Atp7a (NM_052803) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Mnk
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR205912 representing NM_052803 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCAAATATGGATGCAAATTCAATTACTATCACTGTTGAGGGAATGACATGTATTTCCTGTGTCC GGACCATTGAGCAGCAGATTGGGAAAGTGAATGGTGTCCATCACATTAAAGTTTCCCTAGAAGAAAAAAG TGCAACTGTTATTTATAACCCTAAACTTCAGACTCCAAAGACCCTCCAAGAAGCTATCGATGACATGGGC TTTGATGCTCTTCTTCACAATGCTAACCCTCTTCCTGTCTTAACCAATACTGTGTTTCTGACTGTTACTG CTCCACTGGCTCTGCCATGGGACCATATCCAAAGTACATTGCTCAAGACCAAGGGTGTGACTGGGGTTAA GATTTCCCCTCAGCAAAGAAGTGCAGTGGTTACCATAATCCCATCTGTGGTGAGTGCTAATCAGATCGTG GAGCTGGTCCCAGACCTCAGTTTAGACATGGGAACTCAGGAGAAAAAGTCAGGAACTTCTGAAGAGCATA GCACACCTCAGGCAGGGGAAGTCCTGCTGAAGATGAGAGTGGAAGGGATGACCTGCCATTCATGCACTAG CACCATTGAAGGAAAAGTTGGAAAGCTGCAAGGTGTACAACGCATTAAAGTGTCCCTAGACAACCAAGAA GCTACTATTGTGTATCAACCTCATCTGATCACAGCAGAGGAAATAAAGAAGCAGATTGAAGCTGTGGGTT TTCCAGCCTTCATAAAAAAACAGCCAAAGTACCTCAAATTGGGAGCCATTGACGTTGAGCGCCTGAAGAG TACACCAGTCAAATCTTCAGAAGGATCTCAGCAAAAGAGCCCAGCGTATCCCAGTGACTCAGCAATCACA TTTACCATAGACGGCATGCATTGTAAATCATGTGTGTCAAATATCGAAAGTGCTTTATCTACACTCCAGT ATGTAAGCAGCATAGTAGTTTCTTTAGAGAATAGGTCAGCCATTGTAAAGTACAATGCAAGCTTAGTCAC TCCAGAAATACTGAGAAAAGCGATAGAGGCCGTTTCTCCAGGGCAATACAGAGTTAGTATTTCAAGTGAA GTTGAAAGTCCCACCAGCTCTCCCTCCAGCTCATCTCTTCAGAAGATGCCTTTGAACTTAGTGAGCCAGC CTCTGACCCAAGAAGTTGTAATCAACATTAATGGCATGACTTGTAATTCTTGTGTGCAGTCGATAGAGGG TGTAATATCGAAGAAGCCAGGTGTAAAATCCATCCACGTTTCCCTCACAAATAGCACCGGGACTATTGAA TATGATCCTCTACTAACCTCTCCAGAACCCTTGCGAGAAGCAATTGAAGACATGGGATTTGATGCTGTCC TTCCAGCAGACATGAAAGAGCCACTGGTCGTGATAGCTCAGCCCTCACTGGAAACACCTCTTTTGCCCTC AACTACTGAGCCAGAAAATGTGATGACACCAGTTCAGAACAAGTGTTACATACAGGTCTCGGGGATGACG TGTGCTTCTTGTGTAGCAAACATTGAACGGAACTTAAGACGAGAAGAAGGAATATATTCTGTACTTGTAG CCCTAATGGCTGGCAAGGCGGAAGTAAGATATAACCCAGCTGTTATCCAACCCCGAGTGATAGCAGAGCT TATTCGAGAGCTTGGCTTTGGAGCTGTGGTGATGGAAAATGCTGGAGAAGGCAATGGCATTTTGGAACTT GTTGTGAGAGGAATGACGTGTGCCTCCTGTGTCCATAAAATTGAATCAACGCTCACAAAACACAAAGGGA TCTTCTACTGCTCTGTGGCCCTGGCAACGAACAAAGCACATATTAAATATGATCCAGAAATTATTGGTCC CAGAGATATTATCCATACCATTGGAAACTTAGGTTTTGAAGCTTCTTTGGTCAAGAAAGATCGATCAGCC AACCACTTAGATCATAAACGAGAAATAAAACAATGGAGAGGGTCTTTCCTTGTGAGCCTGTTTTTCTGTA TCCCTGTAATGGGACTGATGATCTATATGATGGTTATGGACCACCACCTTGCAACTCTAAACCATAATCA GAACATGAGTAATGAAGAAATGATCAACATGCATTCTTCTATGTTCCTGGAGCGTCAGATCCTGCCGGGA CTGTCCATTATGAATTTGTTGTCCCTTTTATTGTGTCTACCTGTACAGTTTTGTGGAGGCTGGTACTTTT ACATTCAGGCTTACAAAGCGCTGAGGCATAAGACAGCAAATATGGATGTGCTGATTGTGTTGGCAACCAC CATTGCATTTGCCTACTCTCTGGTCATTCTTCTGGTTGCAATGTATGAGAGAGCCAAAGTGAACCCTATT ACCTTCTTTGATACACCTCCTATGCTGTTTGTGTTTATCGCACTAGGCCGATGGCTGGAACACATAGCGA AGGGCAAAACTTCCGAGGCTCTTGCAAAGCTAATTTCCTTACAAGCAACAGAAGCAACTATTGTAACTCT TAACTCTGAAAATCTCCTCCTGAGTGAAGAACAAGTGGATGTGGAACTTGTACAACGTGGAGATATCATT AAGGTCGTTCCAGGAGGCAAATTTCCAGTGGATGGCCGTGTTATTGAAGGGCATTCTATGGTAGACGAGT CCCTCATCACAGGGGAAGCAATGCCTGTGGCTAAGAAACCTGGCAGCACGGTGATTGCAGGTTCCATTAA CCAGAATGGATCGCTCCTCATCCGGGCAACACACGTAGGGGCAGATACAACACTTTCTCAAATTGTCAAA CTTGTAGAGGAGGCACAGACATCAAAGGCTCCTATCCAGCAGTTTGCAGACAAACTCAGTGGCTACTTTG TTCCTTTTATCGTCTTGGTTTCCATTGTTACCCTCTTGGTGTGGATTATAATTGGATTTCAAAATTTTGA AATTGTGGAAGCCTACTTTCCCGGCTACAACAGAAGCATCTCCCGAACAGAAACCATAATCCGCTTTGCT TTCCAAGCGTCTATCACAGTTCTGTGTATCGCATGTCCCTGTTCACTGGGGCTAGCCACTCCAACTGCTG TGATGGTGGGCACAGGAGTAGGTGCTCAGAATGGCATACTTATCAAAGGTGGGGAGCCACTGGAGATGGC TCATAAGGTAAAGGTAGTGGTGTTTGACAAGACTGGAACCATTACCCATGGAACCCCAGTAGTGAACCAA GTAAAGGTTCTGGTGGAAAGTAACAAGATATCACGCAATAAGATCCTGGCCATTGTGGGAACTGCAGAAA GTAACAGTGAACATCCTTTAGGAGCAGCTGTAACCAAATATTGCAAGCAGGAGCTGGACACTGAAACCTT GGGTACCTGCACAGATTTCCAGGTTGTACCCGGCTGTGGAATTAGCTGTAAAGTCACCAATATTGAAGGT TTGCTACATAAGAGTAACTTGAAGATAGAAGAAAATAACATTAAAAATGCATCCCTGGTTCAAATTGATG CAATTAATGAACAGTCATCACCTTCATCGTCTATGATTATTGATGCTCATCTCTCAAATGCTGTTAATAC TCAGCAGTACAAAGTCCTCATTGGTAACCGGGAATGGATGATTAGAAATGGTCTTGTCATAAGTAATGAT GTGGACGAGTCTATGATTGAACATGAAAGAAGAGGCCGGACTGCTGTGTTGGTGACAATCGATGATGAAC TGTGTGGCTTGATCGCTATTGCTGATACTGTGAAACCTGAGGCAGAGCTGGCTGTGCACATTCTGAAATC TATGGGTTTGGAAGTAGTTCTGATGACTGGAGACAACAGTAAAACAGCTCGGTCTATTGCTTCTCAGGTT GGCATTACTAAGGTGTTTGCTGAAGTTCTGCCTTCCCACAAAGTTGCTAAGGTGAAGCAGCTTCAGGAGG AGGGAAAGCGCGTAGCCATGGTAGGAGATGGAATCAATGACTCTCCAGCTCTGGCAATGGCAAGCGTCGG AATTGCCATCGGCACAGGCACAGATGTAGCCATCGAAGCAGCTGACGTGGTTTTGATAAGGAATGACCTT CTGGATGTTGTGGCAAGTATTGACTTGTCAAGGAAAACAGTCAAGAGGATTCGAATCAATTTTGTCTTTG CTCTGATTTATAATCTGATTGGAATTCCCATCGCTGCTGGAGTTTTTCTGCCCATCGGCTTGGTTTTACA ACCCTGGATGGGATCCGCAGCCATGGCCGCTTCATCTGTCTCTGTGGTACTTTCTTCCCTTTTCCTCAAG CTTTACAGGAAGCCAACATATGACAATTATGAGTTGCGTCCCCGGAGCCACACAGGACAGAGGAGTCCTT CAGAAATCAGCGTTCACGTTGGAATAGATGATACCTCCAGAAATTCTCCAAGACTGGGTTTACTGGACCG GATTGTCAATTACAGCAGAGCCTCCATAAATTCACTGCTGTCTGACAAACGCTCCCTCAACAGCGTCGTC ACTAGTGAGCCTGATAAGCACTCACTTCTGGTGGGAGACTTCCGGGAAGATGACGACACCACACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR205912 representing NM_052803 Red=Cloning site Green=Tags(s) MEPNMDANSITITVEGMTCISCVRTIEQQIGKVNGVHHIKVSLEEKSATVIYNPKLQTPKTLQEAIDDMG FDALLHNANPLPVLTNTVFLTVTAPLALPWDHIQSTLLKTKGVTGVKISPQQRSAVVTIIPSVVSANQIV ELVPDLSLDMGTQEKKSGTSEEHSTPQAGEVLLKMRVEGMTCHSCTSTIEGKVGKLQGVQRIKVSLDNQE ATIVYQPHLITAEEIKKQIEAVGFPAFIKKQPKYLKLGAIDVERLKSTPVKSSEGSQQKSPAYPSDSAIT FTIDGMHCKSCVSNIESALSTLQYVSSIVVSLENRSAIVKYNASLVTPEILRKAIEAVSPGQYRVSISSE VESPTSSPSSSSLQKMPLNLVSQPLTQEVVININGMTCNSCVQSIEGVISKKPGVKSIHVSLTNSTGTIE YDPLLTSPEPLREAIEDMGFDAVLPADMKEPLVVIAQPSLETPLLPSTTEPENVMTPVQNKCYIQVSGMT CASCVANIERNLRREEGIYSVLVALMAGKAEVRYNPAVIQPRVIAELIRELGFGAVVMENAGEGNGILEL VVRGMTCASCVHKIESTLTKHKGIFYCSVALATNKAHIKYDPEIIGPRDIIHTIGNLGFEASLVKKDRSA NHLDHKREIKQWRGSFLVSLFFCIPVMGLMIYMMVMDHHLATLNHNQNMSNEEMINMHSSMFLERQILPG LSIMNLLSLLLCLPVQFCGGWYFYIQAYKALRHKTANMDVLIVLATTIAFAYSLVILLVAMYERAKVNPI TFFDTPPMLFVFIALGRWLEHIAKGKTSEALAKLISLQATEATIVTLNSENLLLSEEQVDVELVQRGDII KVVPGGKFPVDGRVIEGHSMVDESLITGEAMPVAKKPGSTVIAGSINQNGSLLIRATHVGADTTLSQIVK LVEEAQTSKAPIQQFADKLSGYFVPFIVLVSIVTLLVWIIIGFQNFEIVEAYFPGYNRSISRTETIIRFA FQASITVLCIACPCSLGLATPTAVMVGTGVGAQNGILIKGGEPLEMAHKVKVVVFDKTGTITHGTPVVNQ VKVLVESNKISRNKILAIVGTAESNSEHPLGAAVTKYCKQELDTETLGTCTDFQVVPGCGISCKVTNIEG LLHKSNLKIEENNIKNASLVQIDAINEQSSPSSSMIIDAHLSNAVNTQQYKVLIGNREWMIRNGLVISND VDESMIEHERRGRTAVLVTIDDELCGLIAIADTVKPEAELAVHILKSMGLEVVLMTGDNSKTARSIASQV GITKVFAEVLPSHKVAKVKQLQEEGKRVAMVGDGINDSPALAMASVGIAIGTGTDVAIEAADVVLIRNDL LDVVASIDLSRKTVKRIRINFVFALIYNLIGIPIAAGVFLPIGLVLQPWMGSAAMAASSVSVVLSSLFLK LYRKPTYDNYELRPRSHTGQRSPSEISVHVGIDDTSRNSPRLGLLDRIVNYSRASINSLLSDKRSLNSVV TSEPDKHSLLVGDFREDDDTTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_052803
ORF Size	4476 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_052803.2, NP_434690.1
RefSeq Size	4677 bp
RefSeq ORF	4479 bp
Locus ID	24941
UniProt ID	P70705
MW	162.1 kDa
Gene Summary	This gene encodes a transmembrane protein that functions in copper transport across membranes. The protein localizes to the trans-Golgi network, where it is predicted to supply copper to copper-dependent enzymes in the secretory pathway. The protein relocalizes to the plasma membrane under conditions of elevated extracellular copper and functions in the efflux of copper from cells. In human, mutations in this gene result in Menkes disease, X-linked cutis laxa, and occipital horn syndrome. [provided by RefSeq, Nov 2009]

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