Unc79 (NM_001271253) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1311117
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217776 representing NM_001271253 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCTCAACGCTAGCCACCTTCCCTCCATTTCTCCACAAGGATATTATCGAATATCTTAGCACATCTT TTCTACCGATGGCTATATTGGGGTCCTCCAGGAGAGAAGGTGTTCCGGCCCATGTTAACCTCTCTGCGTC CTCCATGCTGATGATCGCGATGCAGTACACCTCTAACCCAGTGTATCATTGTCAATTGTTGGAATGCCTC ATGAAGTATAAACAAGAAGTCTGGAAAGATCTTTTGTATGTGATTGCCTATGGGCCTTCCCAGGTGAAGC CTCCCGCAGTGCAGATGCTTTTCCACTATTGGCCCAACCTGAAGCCTCCCGGAGCAATAAGCGAGTACAG GGGCTTACAATACACAGCTTGGAATCCCATCCACTGCCAGCACATTGAATGTCACAATGCAATCAACAAG CCGGCTGTGAAGATGTGTATAGACCCGTCCTTGTCAGTAGCTTTGGGCGATAAGCCACCTCCACTGTATC TCTGCGAAGAATGCAGCGAGAGGATTTCAGGGGACCACAGCGAGTGGTTGATTGATGTTCTCCTGCCACA AGCTGAAATATCTGCTATATGTCAGAAAAAGAACTGCAGTTCCCATGTCAGAAGGGCAGTGGTCACCTGC TTTTCCGCAGGGTGCTGCGGTCGCCACGGAAACAGGCCTGTTCGGTACTGCAAAAGGTGCCATTCGAACC ACCACAGTAACGAGGTGGGGGCCACCGCAGAGACTCACCTCTACCAGACCTCTCCGCCCCCCATCAACAC CCGGGAGTGTGGGGCGGAGGAGCTGGTCTGCGCTGTGGAGGCAGTGATCAGCTTGTTGAAGGAAGCGGAG TTCCACGCAGAGCAGAGGGAGCACGAGTTGAACCGCCGGCGGCAGCTGGGCCTCTCTTCCTCCCACCACT CCCTGGACAACACTGACTTTGATAACAAAGACGATGACAAACACGACCAGAGGCTGCTCAGTCAGTTCGG GATATGGTTCTTAGTGAGCCTCTGCACACCCAGCGAGAACACGCCCACGGAGAGCCTGGCCCGGCTGGTA GCCATGGTATTTCAATGGTTTCACTCCACGGCGTACATGATGGATGATGAGGTCGGAAGCTTGGTGGAAA AGCTGAAGCCACAGTTTGTCACCAAGTGGTTGAAGACAGTATGTGATGTCCGATTTGATGTCATGGTCAT GTGCCTTCTTCCCAAACCCATGGAATTTGCCAGGGTTGGCGGATACTGGGACAAGTCCTGTAGCACGGTG ACTCAGTTAAAGGAAGGGCTCAACAGGATCCTCTGCCTGATCCCTTACAATGTCATCAGCCAGTCTGTGT GGGAGTGCATTATGCCTGAGTGGCTGGAAGCCATCCGGACAGAAGTCCCTGATAACCAGTTAAAGGAATT CAGGGAAGTATTAAGCAAAATGTTTGACATAGAGCTGTGCCCTCTGCCATTTTCAATGGAAGAGATGTTC GGCTTTATTAGCTGTCGGTTTACAGGATATCCCTCCACTGTGCAGGAGCAAGCGCTACTCTGGCTTCATG TACTTTCGGAGTTAGATATCACGGTTCCACTCCAGCTCTTGATAAGTATGTTTTCTGATGGTGTTAATTC TGTCAAAGAATTGGCAAATCAAAGAAAATCAAGAGCCAATGAACTGGCAGGGAACCTCGCATCTCGAAGG GTGAGTGTCGCCTCTGATCCTGGTCGACGAGGTCAGCACAATACCTTGAGTCCGTTTCATAGCCCTTTCC AGAGTCCGTTCCGGAGTCCCATGCGTAGTCCATTCCGTAGCCCCTTCAAGAACTTTGGACACCCAGGAGG AAGGACTATTGATTTTGATTGTGAAGATGATGACATGAATCTAAATTGTTTCATCCTCATGTTTGACCTT CTCCTAAAGCAGATGGACTTACAAGACGACGGCATCACAATGGGTTTGGAGCACAGTTTGTCAAAGGACA TTATTTCTATCATAAACAATGTCTTCCAGGCCCCTTGGGGGGGCTCCCACAGCTGCCAGAAAGACAAAAA GGCGACCGAGTGTAGCCTGTGCCAGTCCAGCATCCTCTGTTACCAGCTCGCCTGTGAGCTCCTGGAGAGA CTGGCTCCCAAAGAGGAGAGCCGGCTGGTGGAGCCCACAGACAGCCTTGAAGATAGCCTCCTTTCTTCCA GACCAGAGTTCATTCTCGGCCCTGAGGGAGAGGAGGAGGAGAACCCAGCGGCCAAGCATGGAGAGAATCC AGGCAACCGTACAGTGCCCTCAGAACATGCTGCAATAAAGAACGACACGGAAAGAAAATTTTGCTACCAA CAGCTTCCAGTTACGTTGAGACTAATATATACCATTTTCCAGGAAATGGCGAAGTTCGAAGAGCCAGACA TTCTTTTTAATATGCTCAACTGCCTAAAGATTCTCTGTCTGCACGGGGAATGCTTGTACACTGCGAGGAA AGACCATCCTCAATTTTTAGCCTACATTCAGGACCACATGTTGATTGCAAGCCTCTGGAGAGTCGTCAAG TCTGAGTTCTCTCAGCTGTCTTCACTGGCGGTTCCTCTCCTTCTTCACGCTCTGTCCCTTCCTCATGGTG CTGACATATTCTGGACAATCATTAATGGCAACTTCAACAGCAAAGACTGGAAGATGAGGTTTGAAGCAGT GGAGAAAGTGGCTGTCATTTGTAGGTTTTTGGACATTCACTCAGTGACCAAGAACCACCTGCTGAAGTAT TCTCTGGCACATGCCTTCTGCTGCTTCCTGACAGCTGTGGAGGATGTCAATCCTGCAGTGGCCACCAGAG CAGGTCTCCTGCTTGACACCATAAAGAGGCCAGCACTGCAGGGTCTTTGTCTTTGCCTTGACTTCCAGTT CGACACTGTGGTTAAAGACCGACCCACCATCCTGAGCAAGCTTTTACTCCTGCACTTTCTTAAGCAAGAT ATTCCTGCTTTGAGCTGGGAGTTCTTTGTCAACAGATTTGAGACACTTTCTTTGGAAGCTCAGCTGCATT TAGATTGTAACAAAGAATTTCCTTTTCCTACAACCATCACTGCTGTGAGAACCAATGTTGCCAACCTCAG CGATGCTGCCTTGTGGAAGATCAAGAGAGCACGTTTTGCCAGAAACCGACAGAAGAGTGTGAGGTCCCTG AGAGACAGTGTGAAAGGGCCAGCAGAATCCAAGAGGGCGCTCTCCCTCCCTGAGACCCTGACCTCCAAAA TCCCTGTGAGGTTGACCAGGCATGAGCAGTCTGCCCCGGCTCTCGGTGGGACACCTGAACAGACGCCAGG GCAACAGTCACCGGAAAACGACAACACCATCAAGGACTTGCTCCCGGAAGATGCTGGCATTGATCACCAG ACAGTTCACCAGCTCATTACAGTGCTCATGAAGTTCATGGCCAGGGACGAAAGCAGCGCGGAGTCAGACA TCAGCAGCGCAAAGGCCTTCAACACAGTCAAGAGGCACCTCTATGTTCTACTTGGCTATGACCAACAGGA AGGATGCTTCATGATCGCACCCCAAAAAATGCGCCTGTCAACATGCTTTAACGCGTTCATTGCAGGAATT GCCCAAGTTATGGACTATAACATTAACTTGGGCAAACACCTTCTCCCCCTCGTGGTTCAGGTGCTCAAAT ACTGCTCTTGTCCTCAACTACGGCATTATTTCCAACAGCCGCCTCGCTGCTCCCTCTGGTCCCTAAAGCC TCATATCAGGCAGATGTGGTTGAAGGCCTTGCTTGTCATCCTTTACAAGTATCCATACAGAGACTGTGAT GTCAGCAAGACCCTGCTGCACCTGATTCACATAACAGTCAATACACTCAATGCCCAGTACCATAGCTGCA AGCCCCACGCCACGGCAGGACCTCTGTACACTGACAACAGCAACATAAGCAGATACAGCGAGAAAGAAAA AGAAGAAGATAGTGTTTTTGATGAATCTGATATTCATGATACACCTACTGGACCCTGCAATAAAGAGTCT CAAACTTTTTTTGCAAGATTGAAAAGGATAGGCGGCAGCAGGATGGTCAAAGGCCAGCCAGTTGGGATGT CTGTGCAGAGAAGTGAAATAGAGCTGTCCGAATATAGAGAAACGGGTGCGTTGCAAGACAGCGTTCTGCA CTGTGTGAGAGAAGAAAGCATTCAGAAAAAAAAGCTACGCTCTTTAAAACAAAAATCTCTTGATATAGGC AATGCAGACTCCCTCTTGTTTACGTTAGACGAACATCGCAGGAAGTCCTGCATAGATCGGTGTGACATAG ATAAGCCTCCCGCCCAAGCTGCTTATATCACACAACGGCAAAACGACCACCACGGACGTTCTAGACAGAA TTCTGCTACAAGGCCGGACAATACCGAAATCCCCAAGAATCCAGGCACCGAAGGCTTTCAGGAGACACGA AGGCCGGTGATACCAGAGGTTAGGTTGAACTGCATGGAGACCTTTGAAGTGAGAGTTGACTCCCCAGGAA AGCCTGCTCCTAGGGAGGATTTAGATCTGATAGATCTGTCCTCAGACTCAACATCTGGGCCAGAGAAGCA CTCTATACTGTCCACATCTGACAGTGACTCTCTTGTGTTTGAGCCTCTCCCGCCTCTCAGAATAGTCGAA AGTGACGAGGAAGAGATGATGAACCAAGGGAACGGCGGTCCCTTGGGTAACAATGCTGCCTCCTCCCCCT CCATTCCTAGCCAGCCCTCTGTCCTCAGCCTGAGCACAACTCCTCTCGTGCAGGTGAGCGTAGAGGACTG TTCCAAAGACTTTTCTTCGAAGGACTCAGGAAACCACCAATCAGTGAGCAATGAGGACTCTACTGTCACA GCTCTGGATGACCTCATGGACTCTGAAGAGCTGTCCAAGTCAGAGGAGCTGAGAGAGTTTATCTCTGGCA GCCCGCTAACGCTTAAGCAAAAACGAGACCTCCTTCAGAAGTCATTCGCTGTCCCTGAGACGTCAGTGGA CTATAACCCCGAACCCGGCCAGGCTGAAGAAAAGCCAACATCAGGGGCCAAAACTGTGCTCCTTAAAGTT CCTGAAGATGGTGAGAATCTAACAGAAAGTGAGAGGCCCAACCCCAGTGCTGAGTCTGACACAGAACAGA ACCCTGAAAGGAAGGTGGAGGAGGATGGAGCCGAGGAATCAGAATTTAAGATTCAGATCGTCCCCAGGCA GAGGAAACAAAGGAAGATTGCCGTCAGCGCTATCCAGAGAGAGTACCTTGACATCTCCTTCAATATCCTA GACAAACTGGGTGAACAGAAGGATCCAGATCCCTCTGCTAAAGGACTTTCAACGTTGGAAATGCCACGGG AATCTTCCTCTGCCCCTACGTTAGAAGCAGGTGCACCCGAAACAAGCAGCCACTCATCAATATCAACTCA GTATAGGCAGATGAAAAGGGGATCCCTGGGAGTTCTGACAATGAGCCAGTTAATGAAGCGACAGCTGGAG CATCAGTCTAGCGCCCCCCATAACATCAGCAGCTGGGACACTGAACAGATCCAGCCTGGGAAAGGGCAGT GTAACGTGCCAATGTGCCTAAACCCCGACCTGGAGGGACAGCCACTGCGAACGAGAGGCGCCACTAAATC CAGCCTGCTGTCAGCACCCAGTATCGCCAGCATGTTTGTGCCTGCACCTGAAGAGTTCACAGAGGAGCAG CCGACAGTGATGGCTGACAAATGCCATGACTGCGGGGCCATTCTCGAGGAATACGATGAGGAGACCCTGG GGCTAGCCATCGTGGTCCTCTCCACCTTCATTCACCTGAGCCCAGACTTGGCTGCGCCACTGTTGCTTGA TATCATGCAGTCTGTGGGAAGGCTCGCATCCAGCACTACGTTTTCTAATCAAGCAGAAAGCATGATGGTT CCAGGCAATGCGGCGGGTGTGGCCAAGCAGTTCCTGCGTTGTATCTTCCATCAGCTGGCCCCCAACGGCA TCTTCCCTCAGCTCTTCCAGAGTGCCATCAAAGATGGGACTTTTTTACGGACCTTAGCCACATCTCTCAT GGACTTCAACGAGCTGAGCTCAATCGCTGCCCTCAGTCAGCTCCTGGAGGGTCTAAATAACAAAAAGAAT TTACCAGCAGGGGGTGCTATGATACGCTGTTTGGAAAACATCGCCACGTTCATGGAAGCTCTGCCCATGG ATTCTCCTAGCAGCCTCTGGACCACGATCAGCAACCAGTTTCAGACATTTTTTGCCAAGCTGCCTTGTGT TTTACCTCTGAAGTGTTCTTTAGATTCCAGTTTGAGAATTATGATTTGCCTCTTGAAGATACCTTCTACC AATGCCACGAGGAGTTTGTTGGAGCCCTTCTCCAAGCTGCTGAGCTTTGTCATTCAGAATGCAGTCTTCA CGCTGGCCTACCTGGTGGAGCTGTGTGGCCTGTGCTACAGAGCCTTCACAAAGGAGCGGGATAAGTTTTA CTTGTCTCGGAGCGTTGTTCTGGAACTTCTGCAGGCCCTGAAGCTCAAGTCACCTTTGCCAGACACAAAC CTCCTGCTTCTTGTCCAGTTTATTTGTGCAGATGCTGGAACCAAGCTAGCTGAGTCCACCATCCTGAGCA AGCAGATGATAGCCTCTGTGCCCGGGTGTGGGACAGCAGCAATGGAATGCATACGGCAGTACGTCAGTGA GGTGCTGGACTTCATGGCAGACATGCACACCCTAACCAAGCTCAAGAGTCACATGAAGACGTGTTCCCAG CCTCTGCACGAGGACACCTTCGGGGGCCATCTCAAAGTTGGCCTGGCTCAGATTGCAGCAATGGAAATCT CCCGGGGCAACCACAGAGATAACAAAGCCGTGATCCGCTATCTGCCTTGGCTCTACCATCCCCCCTCTGC AATGCAGCAAGGACCTAAAGAATTCATCGAGTGTGTCTCCCACATCCGACTGCTGTCCTGGCTACTTCTG GGGTCCCTCACGCATAATGCCGTCTGTCCCAATGCCTCCTCTCCCTGCCTCCCCATACCTCTGGATGCAG GCTCCCACATTGCAGACCACCTCATTGTTATCCTAATTGGATTTCCAGAGCAATCAAAGACGTGCGTGCT CCACATGTGCTCCCTCTTCCATGCGTTCATCTTCGCTCAGCTCTGGACCGTATACTGCGAGCAAAGTGCC GTGGCGACGAACGTCCAAAATCAGAACGAGTTCAGCTTCACAGCAATACTAACAGCGCTAGAGTTTTGGA GCAGGGTGACACCAAGCATCCTTCAGCTGATGGCCCACAATAAAGTGATGGTAGAGATGGTGTGTCTCCA TGTGATTAGTTTAATGGAGGCCTTGCAAGAATGCAATTCAACCATTTTTGTCAAGCTGATCCCTATGTGG TTACCAATGATTCAGTCCAATACCAAGTACTTATCCGCCGGCCTCCAGCTTCGTCTCCAGGCCATTCAGA ACAACGTGAACCACCACAGCCTAAGGACGCTGCCCGGCTCGGGCCAGAGCAGTGCTGGCCTAGCAGCCCT CCGGAAATGGCTGCAGTGCACCCAGTTCAAAATGGCCCAGGTGGAGATCCAGTCCTCAGAAGCAGCTTCT CAATTTTATCCTCTATGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001271253
Insert Size	7578 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271253.1, NP_001258182.1
RefSeq Size	8314 bp
RefSeq ORF	7578 bp
Locus ID	314401

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