Tcof1 (NM_001106143) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR201779 representing NM_001106143 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGGCCAGGAAGCGGCGGGAGCTGCTTCCCCTTATTTACCATCATCTGTTGCAAGCAGGCTACG TCCGCGCGGCGCGGGAAGTTAAGGAGCAGAGCGGCCAGAAGAATTTCCTGACTCATCCCGTCACCCTTCT GGACATCTATACACACTGGCAACAGACCTCAGAGCTCGGCCTGAAGCAGAAGGCAGAGAATGATGAGACA CTGCAAGCCAAGAAGTCTCGAGTGTCAGACCCTATTAGCAGCTCAGAGAGCTCAGAGGAGGAGGAAGAGG AGGCAGAAACGGGAACTGCCAAAGCCACCCCAAGACCGACACCTGTCAATTCCACAGCTGCAGCTTTGCC ATCAAACATGAAACAAAAGGCAAAGACCAAGACAGCCAACAAGACGGTGAACTCCGTGCTGCCCCCTGCG TCCGGAAAGACAGCGGTCCAGCTGCTGTCTGGGAAGTCACCCAAGAAGTCAGCAGAGCCCTTGGCCAGCA TTGTCTTGGCCTCAGAAACTGAGGAGGAGGGCAGTGCCCAAGTCCTGGGACCCACTGCCAAGCCTGGAAT GGTGTCAGCAGGCCAAGCCAGCAGTTCCAGCGAGGATACCTCCAGCTCAAGCGATGAGACAGATGTGGAG GTGAAACCCTCAACAAAACCACAGGCCAAAGCTCCAGTAACTCCTGCCAAGGATCCTCCAGCAAGAACAG CCTCAGGCCCTACCAAGCTAGGGGATGTGACACCCGCATCAGCAAGGGCTGCAGCCACTAAAGCGGCAGA GTCTGAGAGCAGTGAGGAGGACTCAGAGAGTGAGGACGAGGCCCCTGCTGGCCTGCCTAGCCAGGTAACA ACCCCTGGAAAAGGTCTTCATGTCAGAGCTGCCTCAGTTTCTGCCAAGGGGATGTCTAGGAAAGGGCCCA TCTCAGCAACCCCAGAGAAGACTGGGCCCATAACCCCCCAAGCCAAGACAGGAAGGCCACAGAAGGATAT GGAAACCAGCAGTGAGGACGATTCTGACAGTGAGGATGAGATGCCAGTCACTGTGACCACTCCTCAGGCA AGGCCTTCCGGGAAGAGCCCTCAGGTCAGAGGTACCTCTGCCCCTGCCAAGGAGTCATCCCAGAAAGGGG CTCCTGCAGTCACCCCTGGGAAGACAAGACCTGTGGCAGCCCAGGCCCAGGCAGGGAAGCCAGAGACCAG GAGCAGTGAGGAGTCAGAGAGCGACAGTGGAGAGACACCAGCAGCTGGGACTCTAACTACAAGTCCTGCC AAGGTGAAACCTTTGGGGAAAAGCCCCCAGGTCAGACCTGCCTCCACTGCAAACCTGGGGTCATCTAGAA AAGGAGCCAACCCACCCTGCCTTGGGAAGGTGGGGTCAGCAGTTCTCAAGGTCCAAACGGGAAAGGAAGA AGAGGACTCAGAGAGCAGCAGCGAGGAGTCGGACAGTGACGGGGCTGTGAACGCAGCCAAGGCAAAGCCC CCAGGGAGAAAAGCCACTCCAGCACTCCCTCGGAAGACGGGGCCTGTGGCCACCCAGGTCAAGACTGACA AGGGCAAAGACCACGCAGAGAGCAGCGAGGAGTCGACCGACAGCGAGGAGGAGGCAGCACCAGCAGCTTC TGCTGCTCAGGCTAAACCAGCTCCGATAAAGCAGATGAAAGCTTCCCCTAGGAAAGGCACGGCTGCATCC ACCACAGGGGCGAGCGCCTCGTCCCCTCGTAAGGCAGGAACAAAGACCTCTTCAGCCAGCCTATCATCCC TGGCTCTGCCCAAGGGCACCCAGAAGCCAGATGTGGACTCCTCTAGCGAGTGGGAGTCAGAAGGAGCTGC TCCTGGCACCACCGGGGTACAGGGAAAGTCTGGGGGAAAGGGCCTCCAAGGGAGAGCTGCCTCAGGACAA GGGGTGGCCCCACTGCACGCTCAGAAGACAGGGCCTTCAGGTGCCCAGGTCAAAGCCACAGCACAGGAAG ACTCAGAGAGCAGCGAAGAGGAATCCAGCAGTGAAGAAGAGGATGAGACCCCAGCACAGGTCATGGCCTT GGGGAGACTTCCTCCGGCCAAAGCCAACCCACCTCCCACTAAGACACCTCTGGCATCTGCATCTGGAAAA GCGGCCGCAGTAGTTCCTCCACCCAAGGGAAAAGCACCTGCGAGTACTGTTCAGAACAGCACCATCTCTG CCAGGGGCCAGCGGGCTGTGCCGGCCACGGGAAAAGCAGGGGCCCCAGCAACCCAAGCACAGAAGGGGCC CATGGCTGGCACAGGGGAGGACTCAGAGAGCAGCAGTGAAGAGGAGTCTGACAGTGAAGAAGAAACTCCA GCCCAGGTCAAGCCTGTGGGGAAGACCTCTCAGGTCAGAGCTGCCTCGGCCCCTGTCAAGGAGTCTCCTA ACAAAGGAGCTTATTCAGGAACCTCCAGGAAGACAGGGCCTCCAGCTACCCAAGCTCAGACAGGGAAGAC AGAGGACTCAGAGAGCAGTAGTGAGGAATCTGACAGTGACAGAGAGATACCACCAGCCATCACCCCAGCC CAGGATGGTATCTCCCAGTCTACCAGAAGCAAACTCTCAGGTCTGGCACCCCCAGAGAAGAGCACAGAAG AGTCCTCAGAGAGCAGTGATGAGGATCTGCCATCTGGCCAGGCCATTAAATCCCCTCCAATTTCTGTCAA CCCTAATCGTGGTCCAGCTGCCCCAGTTCCAACCCCAGAACAACACCAGGCTGTGAACACAAGGAAGGCC CAGGCCTCAGGCAGCACTGCCCAGAGCTCCTCCTCCGAGAGTGAGGATGAGGACATGATTCCTGCCACAC AACCCCCCACCCTTGCCATCAGAACCAATGTGACTACGCCCACAGCCCTCTCACAAACAGCCGCCCAACC CAGCAAGAGTGAGCAGTCTAGTCGGATGCCAAAAGGCAAGAAACCAAAGACAGCGTCCACTCAGATCAGC AGTGCCATGGAAGCACTCCCCGTGACGCTTCCCCAGAGCACACCTGCCCAGTCCAAAACCACCAACAAGC TGGGGGACCCCAAACTTGCTGAGAAGCAGCAGCTTACCCCAGGCTACCCCAAAGCCCCCAGGAGCTCAGA GGACAGCAGTGACACCTCTTCAGAGAGCGAGGAGGATGCCAAGAGACCCCAGATGTCCAAGTCGTCCCAA AGGCTGGATCCAGACGCTTCCCAGAAGGAAACTGTGGTAGAGGAGACCCCTACAGAATCCAGCGACGACG AGATGGTGGCCCCCTCACAGTCTCTCCTCTCAGGTTATGTGACTCCCGGCTTAACTGTGGCCAATTCCCA GCCTTCAAAAGCTACTCCTAGGCCAGACGCCAACCCCTTGGTTTCCTCTGCTCCAGCCACCAAAGATAAC CCAGATGGCAAGCAGAAATCAAAATCCCAAGACTCCACTGCAGACACCACACTCCGTAAAACCGGTAGGA AAGAGGCCTCCTCAGGATCCACACCTCAGAAGCCCAAGAAGCCCAAGAAGAGCACCTTGAGCTCACCAGC CCCGGCACAGACACTGCCGAACAGCATCACCCAGCGCCTCCTGGAGCAGCCCTGGCCCCTGAGTGAGGCA CAGGTGCAGGCCTCTGTGATGAAGGTCCTGACAGAACTGCTGGAGCAGGAAAGGCAGAAGGCCACCGAGG CCATCAAGGAGAGTGGAAAGAAGGGCCAGAAACGGAAACTATCAGGGGACCAGGTAGAAGCTGGGGCCCC AAAGAACAAGAAGAAGAAGCAGCAGCTTGCGGCTGGGACAAGTGCTGGTTCCCCAGAAAAGGCCTCCAGG ACTTCCAAGGCAAAATCAAAACTAAACAAAGGGAGTGCTGGTGGCAAGGGGAAGGGGTCTCCTGTCCCCC AAGGAGCCAAGGAGAAGCCCGAAGGCAAGCTGGGGATAAAGCTTGAGAGTGGAGAGCAGAGTGACCCGAA GAGCAAGAAGGAAAAGAAGAAATCCAGTAAGAAAAGAAAAAAGGAAAAAAGACCCCAGCCAAGGACTCTG CCTCACCGGTCCAGAAGAAGAAAAAGAAGAAGAAGAAGACAGCCGAGCCTGCTGTGTGAGGGCCAGGCAG CGCATAGAACAGAGTCTTGGCAGAGCGTGACCATCCCCAGGCCTCTGACCTCTGGGCACTGTGAGTTGGG ACTGGACTTTTTAATTTGCCCTCCCTCGGCAGAAGGTGATGGCTGGCGTTGCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR201779 representing NM_001106143 Red=Cloning site Green=Tags(s) MAEARKRRELLPLIYHHLLQAGYVRAAREVKEQSGQKNFLTHPVTLLDIYTHWQQTSELGLKQKAENDET LQAKKSRVSDPISSSESSEEEEEEAETGTAKATPRPTPVNSTAAALPSNMKQKAKTKTANKTVNSVLPPA SGKTAVQLLSGKSPKKSAEPLASIVLASETEEEGSAQVLGPTAKPGMVSAGQASSSSEDTSSSSDETDVE VKPSTKPQAKAPVTPAKDPPARTASGPTKLGDVTPASARAAATKAAESESSEEDSESEDEAPAGLPSQVT TPGKGLHVRAASVSAKGMSRKGPISATPEKTGPITPQAKTGRPQKDMETSSEDDSDSEDEMPVTVTTPQA RPSGKSPQVRGTSAPAKESSQKGAPAVTPGKTRPVAAQAQAGKPETRSSEESESDSGETPAAGTLTTSPA KVKPLGKSPQVRPASTANLGSSRKGANPPCLGKVGSAVLKVQTGKEEEDSESSSEESDSDGAVNAAKAKP PGRKATPALPRKTGPVATQVKTDKGKDHAESSEESTDSEEEAAPAASAAQAKPAPIKQMKASPRKGTAAS TTGASASSPRKAGTKTSSASLSSLALPKGTQKPDVDSSSEWESEGAAPGTTGVQGKSGGKGLQGRAASGQ GVAPLHAQKTGPSGAQVKATAQEDSESSEEESSSEEEDETPAQVMALGRLPPAKANPPPTKTPLASASGK AAAVVPPPKGKAPASTVQNSTISARGQRAVPATGKAGAPATQAQKGPMAGTGEDSESSSEEESDSEEETP AQVKPVGKTSQVRAASAPVKESPNKGAYSGTSRKTGPPATQAQTGKTEDSESSSEESDSDREIPPAITPA QDGISQSTRSKLSGLAPPEKSTEESSESSDEDLPSGQAIKSPPISVNPNRGPAAPVPTPEQHQAVNTRKA QASGSTAQSSSSESEDEDMIPATQPPTLAIRTNVTTPTALSQTAAQPSKSEQSSRMPKGKKPKTASTQIS SAMEALPVTLPQSTPAQSKTTNKLGDPKLAEKQQLTPGYPKAPRSSEDSSDTSSESEEDAKRPQMSKSSQ RLDPDASQKETVVEETPTESSDDEMVAPSQSLLSGYVTPGLTVANSQPSKATPRPDANPLVSSAPATKDN PDGKQKSKSQDSTADTTLRKTGRKEASSGSTPQKPKKPKKSTLSSPAPAQTLPNSITQRLLEQPWPLSEA QVQASVMKVLTELLEQERQKATEAIKESGKKGQKRKLSGDQVEAGAPKNKKKKQQLAAGTSAGSPEKASR TSKAKSKLNKGSAGGKGKGSPVPQGAKEKPEGKLGIKLESGEQSDPKSKKEKKKSSKKRKKEKRPQPRTL PHRSRRRKRRRRRQPSLLCEGQAAHRTESWQSVTIPRPLTSGHCELGLDFLICPPSAEGDGWRCC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001106143
ORF Size	4185 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001106143.1, NP_001099613.1
RefSeq Size	4627 bp
RefSeq ORF	4188 bp
Locus ID	291571
MW	144.7 kDa

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