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Thoc5 (NM_001012153) Rat Tagged ORF Clone
CAT＃: RR201699
- TrueORF®
Thoc5 (Myc-DDK-tagged ORF) - Rat THO complex 5 (Thoc5), (10 ug)
"NM_001012153" in other vectors (3)
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CNY 8,740.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Fmip |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR201699 representing NM_001012153
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATCAGAATCCAGCAAAAAAAGGAAACCCAAAGTGATCCGAAGTGATGGAACCCCAACTGAAGGGA AACGGAATCGATCTGACACAGAGCAGGAAGGCAGATACTATAGCGAGGAGGCTGAGGTTGACCTGCGGGA CCCCGGCAGAGACTATGAGTTGTACAAGTACACTTGCCAGGAGCTACAGAGGTTGATGGCTGAGATCCAG GACCTGAAGAGCAAGGGCAGCAAGGACGTGGCCATTGAAATAGAAGAACGAAGGATCCAGAGCTGTGTGC ATTTCATGACTCTAAAGAAGCTTAACCGATTAGCCCATATCAGGTTAAAGAAAGGAAGAGATCAGACCCA CGAGGCCAAGCAGAAAGTCGATGCCTACCACCTACAGCTGCAGAACCTGCTCTACGAAGTGATGCACCTG CAGAAGGAGATCACCAAGTGTCTGGAGTTCAAGTCAAAGCATGAAGAAATCGATCTGGTCAGTTTAGAGG AATTTTATTCAGAAGCTCCACCAAACATCAGCAAGGCCGAGATCACCATGGGAGACCCTCACCAACAGAC GCTGGCACGTTTGGACTGGGAGCTGGAGCAGCGGAAAAGGCTGGCAGAGAAGTACCGAGAGTGTCTGTCC AACAAGGAGAAGATCCTGAAGGAGATCGAGGTGAAGAAGGAGTATCTCAGCAGCCTCCAGCCCCGCCTCA ACAGCATCATGCAGGCTTCCCTGCCGGTGCAAGAGTACCTGTTCATGCCATTCGACCAGGCTCACAAGCA GTACGAGACCGCCAGGCACCTGCCGCCTCCCCTCTACGTGCTCTTTGTGCAGGCCACCGCATACGGGCAG GCCTGTGATAAGACGTTGTCTGTGGCGATTGAAGGCAGTGTGGATGAAGCCAAAGCCCTTTTCAAGCCCC CCGAGGACTCCCAAGATGATGAGAGTGACTCGGATGCTGAAGAGGAGCAGACCACGAGGCGTCGGCCCAC ACTCGGGGTTCAGTTGGATGATAAGCGCAAGGAGATGCTGAAAAGGCACCCGCTGTCTGTCCTGCTGGAT CTGAAGTGCAAAGACAACAGTGTGCTCCATCTGACTTTCTTCTATCTCATGAACCTCAACATCATGACAG TGAAAGCCAAAGTGACGACCGCCATGGAGCTGATCACCCCCATCAGTGCAGGCGACTTACTGTCTCCTGA CTCGGTCTTGAGCTGCTTGTATCCTGGAGATCATGGAAAGAAAACTCCAAATCCAGCCAATCAGTATCAG TTTGATAAAGTTGGCATCCTGACTTTGAGAGACTATGTACTTGAGTTAGGTCACCCCTACTTGTGGGTAC AGAAGCTGGGTGGCCTCCACTTCCCCAAAGAGCAGCCCCAGCACACGGTGATGGCTGACCACTCACAGAG CGCTAGCCACATGGAGACCACCATGAAACTGCTCAAGACTAGGGTACAGTCCCGCCTGGCTCTCCACAAA CAGTTCGCATCTCTGGAACATGGCATTGTGCCAGTCACCAGCGAGTGCCAGTACCTCTTCCCTGCCAAGG TTGTTTCCCGACTGGTAAAGTGGATGATAATGGCCCATGAAGATTACATGGAGCTGCACTTCACTAAGGA CATTGTAGAGGCCGGCCTGGCTGGGGACACCAACCTCTACTACCTGGCGCTTATCGAAAGGGGAACAGCT AAGCTTCAGGCTGCTGTGGTACTGAACCCTGGCTACTCCTCCATCCCACCTGTTTTCCGACTCTGTCTAA ACTGGAAAGGGGAGAAAACCAACAGCAACGATGACAACATTCGGGCTATGGAGAGTGAAGTCAATGTGTG CTATCGGGAGCTCTGCGGCCCTCGGCCCAGTCACCAGCTCTTGACCAACCAGCTGCAGAGGCTGTGTGTG CTGCTGGATGTCTACCTGGAGACCGAGAGCCATGATGACAGTGTAGAGGGCCCCAAGGAATTTCCCCAGG AGAAGATGTGCCTGCGGCTCTTTAGGGGTCCAAGTAGGATGAAGCCGTTTAAATATAACCACCCTCAGGG ATTCTTCAGCCACCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR201699 representing NM_001012153
Red=Cloning site Green=Tags(s) MSSESSKKRKPKVIRSDGTPTEGKRNRSDTEQEGRYYSEEAEVDLRDPGRDYELYKYTCQELQRLMAEIQ DLKSKGSKDVAIEIEERRIQSCVHFMTLKKLNRLAHIRLKKGRDQTHEAKQKVDAYHLQLQNLLYEVMHL QKEITKCLEFKSKHEEIDLVSLEEFYSEAPPNISKAEITMGDPHQQTLARLDWELEQRKRLAEKYRECLS NKEKILKEIEVKKEYLSSLQPRLNSIMQASLPVQEYLFMPFDQAHKQYETARHLPPPLYVLFVQATAYGQ ACDKTLSVAIEGSVDEAKALFKPPEDSQDDESDSDAEEEQTTRRRPTLGVQLDDKRKEMLKRHPLSVLLD LKCKDNSVLHLTFFYLMNLNIMTVKAKVTTAMELITPISAGDLLSPDSVLSCLYPGDHGKKTPNPANQYQ FDKVGILTLRDYVLELGHPYLWVQKLGGLHFPKEQPQHTVMADHSQSASHMETTMKLLKTRVQSRLALHK QFASLEHGIVPVTSECQYLFPAKVVSRLVKWMIMAHEDYMELHFTKDIVEAGLAGDTNLYYLALIERGTA KLQAAVVLNPGYSSIPPVFRLCLNWKGEKTNSNDDNIRAMESEVNVCYRELCGPRPSHQLLTNQLQRLCV LLDVYLETESHDDSVEGPKEFPQEKMCLRLFRGPSRMKPFKYNHPQGFFSHR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001012153 |
| ORF Size | 2046 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001012153.1, NP_001012153.1 |
| RefSeq Size | 2227 bp |
| RefSeq ORF | 2049 bp |
| Locus ID | 360972 |
| UniProt ID | Q68FX7 |
| MW | 78.7 kDa |
| Gene Summary | Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in transcription elongation and genome stability. Involved in alternative polyadenylation site choice by recruiting CPSF6 to 5' region of target genes; probably mediates association of the TREX and CFIm complexes (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
THOC2 and THOC5 Regulate Stemness and Radioresistance in Triple‐Negative Breast Cancer
,null,
Advanced Science
,PubMed ID 34708581
[Thoc5]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN201699 | Thoc5 (untagged ORF) - Rat THO complex 5 (Thoc5), (10 ug) |
CNY 8,740.00 |
|
| RR201699L3 | Lenti ORF clone of Thoc5 (Myc-DDK-tagged ORF) - Rat THO complex 5 (Thoc5), (10 ug) |
CNY 10,550.00 |
|
| RR201699L4 | Lenti ORF clone of Thoc5 (mGFP-tagged ORF) - Rat THO complex 5 (Thoc5), (10 ug) |
CNY 11,690.00 |
