Usp9x (NM_001135923) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1560056
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200045 representing NM_001135923 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGCCACGACTCGTGGCTCTCCAGTTGGAGGGAATGACAACCAGGGCCAAGCTCCTGATGGACAGT CTCAGCCCCCCCTCCAACAGAATCAGACTTCATCGCCTGATTCATCCAATGAAAATTCCCCTGCAACTCC TCCTGATGAGCAAGGCCAAGGTGATGCTCCTCCCCAGATTGAAGATGAGGAACCTGCATTTCCACACACT GACCTGGCAAAGTTAGATGATATGATCAACAGGCCTCGCTGGGTCGTTCCAGTTTTGCCGAAAGGGGAAT TAGAAGTGCTTTTAGAAGCTGCTATTGATCTTAGTAAGAAAGGCCTTGATGTCAAAAGTGAAGCATGTCA GAGATTTTTCCGAGATGGGCTAACAATCTCATTCACAAAAATCCTTACAGATGAAGCAGTGAGTGGCTGG AAGTTTGAAATTCATAGATGTATTATTAACAATACTCATCGCCTGGTGGAGCTGTGTGTGGCTAAGTTAG CCCAAGACTGGTTTCCACTTCTAGAACTTCTTGCCATGGCCTTAAATCCTCATTGCAAATTCCATATCTA CAATGGTACACGTCCCTGCGAGTCAGTGTCCTCAAGTGTTCAGTTGCCTGAAGATGAACTCTTTGCTCGT TCTCCAGACCCTCGATCACCAAAAGGTTGGCTAGTGGATCTCCTCAACAAATTTGGCACTTTAAATGGAT TCCAGATACTGCATGATCGTTTTATTAATGGATCAGCATTAAATGTTCAGATAATTGCAGCCCTTATTAA ACCATTTGGACAGTGCTATGAGTTTCTTACTCTTCACACGGTGAAAAAATACTTTCTTCCAATAATAGAA ATGGTTCCACAGTTTTTAGAAAACTTAACTGATGAAGAGCTGAAGAAAGAAGCAAAGAATGAAGCCAAAA ATGATGCTCTCTCAATGATTATTAAATCATTGAAGAGTTTAGCTTCACGAGTTCCTGGACAGGAAGAAAC TGTAAAGAACTTAGAAATATTTAGGTTAAAAATGATACTTAGATTATTGCAAATTTCTTCCTTCAATGGA AAGATGAATGCACTGAATGAGGTTAATAAGGTGATTTCCAGTGTGTCATACTATACCCATCGGCATGGCA GTTCTGAGGAAGAAGAGTGGCTCACAGCAGAGAGGATGGCTGAATGGATACAGCAGAACAATATTTTATC AATAGTTTTACGAGATAGTCTTCATCAACCACAGTATGTAGAGAAACTAGAAAAGATTCTTCGCTTTGTC ATAAAAGAAAAAGCTCTGACCTTGCAAGATCTTGATAATATCTGGGCAGCACAGGCAGGGAAACATGAAG CCATCGTAAAGAATGTCCATGATCTACTGGCCAAATTGGCATGGGATTTTTCTCCTGAACAACTTGATCA TCTTTTTGATTGCTTTAAGGCCAGTTGGACAAATGCAAGTAAAAAGCAACGTGAAAAGCTCCTTGAGCTG ATTCGTCGTCTTGCAGAAGATGATAAAGATGGTGTGATGGCACATAAAGTGTTGAACCTTCTGTGGAATC TAGCTCACAGTGATGATGTGCCTGTGGATATCATGGACTTGGCTCTCAGTGCTCACATCAAAATACTAGA TTATAGTTGCTCCCAGGACCGGGACACACAAAAGATCCAGTGGATAGATCGCTTCATAGAAGAACTTCGC ACAAATGACAAGTGGGTCATTCCTGCACTGAAACAAATTAGAGAAATTTGCAGTTTGTTTGGTGAAGCAC CTCAAAATTTGAGTCAAACTCAGAGAAGTCCCCATGTATTTTATCGCCATGATTTAATCAATCAACTTCA GCACAATCATGCCCTAGTTACTTTGGTAGCAGAAAACCTTGCAACTTACATGGAAAGCATGAGACTGTAT GGCAGAGACAATGAAGACTATGACCCACAAACTGTGAGAGTGGGAAGTAGATATAGTCATGTTCAAGAAG TCCAAGAACGTCTTAACTTTCTTAGATTTTTATTGAAGGATGGCCAGCTATGGCTGTGTGCTCCTCAGGC AAAACAAATATGGAAGTGCTTAGCAGAGAATGCGGTTTATCTTTGTGACCGTGAAGCCTGCTTTAAGTGG TATTCCAAATTAATGGGAGATGAACCAGACTTAGATCCTGATATCAATAAGGACTTCTTTGAAAGTAATG TGCTTCAGCTTGATCCTTCACTATTAACTGAAAATGGAATGAAATGTTTTGAGAGATTCTTCAAAGCTGT GAATTGTCGAGAAGGAAAACTAGTAGCAAAAAGAAGAGCCTATATGATGGATGATTTGGAATTGATAGGA TTAGACTATCTTTGGAGGGTTGTAATTCAGAGTAATGATGATATTGCCAGCCGAGCTATTGATCTCCTCA AAGAGATATACACAAACCTTGGTCCAAGACTGCAGGTCAATCAGGTGGTGATCCATGAAGACTTCATTCA GTCTTGCTTTGATCGTTTGAAAGCCTCTTATGACACGTTGTGTGTTTTGGATGGTGACAAAGACAGTATT AATTGTGCAAGACAGGAAGCTGTTCGAATGGTCCGAGTATTAACTGTTTTAAGAGAATATATAAATGAAT GTGACAGTGATTATCATGAAGAAAGAACAATTTTGCCTATGTCAAGAGCTTTCCGTGGTAAACACCTCTC TTTTATAGTTCGGTTTCCAAACCAGGGCAGACAAGTAGATGACTTGGAAGTTTGGTCTCATACAAATGAT ACCATTGGTTCAGTTCGACGATGTATACTAAATCGCATTAAAGCCAATGTAGCTCATACAAAAATTGAAC TCTTTGTGGGTGGTGAGCTTATTGATCCTGGAGATGACAGAAAGTTGATTGGACAATTAAACCTAAAAGA TAAATCACTAATTACAGCCAAACTTACACAAATCAGCTCCAATATGCCGTCAAGTCCTGATAGCTCCTCT GATTCCTCAACTGGATCTCCTGGAAACCATGGTAATCATTACAGTGATGGTCCCAACCCAGAGGTAGAAA GCTGTTTGCCTGGAGTGATAATGTCACTTCATCCCAGATACATCTCTTTCCTTTGGCAAGTTGCAGACTT AGGGAGCAGCCTAAATATGCCCCCTCTTAGAGATGGAGCAAGAGTTCTTATGAAACTTATGCCGCCAGAT AGTACAACAATAGAAAAATTAAGAGCTATTTGTTTAGATCATGCCAAACTTGGAGAAAGCAGCCTTAGTC CATCTCTTGACTCACTTTTCTTTGGTCCTTCAGCCTCACAAGTGCTATACCTAACAGAGGTAGTCTATGC CCTGTTAATGCCTGCTGGTGCACCCCTGGCTGATGACTCCTCTGATTTTCAGTTTCACTTCTTGAAAAGT GGTGGTTTGCCCCTTGTTCTGAGTATGCTAACCAGAAATAACTTCCTACCAAATGCTGATATGGAAACTC GAAGGGGTGCCTACCTCAATGCTCTTAAAATAGCCAAACTGTTACTAACTGCCATTGGCTATGGCCATGT TCGGGCTGTGGCAGAAGCATGTCAGCCAGGTGTAGAAGGCGTGAATCCTATGACATCGGTCAACCAAGTA ACTCATGATCAAGCAGTGGTGCTACAAAGTGCCCTTCAGAGCATTCCTAACCCATCATCTGAATGCATGC TTAGAAACGTGTCTGTTCGTCTTGCTCAGCAGATTTCTGATGAGGCTTCAAGATATATGCCTGATATTTG TGTAATTAGAGCTATACAAAAGATTATTTGGACATCAGGATGTGGGGGATTACAACTGGTATTCAGCCCA AATGAGGAAGTCACAAAAATTTATGAGAAGACCAATGCAGGCAATGAGCCAGACCTTGAAGATGAACAGG TTTGCTGTGAGGCATTGGAAGTGATGACACTGTGTTTCGCCCTGATTCCAACAGCCTTAGATGCTCTAAG TAAAGAAAAGGCTTGGCAGACATTTATTATTGACTTACTGTTACACTGTCACAGCAAAACTGTCCGTCAA GTAGCACAGGAACAATTCTTCTTAATGTGCACCAGATGTTGCATGGGACACAGGCCTCTACTTTTCTTCA TTACTCTACTCTTTACTGTTTTGGGGAGCACAGCCAGAGAGAGGGCTAAACATTCAGGCGACTACTTTAC TCTTTTAAGGCATCTTCTTAATTACGCTTACAACAGTAACATTAATGTACCCAATGCTGAAGTTCTTCTA AATAATGAAATTGATTGGCTTAAAAGAATTAGGGATGATGTTAAAAGAACAGGTGAAACAGGCGTTGAAG AGACAATCTTAGAAGGACACCTTGGGGTTACAAAGGAGCTACTGGCTTTTCAGACCCCTGAGAAAAAGTT TCATATTGGTTGTGAAAAGGGGGGTGCTAATCTCATTAAAGAATTAATCGACGATTTCATCTTTCCTGCA TCCAATGTTTACCTCCAGTATATGAGAAATGGAGAACTCCCTGCTGAGCAGGCCATTCCTGTCTGTGGTT CACCAGCCACAATTAATGCTGGTTTTGAGTTACTTGTAGCATTAGCTGTTGGCTGTGTAAGGAACCTCAA ACAGATAGTAGATTCTTTGACTGAAATGTATTACATTGGTACAGCAATAACTACTTGTGAGGCACTTACT GAGTGGGAATACCTGCCACCTGTTGGACCCCGTCCACCAAAAGGATTTGTGGGGCTGAAAAATGCTGGTG CTACTTGTTACATGAATTCTGTGATTCAGCAACTCTATATGATTCCCTCTATCAGGAACGGTATTCTTGC AATTGAAGGCACAGGTAGTGATGTAGATGATGATATGTCTGGGGATGAGAAGCAGGACAATGAGAGCAAT GTTGATCCCAGGGATGATGTGTTTGGATATCCTCAACAATTTGAAGACAAACCACCACTGAGTAAAACAG AAGATAGAAAAGAGTACAATATTGGTGTTCTAAGACACCTTCAAGTTATCTTTGGCCATTTAGCTGCTTC TCGACTACAGTACTATGTGCCCAGAGGATTTTGGAAACAGTTCAGGCTTTGGGGTGAGCCTGTTAATCTT CGTGAACAACATGATGCTTTAGAGTTCTTTAATTCATTGGTGGATAGTTTAGATGAAGCTTTAAAAGCCT TAGGGCATCCAGCTATGCTAAGTAAAGTCTTGGGAGGTTCCTTCGCTGATCAGAAAATTTGTCAAGGCTG CCCACATAGGTATGAATGTGAAGAATCTTTTACGACTCTGAATGTAGACATTAGAAACCACCAAAATCTT CTTGATTCTTTGGAACAGTATGTCAAAGGAGATTTACTAGAAGGGGCAAATGCTTATCATTGTGAAAAAT GCAATAAAAAGGTTGATACTGTAAAGCGCTTGCTAATTAAAAAGTTGCCTCCTGTTCTTGCTATCCAACT AAAACGATTCGATTATGACTGGGAAAGAGAATGTGCAATCAAGTTCAATGATTACTTTGAGTTTCCTCGA GAGTTAGACATGGAGCCGTACACAGTTGCAGGTGTTGCTAAACTAGAGGGAGATAATGTCAACCCAGAAA GTCAGCTGATACAACAGAATGAGCAGTCTGAAAGCGAAAAAGCCGGAAGCACAAAATACAGACTTGTGGG TGTGCTTGTACACAGTGGTCAAGCAAGTGGGGGACATTACTATTCTTACATCATTCAGAGGAATGGAGGA GATGGTGAAAAAAATCGTTGGTATAAATTTGATGATGGAGATGTAACAGAGTGTAAAATGGATGATGATG AAGAAATGAAAAACCAGTGTTTTGGTGGCGAGTACATGGGAGAAGTGTTTGATCACATGATGAAGCGCAT GTCGTACAGGCGGCAGAAAAGGTGGTGGAATGCATATATACTTTTTTATGAAAGAATGGATACGATAGAT CATGATGATGAGGTGATAAGATACATATCAGAGATTGCTATCACCACAAGGCCCCATCAGATTGTTATGC CATCGGCCATTGAAAGAAGTGTGCGGAAGCAGAATGTCCAATTCATGCATAACCGAATGCAGTACAGTTT GGAATATTTTCAGTTTATGAAAAAGCTACTTACATGTAATGGTGTTTATTTAAACCCTCCACCAGGGCAA GATCACCTGTCTCCTGAAGCAGAAGAAATCACTATGATTAGTATTCAGCTTGCTGCTAGGTTCCTTTTTA CTACAGGATTTCACACAAAGAAAATAGTCCGAGGATCCGCCAGTGATTGGTATGATGCATTGTGTATTCT CCTCCGTCACAGCAAGAATGTGCGGTTTTGGTTTGCTCACAATGTGCTTTTTAATGTTTCAAATCGATTC TCTGAGTACCTTTTGGAATGCCCTAGTGCAGAAGTGAGAGGTGCATTTGCTAAACTTATAGTTTTCATTG CACACTTTTCCTTACAAGATGGGCCTTGTCCTTCACCTTTTGCATCTCCTGGACCTTCTAGTCAGGCTTA TGATAACTTAAGTTTGAGTGACCACCTACTAAGAGCAGTACTAAATCTCTTGAGGAGGGAGGTTTCAGAG CATGGACGTCATTTACAGCAGTATTTCAACTTGTTTGTAATGTATGCCAATTTAGGAGTGGCAGAGAAAA CACAGCTGCTCAAACTGAGTGTACCTGCTACCTTTATGCTTGTGTCCTTAGATGAAGGTCCTGGTCCTCC AATTAAATATCAGTATGCTGAATTAGGCAAATTATACTCCGTAGTGTCACAGTTAATCCGCTGTTGCAAT GTCTCTTCAAGAATGCAGTCTTCAATCAATGGTAATCCTTCTCTTCCAAATCCTTTTGGTGATCCTAATT TATCACAACCTATAATGCCAATTCAACAAAATGTGGTAGACATTTTATTTGTGAGAACAAGTTATGTGAA GAAAATTATTGAAGACTGCAGTAACTCTGATGAGACCGTCAAATTGCTTCGCTTTTGCTGCTGGGAGAAT CCTCAGTTCTCATCTACTGTCCTGAGTGAACTTCTTTGGCAGGTTGCGTATTCCTATACTTACGAACTCC GGCCCTATTTGGATCTGCTTTTACAAATCTTACTGATTGAAGACTCCTGGCAGACTCACAGAATTCATAA TGCCCTTAAAGGAATTCCAGATGACCGAGATGGGCTGTTTGACACAATTCAGCGTTCTAAGAATCACTAT CAAAAAAGAGCATACCAATGTATAAAATGTATGGTAGCCCTCTTTAGCAGTTGTCCTGTGGCTTACCAAA TCCTGCAGGGCAATGGAGATCTTAAAAGGAAATGGACCTGGGCAGTAGAGTGGCTTGGAGATGAACTCGA AAGAAGACCATACACTGGCAATCCTCAGTACACTTACAACAACTGGTCTCCTCCAGTGCAAAGCAATGAA ACATCAAATGGCTACTTCTTGGAGAGATCACATAGCGCTAGGATGACACTTGCAAAAGCTTGTGAACTCT GTCCTGAGGAGGAACCAGATGACCAAGATGCCCCAGATGAGCATGAATCACCTCCACCTGAAGATGCCCC ATTATACCCCCATTCCCCTGGATCTCAATATCAGCAGAATAACCATGTGCATGGACAGCCCTATACAGGC CCAGCCGCACATCACATGAACAACCCTCAGAGAACTGGCCAACGAGCACAAGAAAATTATGAAGGCAGTG AAGAAGTGTCCCCACCTCAGACCAAGGATCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200045 representing NM_001135923 Red=Cloning site Green=Tags(s) MTATTRGSPVGGNDNQGQAPDGQSQPPLQQNQTSSPDSSNENSPATPPDEQGQGDAPPQIEDEEPAFPHT DLAKLDDMINRPRWVVPVLPKGELEVLLEAAIDLSKKGLDVKSEACQRFFRDGLTISFTKILTDEAVSGW KFEIHRCIINNTHRLVELCVAKLAQDWFPLLELLAMALNPHCKFHIYNGTRPCESVSSSVQLPEDELFAR SPDPRSPKGWLVDLLNKFGTLNGFQILHDRFINGSALNVQIIAALIKPFGQCYEFLTLHTVKKYFLPIIE MVPQFLENLTDEELKKEAKNEAKNDALSMIIKSLKSLASRVPGQEETVKNLEIFRLKMILRLLQISSFNG KMNALNEVNKVISSVSYYTHRHGSSEEEEWLTAERMAEWIQQNNILSIVLRDSLHQPQYVEKLEKILRFV IKEKALTLQDLDNIWAAQAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCFKASWTNASKKQREKLLEL IRRLAEDDKDGVMAHKVLNLLWNLAHSDDVPVDIMDLALSAHIKILDYSCSQDRDTQKIQWIDRFIEELR TNDKWVIPALKQIREICSLFGEAPQNLSQTQRSPHVFYRHDLINQLQHNHALVTLVAENLATYMESMRLY GRDNEDYDPQTVRVGSRYSHVQEVQERLNFLRFLLKDGQLWLCAPQAKQIWKCLAENAVYLCDREACFKW YSKLMGDEPDLDPDINKDFFESNVLQLDPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMDDLELIG LDYLWRVVIQSNDDIASRAIDLLKEIYTNLGPRLQVNQVVIHEDFIQSCFDRLKASYDTLCVLDGDKDSI NCARQEAVRMVRVLTVLREYINECDSDYHEERTILPMSRAFRGKHLSFIVRFPNQGRQVDDLEVWSHTND TIGSVRRCILNRIKANVAHTKIELFVGGELIDPGDDRKLIGQLNLKDKSLITAKLTQISSNMPSSPDSSS DSSTGSPGNHGNHYSDGPNPEVESCLPGVIMSLHPRYISFLWQVADLGSSLNMPPLRDGARVLMKLMPPD STTIEKLRAICLDHAKLGESSLSPSLDSLFFGPSASQVLYLTEVVYALLMPAGAPLADDSSDFQFHFLKS GGLPLVLSMLTRNNFLPNADMETRRGAYLNALKIAKLLLTAIGYGHVRAVAEACQPGVEGVNPMTSVNQV THDQAVVLQSALQSIPNPSSECMLRNVSVRLAQQISDEASRYMPDICVIRAIQKIIWTSGCGGLQLVFSP NEEVTKIYEKTNAGNEPDLEDEQVCCEALEVMTLCFALIPTALDALSKEKAWQTFIIDLLLHCHSKTVRQ VAQEQFFLMCTRCCMGHRPLLFFITLLFTVLGSTARERAKHSGDYFTLLRHLLNYAYNSNINVPNAEVLL NNEIDWLKRIRDDVKRTGETGVEETILEGHLGVTKELLAFQTPEKKFHIGCEKGGANLIKELIDDFIFPA SNVYLQYMRNGELPAEQAIPVCGSPATINAGFELLVALAVGCVRNLKQIVDSLTEMYYIGTAITTCEALT EWEYLPPVGPRPPKGFVGLKNAGATCYMNSVIQQLYMIPSIRNGILAIEGTGSDVDDDMSGDEKQDNESN VDPRDDVFGYPQQFEDKPPLSKTEDRKEYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFRLWGEPVNL REQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQKICQGCPHRYECEESFTTLNVDIRNHQNL LDSLEQYVKGDLLEGANAYHCEKCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPR ELDMEPYTVAGVAKLEGDNVNPESQLIQQNEQSESEKAGSTKYRLVGVLVHSGQASGGHYYSYIIQRNGG DGEKNRWYKFDDGDVTECKMDDDEEMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERMDTID HDDEVIRYISEIAITTRPHQIVMPSAIERSVRKQNVQFMHNRMQYSLEYFQFMKKLLTCNGVYLNPPPGQ DHLSPEAEEITMISIQLAARFLFTTGFHTKKIVRGSASDWYDALCILLRHSKNVRFWFAHNVLFNVSNRF SEYLLECPSAEVRGAFAKLIVFIAHFSLQDGPCPSPFASPGPSSQAYDNLSLSDHLLRAVLNLLRREVSE HGRHLQQYFNLFVMYANLGVAEKTQLLKLSVPATFMLVSLDEGPGPPIKYQYAELGKLYSVVSQLIRCCN VSSRMQSSINGNPSLPNPFGDPNLSQPIMPIQQNVVDILFVRTSYVKKIIEDCSNSDETVKLLRFCCWEN PQFSSTVLSELLWQVAYSYTYELRPYLDLLLQILLIEDSWQTHRIHNALKGIPDDRDGLFDTIQRSKNHY QKRAYQCIKCMVALFSSCPVAYQILQGNGDLKRKWTWAVEWLGDELERRPYTGNPQYTYNNWSPPVQSNE TSNGYFLERSHSARMTLAKACELCPEEEPDDQDAPDEHESPPPEDAPLYPHSPGSQYQQNNHVHGQPYTG PAAHHMNNPQRTGQRAQENYEGSEEVSPPQTKDQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001135923
ORF Size	7662 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001135923.1, NP_001129395.1
RefSeq Size	12133 bp
RefSeq ORF	7665 bp
Locus ID	363445
MW	290.2 kDa

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