Syngap1 (NM_181092) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Syngap
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200179 representing NM_181092 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGGTCTCGAGCCTCCATCCATCGGGGGAGCATCCCCGCGATGTCCTATGCCCCCTTCAGAGATG TACGGGGACCCCCTATGCACCGAACCCAATACGTTCATTCCCCGTATGACCGTCCCGGTTGGAACCCCCG GTTCTGCATCATCTCTGGGAACCAGCTGCTCATGCTGGATGAGGATGAGATACACCCCCTTCTGATCCGC GACCGGAGGAGCGAGTCCAGCCGAAACAAACTGCTGAGACGCACCGTCTCTGTGCCAGTGGAGGGGCGGC CCCACGGCGAGCATGAATACCACTTGGGTCGCTCGAGGAGGAAGAGTGTCCCCGGGGGGAAACAGTACAG CATGGAAGCCGCCCCCGCTGCGCCCTTCCGGCCCTCGCAAGGCTTCCTGAGCCGGAGGCTAAAAAGCTCC ATCAAACGTACAAAGTCACAACCCAAACTTGACCGGACCAGCAGCTTTCGACAGATCCTGCCTCGCTTCC GAAGTGCTGACCATGACCGGGCCCGGCTGATGCAGAGCTTCAAGGAGTCTCACTCCCATGAGTCCCTGCT GAGTCCCAGCAGTGCTGCTGAGGCCCTGGAGCTCAACCTGGATGAAGACTCCATTATCAAGCCAGTACAC AGCTCCATCCTGGGCCAGGAGTTCTGCTTTGAGGTAACAACATCGTCTGGGACAAAATGTTTTGCCTGTC GGTCTGCAGCCGAAAGGGACAAATGGATTGAGAATCTACAGAGGGCTGTGAAACCCAACAAGGACAACAG CCGCCGGGTAGATAACGTGCTGAAACTATGGATCATAGAAGCTCGAGAGCTGCCCCCCAAGAAGCGATAT TACTGCGAGTTATGCCTGGACGACATGCTCTATGCACGGACCACTTCCAAGCCCCGCTCAGCCTCAGGAG ACACTGTCTTTTGGGGCGAGCACTTCGAGTTTAACAACCTGCCTGCTGTCCGGGCGCTGCGGCTGCATCT GTACCGTGACTCGGACAAAAAGCGGAAGAAGGACAAGGCAGGCTACGTTGGCCTGGTGACTGTTCCAGTG GCCACCCTGGCTGGGCGCCACTTCACAGAGCAGTGGTACCCCGTGACCCTGCCAACAGGAAGTGGGGGCT CTGGGGGTATGGGCTCGGGGGGAGGAGGGGGGTCAGGGGGCGGCTCAGGGGGCAAAGGGAAAGGAGGCTG TCCTGCTGTGCGGCTGAAGGCCCGTTACCAGACAATGAGTATCCTGCCCATGGAGCTATATAAGGAGTTT GCAGAATATGTGACCAACCACTACCGCATGCTGTGTGCCGTGCTGGAGCCCGCCCTCAATGTCAAGGGCA AGGAGGAGGTCGCTAGTGCACTGGTTCACATCCTGCAAAGCACAGGCAAGGCCAAGGACTTCCTTTCAGA CATGGCCATGTCAGAGGTAGACCGGTTCATGGAGCGGGAACACCTCATATTCCGCGAGAACACGCTCGCC ACTAAAGCCATAGAAGAGTATATGAGACTGATTGGCCAGAAATACCTCAAGGATGCCATTGGGGAGTTCA TCCGGGCTCTGTATGAATCTGAGGAGAACTGTGAAGTAGACCCCATCAAGTGCACAGCGTCCAGTCTGGC AGAGCACCAGGCCAACCTGCGGATGTGCTGTGAGTTGGCCCTGTGCAAGGTGGTCAACTCCCATTGCGTG TTCCCGGGGGAGCTGAAGGAGGTGTTTGCATCATGGCGGCTGCGCTGTGCAGAGCGGGGCCGGGAGGACA TTGCTGACAGGCTGATCAGCGCCTCGCTCTTCCTGCGCTTCCTCTGCCCGGCCATCATGTCGCCCAGTCT GTTTGGACTGATGCAGGAGTACCCAGATGAGCAGACCTCACGAACCCTCACCCTCATCGCCAAGGTTATC CAGAACCTGGCCAACTTTTCCAAGTTTACCTCAAAGGAGGACTTCCTGGGCTTCATGAACGAGTTTCTGG AGCTGGAGTGGGGTTCTATGCAGCAATTCTTGTATGAGATATCCAACCTGGACACACTGACCAACAGCAG CAGTTTTGAGGGCTACATAGACTTGGGCCGCGAGCTCTCCACACTTCACGCCCTGCTCTGGGAGGTGCTG CCCCAGCTCAGCAAGGAAGCCCTCCTGAAGCTGGGCCCGCTGCCCCGGCTCCTCAGCGACATCAGCACAG CCCTGAGGAACCCTAACATCCAAAGGCAGCCGAGCCGCCAGAGCGAGCGCGCTCGGTCTCAGCCCATGGT GCTGCGCGGGCCGTCAGCCGAGATGCAGGGCTACATGATGCGGGACCTCAACAGCTCCATCGACCTTCAG TCCTTCATGGCTCGAGGCCTCAACAGCTCTATGGACATGGCTCGCCTCCCCTCCCCAACCAAGGAGAAAC CCCCGCCGCCCCCTCCCGGTGGGGGTAAAGACCTGTTCTATGTGAGCCGGCCACCACTGGCCCGGTCCTC CCCAGCATACTGCACGAGCAGCTCGGACATCACAGAGCCGGAGCAGAAGATGCTGAGTGTCAACAAGAGT GTGTCCATGCTGGACCTGCAGGGCGACGGGCCTGGGGGCCGCCTTAACAGCAGTAGTGTTTCCAACCTGG CAGCTGTTGGGGACCTGTTGCACTCAAGCCAGGCTTCACTGACAGCAGCCTTGGGGTTGCGGCCTGCACC TGCCGGGCGCCTCTCCCAAGGGAGTGGCTCTTCCATCACAGCAGCCGGCATGCGCCTCAGCCAGATGGGT GTCACTACGGATGGTGTCCCCGCCCAGCAACTGCGCATCCCTCTTTCCTTCCAGAACCCTCTCTTCCATA TGGCTGCCGATGGACCAGGGCCCCCAGCAGGCCATGGAGGGAGCAGTGGCCATGGTCCACCTTCCTCCCA TCACCACCACCACCACCATCACCATCACCGAGGGGGAGAACCCCCAGGGGACACTTTTGCCCCGTTCCAT GGCTATAGCAAGAGCGAGGACCTCTCTACAGGGGTCCCTAAGCCCCCTGCGGCCTCCATCCTTCACAGCC ACAGCTACAGTGATGAGTTTGGACCCTCTGGTACTGATTTTACCCGTCGGCAGCTCTCACTTCAGGACAA CCTACAGCACATGCTCTCCCCGCCCCAGATCACCATCGGTCCCCAGAGGCCAGCTCCCTCAGGGCCAGGA GGGGGCAGTGGTGGGGGCAGTGGTGGGGGCGGTGGGGGCCAGCCACCTCCCTTGCAGAGGGGCAAATCTC AGCAGTTGACAGTGAGTGCTGCCCAGAAACCCCGGCCGTCCAGCGGGAACCTATTGCAGTCCCCGGAACC AAGTTATGGTCCTGCCCGTCCACGGCAACAGAGCCTCAGCAAAGAGGGCAGCATTGGGGGCAGCGGGGGC AGCGGTGGCGGAGGGGGTGGGGGGCTCAAGCCCTCCATCACCAAGCAGCATTCCCAGACTCCATCCACGC TGAACCCCACGATGCCGGCCTCGGAGCGGACTGTAGCCTGGGTGTCCAATATGCCTCACCTGTCCGCTGA CATCGAGAGTGCACACATTGAGCGGGAAGAGTACAAGCTGAAGGAGTACTCGAAGTCCATGGACGAGAGC CGACTGGACAGGGTGAAGGAGTACGAGGAGGAGATCCACTCACTGAAGGAAAGGCTACACATGTCCAACC GGAAGCTGGAAGAGTACGAGCGGAGGCTGCTGTCCCAGGAAGAGCAGACCAGCAAGATCCTGATGCAGTA CCAAGCCCGCCTGGAGCAGAGCGAGAAGCGCTTGAGGCAGCAGCAGGTGGAGAAGGACTCCCAGATCAAG AGCATCATTGGCAGGCTGATGCTGGTGGAGGAGGAGCTGCGCCGGGACCACCCCGCCATGGCTGAGCCGC TGCCTGAACCCAAGAAGAGGCTGCTCGACGCTCAGAGAGGCAGCTTCCCCCCTTGGGTCCAACAAACCCG CGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200179 representing NM_181092 Red=Cloning site Green=Tags(s) MSRSRASIHRGSIPAMSYAPFRDVRGPPMHRTQYVHSPYDRPGWNPRFCIISGNQLLMLDEDEIHPLLIR DRRSESSRNKLLRRTVSVPVEGRPHGEHEYHLGRSRRKSVPGGKQYSMEAAPAAPFRPSQGFLSRRLKSS IKRTKSQPKLDRTSSFRQILPRFRSADHDRARLMQSFKESHSHESLLSPSSAAEALELNLDEDSIIKPVH SSILGQEFCFEVTTSSGTKCFACRSAAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELPPKKRY YCELCLDDMLYARTTSKPRSASGDTVFWGEHFEFNNLPAVRALRLHLYRDSDKKRKKDKAGYVGLVTVPV ATLAGRHFTEQWYPVTLPTGSGGSGGMGSGGGGGSGGGSGGKGKGGCPAVRLKARYQTMSILPMELYKEF AEYVTNHYRMLCAVLEPALNVKGKEEVASALVHILQSTGKAKDFLSDMAMSEVDRFMEREHLIFRENTLA TKAIEEYMRLIGQKYLKDAIGEFIRALYESEENCEVDPIKCTASSLAEHQANLRMCCELALCKVVNSHCV FPGELKEVFASWRLRCAERGREDIADRLISASLFLRFLCPAIMSPSLFGLMQEYPDEQTSRTLTLIAKVI QNLANFSKFTSKEDFLGFMNEFLELEWGSMQQFLYEISNLDTLTNSSSFEGYIDLGRELSTLHALLWEVL PQLSKEALLKLGPLPRLLSDISTALRNPNIQRQPSRQSERARSQPMVLRGPSAEMQGYMMRDLNSSIDLQ SFMARGLNSSMDMARLPSPTKEKPPPPPPGGGKDLFYVSRPPLARSSPAYCTSSSDITEPEQKMLSVNKS VSMLDLQGDGPGGRLNSSSVSNLAAVGDLLHSSQASLTAALGLRPAPAGRLSQGSGSSITAAGMRLSQMG VTTDGVPAQQLRIPLSFQNPLFHMAADGPGPPAGHGGSSGHGPPSSHHHHHHHHHHRGGEPPGDTFAPFH GYSKSEDLSTGVPKPPAASILHSHSYSDEFGPSGTDFTRRQLSLQDNLQHMLSPPQITIGPQRPAPSGPG GGSGGGSGGGGGGQPPPLQRGKSQQLTVSAAQKPRPSSGNLLQSPEPSYGPARPRQQSLSKEGSIGGSGG SGGGGGGGLKPSITKQHSQTPSTLNPTMPASERTVAWVSNMPHLSADIESAHIEREEYKLKEYSKSMDES RLDRVKEYEEEIHSLKERLHMSNRKLEEYERRLLSQEEQTSKILMQYQARLEQSEKRLRQQQVEKDSQIK SIIGRLMLVEEELRRDHPAMAEPLPEPKKRLLDAQRGSFPPWVQQTRV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_181092
ORF Size	3924 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_181092.5
RefSeq Size	5868 bp
RefSeq ORF	3927 bp
Locus ID	192117
MW	144.6 kDa
Gene Summary	This gene encodes a Ras GTPase activating protein that is a member of the N-methyl-D-aspartate receptor complex. The N-terminal domain of the protein contains a Ras-GAP domain, a pleckstrin homology domain, and a C2 domain that may be involved in binding of calcium and phospholipids. The C-terminal domain consists of a ten histidine repeat region, serine and tyrosine phosphorylation sites, and a T/SXV motif required for postsynaptic scaffold protein interaction. The encoded protein negatively regulates Ras, Rap and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor trafficking to the postsynaptic membrane to regulate synaptic plasticity and neuronal homeostasis. Homozygous null mutations in mice result in early post-embryonic lethality, while heterozygous mutant mice display a variety of phenotypes that include learning and memory defects, hyperactivity, and audiogenic seizures. [provided by RefSeq, Nov 2016]

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