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Mgat4a (NM_001012225) Rat Tagged ORF Clone
CAT＃: RR204536
- TrueORF®
Mgat4a (Myc-DDK-tagged ORF) - Rat mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A (Mgat4a), (10 ug)
"NM_001012225" in other vectors (3)
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CNY 6,750.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR204536 representing NM_001012225
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCTCCGAAATGGAACTGTGGCCACTGCGCTGGTGTTTGTCACGTCCTTCCTTACCCTGTCCTGGT ATACCACATGGCAAAATGGGAAAGAAAAATTAATTGCTTACCAACGAGAATTCCTCGCTTTAAAAGAACG TCTTCGAGTGGCCGAACATAGGATATCTCAGCGCTCCTCTGAGCTAAACACCATTGTCCAACAGTTCCGC CGAGCTGGAGCGGAGACTAATGGAAATAATACCATAAAGCTTCTAAAAGAGTTGACAAGCAAAAAATCAC TTCAAGTGCCAAGTATTTATTATCATTTGCCTCATCTATTGCAAAATGAAAGAAGCCTTCAGCCCGCCGT ACAGATTGGCAGTGGAAGAACGGGAGTTTCAATAGTCATGGGGATTCCTACTGTGAAGAGAGAAGTTAAA TCTTACCTCATAGAGACCCTTCACTCCCTTATTGATAATCTGTATCCTGAAGAGAAGCTGGACTGTGTTA TAGTTGTCTTCATAGGAGAGACAGATCTTGATTATGTTCACAGCGTTGTTGCCAACCTGGAGAAAGAATT TTCTAGAGAAATTAGTTCCGGCTTGCTAGAAGTAATCTCTCCTCCTGAAAGCTATTACCCCGACTTAACA AACCTGAAGGAGACGTTCGGAGACTCCAAGGAAAGAGTAAGATGGAGAACGAAGCAAAACCTGGACTATT GTTTTCTGATGATGTATGCTCAGGAGAAGGGCATTTACTACATTCAGCTTGAAGATGATATTATTGTCAA GCAAAACTATTTTAATACCATAAAAAATTTTGCACTTCAACTTTCTTCTGAAGAATGGATGATTCTAGAG TTTTCCCAGCTGGGCTTCATTGGGAAGATGTTCCAGGCGCCGGACCTAGCTCTGGTTGTGGAGTTCATTC TCATGTTCTATAAGGAGAAGCCCATTGACTGGCTCTTGGACCACATTCTCTGGGTGAAGGTCTGCAACCC CGAAAAAGATGCCAAACACTGCGACAGACAGAAGGCAAACCTACGAATCCGCTTCCGACCCTCCCTCTTC CAGCACGTGGGTCTACACTCATCTCTGTCGGGGAAAATTCAGAAACTTACGGATAAAGATTACATGAAAC CATTGCTTCTCAAGATCCACGTGAACCCGCCTGCAGAGGTCTCCACTTCCCTGAAGGTGTACCAAGGGCA CACACTGGAGAAGACCTACATGGGGGAAGACTTCTTTTGGGCCATCACCCCCACAGCTGGAGACTACATC TTGTTTAAATTTGATAAACCGGTCAATGTGGAGAGTTATTTGTTCCACAGCGGCAATCAAGAGCACCCAG GGGACATCCTGCTGAACACGACCGTGGAGGTTCTGCCTCTTAAGAGCGACAGTTTGGAGATCAGCAAAGA AACCAAAGACAAACGATTAGAAGATGGCTATTTCAGAATAGGGAAATTTGAGTACGGAGTTGCAGAGGGA ATTGTGGATCCTGGACTGAACCCTATCTCAGCCTTTCGACTGTCGGTTATTCAGAACTCTGCTGTCTGGG CCATTCTCAATGAGATTCATATTAAGAAAGTCACCAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204536 representing NM_001012225
Red=Cloning site Green=Tags(s) MRLRNGTVATALVFVTSFLTLSWYTTWQNGKEKLIAYQREFLALKERLRVAEHRISQRSSELNTIVQQFR RAGAETNGNNTIKLLKELTSKKSLQVPSIYYHLPHLLQNERSLQPAVQIGSGRTGVSIVMGIPTVKREVK SYLIETLHSLIDNLYPEEKLDCVIVVFIGETDLDYVHSVVANLEKEFSREISSGLLEVISPPESYYPDLT NLKETFGDSKERVRWRTKQNLDYCFLMMYAQEKGIYYIQLEDDIIVKQNYFNTIKNFALQLSSEEWMILE FSQLGFIGKMFQAPDLALVVEFILMFYKEKPIDWLLDHILWVKVCNPEKDAKHCDRQKANLRIRFRPSLF QHVGLHSSLSGKIQKLTDKDYMKPLLLKIHVNPPAEVSTSLKVYQGHTLEKTYMGEDFFWAITPTAGDYI LFKFDKPVNVESYLFHSGNQEHPGDILLNTTVEVLPLKSDSLEISKETKDKRLEDGYFRIGKFEYGVAEG IVDPGLNPISAFRLSVIQNSAVWAILNEIHIKKVTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001012225 |
| ORF Size | 1578 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001012225.2, NP_001012225.1 |
| RefSeq Size | 7181 bp |
| RefSeq ORF | 1581 bp |
| Locus ID | 367252 |
| UniProt ID | Q5M854 |
| MW | 60.6 kDa |
| Gene Summary | Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN204536 | Mgat4a (untagged ORF) - Rat mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A (Mgat4a), (10 ug) |
CNY 6,750.00 |
|
| RR204536L3 | Lenti ORF clone of Mgat4a (Myc-DDK-tagged ORF) - Rat mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A (Mgat4a), (10 ug) |
CNY 8,650.00 |
|
| RR204536L4 | Lenti ORF clone of Mgat4a (mGFP-tagged ORF) - Rat mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A (Mgat4a), (10 ug) |
CNY 9,500.00 |
