Cul9 (NM_001191582) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Parc; RGD1562008
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217773 representing NM_001191582 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTAGGAGAACAGCGAGCAGGGGACCTTATGGTACCCTTGGGGCCTCGGCTGCAGGCATACCCTGAAG AACTCATTCGGCAGAGACCTGGACATGACGGCCATCCTGAATACCTGATCCGTTGGCGTGTCCTCAAGTG TAGCGAAGAGGGCAAAGTGAGCTTAGAGGAGGAGAAGACAGAGTACCTCCTCATGTGGCTGTCAGCCCCA GAGGTCTATGCCAACTGCCCCATGCTACTTCGGGAACGATCAGTGTCTAAAGGCCCACAGTACGAACCCA CCGGAGAGGTGGGGAGCTTCCCACGAGACCCAGGAGGCCTGGACGAGTTGGCAATGGCAGAGATGGAGGA CGATGTGCGGGCGCTGGTGCGCAGGGCTGCCAGGCAGCTGGCAGAAGGTGGGGCCTCCAACCTGACGGTT GCTGTGCTTCATACCATCCACGTGCTCAGTGCCTATGCCAGCATTGGGCCTCTCACCGGGGTCTTCAGGG AGACAGGAGCCCTGGATCTGCTAATGCACATGCTGTGCAGTCCGGAGCCTCAGATCCGTCGGAGTGCAGG CAAGATGCTACAGGCTCTGGCAGCCCATGATGCTGGGAGCCGGGCTCACGTCCTTCTGTCACTGAGCCAA CATGAGGGCATCGAGCAACACATGGATTTTGACAGCCGCTACACTTTACTGGAGCTGTTCGCAGAGACCA CGTCCACGGAGGAGCACTGCATGGCCTTGGAGGGCATCCACCTTCCTCAGCTCCCTGGGAAGCTGCTCTT CTCCCTGGTGAAACGCTACCTATGTGTCACGTCCCTGCTGGATCAGCTGAATAACAGCCCAGAGTCAGGA GCTGGAGACCAGAGCTCTCCGTGTCTCCCAAAGGAGAAGAGTCGGGGACAGCGGGAGCTGGAGTTCAGTA TGGCGGTGGGCAACCTCATTTCAGAGCTGGTGCGGAGCATGGGCTGGGCGCGGAACCTCAGTGAGCAGGG CGCCTTGCCGCCCCGGCCTTCCCGGTCCATCTTCCAGCCGTGCCTCTCAGGCCCTCCACTGCTGCTGCCC ACCATCGTCACTACGCCCAGGAAGCCAGGACGGGCCTTCCGCGGCCGTTCTGAATTTTCCAGCCGCAGTG GTTACGGTGAATATGTTCAGCAGACGGTGCAGCCCGGCATGCGCGTTCGCATCCTGGATGACTACGAAGA GATCAGTGCTGGGGACGAGGGCGAGTTCCAGCAGAGCAACAATGGCGTGCCTCCTGTGCAGGTCTTCTGG CAGTCAACCGGCCGCACCTACTGGGTCCACTGGCACATGCTAGAGATCCTGGGGCCTGAGGAAGCCACTA GGGAGCCAGCATCAGCAGCTGTGGAGAAGGGAGCAGGGGCAACTGCCCTGGGCACAGCGTGTCCCTCCTG GGACAGGAAGCCAGCGCATGGCCTCTACTCACTACCGTATCTACAGCCCGAGTCCCAGAAGAGCGAGGAG CCAGGGTACCTGACCCAGGGAGAGTGGTGGGAGCTACTTTTCTTCATCAAGAAGTTGGACTTGTGTGAGC AGCAGCCAATTTTCCAGAATCTGCATGAGAAACTGGATAAGGAGGCCGTGGGAGAAAAGACTCTGGGAGA GATCTCTGTGCCCATAGAGATGGCTGAGGGTGTGCTGCAGGTTCTCAGTAACCGCTTTGAGGGCAGCAAC CTCTGTGACCTGCTCAACTCCCACATCTACACCAAGTACGGACGGCTGCCCAAGGAACCAGGGCGCTTAC CGCCTTCACGGGGTCACTCCTGTACCTCCGAGCCAGAGGAGGGGTCGGAGTCAGCCGCCAGCTTCTCAGA GAAGGAAGAGTCCCCAAAGGCAGAGGCGGAGCCCGCAGGCGCAAAGCCGGAGTCCCCCAAAGCACAGAGC GACTCCCAGCTGTTCAATCAGCTTCTGGTGACTGAGGGGATGGCTCTGGCCCCTGAAACACAGGCGGCAG CTACTGAAATGGCCAGAACCTTGCGGGGTCCCGGTCCACGCAGCTCCCTGGATCAGCATGTGGCAGCCGT CTTAGCCACTGTGCAGATCTCCAGCCTGGATACAAACCTGCAGCTTTCAGGGCTCGGTGCCCTCTCCCAG GCGGTGGAGGAGGTCACTGAGCGGGACCACCCTCTGGTCCGTCCTGACAGATCCTTGAGAGAGAAGCTCG TGAAGACCCTGGTGGATTTGCTGACCAACCAGGTGGGAGAGAAGATGGTGGTGGTGCTGGCCCTGCGCCT CCTCTACCTGCTCATGACCAAGCATGAATGGCGTCCGCTCTTTGCCAGGGAGGGTGGCATCTACGCTGTG CTGATCTGCATGCAAGAATACAAGACATCTGTCTTGGTGCAGCAGGCGGGACTGGCAGCGCTAAAGATGC TAGCCATTGCTAATTCCTCCGAGGTGCCCACTTTTGTCACCGGCCGAGATTCCATCCAGCCTCTGTTTGA TACCCAGATGACCAGAGAGATCTTTGCCAGCATTGACTCTGCCACACGTCCAGGCTCTGAGAGCTTGCTC CTCAGTGTACCCGCAGCCGTGGTCCTGATGCTGAATACCGAGGGGTGCTCCTCTGCAGTGAGAAATGGCC TGCTGCTACTCAACCTGCTTCTGTGTAACCACCACACTCTGGGAGACCAGATCATAACCCAGGAGCTGAG AGACACCTTGTTCAGGCACTCAGGGATAGCGCCGGGGACAGAACCAATGCCCACCACACGCACCATCCTC ATGATGCTTCTCAGTCGCTACTCAGAGCCTTGGGGAAGTCCTGAGCGAGCAGCACTGGAGACCCCAAGCA CCCAGGGTCAGGATGGGTCCCCTGAGTCACTCATCCGATCTCTGGTGGGGGACCTGTCAGCAGAACTCTT CCTGGACTTGGAGCGTGTTCTGTGCCACGAAGGCAGCCCTCAAGGTGCCATGACGCCCCTCCTCAAGCGC CTCCAGCAGGAAACACAGCCTTTCCTTCTGTTGCTGCGGACTCTAGATGCCCCTGGGCCCAACAGAGCTC TGCTGCTCACCGTACTCAGGGTCATGACACGACTACTGGATTATCCTGAGACAATGGTTCTCCCCTGGCA CGAGGTCTTGGAGCCCTGCCTCAACTGCCTGAATGGGCCAAGCAGTGACTCTGAGGTTGTCCAGGAACTG ACCTGCTTCCTGCACCGCCTGGCTCTGATGCACAAAGACTATGCGGTAGTGCTTTGCTGCCTGGGAGCCA AAGAAGCCCTCTCCAAAGTCCTGGACAAGCACTCCACTCAGCTCCTGCTGGCCTCGGAGCTCCGCCACCT GGTGACGGAGTGCGAGAAGTACGCCCAGCTCTACACTAACCTCACCTCGAGCCTCCTGGCTGCCTGCATC CAGATGGTGCTGGGCCAGATTGAAGACCACAGGCGAACCTACAAGCCCATCAACATCCCCTTCTTTGATG TGTTCCTCAGGCATCTCTGCCAGGGCTCCAGTGTGGAAGTGAAGGAAGACAGGTGCTGGGAGAAGGTGGA GGTGTCCTCCAATCCACACCGGGCCAGCAAGCTGACGGACCGCAATCCCAAGACCTACTGGGAGTCCAAC GGCAGCACTGGCTCCCACTCCATCACCTTGCACATGCACCGTGGTGTTCTTATTAGGCAGCTGACCTTGC TTGTGGCCAGTGAGGATTCAAGTTACATGCCAGCCAGGGTGGTGGTGTTTGGGGGTGACAGTGTTGGCTG CATCAGCACCGAACTTAACACGGTGAATGTGGTGCCCTCTGCCAGCCGGGTGACACTCCTGGAGAACCTC AGCCGCTTCTGGCCCATCATCCAGATCCGAATAAAGCGCTGTCAGCAGGGCGGCATCGACACTCGAGTTC GGGGTGTGGAGGTGCTGGGCCCCAAGCCCACCTTCTGGCCATTGTTCCGGGAGCAACTCTGCCGACGGAC TTGTCTCTTCTACACAATTCGGGCACAAGCATGGAGCCGGGACATAGCAGAGGACCGCCAGCGCCTCCTT CAGCTCTATCCTAGACTGAACAGAGTTTTACGCCATGAACAGAATTTTGCAGATCGCTTCCTCCCTGATG ATGAGGCTGCCCAAGCACTGGGCAAAACCTGCTGGGAGGCGCTGGTCAGCCCCCTGGTGCAGAACATCAC CTCTCCCGATGCAGGGGGTGTGAGTTCCCTGGGATGGCTGCTGGACCAGTACCTGGAGCACAGGGAGAAA GCGAGGAGCCGCCAGGGTCAGGCAGCATCCTTTGCTTCTCAAGTTCGGCGCTTATGCCACTTGCTGGTAC ACGTGGAAGCCCCTCCTGGGCCTTCTCCTGAGCCGTCCTCGCAGCCCCTCAGCAAGAGCAGTAAGAGTCA GGACGGAAGCCCGGCCCCGGCCCCCGTCCGCCCCAGCACCACCCTTAGGAACATCACCCAGTGCTGGCTG GCGGTGGTGCAGGGACAGGCCAGCAGATTCCTGGCCTCAGCCTGGAGGGCCTCGGACTTCGTGCCTCGCT ACTGTGATCTCTACGAGCGCTTGCAAAGAGCCGGCTCAGAGCTGTTCGGGCCGCGGGCAGCCTTCACCCT GGCCCTGCGCAGCGGCTTCTCTGGGGCTCTGCTGCAGCAGTCCTTCCTCACTGCTGCTCACATAAGTGAG CAGTTTGCGAGGCACATTGACCAGCAGATCCAGGGTGGTTTGCTTGGTGGAGCTGCTGGGGTGGAAATGC TGGGGCGGCTTCAGCGGCACCTGGAGCCCATCATGGTCCTTTCTGGCCTAGAGCTGGCCACTACTTTTGA GCACTTCTATCAGTACTACATGGCTGACAGGCTCCTGAGCTTGGGCTCGAGCTGGCTGGAGGGGGCCGTG CTGGAGCAGATTGGCCTCTGCTTCCCCAACCGCCTCCCCCAGCTGATGCTGCAGAGCCTGCGCACCTCAG AGGAGCTGCAGCGTGGGTTCCTCCTCTTCCAGCTCCAGCAGCTCGACCGGCAGCTCTTGGAGCAGGGGGA GCAGGAGGAATGGAAGCTAGAGAGACTGGAGGAAGAAGACAGGGGACAGGAGACTGGAAGAGAACTCCTT ACAGAAGACCCAGGACCAGCCATTTCTGTCCTGGTCCTGTCACCGCGCTGCTGGCCGGTCTCCCCACTGT GCTACCTGCACCAGCCCAGGAAGCACCTTCCCACGGAGTTCTGTGATGCCCTTGACGGCTTCTCCAGCTT CTACAGCCACAGTCAGCACTACCCAGTCTTGGACATGGGACCACATCGGCGACTTCAGTGGACATGGCTG GGCCGGGCTGAGCTGCAGTTTGGGGACCAGACCCTGCATGTGTCCACTGTGCAGATGTGGCTGCTGCTGA ATTTCAACCAGACAGAGGAGGTGTCAGTAGAGATCTTGTTGAGGAACTCTGGCCTCTCCCCTGAGCTCCT GCACCAGGCCCTTCTGCCGCTCACCTCAGACAGTGGTCCCTTAACACTGGAGGAGGCTCAGGACTTTCCA CAGGGGGGGGTGCTGCGGCTTCGTGAGCCTAGGTCCCAGACCCATGAAGAGGTCCTGTGGCTGATACCTC CCCAGACGTATCTCAGTGTGGAAAAGGATGAGGGCCGAACCTTGGAGCAGAAGAGGAACCTCTTGAGCTG CCTTCTCGTACGGATACTCAAAGCCCACGGGGAGAAAGGCCTGCACATCGATCAGCTGGTTTGTCTGGTT CTGGAGGCCTGGCAGAAGGGGCCAAATCCACCGGGGCGCCTGGGCCGCTCTGCTGCAGTGGGCGTGGCCT GTTCCAGCACTGACGTCCTCTCTTGTATCCTGCATCTCCTGGGCCAGGGCTACGTGGAGCGGAGAGATGA CCGACCTCAGGTCCTGATGTATGCCACCCCAGAGCCCATGGGGCACTGCCGGGGCCAGGCAGATGTCCCC TTTTGTGGCAACCAGAGCACGGAGACCTCCAAACCTAGCCCAGAAGCTGTGGTGGCCCTGGCCTCTCTGC AGCTGCCGGCAGGCCGGACCATGAGTCCCCAGGAGGTAGAAGGATTGATGGAGCAGACAGTGCGGCAGGT GCAGGAGACGCTGAACTTGGAGCCCGATGTGGCTCAGCACCTTCTGGCTTATTCCCACTGGGGCACGGAG CAGCTGCTACAAAGCTACAGCGAGGATCCCGAGCCACTGCTGCTCGCCGCAGGGCTGCGAGTGCCCCAGC CCCAGGAGGTGCCCGCTCGCCCTGACCAGTGCCCCGTGTGCATCAGCCCCCTAGGGCCCAATGACGACTC GCCCTCCCTCTGCTGCCTGCACTGCTGCTGTAAGTCTTGCTGGAACGAGTACCTGACGACTCGGATAGAG CAGAACTTTGTGCTGAACTGTACCTGCCCCATCGCTGACTGTCCCGCCCAGCCCACGGGAGCCTTCATCC GAAACATTGTCTCTTCCCCAGAGGTCATCTCCAAGTATGAGAAGGCCCTTCTGCGAGGTTATGTGGAGAG CTGCTCCAACCTGACATGGTGCACCAACCCTCAGGGCTGCGACCGTATCCTGTGCCGCCAGGGCCTGGGC AGTGGGACCACCTGCTCTAAGTGCGGCTGGGCCTCTTGCTTCAACTGTAGTTTTCCTGAGGCGCACTATC CTGCCAGCTGTGGCCACATGTCCCAGTGGGTTGACGATGGCGGCTACTATGACGGCATGAGCGTGGAGGC CCAGAGCAAGCACCTGGCCAAGCTCATCTCCAAACGCTGTCCCAGCTGTCAGGCGCCTATCGAGAAGAAT GAGGGGTGTCTACACATGACCTGTGCAAGATGTAACCATGGGTTCTGCTGGCGCTGCCTCAGGTCCTGGA AGCCAAGCCACAAGGACTACTACAACTGCTCTGCCATGGTAAGCAAAGCAGCTCGCCAGGAGAAGCGGTT TCAGGACTATAACGAGAGGTGTACCTTCCACCACCAGGCCCGGGAGTTTGCTGTCAACCTGCGGAACCAG GCCTCTGCCATCCATGAGGTGCCTCCGCCCAAATCCTTCACCTTCCTGCAAGACGCCTGCCGGGCCCTCG AGCAGGCTCGAAAGGTGCTGGCCTACGCCTGTGTCTATAGCTTCTACAGCCAGGGCACGGAGTACATGGA TGTGGTGGAGCAGCAGACGGAGAACCTGGAGCTGCACACGAATGCCCTGCAGATCCTCCTCGAGGAGACC TTGCTGCGCTGCCAGGACCTGGCCTCCTCCCTGCGCCTCCTGCGGGCTGACTGCCTCAGCACAGGCACGG AGTTGCTCCGGAGGATCCAGGAGAGGCTGTTGGCCATCCTACAGCACTCGACCCAGGACTTCCGGGTCGG GCTGCAGAGTCCCTCAGTAGAGACCCGGGAGGTGAAAGGGTCCAACGTGCCCAGTGACCAGCCCCAGGGC TCCTCAGGACTGGAAGTGGAAGACGAGGAGGAAGAAGAGGAAGAGGAGGAGGAGGAGGATGTACCTGAGT GGCAGCACGAGTTTGATGAGGAACTAGACAATGACAGCTTCTCCTACGATGAGGAGTCTGAGAACCTGGA CCGAGAGACCTTCTTCTTTGGCGATGAAGATGACGATGATGAGTCCTATGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191582
Insert Size	7545 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191582.1, NP_001178511.1
RefSeq Size	7669 bp
RefSeq ORF	7545 bp
Locus ID	316228

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