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Pip5k1c (NM_001009967) Rat Tagged ORF Clone
CAT＃: RR200081
- TrueORF®
Pip5k1c (Myc-DDK-tagged ORF) - Rat phosphatidylinositol-4-phosphate 5-kinase, type I, gamma (Pip5k1c), transcript variant c, (10 ug)
"NM_001009967" in other vectors (3)
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CNY 8,840.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC124518 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200081 representing NM_001009967
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTAGAGGTGCCGGACGAGGCAGAGAGCGCCGAAGCAGGGGCCGTGACAGCGGAGGCGGCCTGGT CTGCGGAGAGTGGGGCGGCGGCAGGTATGACCCAGAAGAAGGCTATCCTTGCAGAGGCCCCGTTGGTGAC AGGGCAGCCAGGCCCTGGCCATGGGAAGAAGCTGGGCCATCGAGGCGTGGATGCGTCTGGAGAGACTACG TATAAGAAGACCACCTCGTCCACCCTGAAGGGTGCCATCCAACTGGGGATTGGGTACACGGTGGGCAACC TCAGCTCTAAGCCAGAACGCGATGTGCTCATGCAGGACTTCTATGTGGTGGAGAGCATCTTCTTTCCCAG CGAAGGGAGCAACCTTACCCCCGCCCACCACTTCCAGGATTTCCGTTTCAAGACCTATGCACCTGTTGCC TTCCGTTACTTCCGGGAGCTCTTTGGTATCCGTCCGGACGATTACTTGTACTCACTCTGCAACGAGCCAC TCATTGAGCTGTCCAACCCGGGTGCCAGTGGCTCCGTCTTCTACGTCACCAGCGATGACGAGTTCATCAT TAAGACTGTCATGCACAAGGAGGCGGAGTTCCTGCAGAAACTGCTGCCTGGCTACTATATGAACCTCAAC CAGAACCCCCGGACCTTGCTGCCCAAGTTCTATGGGCTGTACTGTGTGCAGTCCGGCGGCAAGAACATTC GAGTGGTGGTCATGAACAATGTGCTGCCCCGTGTCGTCAAGATGCACCTCAAGTTCGACCTCAAGGGCTC CACGTACAAGCGCAGGGCCAGCAAGAAGGAGAAGGAGAAGAGCCTGCCCACCTACAAGGACCTGGACTTC ATGCAGGACATGCCAGAGGGGCTGCTACTGGACTCTGACACCTTTGGCGCCCTGGTCAAGACGCTGCAGC GAGACTGCCTGGTGTTGGAGAGCTTCAAGATAATGGACTACAGCCTGCTGCTGGGTGTGCACAACATTGA CCAGCAGGAGCGGGAGCGCCAGGCCGAGGGCGCCCAGAGCAAGGCTGACGAGAAGCGGCCGGTGGCCCAG AAGGCCCTCTACTCCACAGCCATGGAGTCCATTCAGGGCGGAGCTGCCCGTGGGGAGGCCATTGAGACAG ATGACACGATGGGTGGGATCCCAGCCGTGAATGGGCGTGGGGAGCGCTTGCTCCTACACATCGGAATCAT TGACATTCTGCAGTCCTACAGGTTCATCAAGAAGTTAGAACACACCTGGAAGGCCCTCGTCCACGATGGG GATACCGTCTCAGTCCACCGGCCCAGCTTCTATGCCGAGCGCTTCTTCAAGTTCATGAGCAGCACGGTCT TCCGGAAGAGTTCCTCCCTCAAGTCCTCTCCATCTAAGAAAGGGCGTGGCGCCCTGCTCGCAGTCAAGCC CCTGGGGCCCACCGCAGCCTTCTCAGCTAGCCAGATCCCCAGTGAGAGAGAGGATGTGCAGTACGACCTG CGGGGGGCCCGCAGCTACCCCACGCTGGAGGATGAAGGCCGGCCTGACCTCCTGCCCTGCACCCCACCGT CCTTTGAGGAAGCCACCACAGCCTCTATCGCCACCACCCTGTCATCCACCTCCCTCTCCATCCCAGAGCG GTCCCCTTCAGACACATCAGAGCAGCCCCGGTACAGGCGGCGTACGCAGTCTTCAGGCCAGGATGGCCGG CCCCAGGAGGAGCTGCATGCGGAAGACCTGCAGAAGATAACTGTGCAGGTGGAGCCAGTGTGTGGTGTGG GGGTTGTCGTCCCCAAGGAGCAGGGTGCAGGAGTGGAGGTTCCCCCATCTGGGGCATCGGCAGCAGCCAC GGTGGAAGTAGACGCTGCCAGCCAGGCCTCAGAGCCCGCCAGCCAAGCCTCTGATGAGGAAGATGCGCCT TCCACAGACATCTATTTTTTCGCCCACGGGAGATACTGGCTTTTCTCTCCCCGTCGTCGCCGACTGCGGG CCGTGACACCAAGCCACACAGGCGCTCCCACCGACGAGAGGAGCTGGGTGTACTCCCCGCTTCACTATAG CGCACGGCCCGCCTCCGACGGCGAGAGCGACACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200081 representing NM_001009967
Red=Cloning site Green=Tags(s) MELEVPDEAESAEAGAVTAEAAWSAESGAAAGMTQKKAILAEAPLVTGQPGPGHGKKLGHRGVDASGETT YKKTTSSTLKGAIQLGIGYTVGNLSSKPERDVLMQDFYVVESIFFPSEGSNLTPAHHFQDFRFKTYAPVA FRYFRELFGIRPDDYLYSLCNEPLIELSNPGASGSVFYVTSDDEFIIKTVMHKEAEFLQKLLPGYYMNLN QNPRTLLPKFYGLYCVQSGGKNIRVVVMNNVLPRVVKMHLKFDLKGSTYKRRASKKEKEKSLPTYKDLDF MQDMPEGLLLDSDTFGALVKTLQRDCLVLESFKIMDYSLLLGVHNIDQQERERQAEGAQSKADEKRPVAQ KALYSTAMESIQGGAARGEAIETDDTMGGIPAVNGRGERLLLHIGIIDILQSYRFIKKLEHTWKALVHDG DTVSVHRPSFYAERFFKFMSSTVFRKSSSLKSSPSKKGRGALLAVKPLGPTAAFSASQIPSEREDVQYDL RGARSYPTLEDEGRPDLLPCTPPSFEEATTASIATTLSSTSLSIPERSPSDTSEQPRYRRRTQSSGQDGR PQEELHAEDLQKITVQVEPVCGVGVVVPKEQGAGVEVPPSGASAAATVEVDAASQASEPASQASDEEDAP STDIYFFAHGRYWLFSPRRRRLRAVTPSHTGAPTDERSWVYSPLHYSARPASDGESDT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001009967 |
| ORF Size | 2064 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001009967.2, NP_001009967.2 |
| RefSeq Size | 4343 bp |
| RefSeq ORF | 2067 bp |
| Locus ID | 314641 |
| UniProt ID | Q5I6B8 |
| MW | 75.6 kDa |
| Gene Summary | Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as vesicle mediated transport, cell adhesion, cell polarization and cell migration. Together with PIP5K1A is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle attachment by generating the pool of PtdIns(4,5)P2 that induces controlled actin depolymerization to facilitate Fc-gamma-R clustering. Mediates RAC1-dependent reorganization of actin filaments. Required for synaptic vesicle transport. Controls the plasma membrane pool of PtdIns(4,5)P2 implicated in synaptic vesicle endocytosis and exocytosis. Plays a role in endocytosis mediated by clathrin and AP-2 (adaptor protein complex 2). Required for clathrin-coated pits assembly at the synapse. Participates in cell junction assembly. Modulates adherens junctions formation by facilitating CDH1 trafficking. Required for focal adhesion dynamics. Modulates the targeting of talins (TLN1 and TLN2) to the plasma membrane and their efficient assembly into focal adhesions. Regulates the interaction between talins (TLN1 and TLN2) and beta-integrins. Required for uropodium formation and retraction of the cell rear during directed migration. Has a role in growth factor- stimulated directional cell migration and adhesion. Required for talin assembly into nascent adhesions forming at the leading edge toward the direction of the growth factor. Negative regulator of T-cell activation and adhesion. Negatively regulates integrin alpha-L/beta-2 (LFA-1) polarization and adhesion induced by T-cell receptor. Together with PIP5K1A has a role during embryogenesis and together with PIP5K1B may have a role immediately after birth (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200081 | Pip5k1c (untagged ORF) - Rat phosphatidylinositol-4-phosphate 5-kinase, type I, gamma (Pip5k1c), transcript variant c, (10 ug) |
CNY 8,840.00 |
|
| RR200081L3 | Lenti ORF clone of Pip5k1c (Myc-DDK-tagged ORF) - Rat phosphatidylinositol-4-phosphate 5-kinase, type I, gamma (Pip5k1c), transcript variant c, (10 ug) |
CNY 10,640.00 |
|
| RR200081L4 | Lenti ORF clone of Pip5k1c (mGFP-tagged ORF) - Rat phosphatidylinositol-4-phosphate 5-kinase, type I, gamma (Pip5k1c), transcript variant c, (10 ug) |
CNY 11,780.00 |
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