueORF®

Serpina1 (Myc-DDK-tagged ORF) - Rat serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (Serpina1), (10 ug)



  "NM_022519" in other vectors (3)

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CNY 3,990.00


货期*
4周

规格
    • 10 ug

Cited in 1 publication.

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经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms AAT; Pi; Spi1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR210158 representing NM_022519
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCACCCTCCATCTCACGGGGGCTCCTGCTTCTGGCAGGCCTGTGTTGCCTGGCCCCCAGCTTCCTGG
CTGAGGATGCCCAGGAAACCGATACCTCCCAGCAGGACCAGAGTCCAACCTACCGTAAGATTTCTTCAAA
CCTGGCAGACTTTGCCTTCAGCCTATACCGGGAGCTGGTCCATCAATCCAATACATCCAACATCTTCTTC
TCCCCTATGAGCATCACCACAGCCTTCGCCATGCTCTCCCTGGGGAGCAAGGGTGACACTCGCAAACAGA
TTCTAGAGGGCCTGGAGTTCAACCTCACACAGATACCTGAGGCTGACATCCACAAGGCCTTCCATCACCT
CCTCCAAACTCTCAACAGGCCAGACAGTGAGCTGCAGCTGAACACAGGCAATGGCCTCTTTGTCAACAAG
AATCTGAAGCTGGTGGAGAAGTTTCTGGAAGAGGTCAAGAACAATTACCACTCAGAAGCCTTCTCTGTCA
ACTTTGCCGACTCAGAAGAGGCTAAGAAAGTAATTAATGATTATGTAGAGAAGGGAACCCAAGGAAAGAT
AGTTGATTTGATGAAACAGCTGGACGAAGACACGGTTTTTGCCCTGGTGAATTACATTTTCTTTAAAGGC
AAGTGGAAGAGGCCATTCAATCCTGAGCACACTAGGGATGCTGACTTCCACGTAGACAAGTCCACCACGG
TGAAGGTGCCCATGATGAACCGCCTGGGCATGTTTGACATGCACTATTGCAGCACACTGTCCAGCTGGGT
GCTGATGATGGATTACCTGGGCAACGCCACTGCCATCTTCCTCCTGCCCGATGATGGCAAGATGCAGCAT
CTGGAGCAAACTCTCACCAAGGATCTCATTTCCCGGTTCCTGCTAAACAGGCAAACAAGGTCAGCCATTC
TCTACTTCCCCAAACTGTCCATCTCTGGAACCTATAACTTGAAGACACTCCTGAGCTCACTGGGCATCAC
CCGGGTCTTCAACAATGATGCTGATCTCTCTGGAATCACAGAGGATGCCCCCCTGAAGCTTAGCCAGGCT
GTGCATAAGGCTGTGCTGACCTTAGATGAGAGGGGAACAGAGGCTGCAGGAGCCACTGTGGTGGAGGCCG
TCCCCATGTCTCTGCCCCCTCAAGTGAAGTTCGACCACCCTTTCATTTTCATGATAGTTGAATCAGAAAC
TCAGAGCCCCCTCTTTGTGGGAAAAGTGATAGATCCCACACGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR210158 representing NM_022519
Red=Cloning site Green=Tags(s)

MAPSISRGLLLLAGLCCLAPSFLAEDAQETDTSQQDQSPTYRKISSNLADFAFSLYRELVHQSNTSNIFF
SPMSITTAFAMLSLGSKGDTRKQILEGLEFNLTQIPEADIHKAFHHLLQTLNRPDSELQLNTGNGLFVNK
NLKLVEKFLEEVKNNYHSEAFSVNFADSEEAKKVINDYVEKGTQGKIVDLMKQLDEDTVFALVNYIFFKG
KWKRPFNPEHTRDADFHVDKSTTVKVPMMNRLGMFDMHYCSTLSSWVLMMDYLGNATAIFLLPDDGKMQH
LEQTLTKDLISRFLLNRQTRSAILYFPKLSISGTYNLKTLLSSLGITRVFNNDADLSGITEDAPLKLSQA
VHKAVLTLDERGTEAAGATVVEAVPMSLPPQVKFDHPFIFMIVESETQSPLFVGKVIDPTR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_022519
ORF Size 1233 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_022519.2, NP_071964.2
RefSeq Size 1603 bp
RefSeq ORF 1236 bp
Locus ID 24648
UniProt ID P17475
MW 46.1 kDa
Gene Summary Inhibitor of serine proteases. The primary target is elastase, but also has a moderate affinity for plasmin and thrombin.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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