Cpsf1 (NM_001130571) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MGC188689
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR206041 representing NM_001130571 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACGCGGTGTACAAGCAGGCGCACCCGCCCACCGGGCTCGAGTTTGCCATGTACTGCAACTTCTTCA ACAACAGTGAGCGCAACCTCGTGGTGGCCGGCACCTCGCAGCTCTATGTGTACCGCCTGAACCGCGACGC TGAGGCTCTGACCAAGAATGATGGGAGCACAGAGGGAAAGGCCCATCGAGAGAAACTAGAGCTGGTAGCC TCCTTCTCTTTTTTTGGCAATGTCATGTCCATGGCCAGCGTGCAGCTCGCAGGTGCCAAACGCGATGCTT TGCTTCTCAGCTTCAAAGACGCTAAGCTGTCTGTGGTGGAGTACGACCCAGGCACTCATGACCTGAAGAC CCTGTCTCTGCACTACTTTGAGGAGCCTGAACTTCGGGATGGATTTGTGCAGAACGTGCATACGCCCCGT GTGCGGGTGGACCCTGACGGGCGCTGTGCGGCAATGCTCATCTATGGCACAAGACTGGTAGTTCTGCCTT TCCGCAGAGAGAGCCTGGCTGAGGAGCATGAAGGGCTCATGGGTGAGGGGCAGAGGTCCAGCTTCCTGCC CAGCTACATCATCGATGTGCGGGCTCTGGATGAGAAGCTGCTCAACATCATTGACCTGCAGTTCCTACAC GGCTACTATGAGCCCACCCTGCTTATCCTGTTTGAGCCCAACCAGACCTGGCCAGGGCGGGTGGCTGTGA GGCAGGACACGTGCTCCATTGTGGCTATCTCGCTGAACATCACACAGAAAGTCCATCCAGTCATCTGGTC CCTCACCAGCTTGCCTTTTGACTGTACCCAGGCCCTGGCTGTGCCCAAGCCCATAGGTGGGGTAGTGATC TTCGCTGTCAACTCCCTGTTGTACCTGAACCAGAGTGTTCCCCCATATGGCGTGGCTCTCAACAGCCTTA CCACGGGCACCACTGCTTTTCCATTACGTACCCAAGAAGGTGTAAGGATCACCCTGGACTGTGCACAGGC GGCCTTCATCTCTTATGACAAGATGGTCATCTCCCTCAAGGGTGGTGAGATTTATGTGCTGACCCTCATC ACTGACGGCATGCGAAGTGTCCGAGCATTTCACTTTGACAAGGCAGCTGCTAGTGTCCTCACCACCAGCA TGGTCACAATGGAGCCTGGATACCTGTTCCTAGGCTCTCGCCTGGGCAATTCCCTCCTCCTCAAGTACAC GGAGAAGCTGCAGGAACCCCCAGCCAGCTCTGTTCGTGAGGCTGCTGACAAGGAAGAACCTCCCTCAAAG AAGAAGCGAGTGGACCCTACAGTGGGCTGGACAGGAGGCAAGACACAGGATGAGGTGGATGAAATTGAAG TTTATGGCAGTGAGGCCCAGTCAGGCACACAGTTAGCTACTTACTCTTTTGAGGTATGTGATAGCATGCT CAACATTGGACCCTGTGCCAATGCTGCTGTGGGCGAGCCTGCCTTCCTCTCCGAAGAGAACAGCCCTGAG CCAGACCTGGAGATTGTGGTTTGTTCCGGCTACGGGAAAAACGGGGCCCTGTCTGTGTTGCAGAAGAGTA TCCGGCCCCAGGTGGTGACAACCTTTGAACTTCCTGGATGCTATGATATGTGGACAGTCATTGCTCCTGT GCGGAAAGAGGAGGAAGAAACACCCAAAGCAGAGAGCACAGAGCAGGAACCTAGTGCCCCTAAAGCAGAG GAGGATGGGCGAAGACATGGATTCCTTATTCTTAGCCGAGAAGACTCAACCATGATCCTACAGACTGGCC AGGAGATCATGGAGCTGGACACCAGTGGCTTTGCCACACAGGGTCCTACTGTCTTTGCTGGAAACATTGG GGACAATCGTTATATTGTCCAAGTCTCGCCATTGGGCATTCGCCTGTTGGAAGGTGTGAATCAGCTGCAC TTCATCCCTGTGGACCTGGGCGCCCCCATCGTGCAGTGTGCTGTGGCTGATCCCTATGTGGTCATCATGA GTGCTGAGGGACACGTTACCATGTTCCTACTCAAGAGCGATTCCTATGGTGGCCGCCACCACCGGCTGGC ACTACACAAGCCTCCACTACATCATCAGTCTAAGGTGATTGCGCTCTGCCTGTACCGAGATGTCAGTGGC ATGTTCACCACTGAGAGCCGCCTGGGTGGGGCCCGTGATGAACTGGGTGGCCGAAGTGGCTCAGAGGCTG AAGGCCTGGGCTCAGAAACAAGCCCCACAGTGGACGATGAGGAAGAGATGCTATATGGGGACTCCAGTTC CCTCTTCAGTCCCAGCAAAGAGGAGGCCCGAAGGAGCAACCAGCCACCAGCTGATCGTGACCCTGCACCC TTCAGGGCTGACCCCACTCACTGGTGCCTGCTGGTGCGGGAGAATGGCACCATGGAGATCTACCAGCTCC CAGACTGGCGGCTGGTATTCCTAGTTAAGAACTTCCCTGTGGGACAGCGGGTTCTGGTGGATAGTTCATT TGGACAGCCCACTACACAAGGTGAAGTCCGAAAGGAGGAGGCCACACGCCAGGGGGAGCTGCCTTTGGTC AAGGAGGTGTTATTGGTTGCTCTGGGAAGCCGGCAGAGCAGGCCCTACCTGCTGGTACATGTGGACCAGG AGTTGCTTATCTATGAAGCCTTTCCCCATGACTCTCAGCTTGGCCAGGGAAACCTTAAAGTCCGTTTCAA GAAGGTCCCCCACAACATCAACTTCCGTGAAAAGAAGCCAAAGCCATCCAAGAAGAAGGCAGAAGGTTGC AGCACTGAGGAGGGATCTGGAGTCCGGGGCCGTGTGGCACGTTTCCGCTACTTTGAGGACATTTATGGCT ATTCGGGGGTCTTTATCTGTGGACCCTCACCTCACTGGCTCCTGGTAACTGGCCGTGGGGCTCTGCGTCT GCACCCCATGGGCATTGATGGCCCCATCGACTCCTTTGCTCCATTCCACAACGTGAACTGCCCCCGTGGT TTCCTGTACTTCAACAGACAGGGAGAGCTGAGGATCAGTGTCCTGCCTGCCTACCTGTCCTATGATGCCC CATGGCCGGTCAGGAAGATTCCACTCCGATGCACAGCCCACTATGTAGCCTACCACGTGGAATCCAAGGT ATATGCTGTGGCCACCAGCACCAACACACCTTGCACTAGAATCCCACGCATGACTGGTGAGGAGAAAGAA TTTGAGGCCATTGAGAGAGATGACAGATACATCCACCCCCAGCAGGAAGCCTTCTCCATCCAGCTCATCT CCCCAGTCAGCTGGGAGGCCATCCCCAATGCCAGGATCGAGCTAGAGGAATGGGAGCATGTCACGTGCAT GAAGACAGTGTCACTGCGCAGTGAGGAGACCGTGTCAGGCCTAAAGGGCTATGTGGCTGCTGGGACCTGC CTCATGCAGGGGGAGGAGGTCACGTGCCGTGGGCGGATCTTCCTGTGGAGCCTGCGGGCTAGCGAGCTGA CTGGCATGGCCTTCATCGACACCCAGCTGTACATCCACCAGATGATCAGCGTCAAGAACTTCATCCTGGC AGCAGACGTCATGAAGAGCATCTCACTGCTGCGCTACCAGGAGGAGAGCAAAACGCTCAGCCTGGTGTCC CGGGATGCCAAGCCTTTGGAGGTATACAGCGTGGACTTCATGGTGGACAATGCCCAGCTGGGCTTCTTGG TGTCTGACCGAGACCGCAATCTTATGGTGTATATGTATCTGCCAGAAGCCAAAGAGAGTTTTGGGGGCAT GCGCCTGCTGCGCCGAGCAGACTTCCATGTGGGTGCCCACGTGAACACGTTCTGGAGGACCCCATGCCGA GGTGCTGCTGAGGGGCCCAGCAAGAAGTCGGTCATGTGGGAAAACAAGCATATCACATGGTTTGCAACCC TTGATGGTGGCATTGGGCTCCTGCTGCCCATGCAAGAGAAGACATACAGACGACTGCTGATGCTGCAGAA TGCACTGACCACCATGCTACCCCACCACGCTGGCCTCAACCCCCGAGCCTTCCGAATGCTGCATGTAGAC CGGCGCATCCTGCAGAATGCTGTACGCAACGTCCTCGATGGAGAACTGCTGAACCGTTACCTATACCTCA GCACCATGGAGCGCAGTGAGCTGGCCAAGAAGATAGGCACCACACCTGACATCATCCTGGACGACTTGCT GGAGACAGACCGCGTCACGGCTCACTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR206041 representing NM_001130571 Red=Cloning site Green=Tags(s) MYAVYKQAHPPTGLEFAMYCNFFNNSERNLVVAGTSQLYVYRLNRDAEALTKNDGSTEGKAHREKLELVA SFSFFGNVMSMASVQLAGAKRDALLLSFKDAKLSVVEYDPGTHDLKTLSLHYFEEPELRDGFVQNVHTPR VRVDPDGRCAAMLIYGTRLVVLPFRRESLAEEHEGLMGEGQRSSFLPSYIIDVRALDEKLLNIIDLQFLH GYYEPTLLILFEPNQTWPGRVAVRQDTCSIVAISLNITQKVHPVIWSLTSLPFDCTQALAVPKPIGGVVI FAVNSLLYLNQSVPPYGVALNSLTTGTTAFPLRTQEGVRITLDCAQAAFISYDKMVISLKGGEIYVLTLI TDGMRSVRAFHFDKAAASVLTTSMVTMEPGYLFLGSRLGNSLLLKYTEKLQEPPASSVREAADKEEPPSK KKRVDPTVGWTGGKTQDEVDEIEVYGSEAQSGTQLATYSFEVCDSMLNIGPCANAAVGEPAFLSEENSPE PDLEIVVCSGYGKNGALSVLQKSIRPQVVTTFELPGCYDMWTVIAPVRKEEEETPKAESTEQEPSAPKAE EDGRRHGFLILSREDSTMILQTGQEIMELDTSGFATQGPTVFAGNIGDNRYIVQVSPLGIRLLEGVNQLH FIPVDLGAPIVQCAVADPYVVIMSAEGHVTMFLLKSDSYGGRHHRLALHKPPLHHQSKVIALCLYRDVSG MFTTESRLGGARDELGGRSGSEAEGLGSETSPTVDDEEEMLYGDSSSLFSPSKEEARRSNQPPADRDPAP FRADPTHWCLLVRENGTMEIYQLPDWRLVFLVKNFPVGQRVLVDSSFGQPTTQGEVRKEEATRQGELPLV KEVLLVALGSRQSRPYLLVHVDQELLIYEAFPHDSQLGQGNLKVRFKKVPHNINFREKKPKPSKKKAEGC STEEGSGVRGRVARFRYFEDIYGYSGVFICGPSPHWLLVTGRGALRLHPMGIDGPIDSFAPFHNVNCPRG FLYFNRQGELRISVLPAYLSYDAPWPVRKIPLRCTAHYVAYHVESKVYAVATSTNTPCTRIPRMTGEEKE FEAIERDDRYIHPQQEAFSIQLISPVSWEAIPNARIELEEWEHVTCMKTVSLRSEETVSGLKGYVAAGTC LMQGEEVTCRGRIFLWSLRASELTGMAFIDTQLYIHQMISVKNFILAADVMKSISLLRYQEESKTLSLVS RDAKPLEVYSVDFMVDNAQLGFLVSDRDRNLMVYMYLPEAKESFGGMRLLRRADFHVGAHVNTFWRTPCR GAAEGPSKKSVMWENKHITWFATLDGGIGLLLPMQEKTYRRLLMLQNALTTMLPHHAGLNPRAFRMLHVD RRILQNAVRNVLDGELLNRYLYLSTMERSELAKKIGTTPDIILDDLLETDRVTAHF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001130571
ORF Size	4158 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001130571.1, NP_001124043.1
RefSeq Size	4374 bp
RefSeq ORF	4161 bp
Locus ID	366952
MW	154.9 kDa

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