Megf8 (NM_053628) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Egfl4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217791 representing NM_053628 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTTGGGCGGAGCTCTGGCCGCGGCCCTTGCACTGGCCTTCGCTGTGCTGGGGCCGCTGTCCCACA AGGTCCTGGCCGGTGACTGCAAGGGGCAGCGGCAGGTGCTACGGGAGGCACCAGGCTTCGTGACGGATGG TGCGGGCAACTACAGCGTCAATGGCAACTGCGAGTGGCTTATCGAGGCACCGAGCCCTCAGCACCGGATC CTGCTGGACTTCCTTTTCCTGGACACAGAGTGCACTTATGACTACCTCTTTGTGTATGATGGTGACTCAC CCCAAGGCCCGCTGCTTGCTAGTCTGAGTGGGAGCACCCGGCCCCCACCCATCGAGGCATCCTCAGGCAA GATGCTGCTCCATCTCTTCAGTGATGCCAACTACAACCTGCTGGGCTTTAACGCCTCCTTCCGCTTCTCC CTGTGTCCTGGAGGTTGCCAGAACCACGGGCAGTGCAAGTCTCCAGGAGTGTGTGTCTGCGAGCCAGGCT GGGGGGGTCCAGACTGTGGCCTGCAGGAGTGCTCAGCCTACTGTGGTAGCCACGGTACCTGTGCCTCGAC CCTGGGACCATGCCGCTGTGAGCCTGGATTCCTGGGACGGGCCTGTGACCTGCACTTGTGGGAGAACCAG GGGGCTGGCTGGTGGCACAGTGTGAGTGCCGGGGACCCCGCCTTCTCTGCCCGAGTTGGGGCAGCTGGTG CCTTCCTGTCTCCACCAGGGCTGCTGGCTGTCTTTGGAGGTCAGGACCTTAACAAGGCCCTGGGGGATCT TGTCCTGTACAACTTCTCCACCAACACCTGGGAATCCTGGGATCTGACGCCTGCTCCGGCCGCCCGTCAC TCCCACGTGGCCGTGGCCTGGGCCGGCTTCCTGGTGCTGATGGGTGGTGAGCTGGCCAATGGCTTGCTAA CCAACGATGTGTGGGCCTTTAGCCCCCTGGGTGGAGGCCACTGGGAGCTCCTGGCACCACCTGCTTCCAG TTCCTCTGGGCCCCCAGGGCTGGCAGGCCATGCAGCAGCTCTGGTAGATGACATCTGGCTGTATGTGTCC GGTGGCCGCACTCAGCATGACCTCTTTTCTTCTGGCCTCTTCCGCTTCCGCCTGGACCACACCAGCAGGG GCTACTGGGAGCAGGTGATCCCTGCAGGTGGGCGGCCCCCTGCTGCCACTGGCCACTCTATGGTGTTCCA CGCCCCGTCCCGTACCCTGCTGGTCCATGGTGGACACCGGCCTTCCACTGCCCGGTTTTCCGTGCGGGTG AATTCCACCGAGCTCTTCCACGTGGACCGCCGTGTGTGGACCACGCTGAAGGGCCGGGATGGGCTCCAGG GTCCACGGGAGAGAGCCTTCCACACAGCCAGTGTTCTAGGAAACTACATGGTGGTCTATGGGGGCAATGT GCACACCCATTACCAGGAGGAAAAGTGTTACGAAGATGGCATCTTCTTCTACCACCTTGGTTGCCACCAA TGGGTGTCGGGGGCCGAACTTGCCCCACCAGGGACCCCTGAAGGTCGAGCAGCGCCCCCTAGTGGTCGTT ACTCACATGTGGCAGCTGTTCTCGGTGGCAGTGTCCTGCTGGTGGCTGGGGGATACAGTGGCCGGCCTCG TGGGGACTTGATGGCCTACAAGGTGCCCCCCTTCGTGTTTCAGGCACCCGCCCTGGATTACCACTTGGAC TACTGTTCCATGTACACAGACCACAGTGTGTGCTCCCGGGACCCCGAATGTAGCTGGTGTCAGGGTGCTT GCCAATCCGCACCCCCTCCCGGGACTCCCTCAGGAGCTTGTCCCGCAGCCAGCTGCCTGGGCCTAGGCCG CCTACTGAGTGACTGCCAGGCATGCCTGGCCTTCAGCAGCCCCACGGCTCCTCCCCGAGGACCTGGCACC CTAGGTTGGTGTGTGCACAACGAGAGCTGCCTTCCCCGGCCAGAGCAGGCACGCTGCCGAGGGGAGCAGA TCTCAGGCACTGTGGGCTGGTGGGGGCCAGCACCCGTCTTTGTCACTTCCCTGGAGGCTTGTGTCACCCA GAGCTTCCTCCCTGGCCTGCACCTGCTCACCTTCCAGCAGCCACCCAACGCCTCTCAGCCTGACAAGGTC TCCATTGTCCGAAGTACAACTATCACCTTGACCCCCAGCGCGGAGACAGATGTGTCCCTGGTCTACCGAG GCTTCATCTACCCGATGCTGCCAGGAGGACCAGGTGGCCCTGGGGCCGAAGATGTGGCTGTGTGGGCTCG GGCCCAGCGCCTCCATGTCCTGGCCCGGATGGCCAGAGGCCCTGACACTGAGAACATGGAAGAGGTGGGA CGCTGGGTGGCTCAGCAGGAGAAGGAGACACGGCGTCTGCAGCGCCCCGGCTCTAGTCGCCTCTTCCCAC TGCCCGGTCGAGGCAACAAGTATGCTGTGGAGATCCGAGGCCAGCTCAATGGCTCCGCAGGCCCTGGGCA CAGTGAGCTCACTCTCCTGTGGGATCGGACTGGAGTGCCGGGTGGCAGTGAGATCTCCTTCTTCTTCCTG GAGCCCTACCGCTCTTTGGCCTGCTCCTCCTACTCCTCCTGTCTGGGCTGCCTGGCCGATCAGGGCTGTG GCTGGTGCCTCAACAGTGCCACCTGTCACCTGCGTCAGGGCAGGGCCCACTGTGAGGATGACGGGAACGG CGAGTCCTTGCTGGTGCTGGTGCCTGCCCTCTGTCCACTCTGTGAGGAGCATCGTGACTGCCATGCCTGC ACCCAGGACCCCTTTTGTGAGTGGCATCAGAGCACCAACCGCAAAGGTGACGCAGCCTGCAGCCGGCGAG GCCGGGGCCGAGGCGCTCTGAAGAATCCCGAGGAATGCCCCCCACTCTGCAGCCAACGCCTGACCTGTGA GGACTGCCTGGCCAACTCTAGCCAGTGCGCCTGGTGCCAGTCAACTCACACCTGCTTCCTGTTTGCTGCC TACCTGGCCCGGTACCCACACGGGGGCTGCCGTGGCTGGGACGACAGTGTGCATTCAGAGCCACGCTGCA GGAGCTGCCACGGATTTTTGACTTGTCACGAGTGTCTACAGAGCCACGAGTGTGGTTGGTGTGGCAATGA AGACAACCCCACTCTGGGACGGTGCCTACAGGGGGACTTCTCCGGACCCCTGGGTGGGGGGAACTGCTCC CTGTGGGTGGGAGAGGGCCTGGGGCTGCCTGTGGCTCTTCCTGCTCGCTGGGCTTATGCCCGCTGTCCAG ATGTGGATGAATGTCGCCTGGGTCTGGCGAGGTGTCACCCACGGGCAACGTGCCTCAACACGCCTCTCAG CTATGAGTGTCATTGCCAGCGCGGCTACCAGGGTGATGGCATCACACACTGTAACCGCACGTGCCTGGAA GACTGTGGCCACGGTGTGTGTAGCGGTCCCCCGGACTTCACTTGCGTGTGTGACCTGGGTTGGACCTCAG ATTTGCCACCACCCACACCTGCCCCAGGACCCCCTGCCCCACGCTGCTCTCGAGACTGTGGCTGCAACTT TCACAGTCATTGCCGCCGGCGGGGACCTGGCTACTGTGATGAGTGCCAGGACTGGACATGGGGGGAACAC TGTGAACGGTGCCGGCCTGGCAGCTTTGGCAATGCAACGGGCTCTGGTGGCTGCCGACCCTGCCAGTGTA ACGGGCATGGAGACCCACGTCGTGGCCACTGTGACAACCTCAGTGGGCTCTGCTTCTGCCAGGACCATAC TGAAGGTGCCCACTGTCAGATTTGCTCTCCAGGATACTATGGGGACCCCCGAGCTGGTGGCTCCTGCTTC CGGGAGTGTGGGGGTCGTGCCCTCCTCACCAATGTGTCCTCAGTGGCTCTGGGCTCTCGCCGCTTTGGGG GACTTCTGCCTCCAGGTGGTGGGACAGCAAGAGCTGGACCCGGCCTGTCCTACTGTGTGTGGGTTGTCTC AGCCACAGAAGCGCTGCAGCCCTGTGCCCCTGGGACCCTCTGTCCCCCACTCACCCTCACCTTCTCTCCA GACAGCAGCACCCCTTGCACACTGAGCTATGTCCTGGCCTTTGATGGATTCCCACGCTTTCTGGACACTG GGGTCGTCCAGTCAGACCGGAGCCTCATAGCTGCCTTCTGTGGTCAGCGGCGGGACAGACCTCTCACCGT GCAGGCTTTATCTGGGTTACTTGTGTTGCACTGGGAGGCCAATGGCTCCTCCTCCTGGGGATTCAATGCT TCCGTGGGCTCTGCCCGATGTGGATCAGGAGGCCCTGGCAGTTGCCCTGTCCCTCAGGAGTGTGTGCCCC AGGATGGGGCTGCAGGTGCTGGGCTCTGCCGATGTCCTCAGGGCTGGGCTGGGCCTCACTGCCGCATGGC TCTGTGTCCTGAGAACTGCAACGCCCACACGGGGGCAGGGATTTGTAACCAGAGCCTAGGTGTGTGCATC TGTGCTGAGGGCTTTGGTGGCCCAGACTGTGCCACGAAGCTGGATGGTGGGCAGTTGGTCTGGGAAACAC TCATGGACAGCCGCCTCTCAGCTGACACTGCTAGTCGCTTCCTTCACCGCCTGGGGCATACTATGGTAGA GGGACCTGATGCTACTTTGTGGATGTTTGGGGGACTGGGTCTGCCGCAGGGCCTGCTGGGAAACTTGTAT AGGTATTCAGTCAGTGAGCGCAGGTGGACACAGATGCTGGCAGGAGCTGAGGATGGGGGCCCAGGCCCAT CACCCCGCTCCTTTCACGCAGCTGCCTATGTACCTGCTGGCCGTGGTGCCATGTACCTGCTGGGGGGCCT CACAGCTGGGGGCATCACCTGTGACTTCTGGGTCCTTAACCTCACTACCCTACAGTGGAGACAGGAGAAG GCCCCTCAGAGCATAGAGCTGCCAGCAGTCGCTGGTCACACCCTTACTGCCCGTCGAGGCCTGTCTCTGC TCTTGGTGGGTGGCTACTCCCCTGAAAATGGCTTCAACCAGCAGTTGTTGGAGTACCAGCTGGCAACCGG CACGTGGGTGTCTGGAGCCCAGAGTGGGACGCCCCCAACAGGTCTCTATGGCCATTCTGCGGTCTACCAT GAGGCCACCGACTCCCTCTATGTGTTTGGGGGTTTCCGCTTCCATGTGGAATTGGCGGCGCCTTCTCCGG AGCTCTACTCCCTGCACTGTCCTGACCGCACCTGGAGCTTGCTGGCACCTTCTCAGGGGGCCAAGCCCCG GCCCCGGCTCTTCCATGCCTCAGCCCTGCTGGGGGACACCATGGTGGTCCTCGGGGGGCGCTCGGACCCC GATGAATTCAGCAGTGATGTCCTGCTCTACCAGGTCAACTGCAACACCTGGCTCCTGCCTGACCTCACCC GTCCAGCCTTTGTGGGGTCCCCGATGGAGGAGTCCGTGGCTCACGCAGTGGCAGCAGTGGGTAGCCGCCT GTACATCTCTGGAGGATTTGGGGGAGTAGCCTTGGGCCGCCTGCTGGCATTAACCCTGCCCCCTGACCCC TGTCGTCTGCTGCCTTCACCTGAAGCTTGCAACCAGTCTGGGGCCTGCACCTGGTGCCATGGTGCTTGCC TGTCTGGGGACCAGGCCCACAGACTGGGTTGTGGGGTGCCCCCTTGCTCCCCAATGCCTCGCTCGCCTGA GGAATGTCGGAGACTGCGGACCTGCAGTGAGTGCCTGGCCCGCCATCCTCGGACCCTGCAGCCTGGAGAT GGAGAGGCATCCGTTCCCCGCTGTAAGTGGTGCACTAACTGCCCAGAGGGCGCCTGCATCGGGCGCAATG GGTCCTGCACCTCCGAGAATGACTGTCGAATCAACCAACGAGAGGTCTTCTGGGCTGGTAACTGCTCAGA GGCCGCATGCGGGGCTGCCGACTGTGAGCAGTGCACACGGGAGGGAAAGTGCATGTGGACTCGGCAATTC AAGAGGACTGGGGAGACTCGGCGTATCCTGTCAGTGCAGCCCACCTACGACTGGACTTGCTTCAGCCATT CTCTGCTGAATGTGTCTCCTATGCCGGTGGAGTCCTCACCCCCACTGCCCTGTCCCACCCCCTGTCACCT CCTCCCCAATTGCACATCCTGCCTGGCCTCCAAGGGTGCAGATGGAGGCTGGCAGCACTGTGTGTGGAGC AGCAGCCTCCAGCAGTGTCTGAGCCCCTCCTACCTGCCCTTACGCTGTATGGCTGGAGGCTGTGGGCGGC TGCTTCGGGGGCCAGAGAGCTGCTCCCTGGGCTGTGCTCAGGCTACGCAGTGCGCCCTGTGTCTGCGAAG ACCCCACTGTGGCTGGTGTGCCTGGGGGGGACAGGATGGAGGTGGCCACTGCATGGAAGGTGGTCTCAGT GGCCCCCGAGATGGGCTCACGTGTGGACGTCCTGGGGCATCCTGGGCCTTCCTGTCCTGCCCCCCTGAGG ACGAGTGTGCAAACGGGCACCATGACTGCAATGAGACTCAGAACTGCCACGACCAGCCCCACGGCTACGA GTGCAGCTGCAAGACAGGCTACACCATGGACAATGTGACTGGCGTCTGCCGCCCTGTGTGTGCCCAGGGC TGTGTGAACGGCTCCTGTGTGGAGCCTGATCACTGCCGGTGTCACTTTGGCTTTGTTGGCCGCAATTGCT CCACAGAGTGTCGTTGCAATCGCCACAGTGAGTGTGCTGGCGTTGGAGCACGGGATCACTGCCTGCTGTG CCGGAACCACACCAAGGGTAGCCACTGTGAGCAGTGTCTCCCACTGTTTGTGGGTTCGGCTTTGGGTGGC GGGACCTGCCGGCCATGCCATGCCTTCTGTCGAGGGAACAGCCATGTGTGTGTCTCCAGGAAAGAGCTAG AAATGGCCAGAAGGGAGCCAGAGAAGTACTCACTGGATCCAGAGGAGATTGAAGCCTGGGTGGCAGAAGG GCCTAGTGAAGATGAAGCTGTGTGTGTGAACTGCCAGAACAACAGCTACGGAGACAGATGTGAGAGCTGC CTGCACGGCTACTTCCTCCTGGACGGGAAATGCACCAAGTGCCAGTGCAATGGCCACGCTGACACCTGTA ACGAGCAGGACGGAACAGGATGCCCATGTCAGAACAACACAGAGACAGGCGTCTGCCAGGGCAGCTCTCC TAGTGACCGCCGAGACTGCTACAAGTACCAGTGTGCTAAGTGCCGGGAGTCATTCCACGGGAGCCCACTG GGTGGCCAGCAGTGTTACCGACTCATCTCGGTGGAGCAGGAGTGCTGCCTGGACCCCACTTCCCAGACCA ACTGCTTCCATGAGCCGAAGCGCCGGGCCTTGGGTCCTGGCCGAACTGTGCTGTTTGGCGTCCAACCCAA GTTCACCAATGTGGACATCCGCCTGACACTAGACGTGACCTTCGGTGCTGTGGACCTCTATGTCTCCACT TCCTATGACACTTTTGTGGTCCGAGTGGCCCCTGACACAGGTGTCCACACTGTGCACATCCAGCCACCCC CACCTCCCCCACCTCCTCCGCCTCCTGCCGATGGTGTACCTCGTGTGGCTTCGGACCTAGGAGGACTGGG GACCGGAAGTGGATCAGGTTCTCCAGTGGAGCCACGAGTCCGCGAGGTGTGGCCACGGGGCCTGATTACC TATGTGACAGTGACGGAACCCTCGGCAGTGCTGGTGGTCCGCAGTGTGCGGGACAGGCTGGTGATCACCT ATCCCCATGAGCACCACGCCCTCAAGTCCAGCCGCTTCTACCTGCTGCTGCTGGGGGTAGGGGATCCCAA CGGGCCTGGGGCCAACGGCTCGGCGGACTCACAGGGCCTGCTCTTCTTCCGGCAAGACCAGGCCCACATT GACCTGTTCGTCTTCTTCTCTGTCTTCTTTTCCTGCTTCTTCCTCTTCCTCTCACTCTGTGTGCTCCTCT GGAAGGCCAAGCAGGCTCTGGACCAGCGGCAAGAGCAGCGCCGGCACCTGCAGGAGATGACCAAGATGGC TAGCCGCCCCTTTGCCAAGGTCACAGTCTGCTTCCCTCCAGACCCTGCGGGCCCAGCACCTGCCTGGAAG CCAGCTGGCCTCCCGCCTCCTGCCTTCCGCCGCTCCGAGCCATTTCTTGCACCTCTACTGCTGACAGGAG CTGGGGGGCCCTGGGGACCCATGGGAGGAGGGTGCTGCCCACCAGCTCTCCCTGCCACCACAGCTGGCCT ACGAGCTGGGCCCATTACCCTTGAACCCACAGAAGATGGCATGGCTGGTGTGGCCACTCTGCTGCTCCAG CTACCTGGGGGGCCTCATGCTCCCAATGGGGCCTGCCTTGGGTCAGCCCTTGTCACTCTGCGTCATAGGC TGCATGAGTACTGTGGGGGAAGCGGGGGTGCTGGGGGCAGTGGACATGGGGGTGGTGGAGGCCGGAAGGG ACTGTTAAGTCAGGACAATCTCACTAGCATGTCCCTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053628
Insert Size	8370 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053628.2, NP_446080.1
RefSeq Size	11924 bp
RefSeq ORF	8370 bp
Locus ID	114029

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