Atp7a (NM_052803) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Mnk
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205912 representing NM_052803 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCAAATATGGATGCAAATTCAATTACTATCACTGTTGAGGGAATGACATGTATTTCCTGTGTCC GGACCATTGAGCAGCAGATTGGGAAAGTGAATGGTGTCCATCACATTAAAGTTTCCCTAGAAGAAAAAAG TGCAACTGTTATTTATAACCCTAAACTTCAGACTCCAAAGACCCTCCAAGAAGCTATCGATGACATGGGC TTTGATGCTCTTCTTCACAATGCTAACCCTCTTCCTGTCTTAACCAATACTGTGTTTCTGACTGTTACTG CTCCACTGGCTCTGCCATGGGACCATATCCAAAGTACATTGCTCAAGACCAAGGGTGTGACTGGGGTTAA GATTTCCCCTCAGCAAAGAAGTGCAGTGGTTACCATAATCCCATCTGTGGTGAGTGCTAATCAGATCGTG GAGCTGGTCCCAGACCTCAGTTTAGACATGGGAACTCAGGAGAAAAAGTCAGGAACTTCTGAAGAGCATA GCACACCTCAGGCAGGGGAAGTCCTGCTGAAGATGAGAGTGGAAGGGATGACCTGCCATTCATGCACTAG CACCATTGAAGGAAAAGTTGGAAAGCTGCAAGGTGTACAACGCATTAAAGTGTCCCTAGACAACCAAGAA GCTACTATTGTGTATCAACCTCATCTGATCACAGCAGAGGAAATAAAGAAGCAGATTGAAGCTGTGGGTT TTCCAGCCTTCATAAAAAAACAGCCAAAGTACCTCAAATTGGGAGCCATTGACGTTGAGCGCCTGAAGAG TACACCAGTCAAATCTTCAGAAGGATCTCAGCAAAAGAGCCCAGCGTATCCCAGTGACTCAGCAATCACA TTTACCATAGACGGCATGCATTGTAAATCATGTGTGTCAAATATCGAAAGTGCTTTATCTACACTCCAGT ATGTAAGCAGCATAGTAGTTTCTTTAGAGAATAGGTCAGCCATTGTAAAGTACAATGCAAGCTTAGTCAC TCCAGAAATACTGAGAAAAGCGATAGAGGCCGTTTCTCCAGGGCAATACAGAGTTAGTATTTCAAGTGAA GTTGAAAGTCCCACCAGCTCTCCCTCCAGCTCATCTCTTCAGAAGATGCCTTTGAACTTAGTGAGCCAGC CTCTGACCCAAGAAGTTGTAATCAACATTAATGGCATGACTTGTAATTCTTGTGTGCAGTCGATAGAGGG TGTAATATCGAAGAAGCCAGGTGTAAAATCCATCCACGTTTCCCTCACAAATAGCACCGGGACTATTGAA TATGATCCTCTACTAACCTCTCCAGAACCCTTGCGAGAAGCAATTGAAGACATGGGATTTGATGCTGTCC TTCCAGCAGACATGAAAGAGCCACTGGTCGTGATAGCTCAGCCCTCACTGGAAACACCTCTTTTGCCCTC AACTACTGAGCCAGAAAATGTGATGACACCAGTTCAGAACAAGTGTTACATACAGGTCTCGGGGATGACG TGTGCTTCTTGTGTAGCAAACATTGAACGGAACTTAAGACGAGAAGAAGGAATATATTCTGTACTTGTAG CCCTAATGGCTGGCAAGGCGGAAGTAAGATATAACCCAGCTGTTATCCAACCCCGAGTGATAGCAGAGCT TATTCGAGAGCTTGGCTTTGGAGCTGTGGTGATGGAAAATGCTGGAGAAGGCAATGGCATTTTGGAACTT GTTGTGAGAGGAATGACGTGTGCCTCCTGTGTCCATAAAATTGAATCAACGCTCACAAAACACAAAGGGA TCTTCTACTGCTCTGTGGCCCTGGCAACGAACAAAGCACATATTAAATATGATCCAGAAATTATTGGTCC CAGAGATATTATCCATACCATTGGAAACTTAGGTTTTGAAGCTTCTTTGGTCAAGAAAGATCGATCAGCC AACCACTTAGATCATAAACGAGAAATAAAACAATGGAGAGGGTCTTTCCTTGTGAGCCTGTTTTTCTGTA TCCCTGTAATGGGACTGATGATCTATATGATGGTTATGGACCACCACCTTGCAACTCTAAACCATAATCA GAACATGAGTAATGAAGAAATGATCAACATGCATTCTTCTATGTTCCTGGAGCGTCAGATCCTGCCGGGA CTGTCCATTATGAATTTGTTGTCCCTTTTATTGTGTCTACCTGTACAGTTTTGTGGAGGCTGGTACTTTT ACATTCAGGCTTACAAAGCGCTGAGGCATAAGACAGCAAATATGGATGTGCTGATTGTGTTGGCAACCAC CATTGCATTTGCCTACTCTCTGGTCATTCTTCTGGTTGCAATGTATGAGAGAGCCAAAGTGAACCCTATT ACCTTCTTTGATACACCTCCTATGCTGTTTGTGTTTATCGCACTAGGCCGATGGCTGGAACACATAGCGA AGGGCAAAACTTCCGAGGCTCTTGCAAAGCTAATTTCCTTACAAGCAACAGAAGCAACTATTGTAACTCT TAACTCTGAAAATCTCCTCCTGAGTGAAGAACAAGTGGATGTGGAACTTGTACAACGTGGAGATATCATT AAGGTCGTTCCAGGAGGCAAATTTCCAGTGGATGGCCGTGTTATTGAAGGGCATTCTATGGTAGACGAGT CCCTCATCACAGGGGAAGCAATGCCTGTGGCTAAGAAACCTGGCAGCACGGTGATTGCAGGTTCCATTAA CCAGAATGGATCGCTCCTCATCCGGGCAACACACGTAGGGGCAGATACAACACTTTCTCAAATTGTCAAA CTTGTAGAGGAGGCACAGACATCAAAGGCTCCTATCCAGCAGTTTGCAGACAAACTCAGTGGCTACTTTG TTCCTTTTATCGTCTTGGTTTCCATTGTTACCCTCTTGGTGTGGATTATAATTGGATTTCAAAATTTTGA AATTGTGGAAGCCTACTTTCCCGGCTACAACAGAAGCATCTCCCGAACAGAAACCATAATCCGCTTTGCT TTCCAAGCGTCTATCACAGTTCTGTGTATCGCATGTCCCTGTTCACTGGGGCTAGCCACTCCAACTGCTG TGATGGTGGGCACAGGAGTAGGTGCTCAGAATGGCATACTTATCAAAGGTGGGGAGCCACTGGAGATGGC TCATAAGGTAAAGGTAGTGGTGTTTGACAAGACTGGAACCATTACCCATGGAACCCCAGTAGTGAACCAA GTAAAGGTTCTGGTGGAAAGTAACAAGATATCACGCAATAAGATCCTGGCCATTGTGGGAACTGCAGAAA GTAACAGTGAACATCCTTTAGGAGCAGCTGTAACCAAATATTGCAAGCAGGAGCTGGACACTGAAACCTT GGGTACCTGCACAGATTTCCAGGTTGTACCCGGCTGTGGAATTAGCTGTAAAGTCACCAATATTGAAGGT TTGCTACATAAGAGTAACTTGAAGATAGAAGAAAATAACATTAAAAATGCATCCCTGGTTCAAATTGATG CAATTAATGAACAGTCATCACCTTCATCGTCTATGATTATTGATGCTCATCTCTCAAATGCTGTTAATAC TCAGCAGTACAAAGTCCTCATTGGTAACCGGGAATGGATGATTAGAAATGGTCTTGTCATAAGTAATGAT GTGGACGAGTCTATGATTGAACATGAAAGAAGAGGCCGGACTGCTGTGTTGGTGACAATCGATGATGAAC TGTGTGGCTTGATCGCTATTGCTGATACTGTGAAACCTGAGGCAGAGCTGGCTGTGCACATTCTGAAATC TATGGGTTTGGAAGTAGTTCTGATGACTGGAGACAACAGTAAAACAGCTCGGTCTATTGCTTCTCAGGTT GGCATTACTAAGGTGTTTGCTGAAGTTCTGCCTTCCCACAAAGTTGCTAAGGTGAAGCAGCTTCAGGAGG AGGGAAAGCGCGTAGCCATGGTAGGAGATGGAATCAATGACTCTCCAGCTCTGGCAATGGCAAGCGTCGG AATTGCCATCGGCACAGGCACAGATGTAGCCATCGAAGCAGCTGACGTGGTTTTGATAAGGAATGACCTT CTGGATGTTGTGGCAAGTATTGACTTGTCAAGGAAAACAGTCAAGAGGATTCGAATCAATTTTGTCTTTG CTCTGATTTATAATCTGATTGGAATTCCCATCGCTGCTGGAGTTTTTCTGCCCATCGGCTTGGTTTTACA ACCCTGGATGGGATCCGCAGCCATGGCCGCTTCATCTGTCTCTGTGGTACTTTCTTCCCTTTTCCTCAAG CTTTACAGGAAGCCAACATATGACAATTATGAGTTGCGTCCCCGGAGCCACACAGGACAGAGGAGTCCTT CAGAAATCAGCGTTCACGTTGGAATAGATGATACCTCCAGAAATTCTCCAAGACTGGGTTTACTGGACCG GATTGTCAATTACAGCAGAGCCTCCATAAATTCACTGCTGTCTGACAAACGCTCCCTCAACAGCGTCGTC ACTAGTGAGCCTGATAAGCACTCACTTCTGGTGGGAGACTTCCGGGAAGATGACGACACCACACTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_052803
Insert Size	4479 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_052803.2, NP_434690.1
RefSeq Size	4677 bp
RefSeq ORF	4479 bp
Locus ID	24941
UniProt ID	P70705
Gene Summary	This gene encodes a transmembrane protein that functions in copper transport across membranes. The protein localizes to the trans-Golgi network, where it is predicted to supply copper to copper-dependent enzymes in the secretory pathway. The protein relocalizes to the plasma membrane under conditions of elevated extracellular copper and functions in the efflux of copper from cells. In human, mutations in this gene result in Menkes disease, X-linked cutis laxa, and occipital horn syndrome. [provided by RefSeq, Nov 2009]

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