Mrc1 (NM_001106123) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR203299 representing NM_001106123 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGACTCCCCCTGCTCCTGGCTTTTATCTCTGTGATTCCGGTCGCTGTTCAACTCTTGGACTCACGGC AATTTTTAATCTATAATGAGGACCACAAGCGCTGCGTGGACGCTCTAAGCGCCATCTCCGTTCAGACGGC AACTTGCAACCCGGAAGCTGAATCCCAGAAATTCCGCTGGGTGTCGGAGTCGCAGATCATGAGTGTTGCT TTCAAATTGTGTTTGGGGGTGCCATCGAAAACTGACTGGGCCTCCGTCACCCTGTATGCCTGCGACTCGA AAAGTGAATTTCAGAAATGGGAGTGTAAAAACGACACACTCTTTGGAATCAAGGGCACAGAGCTATATTT TAACTATGGCAACAGGCAAGAAAAGAATATCAAGCTTTACAAAGGTTCCGGTTTGTGGAGCAGATGGAAG GTCTACGGGACCACAGATGACCTGTGCTCTAGGGGATATGAAGCCATGTACTCCTTACTGGGCAATGCAA ACGGAGCTGTCTGTGCATTTCCATTCAAGTTTGAAAACAAGTGGTACGCAGACTGCACCTCTGCCGGGCG GTCAGATGGATGGCTCTGGTGTGGAACCACCACTGACTATGACACCGACAAGCTGTTTGGATTTTGTCCA CTGCAGTTTGAGGGCAGCAAGAGGCTGTGGAATACAGACCCACTGACTGGCATTCTTTACCAGATAAACT CCAAGTCTGCCTTAACCTGGCACCAGGCAAGAGCAAGCTGCAAGCAGCAGAATGCTGAGCTCCTGAGTGT CACGGAGATTCACGAGCAGATGTACCTCACAGGATTGACCAGTTCCTTGACCTCTGGACTCTGGATTGGA CTCAACAGTTTAAGTTTAAGCACTGGCTGGCAGTGGGCTGGGGGAAGCCCATTCCGGTATCTGAACTGGC TACCAGGAAGTCCATCATCAGAACCTGGAAAGAGCTGTGTGTCACTAAACCCTGGAAAAAATGCCAAGTG GGAGAATCTGGAATGTGTTCAGAAACTTGGCTACATTTGTAAAAAAGGCAACAATACTTTGAATCCATTT ATCATTCCCTCGGAAAGTGATGTGCCTACTGCCTGCCCTAATCAGTGGTGGCCCTATGCAGGCCACTGCT ACAGGATCTACAGGGAAGAGAAGAAGATCCAGAAATACGCTTTGCAAGCCTGTAGGAAGGAGGGTGGTGA CCTGGCAAGTATCCACAGCATTGAGGAGTTTGACTTCATCTTCTCCCAGCTAGGATACGAGCCAAATGAT GAGCTGTGGATTGGTTTAAATGACATCAAGATTCAGATGTACTTTGAGTGGAGCGATGGGACCCCGGTGA CATTTACTAAATGGCTTCCCGGAGAGCCGAGCCATGAGAACAACAGGCAGGAGGACTGCGTGGTGATGAA AGGGAAGGATGGATACTGGGCAGATAGAGCTTGTGAGCAACCACTGGGTTACATCTGTAAGATGGTCTCA CAAATCCACACTGTAATACCAGAGGGTGCAGAAAAAGGCTGCCGGAAAGGCTGGAAACGGCATGGGTTTT ACTGCTACTTGATTGGATCCACCCTTTCAACGTTCGCTGATGCAAACCAAACATGCACAAATGAGAAGGC TTATTTGACAACAGTTGAAGACAGATATGAACAAGCATTCCTGACTAGTTTGGTTGGATTGAGGCCTGAA AAATATTTTTGGACAGGACTCTCAGATGTTCAAAACAAAGGGACGTTTCGGTGGACTGTGGACGAGCAGG TGCAGTTTACACACTGGAACGCTGACATGCCAGGACGAAAGGCAGGATGCGTTGCTATGAAAACTGGAGT GGCAGGTGGTTTATGGGATGTTTTGAGTTGTGAAGAAAAGGCAAAATTTGTGTGCAAACACTGGGCAGAA GGAGTAACTCGTCCACCAGAGCCCACAACAACTCCTGAACCCAAATGTCCAGAAGACTGGGGTACCACCA GTAAAACCAGCATGTGTTTCAAGCTGTATGCAAAAGGAAAACATGAAAAGAAAACGTGGTTTGAGTCTCG AGATTTTTGCAAAGCTATTGGGGGAGAGCTGGCGAGCATCAAGAGTAAAGATGAACAGCAAGTGATCTGG AGGCTGATTACGAGCAGTGGAAGCTACCATGAGCTGTTTTGGCTGGGACTGACCTATGGAAGTCCTTCAG AGGGGTTCACCTGGAGTGATGGTTCTCCTGTTTCCTATGAAAATTGGGCTTATGGAGAGCCAAATAATTA CCAAAACGTTGAATATTGTGGTGAGTTGAAAGGTGACCCCGGCATGTCCTGGAATGATATTAACTGTGAA CACCTCAACAACTGGATCTGTCAGATCCAAAAAGGGAAAACACTACTACCCGAGCCTACACCCGCTCCTC AAGACAATCCACCAGTGACTGCAGATGGGTGGGTTATTTACAAAGACTACCAGTACTATTTCAGCAAGGA GAAGGAAACCATGGACAACGCGCGGGCCTTCTGCAAGAAGAATTTTGGTGATCTTGCTACCATTAAAAGC GAAAGTGAAAAGAAGTTTCTGTGGAAATATATAAACAAGAATGGTGGGCAGTCACCGTATTTTATTGGCC TGTTGATCAGCTTGGATAAGAAGTTCATTTGGATGGACGGAAGCAAAGTCGATTTTGTAGCTTGGGCTAC GGGGGAACCCAACTTTGCAAATGATGATGAAAACTGTGTAACAATGTACACAAATTCAGGGTTCTGGAAT GATATCAACTGCGGTTATCCAAATAACTTCATCTGCCAGCGACATAATAGCAGCATCAATGCCACTGCCA TGCCTACCACACCCCCAACTCCAGGTGGCTGCAAGGAAGGTTGGCATTTGTACAACAACAAGTGCTTTAA AATTTTTGGGTTTGCTGAAGAAGAGAAAAAGACCTGGAAAGAGGCACGGAAAGCTTGCATAGGATTGAAA GGAAACCTGGTGTCCATAGAGAATGCAAAAGAGCAAGCATTTGTCACTTATCACATGAGAGACTCCACTT TCAACGCCTGGACTGGACTGAATGACGTCAACTCAGAGCACACGTTCCTTTGGACAGACGGACGAGGAGT TCATTATACAAACTGGGGGAAAGGCTATCCCGGCGGAAGGAGAAGCAGTCTGTCGTACGAAGATGTTGAC TGTGTTGTTGTGATTGGTGGCAATTCACGAGAGGCAGGGACCTGGATGGATAGCACCTGTGACAGTAAAC AAGGCTATATATGCCAAACACAGACCGACCCTTCTGTGCCTATCTCTCCAACCACGGCACCAAAAGATGG CTTTGTTAAGTATGGTAAAAGCAGCTATGCCCTTATGAAATTGAAGTCGCCATGGCACGAAGCAGAGAAA TACTGCAAGGATCGTACTTCCCTGCTTGCCAGCATTCTTGACCCCTATAGTAATGCATTTGCATGGATGA AAATGCACCCATTTAATGTACCCATATGGATTGCCCTGAACAGCAACTTGACCAACAATGAGTACACTTG GACAGATAAATGGAGGGTGCGGTACACTAACTGGGGAACTGATGAACCCAAGCTTAAGTCAGCATGCGTT TACATGGATGTCGATGGCTACTGGAAGACATCATACTGCAATGAAAGCTTTTACTTTCTCTGCAAAAAAT CAGACGAAATCCCGGCTACGGAACCCCCACAGCTGCCTGGCAAATGTCCAGAGTCAGAGCAAACTGCGTG GATCCCTTTCCACGGCCACTGTTACTACATTGAATCTTCTTTTACAAGGAGTTGGGGTCAAGCCTCCCTG GAGTGCCTTCGAATGGGTGCCTCCCTGGTTTCCATCGAGACTGCTGCCGAATCCAGTTTTCTGTCATACC GTGTTGAACCTCTTAAAAGTAAAACCAATTTTTGGATAGGCATGTTCCGAAATGTTGAAGGAAAGTGGCT TTGGTTGAATGACAATCCTGTGTCCTTTGTCAACTGGAAAACGGGTGACCCTTCGGGTGAACGGAATGAT TGTGTAGTTCTATCTTCATCTTCTGGCCTTTGGAATAATATCCACTGTACTTCATACAAAGGATTTATTT GTAAAATGCCAAAAATTATTGATCCTGTAACTACACATTCATCTATTACAACCAAAGCTGACCAAAGGAA GATGGATCCTCAACCCAAGGGCTCTTCCAAAGCAGCGGGAGTGGTCATCGTGGTCCTTCTGATTGTGATA GGTGCCGGTGTTGCGGCCTATTTCTTTTACAAGAAAAGGCGTGTACTGCACATACCTCAAGAGGCCACCT TTGAAAACACTCTCTACTTCAACAGTAATCCAAGTCCTGGAACAAGTGACACGAAAGATCTCGTGGGCAA CATCGAACAGAATGAGCATGCGGTCATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR203299 representing NM_001106123 Red=Cloning site Green=Tags(s) MRLPLLLAFISVIPVAVQLLDSRQFLIYNEDHKRCVDALSAISVQTATCNPEAESQKFRWVSESQIMSVA FKLCLGVPSKTDWASVTLYACDSKSEFQKWECKNDTLFGIKGTELYFNYGNRQEKNIKLYKGSGLWSRWK VYGTTDDLCSRGYEAMYSLLGNANGAVCAFPFKFENKWYADCTSAGRSDGWLWCGTTTDYDTDKLFGFCP LQFEGSKRLWNTDPLTGILYQINSKSALTWHQARASCKQQNAELLSVTEIHEQMYLTGLTSSLTSGLWIG LNSLSLSTGWQWAGGSPFRYLNWLPGSPSSEPGKSCVSLNPGKNAKWENLECVQKLGYICKKGNNTLNPF IIPSESDVPTACPNQWWPYAGHCYRIYREEKKIQKYALQACRKEGGDLASIHSIEEFDFIFSQLGYEPND ELWIGLNDIKIQMYFEWSDGTPVTFTKWLPGEPSHENNRQEDCVVMKGKDGYWADRACEQPLGYICKMVS QIHTVIPEGAEKGCRKGWKRHGFYCYLIGSTLSTFADANQTCTNEKAYLTTVEDRYEQAFLTSLVGLRPE KYFWTGLSDVQNKGTFRWTVDEQVQFTHWNADMPGRKAGCVAMKTGVAGGLWDVLSCEEKAKFVCKHWAE GVTRPPEPTTTPEPKCPEDWGTTSKTSMCFKLYAKGKHEKKTWFESRDFCKAIGGELASIKSKDEQQVIW RLITSSGSYHELFWLGLTYGSPSEGFTWSDGSPVSYENWAYGEPNNYQNVEYCGELKGDPGMSWNDINCE HLNNWICQIQKGKTLLPEPTPAPQDNPPVTADGWVIYKDYQYYFSKEKETMDNARAFCKKNFGDLATIKS ESEKKFLWKYINKNGGQSPYFIGLLISLDKKFIWMDGSKVDFVAWATGEPNFANDDENCVTMYTNSGFWN DINCGYPNNFICQRHNSSINATAMPTTPPTPGGCKEGWHLYNNKCFKIFGFAEEEKKTWKEARKACIGLK GNLVSIENAKEQAFVTYHMRDSTFNAWTGLNDVNSEHTFLWTDGRGVHYTNWGKGYPGGRRSSLSYEDVD CVVVIGGNSREAGTWMDSTCDSKQGYICQTQTDPSVPISPTTAPKDGFVKYGKSSYALMKLKSPWHEAEK YCKDRTSLLASILDPYSNAFAWMKMHPFNVPIWIALNSNLTNNEYTWTDKWRVRYTNWGTDEPKLKSACV YMDVDGYWKTSYCNESFYFLCKKSDEIPATEPPQLPGKCPESEQTAWIPFHGHCYYIESSFTRSWGQASL ECLRMGASLVSIETAAESSFLSYRVEPLKSKTNFWIGMFRNVEGKWLWLNDNPVSFVNWKTGDPSGERND CVVLSSSSGLWNNIHCTSYKGFICKMPKIIDPVTTHSSITTKADQRKMDPQPKGSSKAAGVVIVVLLIVI GAGVAAYFFYKKRRVLHIPQEATFENTLYFNSNPSPGTSDTKDLVGNIEQNEHAVI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001106123
ORF Size	4368 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001106123.2, NP_001099593.2
RefSeq Size	5233 bp
RefSeq ORF	4371 bp
Locus ID	291327
MW	165 kDa

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